Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 54 issue 2 (february 2020) : 234-238

Pathomorphology of experimental Streptococcus iniae infection in tilapia (Oreochromis niloticus)

A.E. Dönmez, I. Cengizler
1Department of Aquaculture, Faculty of Fisheries, Mersin University, 33160, Mersin, Turkey.
Cite article:- Dönmez A.E., Cengizler I. (2018). Pathomorphology of experimental Streptococcus iniae infection in tilapia (Oreochromis niloticus). Indian Journal of Animal Research. 54(2): 234-238. doi: 10.18805/ijar.B-908.
In the present study, an experimental infection with Streptococcus iniae was conducted with tilapia (Oreochromis niloticus). The bacterial agent was injected to the fish by intraperitoneal inoculation at a dose of 1 × 103 cfu / mL. Grossly, haemorrhages on tail and pectoral fins, hyperaemic and prolapsed anus, darkening and bleeding in the gills and exophthalmos in the eyes were identified in fish. Multifocal whitish foci in the liver, spleen and kidneys was observed with ascites. Histopathological changes of tissues of tilapia were studied and evaluated. Gill tissues of infected fish showed intense lymphocyte and macrophage infiltrations. In addition, epithelial cell separation and lamellar fusion with edema, lamellar curling and aneurysms were detected in gill tissue. Sinusoidal dilatation, lymphocyte infiltrations, congestion and degeneration in liver hepatocyte cells have been identified. In kidney degeneration of glomerulus and tubule cells and dense lymphocyte infiltrations were observed. Dense bacterial clusters were observed in the sinusoids of the spleen, while lymphocytes and macrophage infiltrations were found in the heart and intestinal tissues.
  1. Aamri, F.E., Padilla, D., Acosta, F., Caballero, M.J., Roo, J., Bravo, J., Vivas, J. Real, F. (2010). First report of Streptococcus iniae in red porgy (Pagrus pagrus, L.). Journal of Fish Diseases, 33: 901–905.
  2. Abdullah, S., Omar, N., Yusoff, S.M., Obukwho, E.B., Nwunuji, T.P., Hanan, L., Samad, J. (2013). Clinicopathological features and immunohistochemical detection of antigens in acute experimental Streptococcus agalactiae infection in red tilapia (Oreochromis spp.). Springerplus, 2: 286. DOI:10.1186/2193-1801-2-286.
  3. Agnew, W., Barnes, A.C. (2007). Streptococcus iniae: An aquatic pathogen of global veterinary significance and a challenging candidate for reliable vaccination. Veterinary Microbiology, 122: 1-15.
  4. Alsaid, M., Mohd Daud, H.H., Mustapha, N.M., Bejo, S.K., Abdelhadi, Y.M., Abuseliana, A.F., Hamdan, R.H. (2013). Pathological findings of experimental Streptococcus agalactiae infection in red hyprid tilapia (Oreochromis sp.). International Conference on Chemical, Agricultural and Medical Sciences. December 29-30, Kuala Lumpur, Malaysia. pp. 70-73.
  5. Amal, M.N.A., Zamri-Saad, M. (2011). Streptococcosis in Tilapia (Oreochromis niloticus): A Review. Pertanika Journal of Tropical Agricultural Science, 34 (2): 195 - 206.
  6. Badr, M.O.T., Hashem, M.A., Elmandrawi, S.A. (2012). Clinicopathological studies on some antibiotics used in Nile tilapia infected with Streptoccocus iniae. Journal of American Science, 8(12): 1057-1070. 
  7. Bromage, E. S., Owens, L. (2002). Infection of barramundi Lates calcarifer with Streptococcus iniae: effects of different routes of exposure. Diseases of Aquatic Organisms, 52(3): 199-205. DOI: 10.3354/dao052199. 
  8. Baums, C.G., Hermeyer, K., Leimbach, S., Adamek, V., Czerny, C.P., Horstgen-Schwark, G., et al (2013). Establishment of a Model of Streptococcus iniae Meningoencephalitis in Nile Tilapia (Oreochromis niloticus). Journal of Comparative Pathology, 149: 94-102.
