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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 54 issue 11 (november 2020) : 1451-1454,   Doi: 10.18805/ijar.B-3893

Study on Genetic Polymorphism of IGF-1 Gene in Barbari and Jamunapari Goat

M
Mahesh Shivanand Dige
R
Rakesh Kaushik
B
Bhavana Kushwah
M
M.K. Singh
S
Saket Bhusan
P
P.K. Rout
1Animal Genetics and Breeding Division, ICAR-CIRG, Makhdoom, Mathura-281 122, Uttar Pradesh, India. 
Cite article:- Dige Shivanand Mahesh, Kaushik Rakesh, Kushwah Bhavana, Singh M.K., Bhusan Saket, Rout P.K. (2020). Study on Genetic Polymorphism of IGF-1 Gene in Barbari and Jamunapari Goat. Indian Journal of Animal Research. 54(11): 1451-1454. doi: 10.18805/ijar.B-3893.

ABSTRACT

Insulin like growth factor (IGF 1) play an important role in the production and reproduction. One hundred and ninety two adult goats of Barbari and Jamunapari breed were taken for study. Data on growth traits were recorded to identify SNPs in the IGF-1gene by using High Resolution Melting (HRM) technique. Five fragments of IGF-1 were amplified length of amplicon were 105, 124, 150, 122 and 122 bp covering 5’UTR, 3’UTR, Exon 3, Exon 2 and 5’UTR regions, respectively and subsequently sequenced. The HRM analysis revealed no sequence variation among the samples of both breeds. Hence, the all five amplified fragments of IGF-1 gene showed monomorphic pattern and, no significant association could be detected with growth traits in Barbari and Jamunapari goats.

REFERENCES

  1. Brisson, B.K., Barton, E.R. (2012). Insulin-like growth factor-I E-    peptide activity is dependent on the IGF-I receptor. PLoS One. 7(9): e45588.
  2. Curi, R.A., Oliveira, H.N., Silveira, A.C., Lopes, C.R. (2005). Association between IGF-I, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livestock Production Science. 94: 159-167.
  3. Darwish, H.R, El-Shorbagy, H.M, Abdou-Eisha, A.M, El-Din, A.E and Farag, I.M. (2017).New Polymorphism in the 5’ flanking region of IGF1 gene and its association with wool traits in Egyptian Barki Sheep. J Genet Engin Biotech. 15: 437-441.
  4. Daughaday, W.H., Rotwein, P. (1989). Insulin-like growth factors I and II. Peptide, messenger ribonucleic acid and gene structures, serum, and tissue concentrations. Endocrine Reviews. 10:68-91.
  5. Jensen, E. C., Harding, J. H., Bauer, M.K., Gluckman, P.D. (1999). Metabolic effects of IGF-I in the growth retarded fetal sheep. Journal of Endocrinology. 161: 485-494.
  6. Kaushik, R., Dige, M.S., Rout, P.K. (2016). Molecular characterization and expression profiling of ENOX2 gene in response to heat stress in goats. Cell and Development Biology. 5:2. DOI: 10.4172/2168-9296.1000176.
  7. Kaushik, R., Goel, A., Rout, P.K. (2019). Differential and characterization of ATP1A1 exon17 gene by high resolution melting analysis and RT PCR in Indian goats. Molecular Biology Reports. doi.org/10.1007/s11033-019-04984-4.
  8. Kurdistani, Z., Rostamzadeh, J., Rashidi, A., Davis, M.E. (2013). Evaluation of insulin-like growth factor-I gene polymorphism on growth traits and yearling fleece weight in goats. Small Ruminant Research. 111(1): 10–5.
  9. Li, C., Basarab, J., Snelling, W.M., Benkel, B., Murdoch, B., Hansen, C., Moore SS. (2004): Assessment of positional candidate genes myf5 and igf1 for growth on bovine chromosome 5 in commercial lines of Bostaurus. Journal of Animal Science. 82:1-7
  10. Moody, D.E., Pomp, D., Newman, S., Macneil, M.D. (1996). Characterization of DNA polymorphisms in three populations of Hereford cattle and their associations with growth and maternal EPD in line 1 Herefords. Journal of Animal Science. 74: 1784-1793. 
  11. Moradian C., Mohamadi N., Sheshdeh S.A.R., Hajihosseinlo A., Ashrafi F. (2013). Effects of genetic polymorphismat the growth hormone gene on growth traits in Makooei sheep. European Journal of Experimental Biology. 3(3): 101-105.
  12. Naicy, T., Venkatachalapathy, R.T., Aravindakshan, T.V., Kurian, E. (2017).Association of a Cac8I polymorphism in the IGF1 gene with growth traits in Indian goats. Journal of Genetic Engineering and Biotechnology. 15: 7–11. 
  13. Qiong, W., Chao, F., Wu-Jun, L., Yi, F., Shi-Gang, Y. (2011). A novel mutation at exon 4 of IGF-1 gene in three indigenous goat breed sin China. Asian Journal of Animal and Veterinary Advances. 6(6):627-635.
  14. Rasouli, S., Abdolmohammadi, A., Zebarjadi, A. and Mostafei, A., (2017). Evaluation of polymorphism in IGF-I and IGFBP-    3 genes and their relationship with twinning rate and growth traits in Markhoz goats. Ann Anim Sci. 17(1): 89-103.
  15. Rout, P.K., Saxena, V.K., Khan, B.U., Roy, R., Singh, S.K., Singh, L.B. (2000). Characterization of Jamunapari goats in their home tract. Anim Genet Resour Infor. 27:43.
  16. Sankhyan, V., Thakur, Y.P., Dogra, P.K., (2019). Genetic polymorphism in IGF-1 gene in four sheep and goat breeds and its association with biometrical traits in migratory Gaddi goat breed of western Himalayan state of Himachal Pradesh, India. Indian Journal of Animal Research. DOI: 10.18805/    ijar.B-3795
  17. Sharma A., Dutt G., Jayakumar S., Saroha V., Singh M. K., Pathodiya O. P., Khaddae B. S., Dixit S. P. (2013) . Novel SNPs in IGF-1, GHR and IGFBP-3 genes reveal significant association with growth traits in Indian goat breeds. Small Ruminant Research. 115: 7-14.
  18. Wang, W.J., Ouyang, K., Ouyang, J., Li, H.H., Lin, S.M., Sun, H. (2004). Polymorphism of insulin-like growth factor I gene in six chicken breeds and its relationship with growth traits. Asian-Australasian Journal of Animal Sciences. 17: 301-304.
  19. Wu-Jun, L., Guang-Xin, F., Yi, F., Ke-Chuan, T., Xi-Xia, H., Xin-Kui, Y., Mou, W., Hui, Y., et al., (2010). The polymorphism of a mutation of IGF-1 gene on two goat breeds in China. J Anim Vet Adv. 9(4): 790-794.
  20. Yakar ,S., Rosen, C.J., Beamer, W.G., Ackert-Bicknell, C.L., Wu, Y., Liu, J.L., Ooi, G.T., Setser, J., Frystyk, J., Boisclair, Y.R., LeRoith, D. (2002). Circulating levels of IGF-I directly regulate bone growth and density. Clinical Investigation. 110: 771-781.
  21. Zhang, C., Zhang, W., Luo, H., Yue, W., Gao M., Jia Z. (2008). A new single nucleotide polymorphism in the IGF-I gene and its association with growth traits in the Nanjiang Huang goat. Asian-Australasian Journal of Animal Sciences. 21(8):1073-1079.
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Indian Journal of Animal Research
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    Research Article

