Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 10 (october 2018) : 1395-1399,   Doi: 10.18805/ijar.B-702

The Interleukin-8 gene polymorphism and its association with milk production traits in holstein cows
 

A
Aswah Ridhowi
H
Hsiu-Luan Chang
C
Chia-Lin Liang
S
Suyadi
M
Ming-Che Wu
1Faculty of Animal Husbandry, University of Brawijaya. Jl. Veteran, Malang 65145. Jawa Timur. Indonesia
Cite article:- Ridhowi Aswah, Chang Hsiu-Luan, Liang Chia-Lin, Suyadi, Wu Ming-Che (2017). The Interleukin-8 gene polymorphism and its association with milk production traits in holstein cows. Indian Journal of Animal Research. 52(10): 1395-1399. doi: 10.18805/ijar.B-702.

ABSTRACT

This study proposed to identify a polymorphism in the IL8 gene in Holstein cows and analyze the relationship with milk production traits in Taiwanese Holstein cows. Investigations were done for single nucleotide polymorphism at position 2862 and the impact of IL8-T2862C polymorphism on the milk production traits of 68 Holstein cows was randomly analyzed by fixed effects model. No significant difference was found between genotypes for 305-2X-ME, fat%, protein%, total solid%, and somatic cell counts score (SCS). Significanteffects were observed on daily milk yield (MY), SCC (p<0.05), and lactose% (p<0.01). Cows with the CC genotype had less SCC compared with CT and TT ones. The allelic substitution effect was 0.70 kg daily milk yield for IL8 favorable allele T (p<0.05), and 3.24 x 104 cells/mL SCC for IL8favorable allele C (p<0.01). Significant dominance effects of IL8-T2862C on daily milk yield (1.22 kg,p<0.05) and lactose% (+0.07%, p<0.01) were found. 

REFERENCES

  1. Bhupal, G. (2007). Cytokine expression in milk somatic cells during mastitis in cattle and buffaloes. Ms. Thesis. Anand Agricultural University, Anand, Gujarat. India.
  2. Boutinaud, M., J. G. Flament, and H.Jammes. (2004). The number and activity of mammary epithelial cells,determining factors for milk production.Reprod. Nutr. Dev. 44:499-508.
  3. Bytyqi, H., U. Zaugg, K. Sherififi, A. Hamidi, M. Gjonbalaj, S. Muji, and H. Mehmeti. (2010). Influence of management and physiological factors on somatic cell count in raw cow milk in Kosova. Vet. Arhiv.80:173-183.
  4. Chen, R., Z. Yang, D. Ji, Y. Mao, Y. Chen, Y. Li, H. Wu, X. Wang, and L. Chang. (2011). Polymorphisms of IL8 gene correlate with milking traits, SCS and mRNA level in Chinese Holstein. Mol. Bio. Rep. 38:4083-4088.­­
  5. Dekkers, J. C. M., and F. Hospital. (2001). The use of molecular genetics in improvement of agricultural populations. Nat. Rev. Genet. 3:22-32.
  6. Fonseca, I., G. R. Antunes, D. S. Paiva, C. C. Lange, S. E. F. Guimares, and M. F. Martins. (2011). Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. Gen. Mol. Res.10:1295-1303.
  7. Hunt, K. M., J. E. Williams, B. Shafii, M. K. Hunt, R. Behre, R. Ting, M. K. McGuire, and M. A. McGuire. (2012). Mastitis is associated with increased free fatty acids, somatic cell count, and interleukin-8 concentrations in human milk. Breastfeed. Med. 8:105-110.
  8. Leitner, G., R. Eligulashvily, O. Krifucks, S. Perl, and A. Saran. (2002). Immune cell differentiation in mammary gland tissues and milk of cows chronically infected with Staphylococcus aureus. J. Vet. Med. 50:45-52.
  9. Mahesh. S. D., G. R. Gowane, L. S. Kokate, S. B. Kumar, and B. Bhushan. (2011). CXCR gene and its association with mastitis resistance in cattle-a Review.Int. J. Stress Manage. 2:444-447.
  10. Nishimura, T. (2003). Expression of potential lymphocyte trafficking mediator molecules in the mammary gland.Vet. Res.34:3-10.
  11. Paape, M. J., K. Shafer-Weaver, A. V. Capuco, K. Van-Oostveldt, and C. Burvenich.(2000). Immune surveillance of mammary tissue by phagocytic cells. Adv. Exp. Med. Biol.480:259-277.
  12. Proudfoot, A. I. (2002). Chemokine receptors: multifaceted therapeutic targets. Nat. Rev. Immunol.2:106-115.
  13. Rainard, P., and C. Riollet. (2006). Innate immunity of the bovine mammary gland. Vet. Res.37:369-400.
  14. Rambeaud, M., R. Clift, and G.M. Pighetti. (2006). Association of a bovine CXCR2 gene polymorphism with neutrophil survival and killing ability. J. Vet. Immun.111:231-238.
  15. Ruegg, P. L. (2001). Milk secretion and quality standards.The Babcock Institute University of Wisconsin, Madison, USA.
  16. Sharif, A., and G. Muhammad. (2008). Somatic cell count as an indicator of udder health status under modern dairy production: a review. Pakistan Vet. J.28:194-200.
  17. Shuster, D. E., E. K. Lee, and M. E. Kehrli Jr. (1996). Bacterial growth, inflammatory cytokine production, and neutrophil recruitment during coliform mastitis in cows within ten days after calving, compared with cows at midlactation. J. Vet. Res.57:1569-    1575.
  18. Youngerman, S. M., A. M. Saxton, S. P. Oliver, and G. M. Pighetti. (2004). Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle. J. Dairy Sci.87:2442-2448.
  19. Zhang, W., and H. Chen. (2002). The study on the interleukin-8 (IL8). Sheng Wu Yi Xue Gong Cheng XueZaZhi.9:697-702.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 52 issue 10 (october 2018) : 1395-1399,   Doi: 10.18805/ijar.B-702

