Effect of Microencapsulated Butyric Acid supplementation on growth performance, ileal digestibility of protein, gut health and immunity in Broilers

DOI: 10.18805/ijar.B-788    | Article Id: B-788 | Page : 1618-1622
Citation :- Effect of Microencapsulated Butyric Acid supplementation on growth performance, ileal digestibility of protein, gut health and immunity in Broilers.Indian Journal Of Animal Research.2018.(52):1618-1622
M. Imran, S. Ahmed, Y.A. Ditta, S. Mehmood, Z. Rasool and M.W. Zia doctorwsi639@gmail.com
Address : Department of Animal Nutrition, University of Veterinary and Animal Sciences, Lahore-54000, Pakistan
Submitted Date : 12-06-2017
Accepted Date : 23-10-2017


The effect of microencapsulated butyric acid (MEBA) on growth performance, ileal digestibility (ID) of protein, gut health and immunity was assessed in broilers. A total of 336 d-old Hubbard Classic broilers chicks were randomly assigned to four dietary treatments (Control, 0.25, 0.35 and 0.45 g/kg of MEBA). Each treatment was replicated three times with 28 birds/replicate. Feed intake, body weight gain, FCR, intestinal morphology, ID of protein and immunity was evaluated. At 35-d of age, three birds/replicate were randomly selected and slaughtered to collect blood, duodenal samples, and ileal digesta. The Improved body weight gain (P = 0.05), FCR (P<0.01), duodenal villous height (P<0.05) and ID (P<0.1) were observed; whereas, unresponsive treatment effect with respect to feed intake (P>0.5) and antibody titer against Newcastle disease (P>0.05) were noted. The results indicated that MEBA improves digestion and absorptive processes which consequently improved the broilers performance.


Broilers performance Gut health Immunity Ileal digestibility Microencapsulated butyric acid