    volume 54 issue 11 (november 2020) : 1451-1454,   Doi: 10.18805/ijar.B-3893

    Study on Genetic Polymorphism of IGF-1 Gene in Barbari and Jamunapari Goat

    M
    Mahesh Shivanand Dige
    R
    Rakesh Kaushik
    B
    Bhavana Kushwah
    M
    M.K. Singh
    S
    Saket Bhusan
    P
    P.K. Rout
    1Animal Genetics and Breeding Division, ICAR-CIRG, Makhdoom, Mathura-281 122, Uttar Pradesh, India. 
    Cite article:- Dige Shivanand Mahesh, Kaushik Rakesh, Kushwah Bhavana, Singh M.K., Bhusan Saket, Rout P.K. (2020). Study on Genetic Polymorphism of IGF-1 Gene in Barbari and Jamunapari Goat. Indian Journal of Animal Research. 54(11): 1451-1454. doi: 10.18805/ijar.B-3893.

    ABSTRACT

    Insulin like growth factor (IGF 1) play an important role in the production and reproduction. One hundred and ninety two adult goats of Barbari and Jamunapari breed were taken for study. Data on growth traits were recorded to identify SNPs in the IGF-1gene by using High Resolution Melting (HRM) technique. Five fragments of IGF-1 were amplified length of amplicon were 105, 124, 150, 122 and 122 bp covering 5’UTR, 3’UTR, Exon 3, Exon 2 and 5’UTR regions, respectively and subsequently sequenced. The HRM analysis revealed no sequence variation among the samples of both breeds. Hence, the all five amplified fragments of IGF-1 gene showed monomorphic pattern and, no significant association could be detected with growth traits in Barbari and Jamunapari goats.

