Chief EditorK.M.L. Pathak
Print ISSN 0367-6722
Online ISSN 0976-0555
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Peptides isolation from crude somatic antigens of Haemonchus contortus through SDS- PAGE
First Online 29-07-2017|
Haemonchus (H.) contortus is a blood feeding gastrointestinal nematode, and is considered one of the major threats for goat health and production globally. Crude somatic antigens (CSA) have been reported as sources of immunogens and provide better level of immunity against gastrointestinal nematodes. The present study aimed to quantify CSA of H. contortus worms (n=25) of both sexes followed by isolation of peptides through Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and their characterization. The triturate contained proteins having concentration of 1.3mg/ml as measured through Bradford assay. Seven different bands were appeared on gel ranging from 35KDa to 170KDa. The future prospects may include identification of the immunogenic peptides which can be used as vaccine candidates against H. contortus.
- Abd El-Rahman, E.H.(1990). Serological associated proteins from the adult parasite characterization and partial purification of sheep Gastro-intestinal parasites Trichostrongylus colubriformis and Haemonchus contortus antigens. M. Sc. Thesis, Faculty of Science, Cairo University, Egypt.
- Alunda, J.M., Angulo-Cubillan, F. and Cuquerella, M. (2003). Immunization against ovine haemonchosis with three low molecular weight somatic antigens of adult Haemonchus contortus. J.Vet. Med., 50: 70-74.
- Bradford, M. M. (1976). A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principles of protein dye binding. Ann. Biochem., 72: 248–252.
- Derbala, A.E.A. and Abd El-Rahman, E.H. (2001). Potency of three Haemonchus contortus antigens in the diagnosis of ovine haemonchosis. J. Egyptian Society Parasitol., 31: 701-710.
- Dicker, A. J., N. F. Inglis., E. D.T.Manson., S. Subhadra and M. Illangopathy M. (2014). Proteomic Analysis of Mecistocirrus digitatus and Haemonchus contortus intestinal protein extracts and subsequent efficacy testing in a vaccine trial. Plos. Negl. Trop. Dis., 8(6): 2909- 2914.
- Gamble, H. R., and Mansfield L.S. (1996). Characterization of excretory-secretory products from larval stages of Haemonchus contortus cultured in vitro. Vet. Parasitol., 62(3): 291-305.
- Gomez-Munoz M.T, M. Cuquerella and Alunda J.M. (1996). Identification and partial purification of a 26 kilodalton antigen of adult Haemonchus contortus. Int. J. Parasitol., 26:311–318.
- Irfan-ur-Rauf, Tak., M. Z. Chishti and Fayaz A. (2013). Applications of studying Electrophoretic pattern of Haemonchus contortus as tool for vaccine designing - A Review. New York Sci. J., 6(9).60-65.
- Kaur, K., J. Kapur, A. Parmar and Sood M.L. (2002). Identification of immunodominant antigens of adult Haemonchus contortus (Nematoda: Trichostrongylidae). J. Vet. Med. Series B., 49: 260- 262.
- Knox, D. P., Smith S. K., and Smith W. D. (1999). Immunization with an affinity purified protein extract from the adult parasite protects lambs against infection with Haemonchus contortus. Parasite Immunol., 21:201-210.
- Knox, D. P., Smith W. D. (2001). Vaccination against gastrointestinal nematode parasites of ruminants using gut-expressed antigens. Vet. Parasitol., 100 : 21-32.
- Laemmli, U.K.(1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685.
- Meshgi, B and Hosseini, S.H. (2007).Evaluation of different antigens in Western blotting technique for the diagnosis of Sheep Haemonchosis. Iranian J. Parasitol., 2(4): 12-16
- Mir, R. A, M.Z. Chishti., M.A. Zargar., T. Hidayathullah and Ganie S.A. (2008). Excretory-secretory antigens are better than crude antigens for serodiagnosis of Haemonchus contortus. Asian J. Sc.i Res. 1(2):171–175.
- Molento, M. (2009). Parasite control in the age of drug resistance and changing agricultural practices. Vet. Parasitol.163: 229–234.
- Nayebzadeh, H., Seyfiabad Shapouri, M.R., Hoghooghi-Rad, N., and Masouri, B. 2008. Immunization of Arabian sheep with whole gut homogenate of Haemonchus contortus. Iranian J. Vet. Res. Shiraz University., 9(4): 360-364.
- Prasad, A., A. Nasir and Singh N. (2008). Immunodominant polypeptides in somatic antigen of adult Haemonchus contortus by western blotting and immunoprecipitation. Indian J. Animal Sci., 77(7):533–537.
- Roger, P.A. (2008). The impact of disease and disease prevention on welfare in sheep. In: Dwyer, C.M. (Ed.). Animal welfare volume 6: The Welfare of Sheep, Springer. Pp. 159.
- Schallig, H.D.F.H., Leeuwen V.M.A.W., and Hendrix, W.M.L. 1994. Immune response of Texel sheep to excretory/Secretory product of adult Haemonchus contortus. Parasitol.,108: 351-355.
- Smith, W.D., Smith, S.K. (1993). Evaluation of aspects of the protection afforded to sheep immunised with a gut membrane protein of Haemonchus contortus. Res. Vet. Sci., 55: 1–9.
- Soulsby, E.J.L., (1982). Helminths, Arthropods and Protozoa of Domesticated Animals, 7th edition. Bailliere Tindall and Cassel Ltd., London.
- Torres-Acostaa, J.F.J., Mendoza-de-Givesb, A.J.P., Aguilar-Caballeroa, J.A., and Cuéllar-Ordazc. (2012). Anthelmintic resistance in sheep farms: Update of the situation in the American continent. Vet. Parasitol., 189: 89– 96.
- Wolstenholme, A.J., Fairweather, I., Prichard, R., Samson-Himmelstjerna, V.G., and Sangster, N.C. (2004). Drug resistance in veterinary helminths. TrendsParasitol., 20: 469–476.
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