  1. AOAC. (2000). Official Methods of Analysis. 14th Edn. Association of Official Analytical Chemists, Washington, DC.
  2. Abdel-Fattah, S.A., El-Sanhoury, M.H., El-Mednay, N.M., Abdel-Azeem, F. (2008). Thyroid activity, some blood constituents, organs morphology and performance of broiler chicks fed supplemental organic acids. International Journal of Poultry Sciences, 7: 215-222.
  3. Adil, S., Banday, T., Ahmad Bhat, G., Salahuddin, M., Raquib, M., Shanaz, S. (2011). Response of broiler chicken to dietary supplementation of organic acids. Journal of Central European Agriculture, 12: 498-508.DOI: 10.5513/JCEA01/12.3.947.
  4. Aghazadeh, A.M. and Taha Yazdi, M. (2012). Effect of butyric acid supplementation and whole wheat inclusion on the performance and carcass traits of broilers. South African Journal of Animal Sciences, 42: 241-248.
  5. Bartholome, B., Spies, C.M., Gaber, T., Schuchmann, S., Berki, T., Kunkel, D., Bienert, M., Radbruch, A., Burmester, G.R., Lauster, R. (2004). Membrane glucocorticoid receptors (mGCR) are expressed in normal human peripheral blood mononuclear cells and up-regulated after in vitro stimulation and in patients with rheumatoid arthritis. FASEB Journal, 18: 70-80.DOI: 10.1096/    fj.03-0328com.
  6. Chamba, F., Puyalto, M., Ortiz, A., Torrealba, H., Mallo, J., Riboty, R. (2014). Effect of partially protected sodium butyrate on performance, digestive organs, intestinal villi and E. coli development in broilers chickens. International Journal of Poultry Sciences, 13: 390-396.
  7. Chen, X., Naehrer, K. and Applegate, T. (2016). Interactive effects of dietary protein concentration andaflatoxin B1 on performance, nutrient digestibility, and gut health in broiler chicks. Poultry Science, 95: 1312-1325.
  8. Choct, M. (2009). Managing gut health through nutrition. British Poultry Science, 50: 9-15.
  9. Dalmasso, G., Nguyen, H.T., Yan, Y., Charrier-Hisamuddin, L., Sitaraman S.V. and Merlin, D. (2008). Butyrate transcriptionally enhances peptide transporter PepT1 expression and activity. PLoS One, 3: 2476.DOI: 10.1371/journal.pone.0002476.
  10. Dehghani-Tafti, N. Jahanian, R. (2016). Effect of supplemental organic acids on performance, carcass characteristics, and serum biochemical metabolites in broilers fed diets containing different crude protein levels. Anim. Feed Science and Technology, 211: 109-116.
  11. Duncan, D.B. (1955). Multiple range and multiple F tests. Biometrics. 11: 1-42.
  12. Eshak, M., Elmenawey, M., Atta, A., Gharib, H., Shalaby, B. and Awaad, M. (2016). The efficacy of Na-butyrate encapsulated in palm fat on performance of broilers infected with necrotic enteritis with gene expression analysis. Veterinary World, 9: 450-457.
  13. Guilloteau, P., Zabielski, R., David, J., Blum, J., Morisset, J., Biernat, M., Woliñski, J., Laubitz D. and Hamon, Y. (2009). Sodium-    butyrate as a growth promoter in milk replacer formula for young calves. Journal of Dairy Sciences, 92: 1038-1049.
  14. Hussain, J., Rabbani, I., Aslam, S. and Ahmad, H. (2015). An overview of poultry industry in Pakistan. World Poultry Science Journal, 71: 689-700.
  15. Jahanian, R. (2011). Effect of varying levels of butyric acid glycerides on performance, immune responses and jejuna epithelium morphology of broiler chicks. In 18th European Symposium on Poultry Nutrition, Izmir, Turkey. pp. 213-215.
  16. Jahanian, R. and Golshadi, M. (2015). Effect of dietary supplementation of butyric acid glycerides on performance, immunological responses, ilealmicroflora, and nutrient digestibility in laying hens fed different basal diets. Livestock Science, 178: 228-236.
  17. Kaczmarek, S., Barri, A., Hejdysz, M. and Rutkowski, A. (2016). Effect of different doses of coated butyric acid on growth performance and energy utilization in broilers. Poultry Science, 95: 851-859.
  18. Leeson, S., Namkung, H., Antongiovanni, M. and Lee, E. (2005). Effect of butyric acid on the performance and carcass yield of broiler chickens. Poultry Science, 84: 1418-1422.
  19. Lemme, A., Ravindran, V. and Bryden, W.L. (2004). Ileal digestibility of amino acids in feed ingredients for broilers. World Poultry Science Journal, 60: 423-438.
  20. Levy, A.W., Kessler, J.W., Fuller, L., Williams, S., Mathis, G.F., Lumpkins, B., Valdez, F. (2015). Effect of feeding an encapsulated source of butyric acid (ButiPEARL) on the performance of male Cobb broilers reared to 42 d of age. Poultry Science, 94: 1864-1870.
  21. Mahdavi, R. and Torki, M. (2009). Study on usage period of dietary protected butyric acid on performance, carcass characteristics, serum metabolite levels and humoral immune response of broiler chickens. Journal of Animal and Veterinary Advances, 8: 1702-1709.
  22. Niewold, T.A. (2014). Why anti-inflammatory compounds are the solution for the problem with in feed antibiotics. Quality Assurance and Safety of Crops & Foods, 6: 119-122.
  23. NRC, (1994). Nutrient Requirements of Poultry. 9th rev. Edn. National Academy Press, Washington, 
  24. Panda, A.K., Rao, S.V.R., Raju, M.V.L.N. and Sunder, G.S. (2009). Effect of butyric acid on performance, gastrointestinal tract health and carcass characteristics in broiler chickens. Asian-Australian Journal of Animal Science, 22: 1026-1031.
  25. Qaisrani, S.N., van, Krimpen, M.M., Kwakkel, R.P., Verstegen, M.W.A. and Hendriks, W.H. (2015). Diet structure, butyric acid, and fermentable carbohydrates influence growth performance, gut morphology, and cecal fermentation characteristics in broilers. Poultry Science, 94: 2152-2164.
  26. Ravindran, V., Morel, P., Partridge, G., Hruby, M. and Sands, J. (2006). Influence of an Escherichia coli-derived phytase on nutrient utilization in broiler starters fed diets containing varying concentrations of phytic acid. Poultry Science, 85: 82-89.
  27. Roberts, T., Wilson, J., Guthrie, A., Cookson, K., Vancraeynest, D., Schaeffer, J., Moody, R. and Clark, S. (2015). New issues and science in broiler chicken intestinal health: Emerging technology and alternative interventions. Journal of Applied Poultry Research, 24: 257-266.
  28. Sileikiene, V., Mosenthin, R., Tafaj, M., Kruszewska, D., Weström, B., Mattsson, I. and Pierzynowski, S. (2005). Effect of short chain fatty acids infused intraileally on interdigestive exocrine pancreatic secretions in growing pigs. Journal Animal Physiology and Animal Nutrition, 89: 253-259.
  29. Smith, D.J., Barri, A., Herges, G., Hahn, J., Yersin, A.G. and Jourdan, A. (2012). In vitro dissolution and in vivo absorption of calcium [1-14C] butyrate in free or protected forms. Journal of Agriculture and Food Chemistry, 60: 3151-3157.
  30. Smulikowska, S., Czerwiñski, J., Mieczkowska, A. and Jankowiak, J. (2009). The effect of fat-coated organic acid salts and a feed enzyme on growth performance, nutrient utilization, microflora activity, and morphology of the small intestine in broiler chickens. Journal of Animal and Feed Sciences, 18: 478-489.
  31. Steel, R.G.D., Torrie, J.H. and Dickey, D.A. (1997). Principles and procedures of statistics a biometrical approach, McGraw Hill Book Co. Inc. New York, USA.
  32. Taherpour, K. and Ghasemi, H.A. (2014). Single and Combinative Effects of Fructo-oligosaccharide and Organic Acid on Growth Performance and Immune Function of Broiler Chickens. Agriculture Communication, 2: 67-72. 

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