Molecular epidemiological characteristics of Brucella spp. isolated from human and animal brucellosis

DOI: 10.18805/ijar.B-978    | Article Id: B-978 | Page : 399-403
Citation :- Molecular epidemiological characteristics of Brucella spp. isolated from human and animal brucellosis.Indian Journal Of Animal Research.2019.(53):399-403
Tülin Güven Gökmen, Togrul Nagiyev, Süleyman Aslan, Melda Meral, Pinar Etiz, Akgün Yaman and Fatih Köksal tulinguven01@hotmail.com
Address : Department of Microbiology, Medical Faculty, Cukurova University, Adana, Turkey.
Submitted Date : 1-05-2018
Accepted Date : 17-10-2018

Abstract

Brucellosis is the most common zoonotic disease in the Mediterranean region where Turkey is located. Therefore, particularly in this region, it is crucial to follow molecular surveillance methods for the control and eradication of this disease agent, which threatens both human and animal life. We have conducted a molecular-based surveillance study for detecting the origin of the possible reservoir in human brucellosis and transmission of Brucella spp. between humans and ruminants. For the detection of clonal relationships, we isolated Brucella spp. from human and ruminants diagnosed with brucellosis and evaluated them by PFGE and MLVA methods. We found 10 subclusters in PFGE and 22 subclusters in MLVA-16. Human and ruminant isolates were found to co-exist in MLVA-16-m1 and PFGE-a8, a9, a10 subcluster by both methods. In conclusion, the results revealed that these subcluster isolates that co-exist some ruminant and human isolates can be more effective in infecting humans.

Keywords

Brucella MLVA Molecular surveillance PFGE.

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