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Current IssueEditorial BoardOnline First Articles
Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Research Article

Indian Journal of Animal Research, volume 54 issue 11 (november 2020) : 1415-1421

Screening of Antimicrobial Residues and Confirmation of Doxycycline in Samples Collected from Chicken Farms and Processing Units Located Around Mumbai, India

R.N. Waghamare, A.M. Paturkar, V.M. Vaidya, R.J. Zende, Amit Kumar, J.S. Bedi
1Department of Veterinary Public Health, Department of Veterinary Public Health College of Veterinary and Animal Sciences, Parbhani-431 402, Maharashtra, India.
Cite article:- Waghamare R.N., Paturkar A.M., Vaidya V.M., Zende R.J., Kumar Amit, Bedi J.S. (2020). Screening of Antimicrobial Residues and Confirmation of Doxycycline in Samples Collected from Chicken Farms and Processing Units Located Around Mumbai, India. Indian Journal of Animal Research. 54(11): 1415-1421. doi: 10.18805/ijar.B-3899.

ABSTRACT

The presence of antimicrobial residues in meat has several impacts on health aspects to the consumer. Strategic and scientific study on risk assessment of antibiotic residue in poultry production chain with different set of practices has not been conducted in order to protect the health of common consumers. The present study was planned to screen antibiotic residue in chicken meat production chain managed under different set of practices of farming and processing. Chicken farms managed under non-integrated, partially integrated and completely integrated sector and three different individual chicken processing units identified as retail shops, semi-automatic processing unit and automated processing unit along with one product processing unit were randomly selected for the collection of samples. A total of 271 samples (meat, serum, water and feed) comprising of 180 samples from poultry farms, 81 samples from chicken processing units and 10 muscle samples from poultry product processing units were collected and subjected for qualitative antimicrobial screening by Microbial Inhibition Assay Test Kit and confirmation by High Performance Liquid Chromatography /Liquid Chromatography Mass Spectrometry was done. On screening by Microbial Inhibition Assay Test Kit, Muscle (2), Liver (2) and Feed (7) samples (6.11%) were positive from non-integrated (3.88%) and partially integrated farming systems (2.22%). Significant difference (P<0.05) was observed for antibiotic residue in feed samples of non-integrated (55.55%) and partially integrated (22.22%) farms. On screening of 91 samples, collected from chicken processing and product processing units, 08 (8.79%) samples were -positive. The HPLC analysis indicated that 9.5% and 5.26% muscle and liver samples, respectively were positive for doxycycline residue. Feed samples (57.14%) found positive to Chlortetracycline and 4-Epichlorotetracycline residues when analyzed by LCMS, with mean concentration of 2722.12 and 3720.87µg/kg, respectively. The presence of antibiotic residues in muscle and liver samples of unorganized farms and retail processing unit was higher in comparison. Therefore, the poultry farming and processing systems should be kept under continuous monitoring for preventing the occurrence of antibiotic residues in meat and meat products. 

