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Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Research Article

Indian Journal of Animal Research, volume 52 issue 10 (october 2018) : 1428-1432

Effects of 17á-ethinylestradiol-based contraceptives on ovarian tissue using histopathological examination and FSH, LH, CYP1A, and CYP3A expressions using qRT-PCR in the ovaries and brain tissues of common carp (Cyprinus carpio L.)

Harun Arslan
1Department of Basic Sciences, Faculty of Fisheries, Ataturk University, Yakutiye, 25240, Erzurum, Turkey.
Cite article:- Arslan Harun (2018). Effects of 17á-ethinylestradiol-based contraceptives on ovarian tissue using histopathological examination and FSH, LH, CYP1A, and CYP3A expressions using qRT-PCR in the ovaries and brain tissues of common carp (Cyprinus carpio L.). Indian Journal of Animal Research. 52(10): 1428-1432. doi: 10.18805/ijar.B-959.

ABSTRACT

In this study, the main aim was  to identify the effects of exposure to 17á-ethinylestradiol-based contraceptives on the ovaries and brain tissues of common carp. For this purpose, histopathological changes were evaluated in ovaries after 21 days of contraceptive exposure. In addition, the expression levels of the CYP1A, CYP3A, FSH, and LH genes were measured in contraceptive-exposed brain tissue and ovaries respectively, using qRT-PCR (p<0.05, p<0.01, and p<0.001, respectively). No histopathological lesions, including inflammation, degeneration, or necrosis in the evaluated ovarian tissue of common carp were found after both high and low levels of exposure to contraceptives. Therefore, it is concluded that these contraceptives were highly dangerous to the reproduction system of common carp; the accumulation of contraceptive waste in the water supply could adversely affect and threat the common carp population. This may indirectly also affect other animals.

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