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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 5 (may 2019) : 616-621,   Doi: 10.18805/ijar.B-730

Histological verification of seminiferous tubule structure injury a cause of asthenozoospermia in chicken

Q
Q. Guo
L
L. Xu
Y
Y. Bi
K
K. Zhang
S
S. Xu
Q
Q. Yuan
Y
Y. Xue
W
W. Lu
L
L. Ren
L
L. Qiu
Y
Y. Zhang
Q
Q. Xu
G
G. Chang
G
G. Chen
1Jiangsu Key Laboratory for Animal Genetics, Breeding and Molecular Design, Yangzhou University, Yangzhou-225 009, Jiangsu, China.
Cite article:- Guo Q., Xu L., Bi Y., Zhang K., Xu S., Yuan Q., Xue Y., Lu W., Ren L., Qiu L., Zhang Y., Xu Q., Chang G., Chen G. (2017). Histological verification of seminiferous tubule structure injury a cause of asthenozoospermia in chicken. Indian Journal of Animal Research. 53(5): 616-621. doi: 10.18805/ijar.B-730.

ABSTRACT

Asthenozoospermia puzzled poultry industry and little known about its characteristics. To explore the histological etiology of asthenozoospermia, we measured the sperm quality index (SQI) and Piwil1 expression in the testes of three types of asthenozoospermia roosters: field, artificial and normal. The SQI of field and artificial roosters was inferior to normal roosters. Further, busulfan could reduce sperm quality. Histological examination showed that the spermatids and spermatocyte of the seminiferous tubule was stripped layer-by-layer in field and artificial roosters. The absolute expression of Piwi11 in the normal group was significantly higher than that in the other two groups, and decreased with time. Piwi11 transcript expression rapidly decreased after 7 days post-injection (dpi); the lowest level was 13 dpi and did not change thereafter. Overall, our results indicate that busulfan causes structural damage to the seminiferous tubules, which can lead to asthenozoospermia, and that Piwil1 gene is a marker gene of asthenozoospermia.

