Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 8 (august 2019) : 1080-1084,   Doi: 10.18805/ijar.B-737

Molecular characterization of universal Mycoplasma and Acholeplasma laidlawii from buffaloes (Bubalus bubalis) at Karachi, Pakistan.

S
Syed Khurram Fareed
F
Faiz Muhammad
J
Javed Muhammad
M
Masood Rabbani
T
Taseer Ahmed Khan
A
Allah Bux Kachiwal
S
Shakeel Ahmed Khan
A
Aqeel Ahmad
1Department of Microbiology, University of Karachi, Karachi, Pakistan.
Cite article:- Fareed Khurram Syed, Muhammad Faiz, Muhammad Javed, Rabbani Masood, Khan Ahmed Taseer, Kachiwal Bux Allah, Khan Ahmed Shakeel, Ahmad Aqeel (2018). Molecular characterization of universal Mycoplasma and Acholeplasma laidlawii from buffaloes (Bubalus bubalis) at Karachi, Pakistan.. Indian Journal of Animal Research. 53(8): 1080-1084. doi: 10.18805/ijar.B-737.

ABSTRACT

The present study was conducted to identify and characterize the bovine Acholeplasma laidlawii from 1120 clinically ill buffaloes and 470 lungs collected from buffaloes at abattoir of Karachi. All buffaloes were clinically examined and around 467 (13.48%) buffaloes had signs of respiratory distress including sticky nasal discharge, sunken eyes, dyspnea and fever. All the culture positive samples were further evaluated by PCR using specific primers of A. laidlawii and the amplified product to re-validate the sequence of the specie. This revealed that suspected buffaloes, 92 (2.66%) from nasal discharge and 44 (4.03%) from lung tissue samples respectively were positive for A. laidlawii. Rests of the samples were still under investigation. However it is first time proclaimed on research basis that A. laidlawii was infecting the buffaloes in Pakistan and precautionary measures should be taken to prevent losses from this specie in the months of October and April.  