REFERENCES

  1. Aarestrup, F. M. (2003). Effects of termination of AGP use on antimicrobials resistance in food animals. Working papers for the WHO international review panels evaluation,” Document WHO/CDS/CPE/ZFK/2003.1a, World Health Organization, Geneva, Switzerland.
  2. Al-Ghamdi, M., Z. Al-Mustafa, F. El-Morsy, A. Al-Faky, I. Haider and H. Essa, H (2000). Residues of tetracycline compounds in poultry products in the eastern province of Saudi Arabia Public Health. 114 :300–304.
  3. Apata D. F. (2009). Antibiotic Resistance in Poultry. International Journal of Poultry Science. 8 (4): 404-408.
  4. Bogialli, S. and A. D. Corcia (2009). Recent applications of liquid chromatography mass spectrometry to residue analysis of antimicrobials in food of animal origin. Analytical and Bioanalytical Chemistry. 395: 947–966.
  5. Bute V. S. (2008). Detection of antimicrobial residues in chicken meat. M.V.Sc thesis submitted to Maharashtra animal and Fishery Sciences University Nagpur.
  6. Cetinkayaa, F., A. Yibara, G.E. Soyutemiza, B. Okutanb, A. Ozcanc and M.Y. Karacac (2012). Determination of tetracycline residues in chicken meat by liquid chromatography-tandem mass spectrometry. Food Additives and Contaminants: Part B: Surveillance. 5(1): 45-49.
  7. Chinchilla F. B. and C. Rodríguez (2017). Journal of Analytical Methods in Chemistry, Article ID 1315497 in press.
  8. CSE Study (2014). Report on Antibiotics in Chicken Meat, Centre for science and environment 41, Tughlakabad Institutional area, New Delhi. 
  9. Dewdney, J.M., L. Maes, J. P. Raynaud, F. Blanc, J. P. Scheid, T. Jackson, S. Lens, C. Verschueren (1991). Risk assessment of antibiotic residues of â-lactams and macrolides in food products with regard to their immunoallergic potential. Food and Chemical Toxicology. 29: 477–483. 
  10. Ezenduka, E.V., O.S. Ike, and N.J. Anaelom (2014). Rapid detection of antimicrobial residues in poultry: A consequence of non-    prudent use of antimicrobials. Health. 6(2): 149-152.
  11. Food Drug Administration (2016). NARMS Now (ed.) Food and Drug Administration. (Rockville, MD: U.S. Department of Health and Human Services). Available from:https://www.fda. gov/animalVeterinary/SafetyHealth/Antimicrobial Resistance/    NationalAntimicrobialResistanceMonitoringSystem/ucm 416741.htm.
  12. Gaudin V, Juhel-Gaugain M, Mortain J-P, Sanders P (2008). AFNOR validation of Premi®Test, a microbiological-based screening tube-test for the detection of antimicrobial residues in animal muscle tissue Food Additives and Contaminants. 25:1451–1464.
  13. Gebre B.A. (2012). Qualitative screening of antibiotic residues and identification of antibiotic resistant Salmonella from raw and ready to eat meat in Thailand. International Journal of Advanced Life Sciences. 5(1): 51-64.
  14. Gilchrist, M. J., C. Greko, D. B. Wallinga, G. W. Beran, D. G. Riley and P. S. Thorne (2007). The potential role of concentrated animal feeding operations in infectious disease epidemics and antibiotic resistance. Environmental Health Perspectives. 115:313–316. 
  15. GOI (2019). Government of India (GOI) Basic Animal Husbandry Statistics. Ministry of Agriculture. Department of Animal Husbandry, Dairying and Fisheries. New Delhi.
  16. Hussein, M.A. and S. Khalil (2013). Screening of some antibiotics and anabolic steroids residues in broiler fillet marketed in El-Sharkia governorate. Life Science Journal. 10(1): 2111-2118.
  17. Jensen H. H. and D. J. Hayes (2014). Impact of Denmark’s ban on antimicrobials for growth promotion. Current Opinion in Microbiology. 19(1):30–36.
  18. Kiilholma, J. (2007). Food-safety concerns in the poultry sector of developing countries In. Lesna, I., Wolfs, P., Faraji, F., Roy, L.,Komdeur, J. and Sabelis, M.W. (2009). Candidate predators for biological control of the poultry red mite Dermanyssus gallinae. Experimental and Applied Acarology. 48(1–2): 63-80.
  19. Kim, D. P., G. Degand, C. Douny, G. Pierret, P. Delahaut, V. D. To, B. Granier, M. L. Scippo (2013a). Preliminary Evaluation of Antimicrobial Residue Levels in Marketed Pork and Chicken Meat in the Red River Delta Region of Vietnam. Food and Public Health. 3(6): 267-276.
