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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 10 (october 2019) : 1292-1297,   Doi: 10.18805/ijar.B-943

Porcine somatic cell nuclear transfer donor sources affect transplant success: A meta-analysis

Z
Zhenhua Guo
L
Lei Lv
D
Di Liu
Z
Zhongqiu Li
1<div style="text-align: justify;">Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Animal Husbandry Research Institute, Key Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture, No. 368 Xuefu Road, Harbin 150 086, P. R. China.</div>
Cite article:- Guo Zhenhua, Lv Lei, Liu Di, Li Zhongqiu (2018). Porcine somatic cell nuclear transfer donor sources affect transplant success: A meta-analysis. Indian Journal of Animal Research. 53(10): 1292-1297. doi: 10.18805/ijar.B-943.

ABSTRACT

Herd boars, male domestic pigs used for stud, are economically important, and somatic cell nuclear transfer (SCNT) is a promising technology to expand herd boar yields. However, live births are dictated by donor cell source, and fetal donors may offer more advantages than adult donors. A meta-analysis was conducted to better understand how donor sources affect SCNT outcomes. Of the 1,431 records viewed, 10 were selected for review. Blastocyst formation rates, successful pregnancies, and live births were assessed to measure efficacy. SCNT blastocyst formation differed between adult and fetal donors among the studies. SCNT pigs had more malformed fetuses as well, which negatively affected the post-birth mortality. Organs of porcine fetuses are limited by deficiencies of maternal nutrient and growth hormones, which compromise post-birth adaptations. SCNT pregnancy success is neither determined by donor source nor by live births. Live births are also tied to donor age. Embryos from fetal donors are more frequently healthy likely due to less differentiation and less reprogramming of reconstructed embryos. Adult donors in contrast have more cell differentiation and as such accumulate more mutations and damage. This may reduce reconstructed embryo viability. Finally, SCNT efficiency may be improved with more in vitro passages, but more work is required to validate this concept.

REFERENCES

  1. Che L., Xu M., Yang Z., Xu S., Che L., Lin Y., Fang Z., Feng B., Li J., Chen D. and Wu. (2016). Detection of placental proteomes at different uterine positions in Large White and Meishan Gilts on gestational day 90. PLOS ONE, 11 (12): e0167799.
  2. Gade N.E., Nath A., Pratheesh M.D., Dubey P.K., Amarpal, Kumar G.S. and Sharma G.T. (2012). Stem cell therapy in animal sciences - a review. Agree Rev, 33 (2): 150-158.
  3. Galli C., Lagutina I., Crotti G., Colleoni S., Turini P., Ponderato N., Duchi R. and Lazzari G. (2003). Pregnancy: a cloned horse born to its dam twin. Nature, 424 (6949): 635.
  4. Hua Z., Xu G., Liu X., Bi Y., Xiao H., Hua W., Li L., Zhang L., Ren H. and Zheng X. (2016). Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig. Pol J. Vet Science, 19 (1): 205-12.
  5. Lal S.V., Singh S., Kumari R. and Kumar S. (2012). Molecular and epigenetic study of H19 gene in goat (Capra hircus). Indian J Anim Res, 46 (1): 15-21.
  6. Lan J., Lepikhov K., Giehr P. and Walter J. (2017). Histone and DNA methylation control by H3 serine 10/threonine 11 phosphorylation in the mouse zygote. EPIGENET CHROMATIN, 10: 5.
  7. Lee K., Hamm J., Whitworth K., Spate L., Park K.W., Murphy C.N. and Prather R.S. (2014). Dynamics of TET family expression in porcine preimplantation embryos is related to zygotic genome activation and required for the maintenance of Nanog. Dev Biol, 386 (1): 86-95.
  8. Liu T., Dou H., Xiang X., Li L., Li Y., Lin L., Pang X., Zhang Y., Chen Y., Luan J.,et al. (2015). Factors Determining the Efficiency of Porcine Somatic Cell Nuclear Transfer: Data Analysis with Over 200,000 Reconstructed Embryos. Cell Reprogram, 17 (6): 463-71.
  9. Liu Y., Ostrup O., Li J., Vajta G., Lin L., Kragh P.M., Purup S., Hyttel P. and Callesen H. (2012). Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin. Zygote, 20 (1): 61-6.
  10. Mehta P., Sharma A. and Kaushik R. (2017). Transgenesis in farm animals-A review. Agric Rev, 38 (2): 129-136.
  11. Oh H.J., Park J.E., Park E.J., Kim M.J., Kim G.A., Rhee S.H., Lim S.H., Kang S.K. and Lee B.C. (2014). Analysis of cell growth and gene expression of porcine adipose tissue-derived mesenchymal stem cells as nuclear donor cell. DEV GROWTH DIFFER, 56 (9): 595-604.
  12. Okae H., Matoba S., Nagashima T., Mizutani E., Inoue K., Ogonuki N., Chiba H.,et al. (2014). RNA sequencing-based identification of aberrant imprinting in cloned mice. HUM MOL GENET, 23 (4): 992-1001.
  13. Peat J.R. and Reik W. (2012). Incomplete methylation reprogramming in SCNT embryos. NAT GENET, 44 (9): 965-6.
  14. Salilew-Wondim D., Tesfaye D., Hossain M., Held E., Rings F., Tholen E., Looft C.,et al. (2013). Aberrant placenta gene expression pattern in bovine pregnancies established after transfer of cloned or in vitro produced embryos. PHYSIOL GENOMICS, 45 (1): 28-46.
  15. Wang Q.H., Peng Y., Cai X.Y., Wan M., Liu Y. and Wei H. (2015). Nucleus transfer efficiency of ear fibroblast cells isolated from Bama miniature pigs at various ages. J HUAZHONG U SCI-MED, 35 (4): 585-90.
  16. Wen D., Banaszynski L.A., Liu Y., Geng F., Noh K.M., Xiang J., Elemento O.,et al. (2014). Histone variant H3.3 is an essential maternal factor for oocyte reprogramming. P NATL ACAD SCI USA, 111 (20): 7325-30. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 10 (october 2019) : 1292-1297,   Doi: 10.18805/ijar.B-943

