Molecular identification studies  on Oestrus ovis L. (Diptera:Oestridae) larvae infested sheep in jazan region , Saudi Arabia

DOI: 10.18805/ijar.v0iOF.8487    | Article Id: B-681 | Page : 105-110
Citation :- Molecular identification studies on Oestrus ovis L. (Diptera:Oestridae) larvae infested sheep in jazan region , Saudi Arabia .Indian Journal Of Animal Research.2018.(52):105-110

Bosly . A. Hanan

dr_2009_bosly@hotmail.com
Address :

Biology Department, Faculty of Science, Jazan University, Jazan, Saudia Arabia

Submitted Date : 31-12-2016
Accepted Date : 11-04-2017

Abstract

Oestrus ovis L. (O. ovis) (Diptera: Oestridae) (Sheep Bot Fly) is ubiquitous in distribution. Myiasis causing larvae collected from Abu-Arish area (Eastern Jazan), Saudi Arabia were confirmed as O. ovis based on morphological traits. Polymerase chain reaction (PCR) studies targeting amplification of partial fragment (606 bp) of mitochondrial cytochrome oxidase subunitI (mtCOI) gene further confirmed the species. Phylogenetic analysis based on the partial (mtCOI) gene sequence revealed that the accession KU921431 showed 97% similarity based on nucleotide pairs of O.ovis accessions, retrieved from the GenBank. Thus, a method of molecular identification of O.ovis larvae was established as a credible substitution to morphological identification in Jazan region, Saudi Arabia.

Keywords

mtCOI Oestrus ovis PCR Phylogeny and Sequence.

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