Effects of oral administration of various essential oils on blood metabolites, intestine development, microbial enumeration and meat quality in broilers

DOI: 10.18805/ijar.B-836    | Article Id: B-836 | Page : 762-767
Citation :- Effects of oral administration of various essential oils on blood metabolites, intestine development, microbial enumeration and meat quality in broilers.Indian Journal Of Animal Research.2019.(53):762-767
S.D. Liu, M.H. Song, W. Yun, J.H. Lee, C.H. Lee, W.G. Kwak, H.J. Oh, H.B. Kim and J.H. Cho
liushudong818@163.com
Address :
Division of Food and Animal Science, Chungbuk National University, Cheongju-si-361 763, Republic of Korea.
Submitted Date : 25-09-2017
Accepted Date : 7-09-2018

Abstract

A total of eighty 21-day-olds (1.1±0.01kg) ROSS 308 broilers were randomly allocated into 4 groups, with 20 replicates per group and 1 chicken per replicate per cage. Three experimental groups (CAR, THY, ORE group) were given oral administration of 200ìL essential oils (carvacrol, thyme and oregano) at 18:00h every day. The results showed no significant differences on blood metabolites among each group (P>0.05). The villus height of the small intestines in the CAR group increased significantly (P<0.05) from 1360.10 to 1552.54ìm as compared to the control group, the goblet cell counts in the THY and ORE groups significantly increased by 20.91% (P<0.05) and 31.82% (P<0.05), respectively, compared to the control group. The E.coli and Salmonella in ORE group significantly decreased by 11.16% (P<0.05) and 9.54% (P<0.05), respectively, compared to the control group. The Redness (a*) values of the muscles of the CAR and ORE groups were observed to increase significantly (P<0.05) from 3.58 to 7.95 and 6.86, respectively, compared to the control group. In conclusion, oral administration of the essential oils carvacrol, thyme, and oregano promoted intestinal development, improved the intestinal environment and the meat quality of broilers.

Keywords

Broilers Essential Oils Meat Quality Oral Administration .

