Polymorphisms of the Myostatin gene (MSTN) and its association with growth traits in Bali cattle 

DOI: 10.18805/ijar.v0iOF.7609    | Article Id: B-541 | Page : 817-820
Citation :- Polymorphisms of the Myostatin gene (MSTN) and its associationwith growth traits in Bali cattle .Indian Journal Of Animal Research.2017.(51):817-820

Hary Nugroho, Woro Busono and Sucik Maylinda

jurnal4capa@gmail.com
Address :

Departement of Animal Production, Faculty of Animal Husbandry, University of Brawijaya, Malang, Indonesia.

Submitted Date : 22-05-2016
Accepted Date : 30-09-2016

Abstract

This research aims to develop a method for selection based on myostatin gene markers. The experiment was conducted in Bali cattle. Blood samples were collected from 100 cattle  irrespective of age. PCR-RFLP was applied with primers for amplifying the 1346bp of the Myostatin gene were (F) 5’-CCCTACAGAGGCCACTTCAA-3’ and (R) 5’-CTCGCTGTTCTCATTCAGATC-3’, sand HaeIII restriction enzymes. PCR-RFLP results showed that not all genotypes present in the population, despite populations that were observed quite polymorphic (PIC = 49.5%) with the conditions of gene frequencies. The results showed that almost all variables influenced by myostatin genotype where the genotype BB is better, except the chest girth showed a better result in genotype AB. It suggested that MSTN is strong candidate gene that influenced growth traits. Moreover, it is also expected that this MSTN could be used in marker-assisted selection.

Keywords

Bali cattle Growth traits Myostatin gene Polymorphism PCR-RFLP.

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