    REFERENCES

    1. Brisson, B.K., Barton, E.R. (2012). Insulin-like growth factor-I E-    peptide activity is dependent on the IGF-I receptor. PLoS One. 7(9): e45588.
    2. Curi, R.A., Oliveira, H.N., Silveira, A.C., Lopes, C.R. (2005). Association between IGF-I, IGF-IR and GHRH gene polymorphisms and growth and carcass traits in beef cattle. Livestock Production Science. 94: 159-167.
    3. Darwish, H.R, El-Shorbagy, H.M, Abdou-Eisha, A.M, El-Din, A.E and Farag, I.M. (2017).New Polymorphism in the 5’ flanking region of IGF1 gene and its association with wool traits in Egyptian Barki Sheep. J Genet Engin Biotech. 15: 437-441.
    4. Daughaday, W.H., Rotwein, P. (1989). Insulin-like growth factors I and II. Peptide, messenger ribonucleic acid and gene structures, serum, and tissue concentrations. Endocrine Reviews. 10:68-91.
    5. Jensen, E. C., Harding, J. H., Bauer, M.K., Gluckman, P.D. (1999). Metabolic effects of IGF-I in the growth retarded fetal sheep. Journal of Endocrinology. 161: 485-494.
    6. Kaushik, R., Dige, M.S., Rout, P.K. (2016). Molecular characterization and expression profiling of ENOX2 gene in response to heat stress in goats. Cell and Development Biology. 5:2. DOI: 10.4172/2168-9296.1000176.
    7. Kaushik, R., Goel, A., Rout, P.K. (2019). Differential and characterization of ATP1A1 exon17 gene by high resolution melting analysis and RT PCR in Indian goats. Molecular Biology Reports. doi.org/10.1007/s11033-019-04984-4.
    8. Kurdistani, Z., Rostamzadeh, J., Rashidi, A., Davis, M.E. (2013). Evaluation of insulin-like growth factor-I gene polymorphism on growth traits and yearling fleece weight in goats. Small Ruminant Research. 111(1): 10–5.
    9. Li, C., Basarab, J., Snelling, W.M., Benkel, B., Murdoch, B., Hansen, C., Moore SS. (2004): Assessment of positional candidate genes myf5 and igf1 for growth on bovine chromosome 5 in commercial lines of Bostaurus. Journal of Animal Science. 82:1-7
    10. Moody, D.E., Pomp, D., Newman, S., Macneil, M.D. (1996). Characterization of DNA polymorphisms in three populations of Hereford cattle and their associations with growth and maternal EPD in line 1 Herefords. Journal of Animal Science. 74: 1784-1793. 
    11. Moradian C., Mohamadi N., Sheshdeh S.A.R., Hajihosseinlo A., Ashrafi F. (2013). Effects of genetic polymorphismat the growth hormone gene on growth traits in Makooei sheep. European Journal of Experimental Biology. 3(3): 101-105.
    12. Naicy, T., Venkatachalapathy, R.T., Aravindakshan, T.V., Kurian, E. (2017).Association of a Cac8I polymorphism in the IGF1 gene with growth traits in Indian goats. Journal of Genetic Engineering and Biotechnology. 15: 7–11. 
    13. Qiong, W., Chao, F., Wu-Jun, L., Yi, F., Shi-Gang, Y. (2011). A novel mutation at exon 4 of IGF-1 gene in three indigenous goat breed sin China. Asian Journal of Animal and Veterinary Advances. 6(6):627-635.
    14. Rasouli, S., Abdolmohammadi, A., Zebarjadi, A. and Mostafei, A., (2017). Evaluation of polymorphism in IGF-I and IGFBP-    3 genes and their relationship with twinning rate and growth traits in Markhoz goats. Ann Anim Sci. 17(1): 89-103.
    15. Rout, P.K., Saxena, V.K., Khan, B.U., Roy, R., Singh, S.K., Singh, L.B. (2000). Characterization of Jamunapari goats in their home tract. Anim Genet Resour Infor. 27:43.
    16. Sankhyan, V., Thakur, Y.P., Dogra, P.K., (2019). Genetic polymorphism in IGF-1 gene in four sheep and goat breeds and its association with biometrical traits in migratory Gaddi goat breed of western Himalayan state of Himachal Pradesh, India. Indian Journal of Animal Research. DOI: 10.18805/    ijar.B-3795
    17. Sharma A., Dutt G., Jayakumar S., Saroha V., Singh M. K., Pathodiya O. P., Khaddae B. S., Dixit S. P. (2013) . Novel SNPs in IGF-1, GHR and IGFBP-3 genes reveal significant association with growth traits in Indian goat breeds. Small Ruminant Research. 115: 7-14.
    18. Wang, W.J., Ouyang, K., Ouyang, J., Li, H.H., Lin, S.M., Sun, H. (2004). Polymorphism of insulin-like growth factor I gene in six chicken breeds and its relationship with growth traits. Asian-Australasian Journal of Animal Sciences. 17: 301-304.
    19. Wu-Jun, L., Guang-Xin, F., Yi, F., Ke-Chuan, T., Xi-Xia, H., Xin-Kui, Y., Mou, W., Hui, Y., et al., (2010). The polymorphism of a mutation of IGF-1 gene on two goat breeds in China. J Anim Vet Adv. 9(4): 790-794.
    20. Yakar ,S., Rosen, C.J., Beamer, W.G., Ackert-Bicknell, C.L., Wu, Y., Liu, J.L., Ooi, G.T., Setser, J., Frystyk, J., Boisclair, Y.R., LeRoith, D. (2002). Circulating levels of IGF-I directly regulate bone growth and density. Clinical Investigation. 110: 771-781.
    21. Zhang, C., Zhang, W., Luo, H., Yue, W., Gao M., Jia Z. (2008). A new single nucleotide polymorphism in the IGF-I gene and its association with growth traits in the Nanjiang Huang goat. Asian-Australasian Journal of Animal Sciences. 21(8):1073-1079.
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