References

  1. Aksu, T. and Bozkurt, A. S. (2009). Effect of dietary essential oils and/or humic acids on broiler performance, microbial population of intestinal content and antibody titres in the summer season. Kafkas Üniversitesi Veteriner Fakültesi Dergisi.15: 185-190. DOI:10.9775/kvfd.2008.89-A
  2. Alp, M.; Midilli, M.; Kocabaðlý, N.; Yýlmaz, Turan N. and Gargýlý, A. (2012). The effects of dietary oregano essential oil on live performance, carcass yield, serum immunoglobulin G level, and oocyst count in broilers. Journal of Applied Poultry Research. 21:630-636. DOI: https://doi.org/10.3382/japr.2012-00551
  3. Bi SF, Zhu G Q, Wu J, Li ZK, Lv YZ, and Fang L. Chemical composition and antioxidant activities of the essential oil from nandina domestica fruits. Natural Product Research 2016; 30(3), 362-5. DOI: 10.1080/14786419.2015.1057584
  4. Bhaisare, D. B., Thyagarajan, D., Churchil, R. R., and Punniamurthy, N. (2016). In-vitro antimicrobial efficacy of certian herbal seeds essential oils against important poultry microbes. Indian Journal of Animal Research(OF). DOI: 10.18805/ijar.7089
  5. Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods—A review. International Journal of Food Microbiology, 94: 223-253. DOI: 10.1016/j.ijfoodmicro.2004.03.022
  6. Chao L K, Hua KF, Hsu H Y., Cheng SS, Lin IF, Chen CJ and Chang ST. (2008). Cinnamaldehyde inhibits pro-inflammatory cytokines secretion from monocytes / macrophages through suppression of intracellular signaling. Food and Chemical Toxicology. 46: 220-231. DOI: 10.1016/j.fct.2007.07.016
  7. Clevers, H. (2013). The intestinal crypt, a prototype stem cell compartment. Cell, 154: 274-284. DOI: https://doi.org/10.1016/    j.cell.2013.07.004
  8. Du E, Wang W, Gan L, Li Z, Guo S, and Guo Y. (2016). Effects of thymol and carvacrol supplementation on intestinal integrity and immune responses of broiler chickens challenged with Clostridium perfringens. Journal of Animal Science and Biotechnology. 7: 19-29. DOI 10.1186/s40104-016-0079-7
  9. Faleiro ML, Miguel MG, Ladeiro F, Venâncio F, Tavares R, and Brito JC. (2003) Antimicrobial activity of essential oils isolated from portuguese endemic species of thymus. Letters in Applied Microbiology; 36, 35-40. DOI: https: //doi.org/ 10.1046/ j.1472-    765X.2003.01259.x
  10. Farhad S, Armin T, Saeed Z, Amir KT., Zarbakht AP, and Pedram,G. (2013). Effect of dietary addition of thymus vulgaris, mentha piperita, cirtus lemon and carum copticum essential oils on beast meat quality of male broilers. Research on Animal Production. 4: 78-91.
  11. Jang IS, KO YH, Kang SY, and Lee CY. (2007). Effect of a commercial essential oil on growth performance, digestive enzyme activity and intestinal microflora population in broiler chickens. Animal Feed Science and Technology. 134: 304-315.
  12. Kaya, H., Kaya, A., Celebi, S., and MaciT, M. (2013). Effects of dietary supplementation of essential oils and vitamin e on performance, egg quality and escherichia coli count in excreta. Indian Journal of Animal Research, 47: 515-520.
  13. Kauffman RG, Eikelenboom G, Wal P G V D, Engel B, and ZAAR M. A comparison of methods to estimate water-holding capacity in post-rigor porcine muscle. Meat Science 1986; 18(4), 307-22. DOI: 10.1016/0309-1740(86)90020-3.
  14. Kim, Y. S., and Ho, S. B. (2010). Intestinal goblet cells and mucins in health and disease: recent insights and progress. Current Gastroenterology Reports, 12: 319-330. DOI: 10.1007/s11894-010-0131-2.
  15. Kirkpinar, F., and Turp, G. Y. (2014). Effects of dietary oregano and garlic essential oils on carcass characteristics, meat composition, colour, ph and sensory quality of broiler meat. Br Poult Sci, 55: 157-166. DOI: 10.1080/00071668.2013.879980.
  16. Mehr, M. A., Hassanabadi, A., Moghaddam, H. N., & Kermanshahi, H. (2014). Supplementation of clove essential oils and probiotic on blood components, lymphoid organs and immune response in broiler chickens. Research Opinions in Animal & Veterinary Sciences, 4: 218-223. 
  17. National Research Council. 1994. Nutrient Requirements of Poultry, 9th rev. ed. National Academy Press. Washington, D
  18. Najafi P and Torki M. (2010). Performance, blood metabolites and immunocompetence of broiler chicks fed diets included essential oils of medicinal herbs. Journal of Animal and Veterinary Advances. 9: 1164-1168. DOI: 10.3923/javaa.2010.1164.1168.
  19. Parvar R, Khosravinia H, and Azarfar A. (2013).Supplementation effect of Satureja essential oils in drinking water on immune conduct of broiler chickens reared under stress of heat. Journal of Cell and Animal Biology. 7:121-124. DOI: 10.5897/JCAB2013.0392
  20. Qiao M, Fletcher DL, Smith DP, and Northcutt JK. (2001). The effect of broiler breast meat color on pH, moisture, water-holding capacity, and emulsification capacity. Poultry Science. 80: 676-80. DOI: https://doi.org/10.1093/ps/80.5.676.
  21. Reisinger, N., Steiner, T., Nitsch, S., Schatzmayr, G., and Applegate, T. J. (2011). Effects of a blend of essential oils on broiler performance and intestinal morphology during coccidial vaccine exposure. Journal of Applied Poultry Research, 20: 272-    283. DOI: 10.3382/japr.2010-00226
  22. SPSS. 2012. SPSS for Windows, release 22.0, SPSS Inc., Chicago, IL, USA
  23. Shao Q, Deng Y, Liu H, Zhang A, Huang Y, Xu G and Li M.(2014) Essential oils extraction from Anoectochilus roxburghii using supercritical carbon dioxide and their antioxidant activity. Industrial Crops and Products. 60: 104-112. https://doi.org/    10.1016/j.indcrop.2014.06.009.
  24. Suhail N, Bilal N, and Hasan S. (2015). Chronic unpredictable stress (CUS) enhances the carcinogenic potential of 7,12-dimethylbenz(a) anthracene (DMBA) and accelerates the onset of tumor development in Swiss albino mice. Cell Stress and Chaperones. 20:1023-1036. DOI: 10.1007/s12192-015-0632-x.
  25. Wallace. John, R., (2004). Antimicrobial properties of plant secondary metabolites. Proc Nutr Soc, 63: 621-629. DOI: 10.1079/    PNS2004393
  26. Yamauchi K, Buwjoom T, Koge K, and Ebashi T. (2006). Histological alterations of the intestinal villi and epithelial cells in chickens fed dietary sugar cane extract. British Poultry Science.47: 544-553.DOI: https://doi.org/10.1080/00071660600963149.
  27. Yoon WJ, Kim SS, Oh TH, Lee NH, and Hyun CG. (2009). Cryptomeria japonica essential oil inhibits the growth of drug-resistant skin pathogens and LPS-induced nitric oxide and pro-inflammatory cytokine production. Polish Journal of Microbiology.58: 61-68.
  28. Yoon WJ, Moon JY, Song G, Lee YK, Han MS, Lee JS, and Hyun CG. (2010). Artemisia fukudo essential oil attenuates LPS-induced inflammation by suppressing NF-êB and MAPK activation in RAW 264.7 macrophages. Food and Chemical Toxicology .48: 1222-1229. 
  29. Zarei, A., Babainia, M., and Noroozian, H. (2013). Effect of a commercial feed additive (biacid®) on performance, carcass traits and blood parameters of broilers. Indian Journal of Animal Research, 47: 244-246. 

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