Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 54 issue 7 (july 2020) : 900-904

Differential Expression of Cytokine in Kadaknath and Commercial Chicken Against Coccidial Challenge

M.S. Thakur, S.N.S. Parmar, Amit Kumar Jha, Akhilesh Pandey
1Department of Animal Genetics and Breeding, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.
Cite article:- Thakur M.S., Parmar S.N.S., Jha Kumar Amit, Pandey Akhilesh (2019). Differential Expression of Cytokine in Kadaknath and Commercial Chicken Against Coccidial Challenge. Indian Journal of Animal Research. 54(7): 900-904. doi: 10.18805/ijar.B-3851.
In the present investigation the relative expression of interleukins (IL)-1â, IL-2, IL-6, IL-17 and interferon (IFN)-Y genes were explored in response to coccidial challenge in Kadaknath (n=12), Cari-Vishal (n=12) and Cobb (n=12) broiler chicken using quantitative PCR. The intraepithelial lymphocytes cell of control, T1 and T2 on 4, 7 and 14d post infection (pi) were used as templates for expression of five inflammatory cytokines. The relative mean fold expression for ILâ1 ranged from 9.91± 0.14 (Kadaknath) to 119.01±0.29 (Cobb). The highest and lowest relative mean fold expression for IL-2 was observed to be 242.19±0.40 and 6.25±0.03 in Cobb at d4 and d14 pi, respectively. For IL-6 gene, the lowest relative mean fold expression was observed in Kadaknath (8.11±0.05) while the highest value was recorded in Cobb (99.04±0.92). The relative mean fold expression for IL-17 was 11.04±0.29 in Kadaknath and 197.40±0.37 in Cobb. For IFN-Y gene, the lowest relative mean fold expression was observed in Kadaknath (8.11±0.19), while the highest value was recorded in Cobb (84.74±0.25). In Cobb and Cari-Vishal broilers most cytokines (IL-1â, IL-6, IL-17 and IFN-Y) were observed to be transcribed to higher levels than Kadaknath chicken. It is concluded that the differential expression of cytokine genes in three genetic groups showed different degree of mucosal immune response to Eimeria infection and it depend upon the genetic background/genotype of birds, coccidial dosage and age of infection. 
  1. Al-Idreesi, S.R., Kweider, M., Katranji, M.M. (2013). Immune responses to Eimeria tenella sporozoite protein as vaccine to broiler against coccidiosis. International Journal of Poultry Science. 12:582-589.
  2. Gadde, U., Chapman, H.D., Rathinam, T., Erf, G.F. (2011). Cellular immune responses, chemokine and cytokine profiles in Turkey poults following infection with the intestinal parasite Eimeria adenoeides. Poultry Science. 90:2243-2250.
  3. Haritova, A.M. and Stanilova, S.A. (2012). Enhanced expression of IL-10 in contrast to IL-12B mRNA in poultry with experimental coccidiosis. Experimental Parasitology. 132:378-382. 
  4. Hong, Y.H., Lillehoj, H.S., Lee, S.H., Dalloul, R.A., Lillehoj, E.P. (2006). Analysis of chicken cytokine and chemokine gene expression following Eimeria acervulina and Eimeria tenella infections. Veterinary Immunology and Immunopathology. 114:209–223.
  5. Kim, W.H., Jeong, J., Park, A.R., Yim, D., Kim, Y.M., Kim, K.D., Chang, H.H., Lillehoj, H.S., Lee, B.H., Min, W. (2012). Chicken IL-    17F identification and comparative expression analysis in Eimeria infected chicken. Developmental and Comparative Immunology. 38:401-409.
  6. Livak, K.J. and Schmittgen, T.D. (2001). Analysis of relative gene expression data using Real Time Quantitative PCR and the 2-DDCT. Methods, 25:402–408.
  7. Rio, D.C., Ares, M., Hannon, G.C., Nilsen, T.W. (2010). Purification of RNA using TRIzol (TRI reagent). Cold Spring Harbor Protocols. 6: doi:10.1101/pdb.prot5439.
  8. Rothwell, L., Young, J.R., Zoorob, R., Whittaker, C.A., Hesketh, P., Archer, A., Smith, A.L., Kaiser, P. (2004). Cloning and characterization of chicken IL-10 and its role in the immune response to Eimeria maxima. Journal of Immunology. 173:2675-2682.
  9. Schat, K.A. and Davis, C. (1999). Resistance to viral disease. In: Breeding for disease resistance in farm animals, 2nd ed., [Axford, R.F.E., Bishop, S.C., Nicholas, F. and Owen (eds.)], CAB, Wallingford, United Kingdom, p 271-300.
  10. Wigley, P. and Kaiser, P. (2003). Avian cytokines in health and disease. Brazilian Journal of Poultry Science. 5:1-14
  11. Zacharias, D.A., Violin, J.D., Newton, A.C., Tsien, R.Y. (2002). Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science. 296:913-916.
  12. Zhang, L., Liu, R., Ma, L., Wang, Y., Pan, B., Cai, J., Wang, M. (2012). Eimeria tenella: expression profiling of toll-like receptors and associated cytokines in the caecum of infected day-old and three-week old SPF chickens. Experimental Parasitology. 130:442-448.

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