    Porcine somatic cell nuclear transfer donor sources affect transplant success: A meta-analysis

    Z
    Zhenhua Guo
    L
    Lei Lv
    D
    Di Liu
    Z
    Zhongqiu Li
    1<div style="text-align: justify;">Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Animal Husbandry Research Institute, Key Laboratory of Combining Farming and Animal Husbandry, Ministry of Agriculture, No. 368 Xuefu Road, Harbin 150 086, P. R. China.</div>
    Cite article:- Guo Zhenhua, Lv Lei, Liu Di, Li Zhongqiu (2018). Porcine somatic cell nuclear transfer donor sources affect transplant success: A meta-analysis. Indian Journal of Animal Research. 53(10): 1292-1297. doi: 10.18805/ijar.B-943.

    ABSTRACT

    Herd boars, male domestic pigs used for stud, are economically important, and somatic cell nuclear transfer (SCNT) is a promising technology to expand herd boar yields. However, live births are dictated by donor cell source, and fetal donors may offer more advantages than adult donors. A meta-analysis was conducted to better understand how donor sources affect SCNT outcomes. Of the 1,431 records viewed, 10 were selected for review. Blastocyst formation rates, successful pregnancies, and live births were assessed to measure efficacy. SCNT blastocyst formation differed between adult and fetal donors among the studies. SCNT pigs had more malformed fetuses as well, which negatively affected the post-birth mortality. Organs of porcine fetuses are limited by deficiencies of maternal nutrient and growth hormones, which compromise post-birth adaptations. SCNT pregnancy success is neither determined by donor source nor by live births. Live births are also tied to donor age. Embryos from fetal donors are more frequently healthy likely due to less differentiation and less reprogramming of reconstructed embryos. Adult donors in contrast have more cell differentiation and as such accumulate more mutations and damage. This may reduce reconstructed embryo viability. Finally, SCNT efficiency may be improved with more in vitro passages, but more work is required to validate this concept.

