Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Research Article

Indian Journal of Animal Research, volume 53 issue 6 (june 2019) : 724-730

Effect of TRIM28 on proliferation, apoptosis and histone H3K9 trimethylation in bovine fibroblasts

Panpan Ma, Xiaxia Man, Shubao Yang, Xiaoqing Dong, Liyan Su, Weimin Luan, Xin Ma
1College of Animal Science and Technology, Jilin Agricultural University, Changchun 130 118, China.
Cite article:- Ma Panpan, Man Xiaxia, Yang Shubao, Dong Xiaoqing, Su Liyan, Luan Weimin, Ma Xin (2019). Effect of TRIM28 on proliferation, apoptosis and histone H3K9 trimethylation in bovine fibroblasts. Indian Journal of Animal Research. 53(6): 724-730. doi: 10.18805/ijar.B-883.

ABSTRACT

TRIM28 is a co-repressor, which interacts with HP1 proteins and chromatin repressive complexes leading to gene expression silencing. In this study, after we used Lipofectamine3000-mediating siRNA and restructured p EGFP-IRES2-TRIM28 to transfect bovine fibroblasts, the expression levels of TRIM28, HP1BP3, DNMT, SETDB1, TP53, BAX, Bcl and SOD were detected by real-time polymerase chain reaction (PCR). In addition, the influence of p EGFP-IRES2-TRIM28 on histone H3K9me3 was also observed. In the siRNA transfected group, TRIM28 was down-regulated (P<0.01), which inhibited cell proliferation and reduced the level of H3K9me3 expression. In the p EGFP-IRES2-TRIM28 transfected group, TRIM28 was over-expressed (P<0.01), and the pro-apoptosis gene BAX was significantly decreased (P<0.01), but there was no significant change in other genes. Our results demonstrate that TRIM28 plays an important role in bovine fibroblasts and might be a valuable biomolecule for proliferation, apoptosis, and histone H3K9 trimethylation.

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