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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 12 (december 2018) : 1705-1710,   Doi: 10.18805/ijar.B-807

Parity-dependent association between IL-12Râ2 and IL-23R gene polymorphisms and clinical mastitis in Chinese Holstein cows

M
Mengqi Wang
W
Wei Ni
H
Huimin Zhang
X
Xiaolong Wang
Z
Zhangping Yang
E
Eveline M. Ibeagha-Awemu
Y
Yongjiang Mao
1College of Animal Science and Technology, Yangzhou University, 225 009, Yangzhou, P. R. China.
Cite article:- Wang Mengqi, Ni Wei, Zhang Huimin, Wang Xiaolong, Yang Zhangping, Ibeagha-Awemu M. Eveline, Mao Yongjiang (2018). Parity-dependent association between IL-12Râ2 and IL-23R gene polymorphisms and clinical mastitis in Chinese Holstein cows. Indian Journal of Animal Research. 52(12): 1705-1710. doi: 10.18805/ijar.B-807.

ABSTRACT

Interleukin-12 (IL-12) and interleukin-23 (IL-23) are proinflammatory cytokines produced by macrophages and dendritic cells in response to infection with intracellular pathogens. Given the importance of IL-12 and IL-23 for modulating inflammation and the host immune response, the IL-12 and IL-23 receptor genes may be suitable candidate genes for studying disease resistance in dairy cattle. DNA samples of twenty Chinese Holstein cows were selected randomly for PCR amplification and sequencing, and used for SNP discovery in the bovine IL-12Râ2 and IL-23R promoter regions. One SNP (c.-246G>T, rs385343195) in IL-12Râ2 and 2 SNPs [c.-856A>G (rs109216732) and c.-207T>C (rs109571825)] in IL-23R were identified. Identified SNPs were genotyped in 866 Chinese Holstein cows and associations between SNPs or haplotypes and clinical mastitis infection were analyzed by logistic regression model. Significant associations between rs385343195 (IL-12Râ2 gene) and haplotypes of SNPs rs385343195-rs109216732-rs109571825 (TGC and TAT), and clinical mastitis infection were detected depending on parity, and this provides insight into the complex mechanisms of immunity to infections. Further investigations are required to reveal the mechanisms of IL-12Râ2 and IL-23R in mastitis susceptibility in cattle.

REFERENCES

  1. Alluwaimi AM; Leutenegger C; Farver T; Rossitto P; Smith W; Cullor J. (2003). The cytokine markers in Staphylococcus aureus mastitis of bovine mammary gland. J. Vet. Med. B Infect. Dis. Vet. Public Health. 50:105–111.
  2. Castle SC. (2000). Clinical relevance of age-related immune dysfunction. Clin Infect Dis 31:578–585.
  3. Fulop T; Larbi A; Wikby A; Mocchegiani E; Hirokawa K; Pawelec G. (2005). Dysregulation of T-cell function in the elderly: scientific basis and clinical implications. Drugs Aging, 22:589–603.
  4. Langrish C; McKenzie B; Wilson N; Malefyt R; Kastelein R; Cua D. (2004). IL-12 and IL-23: Master regulators of innate and adaptive immunity. Immunol. Rev. 202:96–105.
  5. Mao YJ, Zhu XR, Xing SY, Zhang MR, Wang XL, Cheng D, Zhang HM, Sven K, Yang ZP, Yang LG. (2015) Polymorphisms in the promoter of interleukin-12 â2 and interleukin-23 receptor genes influence milk production traits in Chinese Holstein cows. Livestock Science. 178:1-8.
  6. Nash DL; Rogers GW; Cooper JB; Hargrove G; Keown JF. (2003). Heritability of intramammary in fections at first parturition relationships with sire transmitting abilities for somatic cell score, udder type traits, productive life, and protein yield. J. Dairy Sci. 86:2684–2695.
  7. Petropoulou C; Chondrogianni N; Simões D; Agiostratidou G; Drosopoulos N; Kotsota V; Gonos ES. (2000). Aging and longevity. A paradigm of complementation between homeostatic mechanisms and genetic control. Ann NY Acad Sci. 908:133–142.
  8. Remmers EF; Cosan F; Kirino Y; Ombrello MJ; Abaci N; Satorius C. (2010). Genome-wide association study identifies variants in the MHC class I, IL10,and IL23R–IL12RB2 regions associated with Behcet’s disease. Nat. Genet. 42:698–702.
  9. Ruegg PL. (2003). Investigation of mastitis problems on farms. Vet Clin North Am Food Anim Pract. 19:47–73
  10. Rupp R; Boichard D. (1999). Genetic parameters for clinical mastitis, somatic cell score, production, udder type and milking easy in first lactation Holsteins. J. Dairy Sci. 82:2198–2204
  11. Shook GE. (2006). Major advances in determining appropriate selection goals. J Dairy Sci. 89:1349–1361.
  12. Skelding A; Schenkel FS; Sharma BS; Verschoor C; Pant S; Biggar G; Boermans H; Karrow N. (2010). Identification of single nucleotide polymorphisms in the bovine interleukin-12 and interleukin-23 receptor genes and their associations with health and production traits in Holstein cows. J. Dairy Sci. 93 :4860–4871.
  13. Sole X; Guino E; Valls J; Iniesta R; Moreno V. (2006). SNPStats: a web tool for the analysis of association studies. Bioinformatics, 22: 1928-1929.
  14. Thompson-Crispi KA; Miglior F; Mallarda BA. (2013). Incidence Rates of Clinical Mastitis among Canadian Holsteins Classified as High, Average, or Low Immune Responders. Clinical and Vaccine Immunology. 20:106–112.
  15. van de Vosse E; Lichtenauer-Kaligis E; Dissel J; Ottenhoff T. (2003). Genetic variations in the interleukin-12/interleukin-23 receptor (â1) chain, and implications for IL-12 and IL-23 receptor structure and function. Immunogenetics. 54:817–829.
  16. Welderufael BG; Lovendahl P; De Koning DJ; Janss LLG; Fikse WF. (2018). Genome-wide association study for susceptibility to and recoverability from mastitis in Danish Holstein cows. Frontiers in Genetics 9: 141.
  17. Wojdak-Maksymiec K; Szyda J; Strabel T. (2013). Parity-dependent association between TNF-á and LTF gene polymorphisms and clinical mastitis in dairy cattle. BMC Veterinary Research. 9:114-121. 
  18. Wolff C; Espetvedt M; Lind AK; Rintakoski S; Egenvall A; Lindberg A; Emanuelson U. (2012). Completeness of the disease recording systems for dairy cows in Denmark, Finland, Norway and Sweden with special reference to clinical mastitis. BMC Veterinary Research, 8:131.
  19. Xavier JM; Shahram F; Davatchi F; Rosa A; Crespo J; Abdollahi BS. (2012). Association study of IL10 and IL23R–IL12RB2 in Iranian patients with Behcet’s disease. Arthritis Rheum. 64:2761–2772. 
  20. Yilmaz V; Yentur S; Saruhan-Direskeneli G. (2005). IL-12 and IL-10 polymorphisms and their effects on cytokine production. Cytokine. 30:188–194. 
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 12 (december 2018) : 1705-1710,   Doi: 10.18805/ijar.B-807

