Banner
Current IssueEditorial BoardOnline First Articles
Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Research Article

Indian Journal of Animal Research, volume 53 issue 12 (december 2019) : 1666-1670

Study on the anti bovine viral diarrhea virus effect of swainsonine extract of Astragalus strictus Grah Ex Bend in vitro

Baocheng Hao, Xiaoyong Xing, Fanling Wu, Haitao Xiang, Fenqin Wen, Yonghao Hu, Jianping Liang
1<div style="text-align: justify;">College of Veterinary Medicine,&nbsp;Gansu Agricultural University, Lanzhou 730070,PR China.</div>
Cite article:- Hao Baocheng, Xing Xiaoyong, Wu Fanling, Xiang Haitao, Wen Fenqin, Hu Yonghao, Liang Jianping (2016). Study on the anti bovine viral diarrhea virus effect of swainsonine extract of Astragalus strictus Grah Ex Bend in vitro. Indian Journal of Animal Research. 53(12): 1666-1670. doi: 10.18805/ijar.v0iOF.6832.

ABSTRACT

This study was designed to investigate anti-virus action of the swainsonine (SW) extract of Astragalus strictus Grah. Ex Bend on bovine viral diarrhea virus (BVDV). Using cell culture techniques, and combining cytopathic effect (CPE) observation method and methyl thiazolyl tetrazolium (MTT) method to detect the toxicities of different concentrations of SW in bovine kidney primary cells (Madin-Darby Bovine Kidney Cells, MDBK), determine the drug safety concentration and TD50, Testing the comprehensive effect of different concentrations of SW for BVDV by adding swainsonine after virus-and-swainsonine added. The results showed that the TCID50 of BVDV is 10-4.7; there is no toxicity to MDBK when the concentration of SW below 0.256,mg·mL-1, and TD50 is 2.512mg·mL-1; Under the way of comprehensive action, IC50 is 0.782mg·mL-1, TI is 3.21. it showed that the comprehensive effect on BVDV of SW (65.29%, P<0.01) is good. The anti-BVDV effect of swainsonine in vitro is good and the antiviral mechanism of SW was speculated to SW inhibit the replication of BVDV proliferation or directly inactivate free BVDV.

REFERENCES

  1. Chen, J.P., Zhao, B.Y., Lu, H., Xu, C.M., Ma, Y. and Zhou, Q.W. (2013). The fermentation condition optimization of swainsonine-producing endophyte Aspergillus ustus. China Journal of Veterinary Sciences. 33:423-427. 
  2. Cui, J.G. (2005). Studies on the Antibacterial, Antiviral Effect and Immunopharmacology of Solanum Lyratum Thunb Alkaloids. Changsha: Hunan Agricultural University Master Thesis., 35-39.
  3. Goss, P.E., Reid, C.L., Bailey, D. and Dennis, J.W. (1997). Phase IB clinical trial of the oligosaccharide processing inhibitor swainsonine inpatients with advanced malignancies, Clinical Cancer Research., 3:1077-1086.
  4. Hao, B.C., Yang, X.P., Wang, X.H., Chen, H., Guo, W.Z., Guo, Z.T., Liu, Y., Shang, R.F., Hu, Y.H. and Liang, J.P. (2013). Study on the Extraction Technology of Swainsonine from Astragalus strictus. Hubei Agricultural Science., 528    :1886-1888. 
  5. Liu, J.X., Chen, F.X., Lu, D.Y., Chen, S.X. and Qian, F.R. (1996). Preliminary study on the mechanism of anti-coxsackievirus B of sophora flavescens alkaloids. Acta University Medicine Second Shanghai., 10:183-185. 
  6. Li, Z. (2009). Research on the traditional Chinese medicine depestia with the effect of anti-influenza virus. Wuhan: Hubei chinese medicine College Master Thesis., 32-33. 
  7. Philip,E.S., Walter, S., Paul, E., Jennifer, K., Eric, W. and Ronald, M.B. (2005). Phase II study of the efficacy and safety of oral GD0039 in patients with locally advanced or metastatic renal cell carcinoma. Investigational New Drugs., 23:577-581.
  8. Singh, D. and Kaur, G. (2011). Optimization of different process variables for the production of an indolizidine alkaloid,swainsonine from Metarhizium anisopliae. Journal of Basic Microbiology., 10:1072-1078.
  9. Song,Y.Y., Zhao, B.Y., Lu, H., Xu, X.J., Tuo, X.L., Wang, J.Y. and Li, R. (2012). The Alkaloids Analysis of Oxytropis deflexa by the Thinlayer Chromatography and Separation of Swainsonine. Acta Agricultural Boreali-occidentalis Sinica., 21:25-29. 
  10. Wang L R, Dong, Y. J. and Liang, M.L. (2005). Etiology, diagnosis and treatment of bovine viral diarrhea virus. Journal of Anhui Agricultural Sciences., 33:2348-2349. 
  11. Zhang, S.Y., Ao, Y.G.R.L. and Wang, D.D. (2012). Investigation and prevention locoweed poisoning in livestock Jilantai region. China Animal Husbandry and Veterinary Digest., 28: 81.
  12. Zhao,Y.L., Zuo, Y.Z., Fan, J.H., Zhang, N., Qin, J.M., Liu, Z. M. and Yang, H. C. (2006). Biological characteristics of bovine viral diarrhoea-mucosal disease virus HB strain. Chinese Agricultural Science Bulletin., 22:1-4. 
  13. Zhao, Q. M.,Yu, Y.T., He, S. H. and Wang, J.H. (2012). Research advances in sources and biosynthesis of swainsonine. Journal ofAgricultural Science. 33:82-88. 
  14. Zhou,Q.W., Zhao, B. Y., Lu, H., Wang, S.S., Zhang, L.,Wen, L.W. and Yang, X.W. (2013). The Research and Control Situation of Ecology and Animal Poisoning of Locoweed in Western Natural Grassland of China. Scientia Agricultura Sinica., 46:1280-1296. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)