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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 5 (may 2019) : 609-615,   Doi: 10.18805/ijar.B-759

The architecture of the lymph nodes in the abdominal and thoracic cavities of wild boar

Y
Yeþim Akaydin Bozkurt
S
Sevinç Ateþ
T
Tolunay Kozlu
F
Feyza Baþak
1Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Histology and Embryology, Hatay, TURKEY.
Cite article:- Bozkurt Akaydin Yeþim, Ateþ Sevinç, Kozlu Tolunay, Baþak Feyza (2017). The architecture of the lymph nodes in the abdominal and thoracic cavities of wild boar. Indian Journal of Animal Research. 53(5): 609-615. doi: 10.18805/ijar.B-759.

ABSTRACT

The distrubition of lymph nodes located in the abdominal and thoracic cavities of ten wild boars, and their structure were determined anatomically, histologically and immunohistochemically, to be the first detailed investigation on the wild boar. Though general localization and distribution were similar, the number of lymph nodes showed small differences from those of domestic pig. Histological investigations did not reveal a significant hilus. Besides, T lymphocytes with anti-CD3, CD4, CD8, B lymphocytes with anti-CD79a, macrophages with anti-macrophage monoclonal antibodies, and follicular dendritic cells using anti-S100 polyclonal antibody and their distrubution in the lymph nodes were detected. Many CD3 positive T lymphocytes were observed in the germinal center of the lymph follicles, in the cortical area and in the medulla. CD8 positive T lymphocytes were few, and CD4 positive T lymphocytes were not seen. CD79 positive cells were scanty.

REFERENCES

  1. Bacha WJ and Bacha LM (2000). Color Atlas of Veterinary Histology. 2nd ed. Lippincott Williams & Wilkins. Philadelphia, 69-71. 
  2. Bancroft JD and Cook HC (1984). Manual of Histological Techniques. Churchill Livingstone. Edinburgh, London, Melbourne, and New York, 49-50, 83.
  3. Chianini F, Majo N, Segales J, Dominguez J and Domingo M (2001). Immunohistological study of the immune system cells in paraffin-embedded tissues of conventional pigs. Vet Immunol Immunopathol 82: 245-255. 
  4. Denk H, Künzele H, Plenk H, Rüschoff J and Sellner W (1989). Romeis Mikroskopische Technik. 17. Neubearbeitete Auflage. Urban und Schwarzenberg. München, 439-450.
  5. Dursun N (1999). Veteriner Anatomi II. Medisan Yayinevi. Ankara, 293-301.
  6. Dyce KM, Sack WO and Wensing CJG (2002). Textbook of Veterinary Anatomy. Saunders. Philedelphia. 
  7. Eurell JA and Frappier BL (2006). Dellmann’s Textbook of Veterinary Histology. 6th ed. Blackwell Publishing. Ames, Iowa, USA, 143-147. 
  8. Gutierrez M, Forster FI, McConnell SA, Cassidy JP, Pollock JM and Bryson DG (1999). The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet Immunol Immunopathol 71: 321–334.
  9. Heizmann CW, Fritz G and Schafer BW (2002). S100 proteins: structure, functions and pathology. Front Biosci 1(7): 1356-1368.
  10. Hoshi N, Hashimoto Y, Kitagawa H, Kon Y and Kudo N (1986). Histological and immunohistochemical studies on the architecture of lymph nodes in pig. Jpn J Vet Sci 48(6): 1097-1107.
  11. Jonjic N, Jonjic S, Saalmüller A, Rukavina D and Koszinowski UH (1987). Distribution of T-lymphocyte subsets in porcine lymphoid tissues. Immunology 60: 395-401.
  12. Junqueira LC and Carneiro J (2006). Basic Histology. 10th ed. Translation: Y Aytekin and S Solakoðlu. Nobel Týp Kitabevleri. Ankara, 278-284.
  13. Kalita A, Kalita PC and Doley PJ (2014). Light microscopic study on the peripheral lymphnodes of mizo local pig (zo vawk). AJBPS 4(28): 4-8.
  14. Nomina Anatomica Veterinaria (NAV) (2012). International Committee on Veterinary Gross Anatomical Nomenclature and General Assembly of the World Association of Veterinary Anatomists. 5th ed. Published by the Editorial Committee Hannover (Germany), Columbia, MO (U.S.A.), Ghent (Belgium), Sapporo (Japan). 
  15. Ramos JA, Ramis AJ, Rabanal RM, Marco A, Domingo M and Ferrer LM (1990). Scaning electron microscopy of swine lymphoid organs. Histol Histopathol 5: 397-406.
  16. Saar LI and Getty R (1963). The lymph nodes and the lymph vessels of the abdominal wall, pelvic wall and the pelvic limb of swine. Iowa State University Veterinarian 26(2): 97-113.
  17. Sarsilmaz M, Gümüþalan Y, Çelik HH, Gezici M and Akþit MD (1994). Classification of the thoracic and abdominal lymph nodes of the rat. Turk J Med Res 12(b): 185-191.
  18. Schummer A, Wilkens H, Vollmerhaus B and Habermehl KH (1981). The Circulatory System, The Skin, and The Cutaneous Organs of The Domestic Mammals. Verlag Paul Parey. Hamburg.
  19. Spalding HJ and Heath TJ (1989). Fine structure of lymph pathways in nodes from the superficial inguinal lymph centre in the pig. J Anat 166: 43-54.
  20. Sugimura M, Shirogane D, Atoji Y, Suzuki Y, Ohshima Y, Kon Y and Hashimoto Y (1990). A comparative study on S-100 protein-    immunoreactive cells in lymph nodes. Jpn J Vet Sci 52(5): 1015-1021. 
  21. Tipirdamaz S (2000). Lenfatik Sistem. Sel-ün Vakfi Yayinlari. Konya, 57-82.
  22. Tingstedt JE, Tornehave D, Lind P and Nielsen J (2003). Immunohistochemical detection of SWC3, CD2, CD3, CD4 and CD8 antigens in paraformaldehyde fixed and paraffin embedded porcine lymphoid tissue. Vet Immunol Immunopathol 94: 123-132. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 5 (may 2019) : 609-615,   Doi: 10.18805/ijar.B-759

