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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 4 (april 2019) : 451-456,   Doi: 10.18805/ijar.B-822

Icariin impacts molecular changes in estrogen receptors but not other reproductive parameters in female mice

C
Caroline W. Muneri
S
Seth Y. Afedo
Y
Yaping Xu
G
Guochao Ni
Q
Qingxia Shen
R
Rong Rui
1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu-210 095, China
Cite article:- Muneri W. Caroline, Afedo Y. Seth, Xu Yaping, Ni Guochao, Shen Qingxia, Rui Rong (2018). Icariin impacts molecular changes in estrogen receptors but not other reproductive parameters in female mice. Indian Journal of Animal Research. 53(4): 451-456. doi: 10.18805/ijar.B-822.

ABSTRACT

This study was conducted to assess the effects of icariin (ICA) an ingredient of Epimedium species, on reproductive parameters in mice. Serum, uterus and ovarian samples were collected after treatment. We determined the reproductive index (RI) and in vitro maturation (IVM) rates of oocytes. The serum was tested for 17b-estradiol (17b-E2) and progesterone (P4) using radioimmunoassay (RIA) and ovaries were analyzed for mRNA expression of Steroidogenic acute regulatory protein (Star), Cytochrome p450 family 19 subfamilies a polypeptide 1 (Cyp19a1) and 17b-hydroxysteroid dehydrogenase (Hsd17b1), genes using Real-time Quantitative RT-PCR (RT-qPCR). Protein expression of estrogen receptors (ER) a, b, and progesterone receptor A (PRA) were detected using western blot (WB). ERa from treatment group was increased (p<0.05) while ERb and PRA were decreased (p<0.05). ICA regulated ovarian development through the estrogen receptor pathway. ERa expression was evidently increased and this may be the pathway that is utilized for its mode of action.