REFERENCES

  1. Bamezai, S., Rawat V. P. and Buske C. (2012). Concise review: The Piwi-piRNA axis: pivotal beyond transposon silencing. Stem Cells 30(12): 2603-2611.
  2. Bi Y (2012). SNPs Analysis and mRNA Expression of Candidate Genes associated with Semen Quality in Beijing-You Chicken. Master’s, Yangzhou University.
  3. Bublat, A., Fischer, D. and Bruslund S. (2017). Seasonal and genera-specific variations in semen availability and semen characteristics in large parrots. Theriogenology 91: 82-89.
  4. Carmell, M. A., A. Girard, and H. J. G. van de Kant,. (2007). MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Developmental Cell 12(4): 503-514.
  5. Deng, W. and H. Lin (2002). miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis. Dev Cell 2(6): 819-830.
  6. Ferreira, H. J., Heyn, H.. and Garcia del Muro X.. (2014). Epigenetic loss of the PIWI/piRNA machinery in human testicular tumorigenesis. Epigenetics 9(1): 113-118.
  7. Kuramochi-Miyagawa, S., T. Kimura, and T. W. Ijiri (2004). Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development 131(4): 839-849.
  8. Lake P (1971). In physiology and biochemistry of the domestic fowl III. The Male in Reproduction 11: 1411-1415.
  9. Liu X, Wen J and L. J (2004). Study sperm density and source intracytoplasmic sperm injection results Influence. Medical Journal of Liaoning 01: 4-6.
  10. Love, C. C. (2011). Relationship between sperm motility, morphology and the fertility of stallions. Theriogenology 76(3): 547-557.
  11. Mehrabani, D., Hassanshahi M.A., and Tamadon A. (2015). Adipose tissue-derived mesenchymal stem cells repair germinal cells of seminiferous tubules of busulfan-induced azoospermic rats. J Hum Reprod Sci 8(2): 103-110.
  12. Panahi, M., Keshavarz S., and Rahmanifar F. (2015). Busulfan induced azoospermia: Stereological evaluation of testes in rat. Vet Res Forum 6(4): 273-278.
  13. Parker J, Mckenzie F and K. H (1942). Fertility in the male domestic fowl. Missouri Agricultural Experiment Station Research Bulletin 22(5): 347-353.
  14. Ren HY, Zhong R, Ding XP, Chen ZY, Jing YL. Investigation of polymorphisms in exon7 of the NSUN7 gene among Chinese Han men with asthenospermia. Genetics and Molecular Research 2015;14:9261-8.
  15. Shi H (1998). An overvie of combined traditional and western medical treatment on asthenozoospermia and oligozoospermia. The Chinese Journal of Human Sexuality 16(18): 40.
  16. Skurikhin, E. G., Pakhomova A. V.,and Pershina O. V. (2017). Regenerative Potential of Stem and Progenitor Cells from Ischemic Testes of C57Bl/6 Mice in Culture and in the Model of Spermatogenesis Suppression Caused by Busulfan. Bull Exp Biol Med 162(3):400-405.
  17. Williams C (1956). An analysis of the peck-order of the female domestic fowl gallusdomesticus. Poultry Science 35(9): 969-976.
  18. Wu X, Ma C and W. H (2010). Asthenozoospermia etiology and treatment of traditional Chinese medicine and Western medicine. Modern Journal of Integrated Traditional Chinese and Western Medicine 30: 3357-3360.
  19. Xu, L., Chang G., and Ma T. (2016). Piwil1 mediates meiosis during spermatogenesis in chicken. Anim Reprod Sci 166: 99-108.
  20. Zhai F, Wang H, and Xu L (2014). Preliminary Study on Factors in Malesasthenozoospermia of Cocks. Chinese Journal of Animal Science 17: 30-32+96. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 5 (may 2019) : 616-621,   Doi: 10.18805/ijar.B-730

    Histological verification of seminiferous tubule structure injury a cause of asthenozoospermia in chicken

    Q
    Q. Guo
    L
    L. Xu
    Y
    Y. Bi
    K
    K. Zhang
    S
    S. Xu
    Q
    Q. Yuan
    Y
    Y. Xue
    W
    W. Lu
    L
    L. Ren
    L
    L. Qiu
    Y
    Y. Zhang
    Q
    Q. Xu
    G
    G. Chang
    G
    G. Chen
    1Jiangsu Key Laboratory for Animal Genetics, Breeding and Molecular Design, Yangzhou University, Yangzhou-225 009, Jiangsu, China.
    Cite article:- Guo Q., Xu L., Bi Y., Zhang K., Xu S., Yuan Q., Xue Y., Lu W., Ren L., Qiu L., Zhang Y., Xu Q., Chang G., Chen G. (2017). Histological verification of seminiferous tubule structure injury a cause of asthenozoospermia in chicken. Indian Journal of Animal Research. 53(5): 616-621. doi: 10.18805/ijar.B-730.

    ABSTRACT

    Asthenozoospermia puzzled poultry industry and little known about its characteristics. To explore the histological etiology of asthenozoospermia, we measured the sperm quality index (SQI) and Piwil1 expression in the testes of three types of asthenozoospermia roosters: field, artificial and normal. The SQI of field and artificial roosters was inferior to normal roosters. Further, busulfan could reduce sperm quality. Histological examination showed that the spermatids and spermatocyte of the seminiferous tubule was stripped layer-by-layer in field and artificial roosters. The absolute expression of Piwi11 in the normal group was significantly higher than that in the other two groups, and decreased with time. Piwi11 transcript expression rapidly decreased after 7 days post-injection (dpi); the lowest level was 13 dpi and did not change thereafter. Overall, our results indicate that busulfan causes structural damage to the seminiferous tubules, which can lead to asthenozoospermia, and that Piwil1 gene is a marker gene of asthenozoospermia.