    The architecture of the lymph nodes in the abdominal and thoracic cavities of wild boar

    Y
    Yeþim Akaydin Bozkurt
    S
    Sevinç Ateþ
    T
    Tolunay Kozlu
    F
    Feyza Baþak
    1Mustafa Kemal University, Faculty of Veterinary Medicine, Department of Histology and Embryology, Hatay, TURKEY.
    Cite article:- Bozkurt Akaydin Yeþim, Ateþ Sevinç, Kozlu Tolunay, Baþak Feyza (2017). The architecture of the lymph nodes in the abdominal and thoracic cavities of wild boar. Indian Journal of Animal Research. 53(5): 609-615. doi: 10.18805/ijar.B-759.

    ABSTRACT

    The distrubition of lymph nodes located in the abdominal and thoracic cavities of ten wild boars, and their structure were determined anatomically, histologically and immunohistochemically, to be the first detailed investigation on the wild boar. Though general localization and distribution were similar, the number of lymph nodes showed small differences from those of domestic pig. Histological investigations did not reveal a significant hilus. Besides, T lymphocytes with anti-CD3, CD4, CD8, B lymphocytes with anti-CD79a, macrophages with anti-macrophage monoclonal antibodies, and follicular dendritic cells using anti-S100 polyclonal antibody and their distrubution in the lymph nodes were detected. Many CD3 positive T lymphocytes were observed in the germinal center of the lymph follicles, in the cortical area and in the medulla. CD8 positive T lymphocytes were few, and CD4 positive T lymphocytes were not seen. CD79 positive cells were scanty.

