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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 54 issue 11 (november 2020) : 1428-1432,   Doi: 10.18805/ijar.B-3848

Isolation and Identification of Major Mastitis Causing Bacteria from Clinical Cases of Bovine Mastitis in Kashmir Valley

R
R. Waseem
A
A. Muhee
H
H.U. Malik
Z
Z.A. Akhoon
K
Khusheeba Munir
S
S.U. Nabi
S
Syed Taifa
1Clinical Veterinary Medicine SKUAST Kashmir.
Cite article:- Waseem R., Muhee A., Malik H.U., Akhoon Z.A., Munir Khusheeba, Nabi S.U., Taifa Syed (2020). Isolation and Identification of Major Mastitis Causing Bacteria from Clinical Cases of Bovine Mastitis in Kashmir Valley. Indian Journal of Animal Research. 54(11): 1428-1432. doi: 10.18805/ijar.B-3848.

ABSTRACT

Background: Mastitis is one of the most economically important diseases of dairy animals causing heavy losses in terms of fall in milk production, decreased milk quality and additional costs for veterinary services. Mastitis is caused by a wide variety of microorganisms including bacteria, fungi, yeast and mycoplasma, of which bacteria are the most frequently isolated pathogens. Microbiological testing is necessary for identification of cause of mastitis and adaptation of control measures to prevent the disease. The aim of the presnt study was to isolate and identify major mastitis causing bacteria from clinical mastitic cases presented to Veterinary Clinical complex, FVSc. & AH, Shuhama Kashmir for treatment. 
Methods: A total of 200 bovine milk samples from CMT confirmed clinical mastitis cases were collected and cultured on Nutrient agar, Blood agar, Mac-Conkey agar, Eosine Methylene Blue (EMB) agar, Mannitol salt agar and Edwards media for bacterial isolation. The isolates were then subjected to biochemical tests for identification. 
Result: Upon microbiological testing and biochemical confirmation, the major mastitis causing bacteria isolated from clinical cases of Bovine mastitis were Staphylococcus Spp. showing highest prevalence of 46.4% followed by mixed infection with Staphylococcus and Streptococcus (20.8%), Streptococci Spp. (18.4%) and Escherichia coli (14.4%). 

REFERENCES

  1. Ahmady, M. and Kazemi, S. (2013). Detection of the enterotoxigenic genes in Staphylococcus aureus isolates from bovine mastitis milk in the West Azerbaijan of Iran. Comparative Clinical Pathology. 22: 649-654.
  2. Bartlett, P.P., Miller, G.Y., Lance, S.E. and Lawrance, E.H. (1992). Clinical mastitis and intramammary infection on Ohio dairy farms. Preventive Veterinary Medicine. 12: 59-71.
  3. Bhat, A.M., Soodan, J.S., Singh, R., Dhobi, I. A., Hussain, T., Dar, M.Y. and Mir, M. (2017). Incidence of bovine Clinical mastitis in Jammu region and antibiogram of isolated pathogens. Veterinary World. 10(8): 211-218.
  4. Bhatt, V.D., Patel, M.S., Joshi, C.G. and Kunjadia, A. (2011). Identification and antibiogram of microbes associated with bovine mastitis. Animal Biotechnology. 22:163-169.
  5. Boscos, C., Stefanakis, A., Alexopoulos, C and Samartzi, F. (1996). Prevalence of subclinical mastitis and influence of breed, parity, stage of lactation and mammary bacteriological status of Counter Counts and California Mastitis Test in the milk of Saanen and auto-chthonous Greek goats. Small Ruminant Research. 21: 139-147. 
  6. Bradley, A.J. (2002). Bovine Mastitis: an Evolving disease. Veterinary Journal. 163: 1-13.
  7. Halasa T, K Huijps, O Osteras and H Hogeveen. (2007). Economic effects of bovine mastitis and mastitis management: a review: Veterinary Querterly. 29: 18-31.
  8. Hillerton, J.E., Bramley, A.J., Staker, R.T. and McKinnon. (1995). C.H. Patterns of intramammary infection and clinical mastitis over a 5 year period in a closely monitored herd applying mastitis control measure. Journal of Dairy Research. 62: 39-50.
  9. Holdaway, R.J., Holmes, C.W. and Steffert, I.J. (1996). A comparison of indirect methods for diagnosis of subclinical intramammary infection in lactating dairy cows. Australian Journal of Dairy Technology. 51: 64-71.
  10. Hortet, P. and Seegers, H. (1998). Loss in milk yield and related composition changes resulting from clinical mastitis in dairy cows. Preventive Veterinary Medicine. 37: 1-20.
  11. Keane, O.M., Budd, K.E., Flynnand, J and McCoy, F. (2013). Pathogen profile of clinical mastitis in Irish milk-recording herds reveals a complex etiology. Veterinary Record. 173(1): 17. doi: 10.1136/vr. 101308.
  12. Mallikarjunaswamy, M.C. and Murthy, G.V.K. (1997). Antibiogram of bacterial pathogens isolated from bovine subclinical mastitis cases. Indian Veterinary Journal. 74: 885-886.
  13. Pati, B.K. and Mukherjee, R. (2016). Characterization of Staphylococcus aureus isolates of Bovine Mastitis Origin and antibiotic Sensitivity Pattern from Northern Plains of India. Journal of Veterinary Research and Animal Husbandry. 1(1): 105.
  14. Radostits, O.M., C.C. Gay, D.C. Blood and K.W. Hinchlif, (2007). Veterinary Medicine 9 Ed., Harcourt Ltd and London, Pp: 174-758.
  15. Raemy, A., Meylan, M., Casati, S., Gaia, V., Berchtold, B., Boss, R., Wyderand, A. and Graber, H.U. (2013). Phenotypic and genotypic identification of streptococci and related bacteria isolated from bovine intramammary infections. Acta Veterinaria Scandinavica. 55: 1-9.
  16. Sears, P.M., Gonzalez, R.N., Wilson, D.J. and Han, H.R. (1993). Procedures for mastitis diagnosis and control. The Veterinary Clinics of North America (Food Animal Practice). 9: 445-468.
  17. Shaheen, M., Tantary, H. A. and Nabi, S. U. (2016). A Treatise on Bovine Mastitis: Disease and Disease Economics, Etiological Basis, Risk Factors, Impact on Human Health, Therapeutic Management, Prevention and Control Strategy. Journal of Advances in Dairy Research. 4: 15.
  18. Sumathi, B.R., Veeregowda, B.M. and Amitha, R.G. (2008). Prevalence and antibiogram profile of bacterial isolates from clinical bovine mastitis. Veterinary World. 1(8): 237-238.
  19. Waage, S., Mork, T., Roros, T., Aasland, D., Hunshamar, A. and Odegaard, S.A. (1999). Bacteria associated with Clinical mastitis in dairy heifers. Journal of Dairy Science. 82(4): 712-9.
  20. Watts, J.L. (1990). Bovine mastitis. In: Carter, G.R.; Cole, Jr., J.R. (Eds.). Diagnostic Procedures in Veterinary Bacteriology and Mycology. 5th edition Academic Press, Inc., SanDiego, California.
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    Research Article

