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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 10 (october 2019) : 1298-1303,   Doi: 10.18805/ijar.B-949

Comparison of vaginal microbial community structure of beef cattle between luteal phase and follicular phase

J
Jun Wang
C
Chang Liu
L
Lucky T. Nesengani
Y
Yongsheng Gong
Y
Yujiang Yang
L
Lianyu Yang
W
Wenfa Lu
1<div style="text-align: justify;">College of Animal Science and Technology, Jilin Agricultural University, Changchun-130 118, China.</div>
Cite article:- Wang Jun, Liu Chang, Nesengani T. Lucky, Gong Yongsheng, Yang Yujiang, Yang Lianyu, Lu Wenfa (2018). Comparison of vaginal microbial community structure of beef cattle between luteal phase and follicular phase. Indian Journal of Animal Research. 53(10): 1298-1303. doi: 10.18805/ijar.B-949.

ABSTRACT

The aim of the current study was to evaluate the vaginal microbial community structure in beef cattle between follicular and luteal phase. Fifteen healthy beef cattle were subjected to an estrus synchronization protocol, and vaginal samples were collected in the follicular and luteal phases. Polymerase chain reaction (PCR) followed by denaturing gradient gel electrophoresis (PCR-DGGE) and real-time PCR were used to determine the vaginal community structure. The results showed that the vaginal microflora of beef cattle mainly comprised of Aerococcus vaginalis, Aerococcus viridans, Haemophilus somnus, Streptococcus pluranimalium, Psychrobacter marincola, Escherichia coli, and Sphingomonas roseiflava. There was no significant difference in the total number of bacteria in the vaginas of beef cattle between the follicular and luteal phase. The quantity of vaginal Streptococcus spp. in the luteal phase was significantly higher than in the follicular phase, while the number of vaginal Lactobacillus spp. in the follicular phase was significantly higher than in the luteal phase. The results indicated that there was moderate significant difference in the structure of the vaginal microbial community in beef cattle between the luteal phase and follicular phase.

REFERENCES

  1. Chen,Y., Penner,G.B., Li,M., Oba,M. and Guan,L.L.(2011). Changes in bacterial diversity associated with epithelial tissue in the beef cow rumen during the transition to a high-grain diet. Appl Environ Microbiol, 77: 5770-5781.
  2. Cielesh,M.E., McGrath,B.M., Scott,C.J., Norman,S.T. and Stephen,C.P.(2017). The localization of kisspeptin and kisspeptin receptor in the canine ovary during different stages of the reproductive cycle. Reprod Domest Anim, 52: 24-28. 
  3. Clarke,H., Dhillo,W.S. and Jayasena,C.N.(2015). Comprehensive Review on Kisspeptin and Its Role in Reproductive Disorders. Endocrinol Metab (Seoul), 30: 124-141.
  4. Clarke,S.A. and Dhillo,W.S.(2016). Kisspeptin across the human lifespan:evidence from animal studies and beyond. J Endocrinol, 229: R83-98.
  5. El-Hayek,S. and Clarke,H.J.(2016). Control of Oocyte Growth and Development by Intercellular Communication Within the Follicular Niche. Results Probl Cell Differ, 58: 191-224.
  6. Gelsomino,A., Keijzer-Wolters,A.C., Cacco,G. and van Elsas,J.D.(1999). Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis. J Microbiol Methods, 38: 1-15.
  7. Lamont,R.F., Sobel,J.D., Akins,R.A., Hassan,S.S., Chaiworapongsa,T., Kusanovic,J.P. and Romero,R.(2011). The vaginal microbiome: new information about genital tract flora using molecular based techniques. BJOG: International Journal of Obstetrics & Gynaecology, 118: 533-549.
  8. Machado,V.S., Oikonomou,G., Bicalho,M.L, Knauer,W.A., Gilbert,R. and Bicalho,R.C.(2012). Investigation of postpartum dairy cows’ uterine microbial diversity using metagenomic pyrosequencing of the 16S rRNA gene. Vet Microbiol, 159: 460-469.
  9. Malinen,E., Kassinen,A., Rinttila,T. and Palva,A.(2003). Comparison of real-time PCR with SYBR Green I or 5'-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria. Microbiology, 149: 269-277.
  10. Otero,C., Sliva de Ruiz,C., lbanez,R., Wilde,O.R., de Ruiz Holgado,A.A.P., and Nader-Macias,M.E.(1999). Lactobacilli and Enterococci isolated from the bovine vaginal during the estrouscycle. anaerobe, 5: 305-307. 
  11. Otero,C., Saavedra,L., Silva de Ruiz,C., Wilde,O., Holgado,A.R. and Nader-Macias,M.E.(2000). Vaginal bacterial microflora modifications    during the growth of healthy cows. Lett Appl Microbiol, 31: 251-254.
  12. Rimon-Dahari,N., Yerushalmi-Heinemann,L., Alyagor,L. and Dekel,N.(2016). Ovarian Folliculogenesis. Results Probl Cell Differ, 58: 167-190.
  13. Santos,T.M. and Bicalho,R.C.(2012). Diversity and succession of bacterial communities in the uterine fluid of postpartum metritic, endometritic and healthy dairy cows. PLoS One, 7: e53048.
  14. Vitali,B., Pugliese,C., Biagi,E., Candela,M., Turroni,S., Bellen,G., Donders,G.G. and Brigidi,P.(2007). Dynamics of vaginal bacterial communities in women developing bacterial vaginosis, candidiasis, or no infection, analyzed by PCR-denaturing gradient gel electrophoresis and real-time PCR. Appl Environ Microbiol, 73: 5731-5741.
  15. Wang,J., Sun,C., Liu,C., Yang,Y. and Lu,W.(2016). Comparison of vaginal microbial community structure in healthy and endometritis dairy cows by PCR-DGGE and real-time PCR. Anaerobe , 38: 1-6.
  16. Wang,Y., Ametaj,B.N., Ambrose,D.J. and Ganzle,M.G.(2013). Characterisation of the bacterial microbiota of the vagina of dairy cows and isolation of pediocin-producing Pediococcus acidilactici. BMC Microbiol, 13: 19.
  17. Xiao,Y., Ni,Y., Huang,Y., Wu,J., Grossmann,R. and Zhao,R.(2011). Effects of kisspeptin-10 on progesterone secretion in cultured chicken ovarian granulosa cells from preovulatory (F1-F3) follicles. Peptides, 32: 2091-2097.
  18. Yu,Z. and Morrison,M.(2004). Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis. Appl Environ Microbiol, 70: 4800-4806.
  19. Zhao,J., Wang,J., Yang,Y.J., Li,X.N. and Sun,C.T.(2014). In vitro assessment of probiotic properties of lactic acid bacteria isolated from vaginas of healthy cows. Indian Journal of Animal Research, 49: 355-359. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 10 (october 2019) : 1298-1303,   Doi: 10.18805/ijar.B-949

