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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 7 (july 2019) : 880-885,   Doi: 10.18805/ijar.B-774

New polymorphisms of PAPPA and PAPPA2 genes and their associations with egg production traits in Chinese Dagu chickens

D
D. Liu
X
X. Niu
T
T.L. Tyasi
N
N. Qin
H
H. Zhu
X
X. Chen
R
R. Xu
1Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun-130118, China.
Cite article:- Liu D., Niu X., Tyasi T.L., Qin N., Zhu H., Chen X., Xu R. (2017). New polymorphisms of PAPPA and PAPPA2 genes and their associations with egg production traits in Chinese Dagu chickens. Indian Journal of Animal Research. 53(7): 880-885. doi: 10.18805/ijar.B-774.

ABSTRACT

Pregnancy-associated plasma protein A, pappalysin1 (PAPPA) and pappalysin2 (PAPPA2) genes were implicated in regulation of hen ovarian follicular development and growth. Four novel single nucleotide polymorphisms (SNPs) were identified using PCR-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing. Among them, A/G transition at position 172864 and T/C mutation at position 172952 in 3‘-untranslated region (UTR) of PAPPA named SNP A172864G and T172952C, respectively. A/G transition at position 77421 and T/C at position 77455 in 3‘-UTR of PAPPA2 gene named as SNP A77421G and T77455C, respectively. For SNP A172864G and T172952C (PAPPA), 360 Dagu hens were classified as AA, AB and BB genotypes based on PCR-SSCP patterns, and BB genotype correlated significantly (P<0.05) with higher hen-housed egg production (HHEP) at 30 and 57 weeks (wks) of age and higher egg weight (EW) at 43 wks of age. Consequently, these SNPs identified will be potential genetic markers to improve egg productivity in chicken breeding.

REFERENCES

  1. Almasy, L. and Blangero, J. (2010). Variance component methods for analysis of complex phenotypes. Cold Spring Harbor Protocols. 5: pdb.top77. doi: 10.1101/pdb.top77.
  2. Conover, C.A. (2012). Key questions and answers about pregnancy-associated plasma protein-A. Trends in Endocrinology and Metabolism, 23 (5): 242-249. 
  3. Conover, C.A., Faessen, G.F., Ilg, K.E., Chandrasekher, Y.A., Christiansen, M., Overgaard, M.T., Oxvig, C., Giudice, L.C. (2001). Pregnancy-associated plasma protein-a is the insulin-like growth factor binding protein-4 protease secreted by human ovarian granulosa cells and is a marker of dominant follicle selection and the corpus luteum. Endocrinology, 142 (5): 21-55.
  4. Falconer, D.S., Trudy, F.C., Mackay, T.F.C. (1996). Introduction to Quantitative Genetics. 4th edition. Longmans Green: Harlow, Essex, UK.
  5. Hourvitz, A., Kuwahara, A., Hennebold, J.D., Tavares, A.B., Negishi, H., Lee, T.H., Erickson, G.F., Adashi, E.Y. (2002). The regulated expression of the pregnancy-associated plasma protein-A in the rodent ovary: a proposed role in the development of dominant follicles and of corpora lutea. Endocrinology, 143 (5): 1833-1844.
  6. Luna-Nevarez, P., Rincon, G., Medrano, J.F., Riley, D.G., Chase, C.C., Coleman, S.W., Vanleeuwen, D. M., DeAtley, K.L., Islas-Trejo, A., Silver, G.A., Thomas, M.G. (2011). Single nucleotide polymorphisms in the growth hormone-insulin-like growth factor axis in straight bred and crossbred Angus, Brahman, and Romosinuano heifers: population genetic analyses and association of genotypes with reproductive phenotypes. Journal of Animal Science, 89 (4): 926-934.
  7. Mazerbourg, S., Overgaard, M.T., Oxvig, C., Christiansen, M., Conover, C.A., Laurendeau, I., Vidaud, M., et al. (2001). Pregnancy-    associated plasma protein-A (PAPP-A) in ovine, bovine, porcine, and equine ovarian follicles: involvement in IGF binding protein-4 proteolytic degradation and mRNA expression during follicular development. Endocrinology. 142 (12): 5243-5253.
  8. Overgaard, M.T., Boldt, H.B., Laursen, L.S., Sottrup-Jensen, L., Conover, C.A., Oxvig, C. (2001). Pregnancy-associated plasma protein-A2 (PAPP-A2), a novel insulin-like growth factor-binding protein-5 proteinase. Journal of Biological Chemistry, 276 (24): 21849-21853.
  9. Qin, N., Liu, Q., Zhang, Y.Y., Fan, X.C., Xu, X.X., Lv, Z.C., Wei, M.L., Jing, Y., Mu, F., Xu, R.F. (2015). Association of novel polymorphisms of forkhead box L2 and growth differentiation factor-9 genes with egg production traits in local Chinese Dagu hens. Poultry Science, 94 (1): 88-95. 
  10. Sambrook, J. and Russell, R.W. (2001). Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  11. Stephens, M., Smith, N.J., Donnelly, P. (2001). A new statisticalmethod for haplotype reconstruction from population data. American Journal of Human Genetics, 68: 978-989.
  12. Tyasi, T.L., Qin, N., Jing, Y., Mu, F., Zhu, H., Liu, D., Yuan, S. and Xu, R. (2017). Assessment of relationship between body weight and body measurement traits of indigenous Chinese Dagu chickens using path analysis. Indian Journal of Animal Research, 51(3): 588-593. 
  13. Wickramasinghe, S., Rincon, G., Medrano, J.F. (2011). Variants in the pregnancy-associated plasma protein-A2 gene on Bos taurus autosome 16 are associated with daughter calving ease and productive life in Holstein cattle. Journal of Dairy Science, 94 (3): 1552-1558.
  14. Yeh, F.C., Yang, R.C., Boyle, T.B.J., Ye, Z.H., Mao, J.X. (1997). POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Alberta, Canada.
  15. Zhang, L., Li, D.Y., Liu, Y.P., Wang, Y., Zhao, X.L., Zhu, Q. (2012). Genetic effect of the prolactin receptor gene on egg production traits in chickens. Genetics and Molecular Research, 11: 4307-4315.
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 7 (july 2019) : 880-885,   Doi: 10.18805/ijar.B-774