REFERENCES

  1. Afedo, S.Y., Xu, Y., Muneri, C.W., Ni, G., Xu, W., and Rui, R. (2015). Effects of Epimedium polysaccharide on female mouse (Mus musculus) ovarian and uterine development. Turkish Journal of Veterinary and Animal Science, 39: 714-718. 
  2. Beck, V., Rohr, U., and Jungbauer, A., (2005). Phytoestrogens derived from red clover: an alternative to estrogen replacement therapy? Journal of Steroid Biochemistry and Molecular Biology, 94: 499-518.
  3. Behringer, R. R., Gertsenstein, M., Nagy, K. V., and Nagy, A., (2014). Manipulating the mouse embryo: A laboratory manual. 4th ed. Cold Spring Harbor Laboratory Press. pp 140-170
  4. De Naeyer, A., Pocock, V., Milligan, S., and De Keukeleire, D. (2005). Estrogenic activity of a polyphenolic extract of the leaves of Epimedium brevicornum. Fitoterapia,76: 35-40.
  5. Dhami, A.J., Theodore, V.K. , Panchal ,M.T., Hadiya, K.K., Lunagariya, P.M. and Sarvaiya, N.P. (2017) Effect of peripartum nutritional supplementation on postpartum fertility and blood biochemical and steroid hormone profile in crossbred cows. Indian Journal Of Animal Research 51 (5) 821-826
  6. Fisher, C., Graves, K., Parlow, A., Simpson E., (1998) Characterization of mice deficient in aromatase (ArKO) because of targeted disruption of the cyp19 gene Proceedings of the National. Academy of Sciences 95 (12) 6965-6970
  7. Hewitt, S. C., and Korach, K. S., (2000) Progesterone action and responses in the aERKO mouse. Steroids 65 (10) 551-555
  8. Hewitt, S. C., and Korach, K. S. (2003). Oestrogen receptor knockout mice: roles for oestrogen receptors alpha and beta in reproductive tissues. Reproduction, 125: 143-149.
  9. Ju, S., Rui, R., Lu, Q., Lin, P., and Guo, H. (2010). Analysis of apoptosis and methyltransferase mRNA expression in porcine cloned embryos cultured in vitro. Journal of Assisted Reproduction and Genetics, 27: 49-59.
  10. Kamel, H.H., (2013). Role of phyto-oestrogens in ovulation induction in women with polycystic ovarian syndrome. European Journal of Obstetrics, Gynecology and Reproductive Biology, 168: 60-63.
  11. Kang, H. K., Choi, Y. H., Kwon, H., Lee, S. B., Kim D. H., Sung, C. K., Park, Y. I., and Dong, M. S. (2012). Estrogenic/antiestrogenic activities of a Epimedium koreanum extract and its major components: in vitro and in vivo studies. Food and Chemical Toxicology, 50: 2751-2759.
  12. Kang, H. K., Lee SB, Kwon H, Sung CK, Park YI, and Dong MS. (2012). Peripubertal administration of icariin and icaritin advances pubertal development in female rats. Biomolecules and Therapy. (Seoul)., 20: 189-195
  13. Kawao, N., Tamura, Y., Okumoto, K., Yano, M., Okada, K., Matsuo, O., Kaji, H., (2014) Tissue-type plasminogen activator deficiency delays bone repair: roles of osteoblastic proliferation and vascular endothelial growth factor. American Journal of Physiology-    Endocrinology and Metabolism, 307(3): E278-E288.
  14. Kipp, J. L., Kilen, S. M., Woodruff T. K., and Mayo K. E. (2007). Activin regulates estrogen receptor gene expression in the mouse ovary. Journal of Biology and Chemicals, 282: 36755-36765.
  15. Livak, K. J., and Schmittgen, T. D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 25: 402-408.
  16. Matthews, J., and Gustafsson, J. A. (2003) Estrogen signaling: a subtle balance between ER alpha and ER beta. Molecular Interventions, 3: 281-292.
  17. Miller, W. L. and Auchus, R. J. (2011) The molecular biology, biochemistry, and physiology of human steroidogenesis and its disorders. Endocrine review 32 (1) 81-151
  18. Naikoo, M., Dhami A.J. and Ramakrishnan A. (2016) Effect of estrus synchronization on plasma progesterone profile and fertility response in postpartum suckled anestrous Kankrej cows. Indian Journal of Animal Research, 50 (4) 460-465
  19. Novensa, L., Novella, S., Medina, P., Segarra, G., Castillo, N., Heras, M., Hermenegildo, C., Dantas, A. P., (2011) Aging negatively affects estrogens-mediated effects on nitric oxide bioavailability by shifting ERa/ERb balance in female mice. PLoS One, 6(9): e25335.
  20. Ruiz, A., Rockfield, S., Taran, N., Haller, E., Engelman,R. W., Flores, I., Panina-Bordignon, P., Nanjundan, M., (2016) Effect of hydroxychloroquine and characterization of autophagy in a mouse model of endometriosis. Cell death & disease, 7(1): e2059
  21. Shen, P., Guo, B. L., Gong, Y., Hong, D. Y., Hong, Y., and Yong, E. L. (2007) Taxonomic, genetic, chemical and estrogenic characteristics of Epimedium species. Phytochemistry, 68: 1448-1458.
  22. Xue. L., Wang. Y., Jiang. Y., Han. T., Nie. Y., Zhao. L., Zhang. Q., and Qin. L. (2012). Comparative effects of er-xian decoction, epimedium herbs, and icariin with estrogen on bone and reproductive tissue in ovariectomized rats. Evidence Based Complementary Alternative Medicine, 241416.
  23. Yang. L., Lu. D., Guo. J., Meng. X., Zhang. G., and Wang. F. (2013) Icariin from Epimedium brevicornum Maxim promotes the biosynthesis of estrogen by aromatase (CYP19). Journal of Ethnopharmacology, 145:715-721.
  24. Yap. S. P., Shen. P., Li. J., Lee. L. S., and Yong. E. L. (2007) Molecular and pharmacodynamic properties of estrogenic extracts from the traditional Chinese medicinal herb, Epimedium. Journal of Ethnopharmacology, 113: 218-224.
  25. Ye. H., and Lou. Y. (2005) Estrogenic effects of two derivatives of icariin on human breast cancer MCF-7 cells. Phytomedicine, 12:735-741.
  26. Zhang, L., Shi, W., Song, L., Zhang, X., Cheng, L., Wang, Y., Ge, X., Li, W., Zhang, W., et al., (2014) A recurrent deletion mutation in OPA1 causes autosomal dominant optic atrophy in a Chinese family. Scientific reports, 4 6936
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 4 (april 2019) : 451-456,   Doi: 10.18805/ijar.B-822