    Comparison of vaginal microbial community structure of beef cattle between luteal phase and follicular phase

    J
    Jun Wang
    C
    Chang Liu
    L
    Lucky T. Nesengani
    Y
    Yongsheng Gong
    Y
    Yujiang Yang
    L
    Lianyu Yang
    W
    Wenfa Lu
    1<div style="text-align: justify;">College of Animal Science and Technology, Jilin Agricultural University, Changchun-130 118, China.</div>
    Cite article:- Wang Jun, Liu Chang, Nesengani T. Lucky, Gong Yongsheng, Yang Yujiang, Yang Lianyu, Lu Wenfa (2018). Comparison of vaginal microbial community structure of beef cattle between luteal phase and follicular phase. Indian Journal of Animal Research. 53(10): 1298-1303. doi: 10.18805/ijar.B-949.

    ABSTRACT

    The aim of the current study was to evaluate the vaginal microbial community structure in beef cattle between follicular and luteal phase. Fifteen healthy beef cattle were subjected to an estrus synchronization protocol, and vaginal samples were collected in the follicular and luteal phases. Polymerase chain reaction (PCR) followed by denaturing gradient gel electrophoresis (PCR-DGGE) and real-time PCR were used to determine the vaginal community structure. The results showed that the vaginal microflora of beef cattle mainly comprised of Aerococcus vaginalis, Aerococcus viridans, Haemophilus somnus, Streptococcus pluranimalium, Psychrobacter marincola, Escherichia coli, and Sphingomonas roseiflava. There was no significant difference in the total number of bacteria in the vaginas of beef cattle between the follicular and luteal phase. The quantity of vaginal Streptococcus spp. in the luteal phase was significantly higher than in the follicular phase, while the number of vaginal Lactobacillus spp. in the follicular phase was significantly higher than in the luteal phase. The results indicated that there was moderate significant difference in the structure of the vaginal microbial community in beef cattle between the luteal phase and follicular phase.

