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Research Article

volume 54 issue 12 (december 2020) : 1595-1598,   Doi: 10.18805/ijar.B-3914

Histological, Micrometrical and Histochemical Studies on the Testes of Large White Yorkshire Pig (Sus scrofa domesticus)

T
Thokchom Shitarjit Singh
P
P.C. Kalita
O
O.P. Choudhary
A
A. Kalita
P
P.J. Doley
1Department of Veterinary Anatomy and Histology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl-796 015, Mizoram, India.
Cite article:- Singh Shitarjit Thokchom, Kalita P.C., Choudhary O.P., Kalita A., Doley P.J. (2020). Histological, Micrometrical and Histochemical Studies on the Testes of Large White Yorkshire Pig (Sus scrofa domesticus). Indian Journal of Animal Research. 54(12): 1595-1598. doi: 10.18805/ijar.B-3914.

ABSTRACT

The study has been conducted on the testes of 10 adult Large White Yorkshire pig (1.5-2) years of age. The testis was surrounded by a capsule made up of dense irregular connective tissue comprising three layers viz., outer visceral layer of tunica vaginalis, middle tunica albugenia and inner tunica vasculosa. The connective tissue trabeculae were extended from the capsule and divided the parenchyma of the testis into number of lobules and consisted of collagen, elastic and reticular fibers along with rete testis and blood vessels. The Periodic Acid–Schiff shows strong affinity in basement membrane of seminiferous tubules and tunica albuginea with a moderate activity in Leydig cells. The Alcian blue shows strong affinity in basement membrane of seminiferous tubule and weak activity was observed in tunica albuginea and interstitial tissue. The different histomorphometrical parameters with regard to thickness of testicular capsule, diameter and height of seminiferous tubules were found to be higher in the left testicle. 

