Effect of different doses of  FSH on superovulation, production and quality of embryo in North Omani Cattle breed

DOI: 10.18805/ijar.v0iOF.7003    | Article Id: B-398 | Page : 8-14
Citation :- Effect of different doses of FSH on superovulation, productionand quality of embryo in North Omani Cattle breed .Indian Journal Of Animal Research.2017.(51):8-14

A.M. Hussein, Y.O. Al-Shakaili*, A.N. Al-Ismaily and H.H. Al-Alawi1

a.y9114@gmail.com
Address :

Reproduction Research Department, Rumais Livestock Research Centre, Directorate General of Agriculture & Livestock Research, Ministry of Agriculture, Barka, Sultanate of Oman.

Submitted Date : 17-11-2015
Accepted Date : 24-11-2016

Abstract

The experiment was conducted to investigate the Ovarian response and embryo quality using different doses of follicle stimulating hormone (FSH-P) in North Omani Cattle breed (NOC) in order to determine the suitable dose. Eighteen donor NOC were divided into three groups (G1, G2, and G3) comprising of six cows in each group and treated with 20 (G1), 25 (G2) and 30 mg FSH (G3). Ovarian response was recorded as development of corpus luteum (CL), unovulated follicles (UOF), unfertilized ova (UFO), recovered embryo (RE) and transferable embryos (TE). Embryos were flushed seven days after the first AI and classified according to the developmental stage and quality. Results showed that, cows superovulated with FSH (G3) increased significantly (P<0.05) number of CLs and number of  RE  (6.83 and 5.33 /flushed cow) compared with the G1 of  FSH  (3.17 and 2.00/flushed cow) and G2 (4.67 and 3.00/flushed cow). While using FSH in (G2) increased significantly (P<0.05) the number of TE (excellent and good) quality, decreased average number of UOF and UFO (2.84, 3.33 and 0.67/flush) compared with FSH G3 (1.83, 5.67 and 1.50/flush). While no significant difference was observed in TE number between G1 and G2. G3 groups producing embryos at early stages of development (4 and 8 cells) and this was undesirable for embryo transfer program as this treatment (30 mg) produced more number of fair, poor and degenerated embryos compared with 20 and 25 mg FSH. Consequently, 25 mg FSH dose increased number of TE, decreased number of UOF and UFO, and that was the suitable dose for superovulation of NOC.  

Keywords

Embryo recovery Evaluation FSH North Omani cattle Superovulation.

