Accacia senegel extract ameliorates Lead Acetate-mediated Hepatotoxicity in rats

DOI: 10.18805/ijar.v0iOF.7998    | Article Id: B-632 | Page : 1425-1430
Citation :- Accacia senegel extract ameliorates Lead Acetate-mediated Hepatotoxicity in rats.Indian Journal Of Animal Research.2019.(53):1425-1430
H.F. Gomaa, Fatimah A. Al Homaid and Ali Ismaiel Ali Abdel Rhaim shery-yahia2009@hotmail.com
Address : Zoology Department, Faculty of Science, Ain Shams University, Egypt. 
Submitted Date : 1-10-2016
Accepted Date : 11-01-2017

Abstract

Lead is a toxic metal that induces a wide range of physiological and histological changes inhuman and animals. Oxidative damage has been the possible mechanism involved in lead toxicity. The current study was carried out to evaluate the antioxidant activities of Accacia senegel extract against leadacetate-induced hepatic injury in rats. Four groups of rats were used in this study, Control, Lead acetate(8 mg/kg body weight intraperitoneally), Accacia senegel (7.5g/kg body weight /day orally) and Accacia senegel (7.5g/kg body weight /day orally)followed by lead acetate (8 mg/kg body weight intraperitoneally) respectively.All groups received the oral treatment by stomach tube once daily for 4 weeks. Lead intoxication resulted in a significant increase inserum alanine transase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP)activities,serum total cholesterol and triglycerides liver tumor necrosis factor-a (TNF-a), caspase-3, malondialdehyde (MDA), nitric oxide (NO) levels and a significantdecline of serum total proteins, liver reduced glutathione (GSH)  level and catalase. The hepatocytes showed degeneration with vacuolated cytoplasm and fibrosis. The administration ofAccacia senegel extractshowed a slight improving in the activities of Catalase, a slight decreasein AST, ALP activities, NO, Triglycerides but resulted in more increase in the levels of Total proteins, MDA, TNF-a and activity of Caspase-3 while did not induce any protection against elevated levels of ALT and Total cholesterol and against damage in hepatocytes including fibrosis and apoptosis.