REFERENCES

  1. Ahmad, Z., Babar, S., Abbas, F., Awan, M.A., Shafee, M. and Tariq, M.M., et al. (2014). Prevalence of Mycoplasma bovis in respiratory tract of cattle slaughtered in Balochistan, Pakistan. Pak. Vet. J. 34: 46-49.
  2. Allen, J.W., Viel, L., Bateman, K.G., Rosendal, S., Shewen, P.E. and Physick Sheard, P. (1991). The microbial flora of the respiratory tract in feedlot calves: associations between nasopharyngeal and bronchoalveolar lavage cultures. Can. J. Ve.t Res. 55: 341-346.
  3. Amin, S., Rahman, S., Hussain, I. and Muhammad, G. (2013). Seroprevalence of Mycoplasma ovipneumoniae among sheep from different districts of Baluchistan, Pakistan. Int. J. Agric. Biol. 15: 1043-1046.
  4. Arbuckle, J.B.R. and Bonson, M.D. (1979). The isolation of Acholeplasma oculi from an outbreak of ovine keratoconjuctivitis. Vet. Rec. 106: 15. 
  5. Awan, M.A., Abbas, F., Yasinzai, M., Nicholas, R.A., and Babar, S. et al. (2010). First report on the molecular prevalence of Mycoplasma capricolum subspecies capripneumoniae (Mccp) in goats the cause of contagious caprine pleuropneumonia (CCPP) in Balochistan province of Pakistan. Mol. Biol. Rep. 37: 3401-3406.
  6. Ayling, R.D., Bashiruddin, S.E. and Nicholas, R.A.J. (2004). Mycoplasma species and related organisms isolated from ruminants in Britain between 1990 and 2000. Vet. Rec. 155: 413–416. 
  7. Banerjee, M., Singh, N. and Gupta, P.P. (1979). Isolation of mycoplasmas and acholeplasmas from pneumonic lesions in sheep and goats in India. Zentralbl Veterinarmed B., 26: 689-95.
  8. Fareed, S.K., Memon, K.H., Kachiwal, A.B., Azhar, S., Brula, M.I., Hassan, M.U., Ali, M. and Khan, T.A. (2017). Prevalence and economic losses of reproductive disorders and mastitis in buffaloes at Karachi, Pakistan. Indian J. Anim. Res., 51(6) :1130-1133
  9. Fox, L.K., Kirk, J.H. and Britten, A. (2005). Mycoplasma mastitis: a review of transmission and control. J Vet Med B. 52: 153-60.
  10. Giovanni, A.M., Giulio, B., Franco, B. and Luciano, C. (1980). Colony morphology, ultrastructure and morphogenesis in mycoplasma hominis, Acholeplasma laidlawii and ureaplasma urea and ticum. J. Gen. Microbiol. 116: 435-443.
  11. Gonzalez, R.N. and Wilson, D.J. (2003). Mycoplasmal mastitis in dairy herds. Vet. Clin. North Am. Food. Anim. Pract. 19: 199–221.
  12. Hidetoshi, H., Hidetomo, I. and Hajime, N. (2011). A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle. ýJ. Vet. Sci. 12: 191-193. 
  13. Kulp, A. and Kuehn, M.J. (2010). Biological functions and biogenesis of secreted bacterial outer membrane vesicles. Annu. Rev. Microbiol. 64: 163–84.
  14. Mukhtar, M., Awais, M.M., Anwar, M.I., Hussain, Z., Bhatti, N. and Ali, S. (2012). Seroprevalence of Mycoplasma gallisepticum among commercial layers in Faisalabad, Pakistan. J. Basic Applied Sci. 8: 183-186.
  15. Roulland-Dussoix, D., Henry, A. and Lemercier, B. (1994). Detection of mycoplasmas in cell cultures by PCR. J. Microbiol. Methods. 19: 127-134.
  16. Sayyari, M. and Sharma, R. (2011). Pathological and bacteriological study of lobe distribution of lesions in buffalo (Bubalus bubalis). Int. J. of Vet. Res. 5: 125-128.
  17. Stipkovits, L., Ripley, P., Varga, J. and Palfi, V. (2000). Clinical study of the disease of calves associated with Mycoplasma bovis infection. Acta. Vet. Hung. 48: 387-395.
  18. Taoudi, A., Kirchhoff, H., Johnson, D.W., Choukrallah, A. (1985). Prevalence of mycoplasma and acholeplasma species in cattle exhibiting various clinical diseases and pathological lesions in Morocco. Zentralbl Veterinarmed B., 32: 534-40.
  19. Tenk, M. (2005). Examination of Mycoplasma bovis infection in cattle. PhD, Szent István University Postgraduate School of Veterinary Science, Budapest.
  20. Uemura, R., Sueyoshi, M. and Nagatomo, H. (2010). Antimicrobial susceptibilities of four species of mycoplasma isolated in 2008 and 2009 from cattle in Japan. J. Vet Med Sci. 72: 1661-1663.
  21. Van Kuppeveld, F.J., van der Logt, J.T., Angulo, A.F., van Zoest, M.J., Quint, W.G., Niesters, H.G., Galama, J.M. and Melchers, W.J. (1992). Genus and species-specific identification of mycoplasmas by 16S rRNA amplification. Appl. Environ. Microb. 58: 2606-2615.
  22. Vladislav, M.C., Alexey, A.M., Natalia, B.B, Elena, S.M, Tatiana, Y.G, Maxim, V.T, Innokentii, E.V., Anton, V.S., Sergei, N.B., Olga, A.C. (2014). Extracellular membrane vesicles secreted by mycoplasma Acholeplasma laidlawii PG8 are enriched in virulence proteins. J. of proteomics, 110: 17–128.
  23. Volokhov, D.V., Neverov, A.A., George, J., Kong, H., Liu, S.X., Anderson, C., Davidson, M.K. and Chizhikov, V. (2007). Genetic analysis of housekeeping genes of members of the genus Acholeplasma: phylogeny and complementary molecular markers to the 16S rRNA gene. Mol. Phylogenet. Evol. 44: 699-710.
  24. Wilson, D.J., Skirpstunas, R.T., Trujillo, J.D., Cavender, K.B., Bagley, C.V., Harding, R.L. (2007). Unusual history and initial clinical signs of Mycoplasma bovis mastitis and arthritis in first-lactation cows in a closed commercial dairy herd. J. Amer. Vet. Med. Assn. 230: 1519-23.
  25. Zafar, A., Shakeel, B., Ferhat, A., Muhammad, A.A., Muhammad, A., et al. (2011). Identification and molecular characterization of mycoplasma species from bovine lungs samples collected from slaughter house, Quetta, Balochistan, Pakistan. Pakistan J. Life. Soc. Sci. 9: 91-97. 
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 53 issue 8 (august 2019) : 1080-1084,   Doi: 10.18805/ijar.B-737

    Molecular characterization of universal Mycoplasma and Acholeplasma laidlawii from buffaloes (Bubalus bubalis) at Karachi, Pakistan.