REFERENCES

  1. Ahmed, A.E. (2005). Morphological, Glycohistochemical and Immunohistochemical Studies on the Embryonic and Adult Bovine Testis a thesis submitted for the Doctor Degree in Veterinary Medicine, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Munich, Egypt.
  2. Bancroft, J.D. and Gamble, M (1997). Theory and Practice of Histological Techniques. 6th ed. Churchill Livingstone, UK.
  3. Bansal, N., Uppal, V., Ashwinikumar and Pathak, D. (2009). Histomorphochemical studies on testis of guinea pig. Indian J. Anim. Sci. 79(8): 809-812.
  4. Bashir, S., Sarma, K., Suri, S., Devi, J. and Kamil, S. (2012). Histochemical studies on the testis of adult Bakarwali Goat (Capra hircus). Indian. J. Vet. Anat. 24(1): 50-51.
  5. Bhosale, P.H. (2016). Gross anatomical and histomorphological studies of testis in goat (Capra hircus). M.V.Sc. Thesis submitted to the Bombay Veterinary College, Mumbai. pp: 27-29.
  6. Bunter, K. and Bennett, C. (2004). Genotype comparisons for meat and eating quality traits. AGBU Pig Genetics Workshop Notes. pp: 59-69.
  7. Comizzoli, P., Mermillod, P., Mauget, R. (2000). Reproductive biotechnologies for endangered mammalian species. Reprod. Nutr. Dev. 40: 493-504.
  8. Costa, G.M.J., Leal, M.C., Ferreira, C.S., Guimarães, D.A. and Franca, L.R. (2010). Duration of spermatogenesis and spermatogenic efficiency in 2 Large Neotropical Rodent Species: The Agouti (Dasyproctaleporina) and Paca (Agouti paca) Duration of spermatogenesis and spermatogenic efficiency in 2 large neotropical rodent species: the agouti (Dasyproctaleporina) and paca (Agouti paca). J Androl. doi: org/10.2164/jandrol.109.009787.
  9. Egger, G.F. and Witter, K. (2009). Peritubular contractile cells in testis and epididymis of the dog, Canis lupus familiaris Acta Vet. Brno.78: 3–11.
  10. Eurell, J.A. and Frappier, B.L. (2006). In: Dellmann’s Textbook of Veterinary Histology. 6th Edn. Blackwell Publishing, Iowa, Ames, USA. pp: 233-245.
  11. Franca, L.R. and Godinho, C.L. (2003). Testis morphometry, seminiferous epithelium cycle length and daily sperm production in domestic cats (Feliscatus). Biol. Reprod. 68(5): 1554-1561.
  12. Gleide F.A., Carolina, F.A.O., Jaqueline, M.S., Israel, J.S., Ina, D., Rex, A.H. and Luiz, R.F. (2010). Postnatal somatic cell proliferation and seminiferous tubule maturation in pigs: A non-random event. Theriogenol. 74: 11-23.
  13. Gofur, M.R., Khan M.Z.I., Karim M.R. and Islam, M.N. (2008). Histomorphology and histochemistry of testis of indigenous bull (BosIndicus) of Bangladesh. Bangl. J. Vet. Med. 6(1): 67-74.
  14. Gomori, G. (1937). Silver impregnation of reticulum in paraffin sections. Am. J. Physiol. 13: 993.
  15. Kishore, P.V.S., Geetha Ramesh and Sabiha Hayath Basha (2011). Sertoli cells in the testis of ram- A postnatal histological study. Indian Journal of Veterinary Anatomy. 23(1): 56-59.
  16. Luna, L.G. (1968). Manual of Histological Staining Methods of Armed Force Instituate of Pathology. 3rd Edn. McGraw-Hill Book Company, New York. pp: 87- 88, 94-95.
  17. Masson, P. (1929). Some histological methods trichromestainings and their preliminary technique. Bull. Inter. Assoc. Med. 12: 75.
  18. Mayer, P. (1891). Ueber das Forbenmithaematoxylin. Mitt. Zool. Stat. Neapel. 10:170-186.
  19. Moonjit, P. and Adcharatt, S. (2007). Histological structure of testes and ductus epididymis of Rusa deer (Cervustimorensis). Kasetsart Journal (Nat. Sci.) 41: 86-90.
  20. Ohanian, C., Rodriguez, H, Piriz, H., Martino, I., Rieppi, G., Garofalo, E.G. and Roca R.A. (1979). Studies on the contractile activity and ultrastruture of the boar testicular capsule. Journal of Reproduction and Fertility. 57: 79-85.
  21. Parul, S. (2015). Prenatal study on the development of testis and epididymis of gaddi sheep. Phd. Thesis. Department of Veterinary Anatomy, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya Palampur-176062 (H.P.), pp. 75-80.
  22. Shitarjit, S.T., Kalita, P.C., Choudhary, O.P., Kalita, A. and Doley, P.J. (2018). Histomorphological studies on the testis of local pig (Zovawk) of Mizoram. Indian J. Anim. Res. 53 (11): 1-4.
  23. Shukla, P., Bhardwaj, R. L. and Rajput, R. (2013). Histomorphology and micrometry of testis of Chamurthi Horse. Indian J. Vet. Anat. 25(1): 36-38.
  24. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods, 8th Edn. Lowa State University Press, pp. 313.
  25. Sulabh, S., P.Shivhare, A. Kumari, M. Kumar and R. Nimmanapalli, (2017). Status of pig rearing in India. Int. J. Vet. Sci. Ani. Husbandry. 2(3): 30–32
  26. Taylor, G., Roese, G. and Hermesch, S. (2005). Breeds of pigs-    Large White. Primefact. 62: 1-3.
  27. Verhoeff, F.H. (1908). Some new staining methods of wide applicability including a rapid differential stain for elastic tissue. J. Am. Med. Assoc. 50: 876-877.
  28. Yaseen, S.M. (2009). Gross and histological studies on the testes of Marwari goat (Capra hircus). M.V.Sc. Thesis submitted to the Swami Keshwanand Rajasthan Agricultural University, Bikaner. pp: 1-46.
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    Research Article

    volume 54 issue 12 (december 2020) : 1595-1598,   Doi: 10.18805/ijar.B-3914

    Histological, Micrometrical and Histochemical Studies on the Testes of Large White Yorkshire Pig (Sus scrofa domesticus)