    Icariin impacts molecular changes in estrogen receptors but not other reproductive parameters in female mice

    C
    Caroline W. Muneri
    S
    Seth Y. Afedo
    Y
    Yaping Xu
    G
    Guochao Ni
    Q
    Qingxia Shen
    R
    Rong Rui
    1College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, Jiangsu-210 095, China
    Cite article:- Muneri W. Caroline, Afedo Y. Seth, Xu Yaping, Ni Guochao, Shen Qingxia, Rui Rong (2018). Icariin impacts molecular changes in estrogen receptors but not other reproductive parameters in female mice. Indian Journal of Animal Research. 53(4): 451-456. doi: 10.18805/ijar.B-822.

    ABSTRACT

    This study was conducted to assess the effects of icariin (ICA) an ingredient of Epimedium species, on reproductive parameters in mice. Serum, uterus and ovarian samples were collected after treatment. We determined the reproductive index (RI) and in vitro maturation (IVM) rates of oocytes. The serum was tested for 17b-estradiol (17b-E2) and progesterone (P4) using radioimmunoassay (RIA) and ovaries were analyzed for mRNA expression of Steroidogenic acute regulatory protein (Star), Cytochrome p450 family 19 subfamilies a polypeptide 1 (Cyp19a1) and 17b-hydroxysteroid dehydrogenase (Hsd17b1), genes using Real-time Quantitative RT-PCR (RT-qPCR). Protein expression of estrogen receptors (ER) a, b, and progesterone receptor A (PRA) were detected using western blot (WB). ERa from treatment group was increased (p<0.05) while ERb and PRA were decreased (p<0.05). ICA regulated ovarian development through the estrogen receptor pathway. ERa expression was evidently increased and this may be the pathway that is utilized for its mode of action.