    S
    Syed Khurram Fareed
    F
    Faiz Muhammad
    J
    Javed Muhammad
    M
    Masood Rabbani
    T
    Taseer Ahmed Khan
    A
    Allah Bux Kachiwal
    S
    Shakeel Ahmed Khan
    A
    Aqeel Ahmad
    1Department of Microbiology, University of Karachi, Karachi, Pakistan.
    Cite article:- Fareed Khurram Syed, Muhammad Faiz, Muhammad Javed, Rabbani Masood, Khan Ahmed Taseer, Kachiwal Bux Allah, Khan Ahmed Shakeel, Ahmad Aqeel (2018). Molecular characterization of universal Mycoplasma and Acholeplasma laidlawii from buffaloes (Bubalus bubalis) at Karachi, Pakistan.. Indian Journal of Animal Research. 53(8): 1080-1084. doi: 10.18805/ijar.B-737.

    ABSTRACT

    The present study was conducted to identify and characterize the bovine Acholeplasma laidlawii from 1120 clinically ill buffaloes and 470 lungs collected from buffaloes at abattoir of Karachi. All buffaloes were clinically examined and around 467 (13.48%) buffaloes had signs of respiratory distress including sticky nasal discharge, sunken eyes, dyspnea and fever. All the culture positive samples were further evaluated by PCR using specific primers of A. laidlawii and the amplified product to re-validate the sequence of the specie. This revealed that suspected buffaloes, 92 (2.66%) from nasal discharge and 44 (4.03%) from lung tissue samples respectively were positive for A. laidlawii. Rests of the samples were still under investigation. However it is first time proclaimed on research basis that A. laidlawii was infecting the buffaloes in Pakistan and precautionary measures should be taken to prevent losses from this specie in the months of October and April.  