Keywords

Lead acetate Accacia senegel Lipid peroxidation Apoptosis

References

  1. Abdel Moneim A. E. (2016). Indigofera oblongifolia prevents lead acetate-induced hepatotoxicity, oxidative stress, fibrosis and apoptosis in rats. Biol Trace Elem Res. 173(2):354-361. 
  2. Abdou H. M, and Newairy A. A. (2006). Hepatic and Reproductive Toxicity of Lead in Female Rats and Attenuation by Flaxseed Lignans. JMRI. 27 (4): 295-302.
  3. Al-Kenanny E. R.; Al-Hayaly L. K. and Al-Badrany A. G. (2012). Protective Effect of Arabic Gum on liver Injury Experimentally Induced by Gentamycin in Mice Kufa. Journal For Veterinary Medical Sciences. 3 (1):27-32. 
  4. Attia A. M. M.; Ibrahim F. A. A.; Nabil G. M. and Aziz S. W. (2013). Antioxidant effects of ginger (Zingiber officinale Roscoe) against lead acetate-induced hepatotoxicity in rats. African Journal of Pharmacy and Pharmacology. 7(20): 1213-1219.
  5. Aziz F.M. (2012). Protective Effects of Latex of Ficus carica L. against Lead Acetate-Induced Hepatotoxicity in Rats. Jordan Journal of Biological Sciences. 5(3):175-182.
  6. Bharali M. Kr. (2013). Effect of acute lead acetate exposure on liver of mice. Journal of Global Biosciences. 2(5): 121-125.
  7. Elderbi M. A; Mohamed A.H.; Hadi A.A. and Dabobash M. D.(2014). Potential protective effect of Gum Arabic against Doxorubicin–    induced cardiotoxicity in wistar albino rats. IJPSR. 5 (3):1023-1027. 
  8. Ellman GL. (1959).Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82(1):70-77.
  9. El-Menoufy G. A. M. (2012). Bee honey dose-dependently ameliorates lead acetate- mediated hepatorenal toxicity in rats. Life Science Journal. 9(4):780-788.
  10. El-Nekeety A., El-Kady A., Soliman M.S. and Abdel –Wahhab M.A. (2009). Protective effect of Aquilega vulgaris (L.). against lead acetate-induced oxidative stress in rats. Food and Chemical Toxicology. 47(9):2209-2215.
  11. Elshama S. S.; El-Kenawy A.E.; Osman H. H . and Youseef H. M. (2014). Amelioration of indomethacin systemic toxicity by gum arabic administration in adult albino rats. International Journal of Medicinal. Plants and Alternative Medicine. 2(3): 032-046. 
  12. El-Tantawy W.H.(2016). Antioxidant effects of Spirulina supplement against lead acetate-induced hepatic injury in rats. Journal of Traditional and Complementary Medicine. Journal of Traditional and Complementary Medicine. 6(4): 327–331.
  13. Ghosh D.; Dey M.; Ghosh A. K.; Chattopadhyay A. and Bandyopadhyay D. (2014). Melatonin protects against lead acetate-induced changes in blood corpuscles and lipid profile of male Wistar rats. Journal of Pharmacy Research. 8(3):336-342.
  14. Haleagrahara N.; Jackie T.;. Chakravarthi S; M. Rao and. Kulur A. (2010). Protective effect of Etlingera elatior (torch ginger) extract on lead acetate-induced hepatotoxicity in rats. J. Toxicol. Sci. 35: 663–671.
  15. Heba M. Abdou and Mohamed A. Hassan. (2014). Protective role of omega-3 polyunsaturated fatty acid against lead acetate-induced toxicity in liver and kidney of female rats. BioMed Research International. 1-11.
  16. Jarad A. S. (2012). Protective effect of Garlic against lead acetate toxicity in some biochemical and histopathological parameters in rats. Al-Anbar J. Vet. Sci. 5 (1): 1999-6527.
  17. Khan N.; Naqvi A.; Perveen K.A. and Rafique M. (2008). Lead induced nephrotoxicity with special reference to proximal in albino rats. Pakistan Journal of Pharmacology. 25(1): 29-35.
  18. Kumar P.V.; bricey A.A., Selvi V.V., kumar C. S. and Ramesh N. (2010). Biopotency of Allium sativum. Linn (Garlic) in Lead Acetate Intoxicated Male Albino Rats. Der Pharmacia Sinica. 1 (2): 1-4.
  19. Lowry O.H.; Rosebrough N.J.; Farr A.L. and Randall RJ. (1951). Protein measurement with the Folin phenol reagent. J. Biol. Chem.,    193:265-275.
  20. Ohkawa H, Ohishi N, Yagi K. (1979). Assay of lipid peroxide in animal tissue by thiobarbituric acid reaction. Anal. Biochem.,Vol. 95(2): 351-358.
  21. Omaima N. (2013). Renal and extrarenal effects of gum arabic (Acacia Senegal) – What can be learned from animal induced experiments? Kidney Blood Press Res. 37: 269-279.
  22. Pal R.; Hooda M. S.; Bias C.S.; Singh J.(2014). Hepatoprotective activity of acacia senegal pod against carbon tetrachloride-hepatotoxicity in rats. Int. J. Pharm. Sci. Rev. Res. 26(1): 165-168.
  23. Ponce-Canchihuamán J. C, Pérez-Méndez O.; Hernández-Muñoz R.; Torres-Durán P. V.and Juárez-Oropeza M. A.(2010). Protective effects of Spirulina maxima on hyperlipidemia and oxidative-stress induced by lead acetate in the liver and kidney. Lipids in Health and Disease, 9:35-42.
  24. Sivaprasad R; Nagaraj M and Varalakshmi P. (2004). Combined efficacies of lipoic acid and 2, 3-dimercaptosuccinic acid against lead-induced lipid peroxidation in rat liver. J Nutr. Biochem., 15:18–23.
  25. Turner, J.R. (2001). Introduction to analysis of variance: Design, analysis & interpretation. sage publication., thousand oaks, CA.
  26. Wang J.; ZhuH.; Yang Z. and Liu Z. (2013). Antioxidative effects of hesperetin against lead acetate-induced oxidative stress in rats. Indian J. Pharmacol. 45(4): 395–398.
  27. Yu J.; Fujishiro H.; Miyataka H.; Oyama T. M.; Hasegawa T. Seko Y; Miura N. and Himeno S. (2009). Dichotomous effects of lead acetate on the expression of metallothionein in the liver and kidney of mice. Biol. Pharm. Bull. 32(6): 1037—1042. 

Global Footprints