Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 54 issue 4 (april 2020) : 478-481

Detection of Brucella antibodies in selected wild animals and avian species in Pakistan

Shahzad Ali, Samra Saleem, Muhammad Imran, Muhammad Rizwan, Khizar Iqbal, Ghulam Qadir, Haroon Ahmad, Sajid Umar, Waseem Ahmad Khan, Iahtasham Khan, Heinrich Neubauer
1Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
Cite article:- Ali Shahzad, Saleem Samra, Imran Muhammad, Rizwan Muhammad, Iqbal Khizar, Qadir Ghulam, Ahmad Haroon, Umar Sajid, Khan Ahmad Waseem, Khan Iahtasham, Neubauer Heinrich (2018). Detection of Brucella antibodies in selected wild animals and avian species in Pakistan. Indian Journal of Animal Research. 54(4): 478-481. doi: 10.18805/ijar.B-799.
Brucellosis is a zoonotic disease which affects humans, farm animals and wildlife as well. In Pakistan, the status of human and farm animal brucellosis is documented but the information about brucellosis in wildlife is lacking. To access the seroprevalence of Brucella antibodies in avian (turkeys, peafowl, guineafowl, mallard ducks and Indian blue rock pigeon) and selected wild species (reptiles and amphibians), a total of 117 serum samples (reptile = 34, amphibian = 04, avian n = 79) were collected from Karachi and Pattoki regions of Pakistan. Serum samples were screened for Brucella antibodies using Rose Bengal plate test (RBPT) and11.1% serum samples were found positive. Overall, 2.5% birds, 29.4% reptiles and 25% amphibians were seropositive for Brucella antibodies. This is first report of detection of Brucella antibodies in reptiles and amphibians in Pakistan. 
  1. Adamu, N.B., Adamu. S.G., Jajere, M.S., Atsanda, N.N., Mustapha, F.B., Maina, M. (2014). Serological survey of brucellosis in slaughtered local chicken, guinea fowl, ducks and turkey in North-Eastern Nigeria. International Journal of Poultry Science, 13: 340-342.
  2. Ahmad, H. and Munir, M.A. (1995). Epidemiological investigation of brucellosis in Pakistan. Pakistan Veterinary Journal, 15: 169-172.
  3. Alaga, A.A., Ogah, D.M., Attah, J. (2012). Seroprevalence of brucellosis in some poultry species in Nasarawa state, Nigeria. Egyptian Poultry Science, 32: 705-709.
  4. Al Dahouk, S., K. N €ockler, H. Tomaso, W. D. Splettstoesser, G.Jungersen, U. Riber, T. Petry, D. Hoffmann, H. C. Scholz, A. Hensel, and H. Neubauer, (2005): Seroprevalence of brucellosis, tularemia, and yersiniosis in wild boars (Sus scrofa ) from north-    eastern Germany. J. Vet. Med. B Infect. Dis. Vet. Public Health 52, 444–455.
  5. Ali, S., Ali, Q., Neubauer, H., Melzer, F., Elschner, M., Khan, I., Abatih, E., Ullah, N., Irfan, M., Akhter, S. (2013). Seroprevalence and risk factors associated with Brucellosis as a professional hazard in Pakistan. Foodborne Pathogens and Disease, 10: 500-505.
  6. Ali, S., Akhter, S., Neubauer, H., Melzer, F., Khan, I., Ali, Q., Irfan, M. (2015). Serological, cultural, and molecular evidence of Brucella infection in small ruminants, Pakistan. Journal of Infection in Developing Countries, 9: 470-475.
  7. Ali, S., Akhter, S., Neubauer, H., Scherag, A., Kesselmeier, M., Melzer, F., Khan, I., El-Adawy, H., Azam, A., Qadeer, S., Ali, Q. (2016). Brucellosis in pregnant women from Pakistan: an observational study. BMC Infectious Diseases, 16: 468.
  8. Ali, S., Akhter, S., Neubauer, H., Melzer, F., Khan, I., Abatih, E.N., El Adawy, H., Irfan, M., Muhammad, A., Akbar, M.W., Umar, S., Ali, Q., Iqbal, M.N., Mahmood, A., Ahmed, H. (2017). Seroprevalence and risk factors associated with bovine brucellosis in the Potohar Plateau, Pakistan. BMC Research Notes, 10: 73.
  9. Alton, G.G., Jones, L.M., Angus, R.D., Verger, J.M. (1988). Bacteriological methods. In: Alton GG, Jones LM, Angus RD, Verger JM, editors. Techniques for the Brucellosis Laboratory. Paris: Institut National De La Recherche Agronomique. pp. 13-61.
  10. Aune, K., Rhyan, J.C., Russell, R., Roffe, T.J., Corso, B. (2011). Environmental Persistence of Brucella abortus in the Greater Yellowstone Area. Journal of Wildlife Management, 9999:1-9.