References

  1. Adams, G. B. (1999). Comparative patterns of follicle development and selection in ruminants. J. Reprod. Fertil. Suppl., 54: 17-32.
  2. Agarwal, U., Shankar, M.C., Yadav, P.C., Sanwal, S. and Varshney, V.P. (1993). Superovulation, embryo recovery and endocrine response in crossbred cattle treated with PMSG and FSH-P. Indian J. Dairy Sci., 46: 450.
  3. Ali, M. S., Khandoker, M. A. M. Y., Afroz, M. A. and Bhuiyan, A. K. F. H. (2012). Ovarian Response to different dose levels of follicle stimulating hormone (FSH) in different genotypes of Bangladeshi cattle. Asian. J. Anim. Sci. 25: 1 : 52 – 58.
  4. Al-Sinani, Karima, R. (2015). Genetic characterization of Omani cattle using Microsatallite DNA Markers. MSc thesis. Department of Animal and Veterinary Sciences, College of Agriculture and Marine Sciences, Sultan Qaboos Univeresity.
  5. Arora, V. K, Devanathan, T. G., Pattabiraman, S. R. and Edwin, M. J. (1996). Progesterone profile at pre, during and post superovulatory treatment in crossbred cows. Indian J. Anim. Reprod., 17: 88-91.
  6. Barati, F., Niasari_Naslaji, A., Bolourchi, M., Sarhaddi, F., Razavi, K., Naghzali, E. and Thatcher, W.W. (2006). Superovulatory response of Sistani cattle to three different doses of FSH during winter and summer. Theriogenology. 66: 1149-1155.
  7. Barros, C.M., Porto, L.P.C. and Noguera, M.F.G. (2003). Dose-response trial in Bos Taurus vs. Bos indicus cows superovulated with FSH, associated with controlled LH surge and fixed time artificial insemination. Theriogenol., 59: 524-524. 
  8. Baruselli, P.S., Sa Filho, M.F., Martins, C.M., Nasser, L.F., Nogueria, M.F.G., Barros, C.M. and Bo, G.A. (2006). Superovulation and embryo transfer in Bos indicus cattle. Theriogenology. 65: 77-88.
  9. Basile, J. R., Chebel, R. J. and Basile, L. F. (1994). Superovulation of Holstein cows with FSH or PMSG Revista-Barasileira-    de-Reprod. Anim. 18 :131 – 136.
  10. Betteridge, K. J. (1981). An historical look at embryo transfer. J. Reprod. Fert. 62:1-13.
  11. Betteridge, K. J. (2000). Reflections on the golden anniversary of the first embryo transfer to produce a calf. Theriogenology 53:3-10.
  12. Bo, G. A., Hockley, D. K., Nasser, L. F. and Mapletoft, R. J. (1994). Superovulatory response to a single subcutaneous injection of Folltropin-V in beef cattle. Theriogenol. 42:963-975.
  13. Chagas-E-Silva-JN; Cidadao, M. R., Chamusco, S. and Silva-Jnce. (1993). Superovulation in Friesian cows. Public-do so-    Simposio-Inter-de Reprod. Anim., Lous- Portugal,-30-de-Setembro-a-2-de-Outubro., 11: 132.
  14. Driancourt, M. A. (2001). Regulation of ovarian follicular dynamics in farm animals. Implication for manipulation of reproduction, Theriogenology, 55 : 1211 – 1239. 
  15. Duncan, D. B. (1955). Multiple range and Multiple F test. Biometrics, 11: 1.
  16. Gaur, M. and Purohit, G. N. (2007). Follicular dynamics in Rathi (Bos indicus) cattle. Veterinarski Arhiv, 77 : 177-186.
  17. Gonzalez, A., Lussier, J.G., Camtthers, T.D., Murphy, B.D. and Mapletoft, R.J. (1990). Superovulation of beef heifers with Folltropin: a new preparation containing reduced LH activity. Theriogenology. 33: 519-529.
  18. Goulding, D., Williams, D. H., Duffy, P., Boland, M. P. and Roche, J. F. (1990). Superovulation in heifers given FSH initiated either at day 2 or day 10 of the estrus cycle. Theriogenology. 34:767-778.
  19. Hockley, D.K., Bo, G.A., Palasz, A.T., Del Campo, M.R. and Mapletoft, R.J. (1992). Superovulation with a single subcutaneous injection of Folltropin in cow : Effect of dose and site of injection. Theriogenology. 37: 224.
  20. Jousan, F. D, U.tt, M. D., Whitman, S. S., Hinshaw, R.H. and Beal, W. E. (2004). Effects of varying the holding temperature and interval from collection to freezing on post-thaw development of bovine embryos in vitro. Theriogenology, 61: 1193-1201. 
  21. Kanitz, W., Becker, F. and Schneider, F. (2002). Superovulation in cattle: practical aspects of gonadotrophin treatment and insemination. Reproduction Nutrition Development, 42:587-599.