    New polymorphisms of PAPPA and PAPPA2 genes and their associations with egg production traits in Chinese Dagu chickens

    D
    D. Liu
    X
    X. Niu
    T
    T.L. Tyasi
    N
    N. Qin
    H
    H. Zhu
    X
    X. Chen
    R
    R. Xu
    1Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Jilin Agricultural University, Changchun-130118, China.
    Cite article:- Liu D., Niu X., Tyasi T.L., Qin N., Zhu H., Chen X., Xu R. (2017). New polymorphisms of PAPPA and PAPPA2 genes and their associations with egg production traits in Chinese Dagu chickens. Indian Journal of Animal Research. 53(7): 880-885. doi: 10.18805/ijar.B-774.

    ABSTRACT

    Pregnancy-associated plasma protein A, pappalysin1 (PAPPA) and pappalysin2 (PAPPA2) genes were implicated in regulation of hen ovarian follicular development and growth. Four novel single nucleotide polymorphisms (SNPs) were identified using PCR-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing. Among them, A/G transition at position 172864 and T/C mutation at position 172952 in 3‘-untranslated region (UTR) of PAPPA named SNP A172864G and T172952C, respectively. A/G transition at position 77421 and T/C at position 77455 in 3‘-UTR of PAPPA2 gene named as SNP A77421G and T77455C, respectively. For SNP A172864G and T172952C (PAPPA), 360 Dagu hens were classified as AA, AB and BB genotypes based on PCR-SSCP patterns, and BB genotype correlated significantly (P<0.05) with higher hen-housed egg production (HHEP) at 30 and 57 weeks (wks) of age and higher egg weight (EW) at 43 wks of age. Consequently, these SNPs identified will be potential genetic markers to improve egg productivity in chicken breeding.