    The Interleukin-8 gene polymorphism and its association with milk production traits in holstein cows
     

    A
    Aswah Ridhowi
    H
    Hsiu-Luan Chang
    C
    Chia-Lin Liang
    S
    Suyadi
    M
    Ming-Che Wu
    1Faculty of Animal Husbandry, University of Brawijaya. Jl. Veteran, Malang 65145. Jawa Timur. Indonesia
    Cite article:- Ridhowi Aswah, Chang Hsiu-Luan, Liang Chia-Lin, Suyadi, Wu Ming-Che (2017). The Interleukin-8 gene polymorphism and its association with milk production traits in holstein cows. Indian Journal of Animal Research. 52(10): 1395-1399. doi: 10.18805/ijar.B-702.

    ABSTRACT

    This study proposed to identify a polymorphism in the IL8 gene in Holstein cows and analyze the relationship with milk production traits in Taiwanese Holstein cows. Investigations were done for single nucleotide polymorphism at position 2862 and the impact of IL8-T2862C polymorphism on the milk production traits of 68 Holstein cows was randomly analyzed by fixed effects model. No significant difference was found between genotypes for 305-2X-ME, fat%, protein%, total solid%, and somatic cell counts score (SCS). Significanteffects were observed on daily milk yield (MY), SCC (p<0.05), and lactose% (p<0.01). Cows with the CC genotype had less SCC compared with CT and TT ones. The allelic substitution effect was 0.70 kg daily milk yield for IL8 favorable allele T (p<0.05), and 3.24 x 104 cells/mL SCC for IL8favorable allele C (p<0.01). Significant dominance effects of IL8-T2862C on daily milk yield (1.22 kg,p<0.05) and lactose% (+0.07%, p<0.01) were found. 