    Parity-dependent association between IL-12Râ2 and IL-23R gene polymorphisms and clinical mastitis in Chinese Holstein cows

    M
    Mengqi Wang
    W
    Wei Ni
    H
    Huimin Zhang
    X
    Xiaolong Wang
    Z
    Zhangping Yang
    E
    Eveline M. Ibeagha-Awemu
    Y
    Yongjiang Mao
    1College of Animal Science and Technology, Yangzhou University, 225 009, Yangzhou, P. R. China.
    Cite article:- Wang Mengqi, Ni Wei, Zhang Huimin, Wang Xiaolong, Yang Zhangping, Ibeagha-Awemu M. Eveline, Mao Yongjiang (2018). Parity-dependent association between IL-12Râ2 and IL-23R gene polymorphisms and clinical mastitis in Chinese Holstein cows. Indian Journal of Animal Research. 52(12): 1705-1710. doi: 10.18805/ijar.B-807.

    ABSTRACT

    Interleukin-12 (IL-12) and interleukin-23 (IL-23) are proinflammatory cytokines produced by macrophages and dendritic cells in response to infection with intracellular pathogens. Given the importance of IL-12 and IL-23 for modulating inflammation and the host immune response, the IL-12 and IL-23 receptor genes may be suitable candidate genes for studying disease resistance in dairy cattle. DNA samples of twenty Chinese Holstein cows were selected randomly for PCR amplification and sequencing, and used for SNP discovery in the bovine IL-12Râ2 and IL-23R promoter regions. One SNP (c.-246G>T, rs385343195) in IL-12Râ2 and 2 SNPs [c.-856A>G (rs109216732) and c.-207T>C (rs109571825)] in IL-23R were identified. Identified SNPs were genotyped in 866 Chinese Holstein cows and associations between SNPs or haplotypes and clinical mastitis infection were analyzed by logistic regression model. Significant associations between rs385343195 (IL-12Râ2 gene) and haplotypes of SNPs rs385343195-rs109216732-rs109571825 (TGC and TAT), and clinical mastitis infection were detected depending on parity, and this provides insight into the complex mechanisms of immunity to infections. Further investigations are required to reveal the mechanisms of IL-12Râ2 and IL-23R in mastitis susceptibility in cattle.

