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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 54 issue 11 (november 2020) : 1438-1440,   Doi: 10.18805/ijar.B-870

New Approach to Use Blood Smears for Diagnosis of Bovine Theileriosis
 

A
A.A.T. Al-hosary
A
A. Nordengrahn
M
M. Merza
1Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt.
Cite article:- Al-hosary A.A.T., Nordengrahn A., Merza M. (2018). New Approach to Use Blood Smears for Diagnosis of Bovine Theileriosis. Indian Journal of Animal Research. 54(11): 1438-1440. doi: 10.18805/ijar.B-870.

ABSTRACT

The present study held to investigate the relationship between azurophilic granules in the peripheral blood leukocytes and bovine theileriosis. A total number of 100 adult cattle of different sexes were subjected to this study. Whole blood, blood and lymph smears were collected from each animal under study. Theileria annulata was confirmed by both conventional and molecular methods. The obtained results revealed that the percentages of interaerythrocytic stage (Signet Ring), azurophilic granules and Koch’s blue bodies in the samples of the examined cattle were 28%, 42% and 39%, respectively. Evaluation of the reliability of azurophilic granules occurrence in peripheral blood leukocytes as novel method for diagnosis of tropical theileriosis revealed that the occurrence of these granules should be considered as an easy, inexpensive and rapid method for diagnosis of bovine theileriosis.

REFERENCES

  1. AL-Gaabary, M.H. (1995). Epidemiological and immunological studies on bovine tropical theileriosis. PhD, Zagazig University, Egypt.
  2. AL-Hosary, A.A. (2013). Molecular typing of bovine theileriosis in Upper Egypt. PhD Assiut University, Egypt. 
  3. Charles, M. H., 2002 Text Book for Laboratory Procedures for Veterinary Technicians. Elsevier Science.
  4. D’Oliveira, C., Van der weide, M., Habela, M.A., Jacquiet, P., Jongejan, F. (1995). Detection of Theileria annulata in blood samples of carrier cattle by PCR. Journal of Clinical Microbiology, 33:2665–2669.
  5. Dolan, T.T., Morzaria, S.P. (1992). Detection of carrier state in Theileria parva infected cattle by the polymerase chain reaction. Parasitology, 2:215-232. 
  6. Gharbi, M., Latrach, R., Sassi, L., Darchouth, M.A. (2012). Evaluation of simple Theileria annulata protocol from experimental infected bovine whole blood. Parasite 19: 281-283.
  7. International Laboratory for Research on Animal Diseases Published by the International (ILRAD) Reports, (1984). Studies of parasite biology with the electron microscope, 2: 2.
  8. Irvin, A.D., Cunningham, M.P., Young, A.S. 1981 Advances in the Control of Theileriosis. Martinus Nijhoff Publishers.
  9. Jabbar, A.S., Shayan, P., Hugel, F.U., Biermann, R., Ewald, C.H., Schein, E., Gerder, J. (1997). Macroschizonts of Theileria annulata as a vaccine and diagnostic tools. Tropical Animal Health Production, 29:128–132.
  10. Kirvar, E., Ilhan, T., Katzer, F., Hooshmand-Red, P., Zweygarth, E., Gerstenberg, C., Phipps, P., Brown, C.G. (2000). Detection of Theileria annulata in cattle and vector ticks by PCR using the Tams-1 gene sequences. Parasitology, 120:245–254.
  11. Maharana, B.R., Kumar, B., Prasad, A., Patbandha, T.K., Sudhakar, N.R., Joseph, J.P. Patel, B.R. (2016). Prevalence and assessment of risk factors for haemoprotozoan infections in cattle and buffaloes of South-West Gujarat, India. Indian Journal of Animal Research, 50:733-739.
  12. Nagaty, H.F. (1947). Some new and rear records of piroplasmosis with a list of the species Babesia and Theileria recorded from Egypt. Veterinary Record, 59:145. 
  13. Pipano, E., Shkap, V., Frank, M. (1990). Comparison of three methods for initating in vetro culture of Theileria annulata schizont. Revue d’eìlevage et de meìdecine veìteìrinaire des pays tropicaux, 4:529-533.
  14. Sangwan, N., Sangwan, A.K. (2007). Changes in Blood and Tissue Copper levels due to Theileria annulata Infection in Cross-breed calves. Indian Journal of Animal Research, 41: 278-281. 
  15. Shkap, V., Frank, M., Fish, L., Pipano, E. (1995). Theileria annulata in vitro cultivation of schizont – infected lymphocytes. Veterinary Parasitology, 65:11-20.
  16. Tabidi, M.H., Hassan, O.M., El Jalii, I.M., Hamza, A.E. (2006). Surveillance of theileriosis in selected dairy farms in Khartoum and Gaziera states, Sudan. Journal of Animal and Veterinary Advances, 5:1043–1045.
  17. Thrusfield, M. 2005. Veterinary Epidemiology. Blackwell Science publisher. 
  18. Vantansever, Z., Nalbantoglu, S. (2002). Detection of cattle infected with Theileria annulata in fields by nested PCR, IFAT and microscopic examination of blood smears. Turkish Journal of Veterinary and Animal Sciences, 26:1465–1469. 
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    Research Article

    volume 54 issue 11 (november 2020) : 1438-1440,   Doi: 10.18805/ijar.B-870

    New Approach to Use Blood Smears for Diagnosis of Bovine Theileriosis
     

    A
    A.A.T. Al-hosary
    A
    A. Nordengrahn
    M
    M. Merza
    1Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, Assiut University, 71526, Assiut, Egypt.
    Cite article:- Al-hosary A.A.T., Nordengrahn A., Merza M. (2018). New Approach to Use Blood Smears for Diagnosis of Bovine Theileriosis. Indian Journal of Animal Research. 54(11): 1438-1440. doi: 10.18805/ijar.B-870.