    REFERENCES

    1. Bhupal, G. (2007). Cytokine expression in milk somatic cells during mastitis in cattle and buffaloes. Ms. Thesis. Anand Agricultural University, Anand, Gujarat. India.
    2. Boutinaud, M., J. G. Flament, and H.Jammes. (2004). The number and activity of mammary epithelial cells,determining factors for milk production.Reprod. Nutr. Dev. 44:499-508.
    3. Bytyqi, H., U. Zaugg, K. Sherififi, A. Hamidi, M. Gjonbalaj, S. Muji, and H. Mehmeti. (2010). Influence of management and physiological factors on somatic cell count in raw cow milk in Kosova. Vet. Arhiv.80:173-183.
    4. Chen, R., Z. Yang, D. Ji, Y. Mao, Y. Chen, Y. Li, H. Wu, X. Wang, and L. Chang. (2011). Polymorphisms of IL8 gene correlate with milking traits, SCS and mRNA level in Chinese Holstein. Mol. Bio. Rep. 38:4083-4088.­­
    5. Dekkers, J. C. M., and F. Hospital. (2001). The use of molecular genetics in improvement of agricultural populations. Nat. Rev. Genet. 3:22-32.
    6. Fonseca, I., G. R. Antunes, D. S. Paiva, C. C. Lange, S. E. F. Guimares, and M. F. Martins. (2011). Differential expression of genes during mastitis in Holstein-Zebu crossbreed dairy cows. Gen. Mol. Res.10:1295-1303.
    7. Hunt, K. M., J. E. Williams, B. Shafii, M. K. Hunt, R. Behre, R. Ting, M. K. McGuire, and M. A. McGuire. (2012). Mastitis is associated with increased free fatty acids, somatic cell count, and interleukin-8 concentrations in human milk. Breastfeed. Med. 8:105-110.
    8. Leitner, G., R. Eligulashvily, O. Krifucks, S. Perl, and A. Saran. (2002). Immune cell differentiation in mammary gland tissues and milk of cows chronically infected with Staphylococcus aureus. J. Vet. Med. 50:45-52.
    9. Mahesh. S. D., G. R. Gowane, L. S. Kokate, S. B. Kumar, and B. Bhushan. (2011). CXCR gene and its association with mastitis resistance in cattle-a Review.Int. J. Stress Manage. 2:444-447.
    10. Nishimura, T. (2003). Expression of potential lymphocyte trafficking mediator molecules in the mammary gland.Vet. Res.34:3-10.
    11. Paape, M. J., K. Shafer-Weaver, A. V. Capuco, K. Van-Oostveldt, and C. Burvenich.(2000). Immune surveillance of mammary tissue by phagocytic cells. Adv. Exp. Med. Biol.480:259-277.
    12. Proudfoot, A. I. (2002). Chemokine receptors: multifaceted therapeutic targets. Nat. Rev. Immunol.2:106-115.
    13. Rainard, P., and C. Riollet. (2006). Innate immunity of the bovine mammary gland. Vet. Res.37:369-400.
    14. Rambeaud, M., R. Clift, and G.M. Pighetti. (2006). Association of a bovine CXCR2 gene polymorphism with neutrophil survival and killing ability. J. Vet. Immun.111:231-238.
    15. Ruegg, P. L. (2001). Milk secretion and quality standards.The Babcock Institute University of Wisconsin, Madison, USA.
    16. Sharif, A., and G. Muhammad. (2008). Somatic cell count as an indicator of udder health status under modern dairy production: a review. Pakistan Vet. J.28:194-200.
    17. Shuster, D. E., E. K. Lee, and M. E. Kehrli Jr. (1996). Bacterial growth, inflammatory cytokine production, and neutrophil recruitment during coliform mastitis in cows within ten days after calving, compared with cows at midlactation. J. Vet. Res.57:1569-    1575.
    18. Youngerman, S. M., A. M. Saxton, S. P. Oliver, and G. M. Pighetti. (2004). Association of CXCR2 polymorphisms with subclinical and clinical mastitis in dairy cattle. J. Dairy Sci.87:2442-2448.
    19. Zhang, W., and H. Chen. (2002). The study on the interleukin-8 (IL8). Sheng Wu Yi Xue Gong Cheng XueZaZhi.9:697-702.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)