    REFERENCES

    1. Bacha WJ and Bacha LM (2000). Color Atlas of Veterinary Histology. 2nd ed. Lippincott Williams & Wilkins. Philadelphia, 69-71. 
    2. Bancroft JD and Cook HC (1984). Manual of Histological Techniques. Churchill Livingstone. Edinburgh, London, Melbourne, and New York, 49-50, 83.
    3. Chianini F, Majo N, Segales J, Dominguez J and Domingo M (2001). Immunohistological study of the immune system cells in paraffin-embedded tissues of conventional pigs. Vet Immunol Immunopathol 82: 245-255. 
    4. Denk H, Künzele H, Plenk H, Rüschoff J and Sellner W (1989). Romeis Mikroskopische Technik. 17. Neubearbeitete Auflage. Urban und Schwarzenberg. München, 439-450.
    5. Dursun N (1999). Veteriner Anatomi II. Medisan Yayinevi. Ankara, 293-301.
    6. Dyce KM, Sack WO and Wensing CJG (2002). Textbook of Veterinary Anatomy. Saunders. Philedelphia. 
    7. Eurell JA and Frappier BL (2006). Dellmann’s Textbook of Veterinary Histology. 6th ed. Blackwell Publishing. Ames, Iowa, USA, 143-147. 
    8. Gutierrez M, Forster FI, McConnell SA, Cassidy JP, Pollock JM and Bryson DG (1999). The detection of CD2+, CD4+, CD8+, and WC1+ T lymphocytes, B cells and macrophages in fixed and paraffin embedded bovine tissue using a range of antigen recovery and signal amplification techniques. Vet Immunol Immunopathol 71: 321–334.
    9. Heizmann CW, Fritz G and Schafer BW (2002). S100 proteins: structure, functions and pathology. Front Biosci 1(7): 1356-1368.
    10. Hoshi N, Hashimoto Y, Kitagawa H, Kon Y and Kudo N (1986). Histological and immunohistochemical studies on the architecture of lymph nodes in pig. Jpn J Vet Sci 48(6): 1097-1107.
    11. Jonjic N, Jonjic S, Saalmüller A, Rukavina D and Koszinowski UH (1987). Distribution of T-lymphocyte subsets in porcine lymphoid tissues. Immunology 60: 395-401.
    12. Junqueira LC and Carneiro J (2006). Basic Histology. 10th ed. Translation: Y Aytekin and S Solakoðlu. Nobel Týp Kitabevleri. Ankara, 278-284.
    13. Kalita A, Kalita PC and Doley PJ (2014). Light microscopic study on the peripheral lymphnodes of mizo local pig (zo vawk). AJBPS 4(28): 4-8.
    14. Nomina Anatomica Veterinaria (NAV) (2012). International Committee on Veterinary Gross Anatomical Nomenclature and General Assembly of the World Association of Veterinary Anatomists. 5th ed. Published by the Editorial Committee Hannover (Germany), Columbia, MO (U.S.A.), Ghent (Belgium), Sapporo (Japan). 
    15. Ramos JA, Ramis AJ, Rabanal RM, Marco A, Domingo M and Ferrer LM (1990). Scaning electron microscopy of swine lymphoid organs. Histol Histopathol 5: 397-406.
    16. Saar LI and Getty R (1963). The lymph nodes and the lymph vessels of the abdominal wall, pelvic wall and the pelvic limb of swine. Iowa State University Veterinarian 26(2): 97-113.
    17. Sarsilmaz M, Gümüþalan Y, Çelik HH, Gezici M and Akþit MD (1994). Classification of the thoracic and abdominal lymph nodes of the rat. Turk J Med Res 12(b): 185-191.
    18. Schummer A, Wilkens H, Vollmerhaus B and Habermehl KH (1981). The Circulatory System, The Skin, and The Cutaneous Organs of The Domestic Mammals. Verlag Paul Parey. Hamburg.
    19. Spalding HJ and Heath TJ (1989). Fine structure of lymph pathways in nodes from the superficial inguinal lymph centre in the pig. J Anat 166: 43-54.
    20. Sugimura M, Shirogane D, Atoji Y, Suzuki Y, Ohshima Y, Kon Y and Hashimoto Y (1990). A comparative study on S-100 protein-    immunoreactive cells in lymph nodes. Jpn J Vet Sci 52(5): 1015-1021. 
    21. Tipirdamaz S (2000). Lenfatik Sistem. Sel-ün Vakfi Yayinlari. Konya, 57-82.
    22. Tingstedt JE, Tornehave D, Lind P and Nielsen J (2003). Immunohistochemical detection of SWC3, CD2, CD3, CD4 and CD8 antigens in paraformaldehyde fixed and paraffin embedded porcine lymphoid tissue. Vet Immunol Immunopathol 94: 123-132. 
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