  9. Bowser, P.R., Wooster, G.A., Getchell, R.G., Timmons, M.B. (1998). Streptococcus iniae infection of Tilapia Oreochromis niloticus in a recirculation production facility. Journal of The World Aquaculture Society. 29(3): 335-339. 
  10. Bromage, E. (2004). The humoral immune response of Lates calcarifer to Streptococcus iniae. Doctorate of Philosophy thesis, James Cook University, Australia. 
  11. Chen, C.Y., Wooster, G.A., Bowser, P.R. (2004). Comparative blood chemistry and histopathology of tilapia infected with Vibrio vulnificus or Streptococcus iniae or exposed to carbon tetrachloride, gentamicin, or copper sulfate. Aquaculture, 239: 421-443.
  12. Chen, C.Y., Bowser, P.R., Chao, C.B. (2007). Comparative histopathology of Streptococcus iniae and Streptococcus agalactiae infected tilapia. Bulletin of European Association Fish Pathologists, 27(1): 2-9.
  13. Creeper, J.H., Buller, N.B. (2006). An outbreak of Streptococcus iniae in barramundi (Lates calcarifera) in freshwater cage culture. Australian Veterinary Journal, 84(11): 408-411.
  14. Çaðýrgan H., (2004). Biotyping of Lactococcus garvieae isolated from Turkey. Ege Üniversitesi Su Ürünleri Dergisi, 21(3-4): 267-269. 
  15. Diler, Ö., Altun, S., Adiloðlu, A.K., Kubilay, A., Iþýklý, B., (2002). First occurence of Streptococcosis affecting farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Bulletin of the European Association of Fish Pathologists, 22(1): 21-26. 
  16. Dodson, S. V., Maurer J, J, , Shotts, E. B. (1999). Biochemical and molecular typing of Streptococcus iniae isolated from fish and human cases. Journal of Fish Diseases, 22: 331-336.
  17. Evans, J.J., Shoemaker, C.A., Klesius, P.H. (2000). Experimental Streptococcus iniae infection of hybrid striped bass Morone chrysops x Morone saxatilis and tilapia Oreochromis niloticus by nares inoculation. Aquaculture, 189: 197–210. 
  18. Fawzy, N.M., Osman, K.M., Sayed Ibrahim, M.E.D.E., Mohamed Ali, M.N., Elrahman, S.S.E. (2014). Streptococcosis in tilapia: Clinico-pathological picture of experimentally infected tilapia. Life Science Journal, 11(9): 1005-1012.
  19. Gürgün, V., Halkman, A.K. (1990). Mikrobiyolojide Sayým Yöntemleri. Gýda Teknolojisi Derneði, Yayýn No 7, Ankara, Türkiye
  20. Hossain, M., Ehsan, A., Rahman, A., Haq, M., Chowdhury, B.R. (2014). Transmission and pathology of Streptococcus inane in monosex Nile tilapia (Oreochromis niloticus) in aquaculture of Bangladesh. Journal of Fisheries, 2(1): 90-99.
  21. Keirstead, N. D., Brake, J. W., Griffin, M. J., Halliday-Simmonds, I., Thrall, M. A., Soto, E. (2014). Fatal septicemia caused by the Zoonotic bacterium streptococcus iniae during an Outbreak in Caribbean Reef Fish. Veterinary Pathology, 51(5): 1035-1041.
  22. Nguyen, H.T., Kanai, K., Yoshikoshi, K. (2001). Immunohistochemical Examination of Experimental Streptococcus iniae Infection in Japanese Flounder Paralichthys olivaceus. Fish Pathology, 36(3): 169-178. 
  23. Oda, S.S., Tohamy, H.G., Massoud, R.G. (2016). Pathological alterations in Nile tilapia experimentally infected with Streptococcus iniae and Candida albicans. Turkish Journal of Fisheries and Aquatic Sciences, 16: 779-788.