  22. Leingcharoen, N., Thungsanthia, A., Apimeteetumrong, M., Thijae, K., and Glampoon, B. (2010). Superovulatory response of Thai Black cattle to three different doses of FSH. Proceedings of the 7th Kasetsart University, Kamphaeng Saen Campus Annual Conference, Bangkok, Thailand, December 3-7, 2010, pp. 278-285. 
  23. Leingcharoen, N., Thuangsanthia, A., Yiengvisavakul, V. And Apimeteetumrong, M. (2006). Effect of FSH doses on superovulation response and embryo yield in Kao Lum Poon cattle. Proceedings of Enhancement of Reproductive Efficiency and Production of Livestock in Thailand, Chiang Mai, Thailand, 14, pp. 104-106.
  24. Lewis, (1992). Programming donors and Recipients. In: Embryo Transfer and Pregnancy Diagnosis, University of Sydney, Post Graduate Committee in Veterinary Science, University of Sydney, Sydney, Australia, ISBN: 9781875582136, pp: 69-88.
  25. Lopes da Costa L, Chagas e Silva J, Robalo Silva J. (2001). Superovulatory response, embryo quality and fertility after treatment with different gonadotrophins in native cattle. Theriogenology. 2001 Jul 1; 56: 65-77.
  26. Mahgoub O, Babiker HA, Kadim IT, Al-Kindy M, Hassan S, Al-Marzooqi W, Eltahir YE, Al-Abri MA, Al-Khayat A, Al-    Sinani KR, Hilal AlKhanjari H, Costa V, Chen S, Beja-Pereira A (2013). Disclosing the origin and diversity of Omani cattle. Animal Genetics, 44: 336-339.
  27. Marian Ochea, Manuela Pascal, Alexandru Sonea, Alin Ion, Birþoiu (2015).The Effect of Epidural Administration of FSH In Bovine Superovulation Protocol. Animal Science. LVIII, p217-220.
  28. Merton S. (2009). Overall bovine embryo transfer activity in Europe in 2008. Proceedings of the 25th AETE meeting Poznan 11-12th September 2009, p 85.
  29. Mishra, U.K., Mishra, O.P. and Khahj, J.R. (1996). Estrus synchronization, superovulation and non-surgicel embryo transfer in Sahiwal cows (Bos indicus). Indian J. Anim. Sci. 66: 1271-1273.
  30. Neto, A. S., B. V. Sanches, M. Binelli, M. M. Seneda, S. H. Perri and J. F. Garcia. (2005). Improvement in embryo recovery using double uterine flushing. Theriogenology 63:1249-1255.
  31. Nilchuen, P., Rattanatabtimtong, S. and Chomchai, S. (2011) Superovulation with different doses of follicle stimulating hormone in Kamphaeng Saen beef cattle. Songklanakarin J. Sci. Technol. 33: 679-683.
  32. Randel R.D. (1984). Seasonal effects on female reproductive functions in the bovine (Indian breeds). Theriogenology 21: 70-85.
  33. Reddy, S.M. (1994). In vitro fertilization and embryo transfer in cattle. Ph. D. Thesis. Bidhan Chandra Krishi Viswavidyalaya, Mohanpur, West Bengal, Indian.
  34. SAS, (2002). User,s Guide. Release 9.00. Cary, NC: USA Institute.
  35. Shea, B. F., Janzen, R. E. and McDermand, D. P. (1984). Seasonal variation and related embryo transfer procedures in Alberta over a nine year period. Theriogenol. 21:186-195.
  36. Snedecor, G.W and Cochran, W.G. (1982). Statistical methods. 7th Ed. Iowa Univ. Press, Ames. Iowa, USA.
  37. Staigmiller, R. B., Bellows, R. A., Anderson, G. B., Seidel, G. E., Foote, W. D., Menino, A. R. and Wright, R. W. (1992). Superovulation of cattle with equine pituitary extract and porcine FSH. Theriogenol. 37:1091-1099.
  38. Sumretprasong, J., Leangcharuen, N., Thuangsanthia, A. And Thijae, K. (2008). Dose response to superovulation in Thai dairy cattle. Proceedings of The 15th Congress of FAVA-OIE Joint Symposium on Emerging Diseases, Bangkok, Thailand, October 27-30, pp. 247-248.
  39. Thibier M., (2007) New records in the number of both in vivo derived and in vitro produced bovine embryos around the world in 2006. IETS Newsletter. 4: 15-20.
  40. Umut Tasdemir, Muharrem Satilmis, Tahir Karasahin, Sedat Hamdi Kizil, Mustafa Kaymaz, Kei Imai (2012). The Effect of Single Epidural Plus Intramusculer Injection Of FSH On Superovulatory Response In Anatolian Black Cow. Ankara Üniv Vet Fak Derg, 59: 211-216, 2012.

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