    REFERENCES

    1. Ahmad, Z., Babar, S., Abbas, F., Awan, M.A., Shafee, M. and Tariq, M.M., et al. (2014). Prevalence of Mycoplasma bovis in respiratory tract of cattle slaughtered in Balochistan, Pakistan. Pak. Vet. J. 34: 46-49.
    2. Allen, J.W., Viel, L., Bateman, K.G., Rosendal, S., Shewen, P.E. and Physick Sheard, P. (1991). The microbial flora of the respiratory tract in feedlot calves: associations between nasopharyngeal and bronchoalveolar lavage cultures. Can. J. Ve.t Res. 55: 341-346.
    3. Amin, S., Rahman, S., Hussain, I. and Muhammad, G. (2013). Seroprevalence of Mycoplasma ovipneumoniae among sheep from different districts of Baluchistan, Pakistan. Int. J. Agric. Biol. 15: 1043-1046.
    4. Arbuckle, J.B.R. and Bonson, M.D. (1979). The isolation of Acholeplasma oculi from an outbreak of ovine keratoconjuctivitis. Vet. Rec. 106: 15. 
    5. Awan, M.A., Abbas, F., Yasinzai, M., Nicholas, R.A., and Babar, S. et al. (2010). First report on the molecular prevalence of Mycoplasma capricolum subspecies capripneumoniae (Mccp) in goats the cause of contagious caprine pleuropneumonia (CCPP) in Balochistan province of Pakistan. Mol. Biol. Rep. 37: 3401-3406.
    6. Ayling, R.D., Bashiruddin, S.E. and Nicholas, R.A.J. (2004). Mycoplasma species and related organisms isolated from ruminants in Britain between 1990 and 2000. Vet. Rec. 155: 413–416. 
    7. Banerjee, M., Singh, N. and Gupta, P.P. (1979). Isolation of mycoplasmas and acholeplasmas from pneumonic lesions in sheep and goats in India. Zentralbl Veterinarmed B., 26: 689-95.
    8. Fareed, S.K., Memon, K.H., Kachiwal, A.B., Azhar, S., Brula, M.I., Hassan, M.U., Ali, M. and Khan, T.A. (2017). Prevalence and economic losses of reproductive disorders and mastitis in buffaloes at Karachi, Pakistan. Indian J. Anim. Res., 51(6) :1130-1133
    9. Fox, L.K., Kirk, J.H. and Britten, A. (2005). Mycoplasma mastitis: a review of transmission and control. J Vet Med B. 52: 153-60.
    10. Giovanni, A.M., Giulio, B., Franco, B. and Luciano, C. (1980). Colony morphology, ultrastructure and morphogenesis in mycoplasma hominis, Acholeplasma laidlawii and ureaplasma urea and ticum. J. Gen. Microbiol. 116: 435-443.
    11. Gonzalez, R.N. and Wilson, D.J. (2003). Mycoplasmal mastitis in dairy herds. Vet. Clin. North Am. Food. Anim. Pract. 19: 199–221.
    12. Hidetoshi, H., Hidetomo, I. and Hajime, N. (2011). A simplified PCR assay for fast and easy mycoplasma mastitis screening in dairy cattle. ýJ. Vet. Sci. 12: 191-193. 
    13. Kulp, A. and Kuehn, M.J. (2010). Biological functions and biogenesis of secreted bacterial outer membrane vesicles. Annu. Rev. Microbiol. 64: 163–84.
    14. Mukhtar, M., Awais, M.M., Anwar, M.I., Hussain, Z., Bhatti, N. and Ali, S. (2012). Seroprevalence of Mycoplasma gallisepticum among commercial layers in Faisalabad, Pakistan. J. Basic Applied Sci. 8: 183-186.
    15. Roulland-Dussoix, D., Henry, A. and Lemercier, B. (1994). Detection of mycoplasmas in cell cultures by PCR. J. Microbiol. Methods. 19: 127-134.
    16. Sayyari, M. and Sharma, R. (2011). Pathological and bacteriological study of lobe distribution of lesions in buffalo (Bubalus bubalis). Int. J. of Vet. Res. 5: 125-128.
    17. Stipkovits, L., Ripley, P., Varga, J. and Palfi, V. (2000). Clinical study of the disease of calves associated with Mycoplasma bovis infection. Acta. Vet. Hung. 48: 387-395.
    18. Taoudi, A., Kirchhoff, H., Johnson, D.W., Choukrallah, A. (1985). Prevalence of mycoplasma and acholeplasma species in cattle exhibiting various clinical diseases and pathological lesions in Morocco. Zentralbl Veterinarmed B., 32: 534-40.
    19. Tenk, M. (2005). Examination of Mycoplasma bovis infection in cattle. PhD, Szent István University Postgraduate School of Veterinary Science, Budapest.
    20. Uemura, R., Sueyoshi, M. and Nagatomo, H. (2010). Antimicrobial susceptibilities of four species of mycoplasma isolated in 2008 and 2009 from cattle in Japan. J. Vet Med Sci. 72: 1661-1663.
    21. Van Kuppeveld, F.J., van der Logt, J.T., Angulo, A.F., van Zoest, M.J., Quint, W.G., Niesters, H.G., Galama, J.M. and Melchers, W.J. (1992). Genus and species-specific identification of mycoplasmas by 16S rRNA amplification. Appl. Environ. Microb. 58: 2606-2615.
    22. Vladislav, M.C., Alexey, A.M., Natalia, B.B, Elena, S.M, Tatiana, Y.G, Maxim, V.T, Innokentii, E.V., Anton, V.S., Sergei, N.B., Olga, A.C. (2014). Extracellular membrane vesicles secreted by mycoplasma Acholeplasma laidlawii PG8 are enriched in virulence proteins. J. of proteomics, 110: 17–128.
    23. Volokhov, D.V., Neverov, A.A., George, J., Kong, H., Liu, S.X., Anderson, C., Davidson, M.K. and Chizhikov, V. (2007). Genetic analysis of housekeeping genes of members of the genus Acholeplasma: phylogeny and complementary molecular markers to the 16S rRNA gene. Mol. Phylogenet. Evol. 44: 699-710.
    24. Wilson, D.J., Skirpstunas, R.T., Trujillo, J.D., Cavender, K.B., Bagley, C.V., Harding, R.L. (2007). Unusual history and initial clinical signs of Mycoplasma bovis mastitis and arthritis in first-lactation cows in a closed commercial dairy herd. J. Amer. Vet. Med. Assn. 230: 1519-23.
    25. Zafar, A., Shakeel, B., Ferhat, A., Muhammad, A.A., Muhammad, A., et al. (2011). Identification and molecular characterization of mycoplasma species from bovine lungs samples collected from slaughter house, Quetta, Balochistan, Pakistan. Pakistan J. Life. Soc. Sci. 9: 91-97. 
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)