  24. Özer, S., Bulduklu, P.S., Dönmez, E. (2008). Mersin Ýlinde Yetiþtiriciliði Yapýlan Gökkuþaðý Alabalýklarýnda (Oncorhynchus mykiss, Walbaum) Streptokokkozis Varlýðý. Journal of Fisheries Sciences.com. 2(3): 272-283. 
  25. Pier, G.B., Madin, S.H. (1976). Streptococcus iniae sp. nov., a beta hemolytic Streptococcus isolated from an Amazon freshwater dolphin, Inia geoffrensis. International Journal of Systematic Bacteriology, 26: 545–553.
  26. Pretto-Giordano, L.G., Müller, E.E., Klesius, P., Gomes da Silva, V. (2010). Efficacy of an experimentally inactivated Streptococcus agalactiae vaccine in Nile tilapia (Oreochromis niloticus) reared in Brazil. Aquaculture Research, 41: 1539-1544.
  27. Perera, R.P., Fiske, R.A., Johnson, S.K. (1998). Histopathology of hybrid tilapias ýnfected with a biotype of Streptococcus iniae. Journal of Aquatic Animal Health, 10: 294 - 299
  28. Rahmatullah, M., Ariff, M., Kahieshesfandiari, M, Daud, H.M., Zamri-Saad, M., Sabri, M.Y., Amal, M.N.A., Ina-Salwany, M.Y. (2017). Isolation and Pathogenicity of Streptococcus iniae in cultured red hybrid tilapia in Malaysia. Journal of Aquatic Animal Health. 29(4): 208-213. DOI: 10.1080/08997659.2017.1360411.
  29. Raissy, M., Zandi, S., Ahmadi, A., Foroutan, M.S., Fadaeifard, F. (2012). Experimental evaluation of pathogenicity of Streptococcus iniae in Silver Shark and Rainbow Shark. African Journal of Microbiology Research, 6(14): 3560-3563.
  30. Rattanachaikunsopon, P., Phumkhachorn, P. (2009). Protective effect of clove oil-supplemented fish diets on experimental Lactococcus garvieae infection in tilapia. Bioscience Biotechnology and Biochemistry, 73(9): 2085-9.
  31. Rodkhum, C., Kayansamruaj, P., Pirarat, N., Wongtawatchai, J. (2012). Duplex PCR for simultaneous and unambiguous detection of Streptococcus iniae and Streptococcus agalactiae associated with streptococcosis of cultured tilapia in Thailand. The Thai Journal of Veterinary Medicine, 42(2): 153-158.
  32. Russo, R., Mitchell, H., Yanong, R.P.E. (2006). Characterization of Streptococcus iniae isolated from ornamental cyprinid fishes and development of challenge models. Aquaculture, 256: 105-110. DOI:10.1016/j.aquaculture.2006.02.046.
  33. Shoemaker, C.A, Evans, J.J., Klesius, P.H. (2000). Density and dose: factors affecting mortality of Streptococcus iniae infected tilapia (Oreochromis niloticus). Aquaculture 188: 229 - 235.
  34. Suanyuk, N., Sukkasame, N., Tanmark, N., Yoshida, T., Itami, T., Thune, R.L., Tantikitti, C., Supamattaya, K. (2010). Streptococcus iniae infection in cultured Asian sea bass (Lates calcarifer) and red tilapia (Oreochromis sp.) in southern Thailand. Songklanakarin Journal of Science and Technology, 32(4): 341-348. 
  35. Sun, J.R., Yan, J.C, Yeh, C.Y., Lee, S.Y., Lu, J.J. (2007). Invasive infection with Streptococcus iniae in Taiwan. Journal of Medical Microbiology, 56: 1246-1249. DOI 10.1099/jmm.0.47180-0.
  36. Zhu, J., Li, C., Ao, Q., Tan, Y., Luo, Y., Guo, Y., Lan, G., Jiang, H., Gan, X. (2015). Trancriptomic profiling revealed the signatures of acute immune response in tilapia (Oreochromis niloticus) following Streptococcus iniae challenge. Fish and Shellfish Immunology, 46: 346-353. DOI: 10.1016/j.fsi.2015.06.027. 

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