    T
    Thokchom Shitarjit Singh
    P
    P.C. Kalita
    O
    O.P. Choudhary
    A
    A. Kalita
    P
    P.J. Doley
    1Department of Veterinary Anatomy and Histology, College of Veterinary Sciences and Animal Husbandry, Central Agricultural University, Selesih, Aizawl-796 015, Mizoram, India.
    Cite article:- Singh Shitarjit Thokchom, Kalita P.C., Choudhary O.P., Kalita A., Doley P.J. (2020). Histological, Micrometrical and Histochemical Studies on the Testes of Large White Yorkshire Pig (Sus scrofa domesticus). Indian Journal of Animal Research. 54(12): 1595-1598. doi: 10.18805/ijar.B-3914.

    ABSTRACT

    The study has been conducted on the testes of 10 adult Large White Yorkshire pig (1.5-2) years of age. The testis was surrounded by a capsule made up of dense irregular connective tissue comprising three layers viz., outer visceral layer of tunica vaginalis, middle tunica albugenia and inner tunica vasculosa. The connective tissue trabeculae were extended from the capsule and divided the parenchyma of the testis into number of lobules and consisted of collagen, elastic and reticular fibers along with rete testis and blood vessels. The Periodic Acid–Schiff shows strong affinity in basement membrane of seminiferous tubules and tunica albuginea with a moderate activity in Leydig cells. The Alcian blue shows strong affinity in basement membrane of seminiferous tubule and weak activity was observed in tunica albuginea and interstitial tissue. The different histomorphometrical parameters with regard to thickness of testicular capsule, diameter and height of seminiferous tubules were found to be higher in the left testicle. 