    REFERENCES

    1. Almasy, L. and Blangero, J. (2010). Variance component methods for analysis of complex phenotypes. Cold Spring Harbor Protocols. 5: pdb.top77. doi: 10.1101/pdb.top77.
    2. Conover, C.A. (2012). Key questions and answers about pregnancy-associated plasma protein-A. Trends in Endocrinology and Metabolism, 23 (5): 242-249. 
    3. Conover, C.A., Faessen, G.F., Ilg, K.E., Chandrasekher, Y.A., Christiansen, M., Overgaard, M.T., Oxvig, C., Giudice, L.C. (2001). Pregnancy-associated plasma protein-a is the insulin-like growth factor binding protein-4 protease secreted by human ovarian granulosa cells and is a marker of dominant follicle selection and the corpus luteum. Endocrinology, 142 (5): 21-55.
    4. Falconer, D.S., Trudy, F.C., Mackay, T.F.C. (1996). Introduction to Quantitative Genetics. 4th edition. Longmans Green: Harlow, Essex, UK.
    5. Hourvitz, A., Kuwahara, A., Hennebold, J.D., Tavares, A.B., Negishi, H., Lee, T.H., Erickson, G.F., Adashi, E.Y. (2002). The regulated expression of the pregnancy-associated plasma protein-A in the rodent ovary: a proposed role in the development of dominant follicles and of corpora lutea. Endocrinology, 143 (5): 1833-1844.
    6. Luna-Nevarez, P., Rincon, G., Medrano, J.F., Riley, D.G., Chase, C.C., Coleman, S.W., Vanleeuwen, D. M., DeAtley, K.L., Islas-Trejo, A., Silver, G.A., Thomas, M.G. (2011). Single nucleotide polymorphisms in the growth hormone-insulin-like growth factor axis in straight bred and crossbred Angus, Brahman, and Romosinuano heifers: population genetic analyses and association of genotypes with reproductive phenotypes. Journal of Animal Science, 89 (4): 926-934.
    7. Mazerbourg, S., Overgaard, M.T., Oxvig, C., Christiansen, M., Conover, C.A., Laurendeau, I., Vidaud, M., et al. (2001). Pregnancy-    associated plasma protein-A (PAPP-A) in ovine, bovine, porcine, and equine ovarian follicles: involvement in IGF binding protein-4 proteolytic degradation and mRNA expression during follicular development. Endocrinology. 142 (12): 5243-5253.
    8. Overgaard, M.T., Boldt, H.B., Laursen, L.S., Sottrup-Jensen, L., Conover, C.A., Oxvig, C. (2001). Pregnancy-associated plasma protein-A2 (PAPP-A2), a novel insulin-like growth factor-binding protein-5 proteinase. Journal of Biological Chemistry, 276 (24): 21849-21853.
    9. Qin, N., Liu, Q., Zhang, Y.Y., Fan, X.C., Xu, X.X., Lv, Z.C., Wei, M.L., Jing, Y., Mu, F., Xu, R.F. (2015). Association of novel polymorphisms of forkhead box L2 and growth differentiation factor-9 genes with egg production traits in local Chinese Dagu hens. Poultry Science, 94 (1): 88-95. 
    10. Sambrook, J. and Russell, R.W. (2001). Molecular Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
    11. Stephens, M., Smith, N.J., Donnelly, P. (2001). A new statisticalmethod for haplotype reconstruction from population data. American Journal of Human Genetics, 68: 978-989.
    12. Tyasi, T.L., Qin, N., Jing, Y., Mu, F., Zhu, H., Liu, D., Yuan, S. and Xu, R. (2017). Assessment of relationship between body weight and body measurement traits of indigenous Chinese Dagu chickens using path analysis. Indian Journal of Animal Research, 51(3): 588-593. 
    13. Wickramasinghe, S., Rincon, G., Medrano, J.F. (2011). Variants in the pregnancy-associated plasma protein-A2 gene on Bos taurus autosome 16 are associated with daughter calving ease and productive life in Holstein cattle. Journal of Dairy Science, 94 (3): 1552-1558.
    14. Yeh, F.C., Yang, R.C., Boyle, T.B.J., Ye, Z.H., Mao, J.X. (1997). POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton, Alberta, Canada.
    15. Zhang, L., Li, D.Y., Liu, Y.P., Wang, Y., Zhao, X.L., Zhu, Q. (2012). Genetic effect of the prolactin receptor gene on egg production traits in chickens. Genetics and Molecular Research, 11: 4307-4315.
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