Comparative anlysis of the differential expressed proteins in serum between healthy and Brucellaovis infected sheep

DOI: 10.18805/ijar.10978    | Article Id: B-424 | Page : 910-915
Citation :- Comparative anlysis of the differential expressed proteins in serumbetween healthy and Brucellaovis infected sheep .Indian Journal Of Animal Research.2017.(51):910-915

Guoping Wang  and Run Wu

gpwang_2015@163.com
Address :

College of Veterinary Medicine, Gansu Agricultural University, Lanzhou,Gansu Province, 730070, P.R. China.

Submitted Date : 23-12-2015
Accepted Date : 28-04-2016

Abstract

Disease-related serum proteins in sheep infected with Brucellaovis were assessed by conventional two-dimensional gel electrophoresis (2-DE). High-abundance proteins in serum were depleted by Albumin & IgG Depletion SpintrapTM, with the remaining proteins retrieved using the 2-D clean-up kit. Differentially expressed proteins were detected with the PDQUest8.0 software and identified by a high performance liquid chromatography system equipped with an ion trap mass spectrometer. Both SDS-PAGE and 2-DE gel electrophoresis confirmed that high-abundance protein components were removed thoroughly by the 2-D clean-up kit and Albumin & IgG Depletion SpintrapTM. A total of 9 differentially expressed protein spots were successfully identified as haptoglobin, serum amyloid A, Ig heavy chain, Igl-1 chain, and apolipoprotein C-III, known to be involved in stress response, energy metabolism, and material transport. These findings provide a valuable clue for investigating host immune response to pathogens at the protein level; further understanding of these novel proteins identified would be helpful for finding out potential candidate targets for diagnosis and developing new vaccines for brucellosis.

Keywords

Brucellaovis Proteome Sheep Two-dimensional gel electrophoresis.

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