    REFERENCES

    1. Ahmed, A.E. (2005). Morphological, Glycohistochemical and Immunohistochemical Studies on the Embryonic and Adult Bovine Testis a thesis submitted for the Doctor Degree in Veterinary Medicine, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Munich, Egypt.
    2. Bancroft, J.D. and Gamble, M (1997). Theory and Practice of Histological Techniques. 6th ed. Churchill Livingstone, UK.
    3. Bansal, N., Uppal, V., Ashwinikumar and Pathak, D. (2009). Histomorphochemical studies on testis of guinea pig. Indian J. Anim. Sci. 79(8): 809-812.
    4. Bashir, S., Sarma, K., Suri, S., Devi, J. and Kamil, S. (2012). Histochemical studies on the testis of adult Bakarwali Goat (Capra hircus). Indian. J. Vet. Anat. 24(1): 50-51.
    5. Bhosale, P.H. (2016). Gross anatomical and histomorphological studies of testis in goat (Capra hircus). M.V.Sc. Thesis submitted to the Bombay Veterinary College, Mumbai. pp: 27-29.
    6. Bunter, K. and Bennett, C. (2004). Genotype comparisons for meat and eating quality traits. AGBU Pig Genetics Workshop Notes. pp: 59-69.
    7. Comizzoli, P., Mermillod, P., Mauget, R. (2000). Reproductive biotechnologies for endangered mammalian species. Reprod. Nutr. Dev. 40: 493-504.
    8. Costa, G.M.J., Leal, M.C., Ferreira, C.S., Guimarães, D.A. and Franca, L.R. (2010). Duration of spermatogenesis and spermatogenic efficiency in 2 Large Neotropical Rodent Species: The Agouti (Dasyproctaleporina) and Paca (Agouti paca) Duration of spermatogenesis and spermatogenic efficiency in 2 large neotropical rodent species: the agouti (Dasyproctaleporina) and paca (Agouti paca). J Androl. doi: org/10.2164/jandrol.109.009787.
    9. Egger, G.F. and Witter, K. (2009). Peritubular contractile cells in testis and epididymis of the dog, Canis lupus familiaris Acta Vet. Brno.78: 3–11.
    10. Eurell, J.A. and Frappier, B.L. (2006). In: Dellmann’s Textbook of Veterinary Histology. 6th Edn. Blackwell Publishing, Iowa, Ames, USA. pp: 233-245.
    11. Franca, L.R. and Godinho, C.L. (2003). Testis morphometry, seminiferous epithelium cycle length and daily sperm production in domestic cats (Feliscatus). Biol. Reprod. 68(5): 1554-1561.
    12. Gleide F.A., Carolina, F.A.O., Jaqueline, M.S., Israel, J.S., Ina, D., Rex, A.H. and Luiz, R.F. (2010). Postnatal somatic cell proliferation and seminiferous tubule maturation in pigs: A non-random event. Theriogenol. 74: 11-23.
    13. Gofur, M.R., Khan M.Z.I., Karim M.R. and Islam, M.N. (2008). Histomorphology and histochemistry of testis of indigenous bull (BosIndicus) of Bangladesh. Bangl. J. Vet. Med. 6(1): 67-74.
    14. Gomori, G. (1937). Silver impregnation of reticulum in paraffin sections. Am. J. Physiol. 13: 993.
    15. Kishore, P.V.S., Geetha Ramesh and Sabiha Hayath Basha (2011). Sertoli cells in the testis of ram- A postnatal histological study. Indian Journal of Veterinary Anatomy. 23(1): 56-59.
    16. Luna, L.G. (1968). Manual of Histological Staining Methods of Armed Force Instituate of Pathology. 3rd Edn. McGraw-Hill Book Company, New York. pp: 87- 88, 94-95.
    17. Masson, P. (1929). Some histological methods trichromestainings and their preliminary technique. Bull. Inter. Assoc. Med. 12: 75.
    18. Mayer, P. (1891). Ueber das Forbenmithaematoxylin. Mitt. Zool. Stat. Neapel. 10:170-186.
    19. Moonjit, P. and Adcharatt, S. (2007). Histological structure of testes and ductus epididymis of Rusa deer (Cervustimorensis). Kasetsart Journal (Nat. Sci.) 41: 86-90.
    20. Ohanian, C., Rodriguez, H, Piriz, H., Martino, I., Rieppi, G., Garofalo, E.G. and Roca R.A. (1979). Studies on the contractile activity and ultrastruture of the boar testicular capsule. Journal of Reproduction and Fertility. 57: 79-85.
    21. Parul, S. (2015). Prenatal study on the development of testis and epididymis of gaddi sheep. Phd. Thesis. Department of Veterinary Anatomy, Chaudhary Sarwan Kumar Himachal Pradesh Krishi Vishvavidyalaya Palampur-176062 (H.P.), pp. 75-80.
    22. Shitarjit, S.T., Kalita, P.C., Choudhary, O.P., Kalita, A. and Doley, P.J. (2018). Histomorphological studies on the testis of local pig (Zovawk) of Mizoram. Indian J. Anim. Res. 53 (11): 1-4.
    23. Shukla, P., Bhardwaj, R. L. and Rajput, R. (2013). Histomorphology and micrometry of testis of Chamurthi Horse. Indian J. Vet. Anat. 25(1): 36-38.
    24. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Methods, 8th Edn. Lowa State University Press, pp. 313.
    25. Sulabh, S., P.Shivhare, A. Kumari, M. Kumar and R. Nimmanapalli, (2017). Status of pig rearing in India. Int. J. Vet. Sci. Ani. Husbandry. 2(3): 30–32
    26. Taylor, G., Roese, G. and Hermesch, S. (2005). Breeds of pigs-    Large White. Primefact. 62: 1-3.
    27. Verhoeff, F.H. (1908). Some new staining methods of wide applicability including a rapid differential stain for elastic tissue. J. Am. Med. Assoc. 50: 876-877.
    28. Yaseen, S.M. (2009). Gross and histological studies on the testes of Marwari goat (Capra hircus). M.V.Sc. Thesis submitted to the Swami Keshwanand Rajasthan Agricultural University, Bikaner. pp: 1-46.
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