  11. Bale, J.O. and Nuru, S. (1982). Serological survey of brucellosis in Northern Nigeria. Journal of Animal Production Research, 1: 56-62.
  12. Dominik, F., Lorenz, N., Heuser, W., Kampfer, P., Scholz, H.C., Lierz, M. (2012). Abscesses associated with a Brucella inopinata-like bacterium in a big-eyed tree frog (Leptopelis vermiculatus). Journal of Zoo and Wildlife Medicine, 43: 625-628.
  13. Eisenberg, T., Hamann, H.P., Kaim, U., Schlez, K., Seeger, H., Schauerte, N., Melzer, F., Tomaso, H., Scholz, H.C., Koylass, M.S., Whatmore, A.M., Zschock, M. (2012). Isolation of Potentially Novel Brucella spp. from Frogs. Applied Environmental Microbiology, 78: 3753-3755. 
  14. El-Tras, W.F., Tayel, A.A., Eltholth, M.M., Guitian, J. (2010). Brucella infection in fresh water fish: Evidence for natural infection of Nile catfish, Clarias gariepinus, with Brucella melitensis. Veterinary Microbiology, 141: 321-325.
  15. Gugong, V.T., Maurice, N.A., Ngbede, E.O., Hambolu, S.M., Ajogi, I. (2012). Serological evidence of brucellosis in local chickens in Kaduna State, Nigeria. Journal of Animal and Veterinary Advances, 11: 418-419.
  16. Hammer, J.A., Ulrich, R.G., Imholt, C., Scholz, H.C., Jacob, J., Kratzmann, N., Nockler, K., Al-Dahouk, S. (2017). Molecular Survey on Brucellosis in Rodents and Shrews - Natural Reservoirs of Novel Brucella Species in Germany. Transboundary and Emerging Diseases, 64: 663-671.
  17. Kaur, P., Sharma, N.S., Arora, A.K., Deepti. (2007). Investigation of brucellosis in cattle and buffaloes by conventional and molecular assays. Indian Journal of Animal Research, In Press.
  18. Kumar, V., Maan, S., Kumar, A., Batra, K., Chaudhary, D., Dalal, A., Gupta, A.K., Bansal, N., Sheoran, N., Maan, N.S. (2017). Real time PCR assay for differentiation of Brucella abortus and Brucella melitensis. Indian Journal of Animal Research, DOI: 10.18805/ijar.v0iOF.8464 
  19. Mantur, B.G., Biradar, M.S., Bidri, R.C., Mulimani, M.S., Veerappa., Kariholu, P., Patil, S.B., Mangalgi, S.S. (2006). Protean clinical manifestations and diagnostic challenges of human brucellosis in adults, 16 years’ experience in endemic area. Journal of Medical Microbiology, 55: 897-903.
  20. Natacha, W., Carlos, A., Andreas, T., Eleonore, G., Marcus, G.D., Patrick, B., Marie, P., Degiorgis, R. 2012. Risk factors for contacts between wild boar and outdoor pigs in Switzerland and investigations on potential Brucella suis spill-over. BMC Veterinary Research, 8: 116. 
  21. Pavlin, B.I., Lisa, M., Schloegel., Peter, D. (2009). Risk of Importing Zoonotic Diseases through Wildlife Trade, United States. Emerging Infectious Diseases, 15: 1721-1726. 
  22. Radostits, O.M., Gay, C.C., Hinchcliff, K.W., Constable, P.D. (2007). Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats. Saunders Elsevier, Edinburgh. pp. 963-984.
  23. Scholz, H. C., Hubalek, Z., Nesvadbova, J., Tomaso, H., Vergnaud, G., Le-Fleche, P., Whatmore, A.M., Al-Dahouk, S., Kruger, M., Lodri, C., Pfeffer, M. (2008). Isolation of Brucella microti from soil. Emerging Infectious Diseases, 14: 1316-1317.
  24. Scholz, H. C., E. Hofer, G. Vergnaud, P. Le Fleche, A. M. What-more, S. Al Dahouk, M. Pfeffer, M. Kr€uger, A. Cloeckaert, and H. Tomaso, 2009b: Isolation of Brucella microti from mandibular lymph nodes of red foxes, Vulpes vulpes, in lower Austria. Vector Borne Zoonotic Dis. 9, 153–156.
  25. Shehu, L.M. and Ehitade, E.C. (1999). Serological survey of brucellosis in local chickens, guinea fowl, ducks and turkey in Bauchi and Environs. Nigerian Veterinary Journal, 20: 4747-4750.
  26. Song, X., Li, K., Zhang, H., Lan, Y., Luo, H. (2016). Brucella infection in goats in Hubei province, China. Indian Journal of Animal Research, DOI: 10.18805/ijar.v0iOF.4559.
  27. Whatmore, A.M., Emma-Jane, D., Emma, S., Jakub, M., Mark, K., Claire, D., Krishna, K.G., Lorraine, L.P., Matthew, J., Alistair, L. (    2015). Isolation of Brucella from a Whites tree frog (Litoria caerulea). JMM Case Reports, DOI 10.1099/jmmcr.0.000017. 

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