    REFERENCES

    1. Chen,Y., Penner,G.B., Li,M., Oba,M. and Guan,L.L.(2011). Changes in bacterial diversity associated with epithelial tissue in the beef cow rumen during the transition to a high-grain diet. Appl Environ Microbiol, 77: 5770-5781.
    2. Cielesh,M.E., McGrath,B.M., Scott,C.J., Norman,S.T. and Stephen,C.P.(2017). The localization of kisspeptin and kisspeptin receptor in the canine ovary during different stages of the reproductive cycle. Reprod Domest Anim, 52: 24-28. 
    3. Clarke,H., Dhillo,W.S. and Jayasena,C.N.(2015). Comprehensive Review on Kisspeptin and Its Role in Reproductive Disorders. Endocrinol Metab (Seoul), 30: 124-141.
    4. Clarke,S.A. and Dhillo,W.S.(2016). Kisspeptin across the human lifespan:evidence from animal studies and beyond. J Endocrinol, 229: R83-98.
    5. El-Hayek,S. and Clarke,H.J.(2016). Control of Oocyte Growth and Development by Intercellular Communication Within the Follicular Niche. Results Probl Cell Differ, 58: 191-224.
    6. Gelsomino,A., Keijzer-Wolters,A.C., Cacco,G. and van Elsas,J.D.(1999). Assessment of bacterial community structure in soil by polymerase chain reaction and denaturing gradient gel electrophoresis. J Microbiol Methods, 38: 1-15.
    7. Lamont,R.F., Sobel,J.D., Akins,R.A., Hassan,S.S., Chaiworapongsa,T., Kusanovic,J.P. and Romero,R.(2011). The vaginal microbiome: new information about genital tract flora using molecular based techniques. BJOG: International Journal of Obstetrics & Gynaecology, 118: 533-549.
    8. Machado,V.S., Oikonomou,G., Bicalho,M.L, Knauer,W.A., Gilbert,R. and Bicalho,R.C.(2012). Investigation of postpartum dairy cows’ uterine microbial diversity using metagenomic pyrosequencing of the 16S rRNA gene. Vet Microbiol, 159: 460-469.
    9. Malinen,E., Kassinen,A., Rinttila,T. and Palva,A.(2003). Comparison of real-time PCR with SYBR Green I or 5'-nuclease assays and dot-blot hybridization with rDNA-targeted oligonucleotide probes in quantification of selected faecal bacteria. Microbiology, 149: 269-277.
    10. Otero,C., Sliva de Ruiz,C., lbanez,R., Wilde,O.R., de Ruiz Holgado,A.A.P., and Nader-Macias,M.E.(1999). Lactobacilli and Enterococci isolated from the bovine vaginal during the estrouscycle. anaerobe, 5: 305-307. 
    11. Otero,C., Saavedra,L., Silva de Ruiz,C., Wilde,O., Holgado,A.R. and Nader-Macias,M.E.(2000). Vaginal bacterial microflora modifications    during the growth of healthy cows. Lett Appl Microbiol, 31: 251-254.
    12. Rimon-Dahari,N., Yerushalmi-Heinemann,L., Alyagor,L. and Dekel,N.(2016). Ovarian Folliculogenesis. Results Probl Cell Differ, 58: 167-190.
    13. Santos,T.M. and Bicalho,R.C.(2012). Diversity and succession of bacterial communities in the uterine fluid of postpartum metritic, endometritic and healthy dairy cows. PLoS One, 7: e53048.
    14. Vitali,B., Pugliese,C., Biagi,E., Candela,M., Turroni,S., Bellen,G., Donders,G.G. and Brigidi,P.(2007). Dynamics of vaginal bacterial communities in women developing bacterial vaginosis, candidiasis, or no infection, analyzed by PCR-denaturing gradient gel electrophoresis and real-time PCR. Appl Environ Microbiol, 73: 5731-5741.
    15. Wang,J., Sun,C., Liu,C., Yang,Y. and Lu,W.(2016). Comparison of vaginal microbial community structure in healthy and endometritis dairy cows by PCR-DGGE and real-time PCR. Anaerobe , 38: 1-6.
    16. Wang,Y., Ametaj,B.N., Ambrose,D.J. and Ganzle,M.G.(2013). Characterisation of the bacterial microbiota of the vagina of dairy cows and isolation of pediocin-producing Pediococcus acidilactici. BMC Microbiol, 13: 19.
    17. Xiao,Y., Ni,Y., Huang,Y., Wu,J., Grossmann,R. and Zhao,R.(2011). Effects of kisspeptin-10 on progesterone secretion in cultured chicken ovarian granulosa cells from preovulatory (F1-F3) follicles. Peptides, 32: 2091-2097.
    18. Yu,Z. and Morrison,M.(2004). Comparisons of different hypervariable regions of rrs genes for use in fingerprinting of microbial communities by PCR-denaturing gradient gel electrophoresis. Appl Environ Microbiol, 70: 4800-4806.
    19. Zhao,J., Wang,J., Yang,Y.J., Li,X.N. and Sun,C.T.(2014). In vitro assessment of probiotic properties of lactic acid bacteria isolated from vaginas of healthy cows. Indian Journal of Animal Research, 49: 355-359. 
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