    REFERENCES

    1. Alluwaimi AM; Leutenegger C; Farver T; Rossitto P; Smith W; Cullor J. (2003). The cytokine markers in Staphylococcus aureus mastitis of bovine mammary gland. J. Vet. Med. B Infect. Dis. Vet. Public Health. 50:105–111.
    2. Castle SC. (2000). Clinical relevance of age-related immune dysfunction. Clin Infect Dis 31:578–585.
    3. Fulop T; Larbi A; Wikby A; Mocchegiani E; Hirokawa K; Pawelec G. (2005). Dysregulation of T-cell function in the elderly: scientific basis and clinical implications. Drugs Aging, 22:589–603.
    4. Langrish C; McKenzie B; Wilson N; Malefyt R; Kastelein R; Cua D. (2004). IL-12 and IL-23: Master regulators of innate and adaptive immunity. Immunol. Rev. 202:96–105.
    5. Mao YJ, Zhu XR, Xing SY, Zhang MR, Wang XL, Cheng D, Zhang HM, Sven K, Yang ZP, Yang LG. (2015) Polymorphisms in the promoter of interleukin-12 â2 and interleukin-23 receptor genes influence milk production traits in Chinese Holstein cows. Livestock Science. 178:1-8.
    6. Nash DL; Rogers GW; Cooper JB; Hargrove G; Keown JF. (2003). Heritability of intramammary in fections at first parturition relationships with sire transmitting abilities for somatic cell score, udder type traits, productive life, and protein yield. J. Dairy Sci. 86:2684–2695.
    7. Petropoulou C; Chondrogianni N; Simões D; Agiostratidou G; Drosopoulos N; Kotsota V; Gonos ES. (2000). Aging and longevity. A paradigm of complementation between homeostatic mechanisms and genetic control. Ann NY Acad Sci. 908:133–142.
    8. Remmers EF; Cosan F; Kirino Y; Ombrello MJ; Abaci N; Satorius C. (2010). Genome-wide association study identifies variants in the MHC class I, IL10,and IL23R–IL12RB2 regions associated with Behcet’s disease. Nat. Genet. 42:698–702.
    9. Ruegg PL. (2003). Investigation of mastitis problems on farms. Vet Clin North Am Food Anim Pract. 19:47–73
    10. Rupp R; Boichard D. (1999). Genetic parameters for clinical mastitis, somatic cell score, production, udder type and milking easy in first lactation Holsteins. J. Dairy Sci. 82:2198–2204
    11. Shook GE. (2006). Major advances in determining appropriate selection goals. J Dairy Sci. 89:1349–1361.
    12. Skelding A; Schenkel FS; Sharma BS; Verschoor C; Pant S; Biggar G; Boermans H; Karrow N. (2010). Identification of single nucleotide polymorphisms in the bovine interleukin-12 and interleukin-23 receptor genes and their associations with health and production traits in Holstein cows. J. Dairy Sci. 93 :4860–4871.
    13. Sole X; Guino E; Valls J; Iniesta R; Moreno V. (2006). SNPStats: a web tool for the analysis of association studies. Bioinformatics, 22: 1928-1929.
    14. Thompson-Crispi KA; Miglior F; Mallarda BA. (2013). Incidence Rates of Clinical Mastitis among Canadian Holsteins Classified as High, Average, or Low Immune Responders. Clinical and Vaccine Immunology. 20:106–112.
    15. van de Vosse E; Lichtenauer-Kaligis E; Dissel J; Ottenhoff T. (2003). Genetic variations in the interleukin-12/interleukin-23 receptor (â1) chain, and implications for IL-12 and IL-23 receptor structure and function. Immunogenetics. 54:817–829.
    16. Welderufael BG; Lovendahl P; De Koning DJ; Janss LLG; Fikse WF. (2018). Genome-wide association study for susceptibility to and recoverability from mastitis in Danish Holstein cows. Frontiers in Genetics 9: 141.
    17. Wojdak-Maksymiec K; Szyda J; Strabel T. (2013). Parity-dependent association between TNF-á and LTF gene polymorphisms and clinical mastitis in dairy cattle. BMC Veterinary Research. 9:114-121. 
    18. Wolff C; Espetvedt M; Lind AK; Rintakoski S; Egenvall A; Lindberg A; Emanuelson U. (2012). Completeness of the disease recording systems for dairy cows in Denmark, Finland, Norway and Sweden with special reference to clinical mastitis. BMC Veterinary Research, 8:131.
    19. Xavier JM; Shahram F; Davatchi F; Rosa A; Crespo J; Abdollahi BS. (2012). Association study of IL10 and IL23R–IL12RB2 in Iranian patients with Behcet’s disease. Arthritis Rheum. 64:2761–2772. 
    20. Yilmaz V; Yentur S; Saruhan-Direskeneli G. (2005). IL-12 and IL-10 polymorphisms and their effects on cytokine production. Cytokine. 30:188–194. 
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