    REFERENCES

    1. Afedo, S.Y., Xu, Y., Muneri, C.W., Ni, G., Xu, W., and Rui, R. (2015). Effects of Epimedium polysaccharide on female mouse (Mus musculus) ovarian and uterine development. Turkish Journal of Veterinary and Animal Science, 39: 714-718. 
    2. Beck, V., Rohr, U., and Jungbauer, A., (2005). Phytoestrogens derived from red clover: an alternative to estrogen replacement therapy? Journal of Steroid Biochemistry and Molecular Biology, 94: 499-518.
    3. Behringer, R. R., Gertsenstein, M., Nagy, K. V., and Nagy, A., (2014). Manipulating the mouse embryo: A laboratory manual. 4th ed. Cold Spring Harbor Laboratory Press. pp 140-170
    4. De Naeyer, A., Pocock, V., Milligan, S., and De Keukeleire, D. (2005). Estrogenic activity of a polyphenolic extract of the leaves of Epimedium brevicornum. Fitoterapia,76: 35-40.
    5. Dhami, A.J., Theodore, V.K. , Panchal ,M.T., Hadiya, K.K., Lunagariya, P.M. and Sarvaiya, N.P. (2017) Effect of peripartum nutritional supplementation on postpartum fertility and blood biochemical and steroid hormone profile in crossbred cows. Indian Journal Of Animal Research 51 (5) 821-826
    6. Fisher, C., Graves, K., Parlow, A., Simpson E., (1998) Characterization of mice deficient in aromatase (ArKO) because of targeted disruption of the cyp19 gene Proceedings of the National. Academy of Sciences 95 (12) 6965-6970
    7. Hewitt, S. C., and Korach, K. S., (2000) Progesterone action and responses in the aERKO mouse. Steroids 65 (10) 551-555
    8. Hewitt, S. C., and Korach, K. S. (2003). Oestrogen receptor knockout mice: roles for oestrogen receptors alpha and beta in reproductive tissues. Reproduction, 125: 143-149.
    9. Ju, S., Rui, R., Lu, Q., Lin, P., and Guo, H. (2010). Analysis of apoptosis and methyltransferase mRNA expression in porcine cloned embryos cultured in vitro. Journal of Assisted Reproduction and Genetics, 27: 49-59.
    10. Kamel, H.H., (2013). Role of phyto-oestrogens in ovulation induction in women with polycystic ovarian syndrome. European Journal of Obstetrics, Gynecology and Reproductive Biology, 168: 60-63.
    11. Kang, H. K., Choi, Y. H., Kwon, H., Lee, S. B., Kim D. H., Sung, C. K., Park, Y. I., and Dong, M. S. (2012). Estrogenic/antiestrogenic activities of a Epimedium koreanum extract and its major components: in vitro and in vivo studies. Food and Chemical Toxicology, 50: 2751-2759.
    12. Kang, H. K., Lee SB, Kwon H, Sung CK, Park YI, and Dong MS. (2012). Peripubertal administration of icariin and icaritin advances pubertal development in female rats. Biomolecules and Therapy. (Seoul)., 20: 189-195
    13. Kawao, N., Tamura, Y., Okumoto, K., Yano, M., Okada, K., Matsuo, O., Kaji, H., (2014) Tissue-type plasminogen activator deficiency delays bone repair: roles of osteoblastic proliferation and vascular endothelial growth factor. American Journal of Physiology-    Endocrinology and Metabolism, 307(3): E278-E288.
    14. Kipp, J. L., Kilen, S. M., Woodruff T. K., and Mayo K. E. (2007). Activin regulates estrogen receptor gene expression in the mouse ovary. Journal of Biology and Chemicals, 282: 36755-36765.
    15. Livak, K. J., and Schmittgen, T. D. (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 25: 402-408.
    16. Matthews, J., and Gustafsson, J. A. (2003) Estrogen signaling: a subtle balance between ER alpha and ER beta. Molecular Interventions, 3: 281-292.
    17. Miller, W. L. and Auchus, R. J. (2011) The molecular biology, biochemistry, and physiology of human steroidogenesis and its disorders. Endocrine review 32 (1) 81-151
    18. Naikoo, M., Dhami A.J. and Ramakrishnan A. (2016) Effect of estrus synchronization on plasma progesterone profile and fertility response in postpartum suckled anestrous Kankrej cows. Indian Journal of Animal Research, 50 (4) 460-465
    19. Novensa, L., Novella, S., Medina, P., Segarra, G., Castillo, N., Heras, M., Hermenegildo, C., Dantas, A. P., (2011) Aging negatively affects estrogens-mediated effects on nitric oxide bioavailability by shifting ERa/ERb balance in female mice. PLoS One, 6(9): e25335.
    20. Ruiz, A., Rockfield, S., Taran, N., Haller, E., Engelman,R. W., Flores, I., Panina-Bordignon, P., Nanjundan, M., (2016) Effect of hydroxychloroquine and characterization of autophagy in a mouse model of endometriosis. Cell death & disease, 7(1): e2059
    21. Shen, P., Guo, B. L., Gong, Y., Hong, D. Y., Hong, Y., and Yong, E. L. (2007) Taxonomic, genetic, chemical and estrogenic characteristics of Epimedium species. Phytochemistry, 68: 1448-1458.
    22. Xue. L., Wang. Y., Jiang. Y., Han. T., Nie. Y., Zhao. L., Zhang. Q., and Qin. L. (2012). Comparative effects of er-xian decoction, epimedium herbs, and icariin with estrogen on bone and reproductive tissue in ovariectomized rats. Evidence Based Complementary Alternative Medicine, 241416.
    23. Yang. L., Lu. D., Guo. J., Meng. X., Zhang. G., and Wang. F. (2013) Icariin from Epimedium brevicornum Maxim promotes the biosynthesis of estrogen by aromatase (CYP19). Journal of Ethnopharmacology, 145:715-721.
    24. Yap. S. P., Shen. P., Li. J., Lee. L. S., and Yong. E. L. (2007) Molecular and pharmacodynamic properties of estrogenic extracts from the traditional Chinese medicinal herb, Epimedium. Journal of Ethnopharmacology, 113: 218-224.
    25. Ye. H., and Lou. Y. (2005) Estrogenic effects of two derivatives of icariin on human breast cancer MCF-7 cells. Phytomedicine, 12:735-741.
    26. Zhang, L., Shi, W., Song, L., Zhang, X., Cheng, L., Wang, Y., Ge, X., Li, W., Zhang, W., et al., (2014) A recurrent deletion mutation in OPA1 causes autosomal dominant optic atrophy in a Chinese family. Scientific reports, 4 6936
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