    ABSTRACT

    The present study held to investigate the relationship between azurophilic granules in the peripheral blood leukocytes and bovine theileriosis. A total number of 100 adult cattle of different sexes were subjected to this study. Whole blood, blood and lymph smears were collected from each animal under study. Theileria annulata was confirmed by both conventional and molecular methods. The obtained results revealed that the percentages of interaerythrocytic stage (Signet Ring), azurophilic granules and Koch’s blue bodies in the samples of the examined cattle were 28%, 42% and 39%, respectively. Evaluation of the reliability of azurophilic granules occurrence in peripheral blood leukocytes as novel method for diagnosis of tropical theileriosis revealed that the occurrence of these granules should be considered as an easy, inexpensive and rapid method for diagnosis of bovine theileriosis.

    REFERENCES

    1. AL-Gaabary, M.H. (1995). Epidemiological and immunological studies on bovine tropical theileriosis. PhD, Zagazig University, Egypt.
    2. AL-Hosary, A.A. (2013). Molecular typing of bovine theileriosis in Upper Egypt. PhD Assiut University, Egypt. 
    3. Charles, M. H., 2002 Text Book for Laboratory Procedures for Veterinary Technicians. Elsevier Science.
    4. D’Oliveira, C., Van der weide, M., Habela, M.A., Jacquiet, P., Jongejan, F. (1995). Detection of Theileria annulata in blood samples of carrier cattle by PCR. Journal of Clinical Microbiology, 33:2665–2669.
    5. Dolan, T.T., Morzaria, S.P. (1992). Detection of carrier state in Theileria parva infected cattle by the polymerase chain reaction. Parasitology, 2:215-232. 
    6. Gharbi, M., Latrach, R., Sassi, L., Darchouth, M.A. (2012). Evaluation of simple Theileria annulata protocol from experimental infected bovine whole blood. Parasite 19: 281-283.
    7. International Laboratory for Research on Animal Diseases Published by the International (ILRAD) Reports, (1984). Studies of parasite biology with the electron microscope, 2: 2.
    8. Irvin, A.D., Cunningham, M.P., Young, A.S. 1981 Advances in the Control of Theileriosis. Martinus Nijhoff Publishers.
    9. Jabbar, A.S., Shayan, P., Hugel, F.U., Biermann, R., Ewald, C.H., Schein, E., Gerder, J. (1997). Macroschizonts of Theileria annulata as a vaccine and diagnostic tools. Tropical Animal Health Production, 29:128–132.
    10. Kirvar, E., Ilhan, T., Katzer, F., Hooshmand-Red, P., Zweygarth, E., Gerstenberg, C., Phipps, P., Brown, C.G. (2000). Detection of Theileria annulata in cattle and vector ticks by PCR using the Tams-1 gene sequences. Parasitology, 120:245–254.
    11. Maharana, B.R., Kumar, B., Prasad, A., Patbandha, T.K., Sudhakar, N.R., Joseph, J.P. Patel, B.R. (2016). Prevalence and assessment of risk factors for haemoprotozoan infections in cattle and buffaloes of South-West Gujarat, India. Indian Journal of Animal Research, 50:733-739.
    12. Nagaty, H.F. (1947). Some new and rear records of piroplasmosis with a list of the species Babesia and Theileria recorded from Egypt. Veterinary Record, 59:145. 
    13. Pipano, E., Shkap, V., Frank, M. (1990). Comparison of three methods for initating in vetro culture of Theileria annulata schizont. Revue d’eìlevage et de meìdecine veìteìrinaire des pays tropicaux, 4:529-533.
    14. Sangwan, N., Sangwan, A.K. (2007). Changes in Blood and Tissue Copper levels due to Theileria annulata Infection in Cross-breed calves. Indian Journal of Animal Research, 41: 278-281. 
    15. Shkap, V., Frank, M., Fish, L., Pipano, E. (1995). Theileria annulata in vitro cultivation of schizont – infected lymphocytes. Veterinary Parasitology, 65:11-20.
    16. Tabidi, M.H., Hassan, O.M., El Jalii, I.M., Hamza, A.E. (2006). Surveillance of theileriosis in selected dairy farms in Khartoum and Gaziera states, Sudan. Journal of Animal and Veterinary Advances, 5:1043–1045.
    17. Thrusfield, M. 2005. Veterinary Epidemiology. Blackwell Science publisher. 
    18. Vantansever, Z., Nalbantoglu, S. (2002). Detection of cattle infected with Theileria annulata in fields by nested PCR, IFAT and microscopic examination of blood smears. Turkish Journal of Veterinary and Animal Sciences, 26:1465–1469. 
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