    REFERENCES

    1. Bamezai, S., Rawat V. P. and Buske C. (2012). Concise review: The Piwi-piRNA axis: pivotal beyond transposon silencing. Stem Cells 30(12): 2603-2611.
    2. Bi Y (2012). SNPs Analysis and mRNA Expression of Candidate Genes associated with Semen Quality in Beijing-You Chicken. Master’s, Yangzhou University.
    3. Bublat, A., Fischer, D. and Bruslund S. (2017). Seasonal and genera-specific variations in semen availability and semen characteristics in large parrots. Theriogenology 91: 82-89.
    4. Carmell, M. A., A. Girard, and H. J. G. van de Kant,. (2007). MIWI2 is essential for spermatogenesis and repression of transposons in the mouse male germline. Developmental Cell 12(4): 503-514.
    5. Deng, W. and H. Lin (2002). miwi, a murine homolog of piwi, encodes a cytoplasmic protein essential for spermatogenesis. Dev Cell 2(6): 819-830.
    6. Ferreira, H. J., Heyn, H.. and Garcia del Muro X.. (2014). Epigenetic loss of the PIWI/piRNA machinery in human testicular tumorigenesis. Epigenetics 9(1): 113-118.
    7. Kuramochi-Miyagawa, S., T. Kimura, and T. W. Ijiri (2004). Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development 131(4): 839-849.
    8. Lake P (1971). In physiology and biochemistry of the domestic fowl III. The Male in Reproduction 11: 1411-1415.
    9. Liu X, Wen J and L. J (2004). Study sperm density and source intracytoplasmic sperm injection results Influence. Medical Journal of Liaoning 01: 4-6.
    10. Love, C. C. (2011). Relationship between sperm motility, morphology and the fertility of stallions. Theriogenology 76(3): 547-557.
    11. Mehrabani, D., Hassanshahi M.A., and Tamadon A. (2015). Adipose tissue-derived mesenchymal stem cells repair germinal cells of seminiferous tubules of busulfan-induced azoospermic rats. J Hum Reprod Sci 8(2): 103-110.
    12. Panahi, M., Keshavarz S., and Rahmanifar F. (2015). Busulfan induced azoospermia: Stereological evaluation of testes in rat. Vet Res Forum 6(4): 273-278.
    13. Parker J, Mckenzie F and K. H (1942). Fertility in the male domestic fowl. Missouri Agricultural Experiment Station Research Bulletin 22(5): 347-353.
    14. Ren HY, Zhong R, Ding XP, Chen ZY, Jing YL. Investigation of polymorphisms in exon7 of the NSUN7 gene among Chinese Han men with asthenospermia. Genetics and Molecular Research 2015;14:9261-8.
    15. Shi H (1998). An overvie of combined traditional and western medical treatment on asthenozoospermia and oligozoospermia. The Chinese Journal of Human Sexuality 16(18): 40.
    16. Skurikhin, E. G., Pakhomova A. V.,and Pershina O. V. (2017). Regenerative Potential of Stem and Progenitor Cells from Ischemic Testes of C57Bl/6 Mice in Culture and in the Model of Spermatogenesis Suppression Caused by Busulfan. Bull Exp Biol Med 162(3):400-405.
    17. Williams C (1956). An analysis of the peck-order of the female domestic fowl gallusdomesticus. Poultry Science 35(9): 969-976.
    18. Wu X, Ma C and W. H (2010). Asthenozoospermia etiology and treatment of traditional Chinese medicine and Western medicine. Modern Journal of Integrated Traditional Chinese and Western Medicine 30: 3357-3360.
    19. Xu, L., Chang G., and Ma T. (2016). Piwil1 mediates meiosis during spermatogenesis in chicken. Anim Reprod Sci 166: 99-108.
    20. Zhai F, Wang H, and Xu L (2014). Preliminary Study on Factors in Malesasthenozoospermia of Cocks. Chinese Journal of Animal Science 17: 30-32+96. 
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