    REFERENCES

    1. Che L., Xu M., Yang Z., Xu S., Che L., Lin Y., Fang Z., Feng B., Li J., Chen D. and Wu. (2016). Detection of placental proteomes at different uterine positions in Large White and Meishan Gilts on gestational day 90. PLOS ONE, 11 (12): e0167799.
    2. Gade N.E., Nath A., Pratheesh M.D., Dubey P.K., Amarpal, Kumar G.S. and Sharma G.T. (2012). Stem cell therapy in animal sciences - a review. Agree Rev, 33 (2): 150-158.
    3. Galli C., Lagutina I., Crotti G., Colleoni S., Turini P., Ponderato N., Duchi R. and Lazzari G. (2003). Pregnancy: a cloned horse born to its dam twin. Nature, 424 (6949): 635.
    4. Hua Z., Xu G., Liu X., Bi Y., Xiao H., Hua W., Li L., Zhang L., Ren H. and Zheng X. (2016). Impact of different sources of donor cells upon the nuclear transfer efficiency in Chinese indigenous Meishan pig. Pol J. Vet Science, 19 (1): 205-12.
    5. Lal S.V., Singh S., Kumari R. and Kumar S. (2012). Molecular and epigenetic study of H19 gene in goat (Capra hircus). Indian J Anim Res, 46 (1): 15-21.
    6. Lan J., Lepikhov K., Giehr P. and Walter J. (2017). Histone and DNA methylation control by H3 serine 10/threonine 11 phosphorylation in the mouse zygote. EPIGENET CHROMATIN, 10: 5.
    7. Lee K., Hamm J., Whitworth K., Spate L., Park K.W., Murphy C.N. and Prather R.S. (2014). Dynamics of TET family expression in porcine preimplantation embryos is related to zygotic genome activation and required for the maintenance of Nanog. Dev Biol, 386 (1): 86-95.
    8. Liu T., Dou H., Xiang X., Li L., Li Y., Lin L., Pang X., Zhang Y., Chen Y., Luan J.,et al. (2015). Factors Determining the Efficiency of Porcine Somatic Cell Nuclear Transfer: Data Analysis with Over 200,000 Reconstructed Embryos. Cell Reprogram, 17 (6): 463-71.
    9. Liu Y., Ostrup O., Li J., Vajta G., Lin L., Kragh P.M., Purup S., Hyttel P. and Callesen H. (2012). Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin. Zygote, 20 (1): 61-6.
    10. Mehta P., Sharma A. and Kaushik R. (2017). Transgenesis in farm animals-A review. Agric Rev, 38 (2): 129-136.
    11. Oh H.J., Park J.E., Park E.J., Kim M.J., Kim G.A., Rhee S.H., Lim S.H., Kang S.K. and Lee B.C. (2014). Analysis of cell growth and gene expression of porcine adipose tissue-derived mesenchymal stem cells as nuclear donor cell. DEV GROWTH DIFFER, 56 (9): 595-604.
    12. Okae H., Matoba S., Nagashima T., Mizutani E., Inoue K., Ogonuki N., Chiba H.,et al. (2014). RNA sequencing-based identification of aberrant imprinting in cloned mice. HUM MOL GENET, 23 (4): 992-1001.
    13. Peat J.R. and Reik W. (2012). Incomplete methylation reprogramming in SCNT embryos. NAT GENET, 44 (9): 965-6.
    14. Salilew-Wondim D., Tesfaye D., Hossain M., Held E., Rings F., Tholen E., Looft C.,et al. (2013). Aberrant placenta gene expression pattern in bovine pregnancies established after transfer of cloned or in vitro produced embryos. PHYSIOL GENOMICS, 45 (1): 28-46.
    15. Wang Q.H., Peng Y., Cai X.Y., Wan M., Liu Y. and Wei H. (2015). Nucleus transfer efficiency of ear fibroblast cells isolated from Bama miniature pigs at various ages. J HUAZHONG U SCI-MED, 35 (4): 585-90.
    16. Wen D., Banaszynski L.A., Liu Y., Geng F., Noh K.M., Xiang J., Elemento O.,et al. (2014). Histone variant H3.3 is an essential maternal factor for oocyte reprogramming. P NATL ACAD SCI USA, 111 (20): 7325-30. 
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