  20. Kim, D. P., C. Saegerman, C. Douny, T. V. Dinh, B. H. Xuan, B. D. Vu, N. P. Hong and M. Scippo (2013b). First Survey on the Use of Antibiotics in Pig and Poultry Production in the Red River Delta Region of Vietnam. Food and Public Health. 3(5): 247-256
  21. Kodimalar K., R. A. Rajini, S. Ezhilvalavan and G. Sarathchandra (2014). As survey of chlortetracycline concentration in feed and its residue in chicken egg in commercial layer farms. Journal of Biosciences. 39:425–431.
  22. Kotaiah T. (2016). Poultry Production in India - The Current Scenario. FnBnews.com.http://www.fnbnews.com/Poultry/poultry-    production-in-india—the-current-scenario-38620. Last accessed on 20.01.2019.
  23. Laxminarayan, R. and R. R. Chaudhury (2016). Antibiotic Resistance in India: Drivers and opportunities for Action. PLoS Medicine. 13(3): e1001974. 
  24. Marcinèák, S., K. Hussein, N. Zdolec, J. Janošová (2006). Premi®test - Fast screening test for detection of sulphadimidine residues in poultry tissues. Meso (VII) rujan - listopad br. 5.
  25. Maria C., V. Trifan, G. Paraschiv and A. Rotaru. (2007). The depletion of doxycycline residues in poultry meat. Bulletin USAMV-    CN. 64 :1-2.
  26. McGettigan, P., P. Roderick, A. Kadam and A. Pollock (2018). Threats to global antimicrobial resistance control: Centrally approved and unapproved antibiotic formulations sold in India. British Journal of Clinical Pharmacology. Published Online: February 5. 2018.
  27. Nikaido, H. (2009). Multidrug Resistance in Bacteria Annual Review of Biochemistry. 2009; 78: 119–146.
  28. Okerman L., S. Croubels, S. De Baere, J. Van Hoof, P. De Backer and H. De Brabander (2001). Inhibition tests for detection and presumptive identification of tetracyclines, beta-lactam antibiotics and quinolones in poultry meat. Food Additives and Contaminants. 18(5): 385-393.
  29. Oyekunle, M. A., S. A. Shodiya and I. K. Jimoh (2003). Reservoir of antimicrobial Resistant Salmonellae among poultry in a Local Government area in Ogun State, Nigeria. Asset Journal International Series A. 3(4): 71 – 80.
  30. Refsdal, A. O. (2000). To treat or not to treat: a proper use of hormones and antibiotics Anim. Reprod. Sci. 60(61): 109-119.
  31. Sakdinun, P., T. Paethaisong and S. Sirilux (2006). Chlotetracycline and Oxytetracycline in Animal Feed from 4 Western Provinces Thai-NIAH eJournal: http://www.dld.go.th/niah. 1 (2):143 -146.
  32. Salama, N.A., S.H. Abou-Raya, A.R. Shalaby, W.H. Emam and F.M. Mehaya (2011). Incidence of tetracycline residues in chicken meat and liver retailed to consumers. Food Additives and Contaminants: Part B. 4(2):88–93.
  33. Sattar, S., M.M. Hassan, S.K.M.A. Islam, M. Alam, M.S.A. Faruk, S. Chowdhury, A.K.M. Saifuddin (2014). Antibiotic residues in broiler and layer meat in Chittagong district of Bangladesh. Veterinary World. 7(9): 738-743.
  34. Senyuva, H., T. Ozden and D. Y. Sarica (2000). High-Performance Liquid Chromatographic Determination of Oxytetracycline Residue in Cured Meat Products. Turkish Journal of Chemistry. 24:395-400.
  35. Singh, R. P., Y. P. Sahni, S. Bharti and N. Chandra (2016). Determination of Residual Concentration of Doxycycline In Chicken Meat. Journal of Cell and Tissue Research. 16(3): 5825-5828. 
  36. Siwczyñska M. P., E. Patyra, M. C. Purcha³a, B.Kozak and K. Kwiatek. (2015). Occurrence of tetracyclines in feedingstuffs-results of a two-year study within the official control of feed. Bulletin of the Veterinary Institute in Pulawy. 59: 527-532.
  37. USDA (2015). Global Agricultural Information Network- Poultry and Poultry Products Annual Report. GAIN Report No IN5124 (2015). 
  38. Waghamare RN, Paturkar AM, Vaidya VM, Zende RJ,Dubal ZN, Dwivedi A, Gaikwad RV (2018). Phenotypic and genotypic drug resistance profile of Salmonella serovars isolated from poultry farm and processing units located in and around Mumbai city, India, Veterinary World. 11(12): 1682-1688.
  39. Wasch, K., L. Okerman, H. De Brabander, J. Van Hoof, S. Croubels and P. De Backer (1998). Detection of residues of tetracycline antibiotics in pork and chicken meat: correlation between results of screening and confirmatory tests. Analyst. 123: 2737–2741.
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