    volume 54 issue 11 (november 2020) : 1428-1432,   Doi: 10.18805/ijar.B-3848

    Isolation and Identification of Major Mastitis Causing Bacteria from Clinical Cases of Bovine Mastitis in Kashmir Valley

    R
    R. Waseem
    A
    A. Muhee
    H
    H.U. Malik
    Z
    Z.A. Akhoon
    K
    Khusheeba Munir
    S
    S.U. Nabi
    S
    Syed Taifa
    1Clinical Veterinary Medicine SKUAST Kashmir.
    Cite article:- Waseem R., Muhee A., Malik H.U., Akhoon Z.A., Munir Khusheeba, Nabi S.U., Taifa Syed (2020). Isolation and Identification of Major Mastitis Causing Bacteria from Clinical Cases of Bovine Mastitis in Kashmir Valley. Indian Journal of Animal Research. 54(11): 1428-1432. doi: 10.18805/ijar.B-3848.

    ABSTRACT

    Background: Mastitis is one of the most economically important diseases of dairy animals causing heavy losses in terms of fall in milk production, decreased milk quality and additional costs for veterinary services. Mastitis is caused by a wide variety of microorganisms including bacteria, fungi, yeast and mycoplasma, of which bacteria are the most frequently isolated pathogens. Microbiological testing is necessary for identification of cause of mastitis and adaptation of control measures to prevent the disease. The aim of the presnt study was to isolate and identify major mastitis causing bacteria from clinical mastitic cases presented to Veterinary Clinical complex, FVSc. & AH, Shuhama Kashmir for treatment. 
    Methods: A total of 200 bovine milk samples from CMT confirmed clinical mastitis cases were collected and cultured on Nutrient agar, Blood agar, Mac-Conkey agar, Eosine Methylene Blue (EMB) agar, Mannitol salt agar and Edwards media for bacterial isolation. The isolates were then subjected to biochemical tests for identification. 
    Result: Upon microbiological testing and biochemical confirmation, the major mastitis causing bacteria isolated from clinical cases of Bovine mastitis were Staphylococcus Spp. showing highest prevalence of 46.4% followed by mixed infection with Staphylococcus and Streptococcus (20.8%), Streptococci Spp. (18.4%) and Escherichia coli (14.4%). 

    REFERENCES

    1. Ahmady, M. and Kazemi, S. (2013). Detection of the enterotoxigenic genes in Staphylococcus aureus isolates from bovine mastitis milk in the West Azerbaijan of Iran. Comparative Clinical Pathology. 22: 649-654.
    2. Bartlett, P.P., Miller, G.Y., Lance, S.E. and Lawrance, E.H. (1992). Clinical mastitis and intramammary infection on Ohio dairy farms. Preventive Veterinary Medicine. 12: 59-71.
    3. Bhat, A.M., Soodan, J.S., Singh, R., Dhobi, I. A., Hussain, T., Dar, M.Y. and Mir, M. (2017). Incidence of bovine Clinical mastitis in Jammu region and antibiogram of isolated pathogens. Veterinary World. 10(8): 211-218.
    4. Bhatt, V.D., Patel, M.S., Joshi, C.G. and Kunjadia, A. (2011). Identification and antibiogram of microbes associated with bovine mastitis. Animal Biotechnology. 22:163-169.
    5. Boscos, C., Stefanakis, A., Alexopoulos, C and Samartzi, F. (1996). Prevalence of subclinical mastitis and influence of breed, parity, stage of lactation and mammary bacteriological status of Counter Counts and California Mastitis Test in the milk of Saanen and auto-chthonous Greek goats. Small Ruminant Research. 21: 139-147. 
    6. Bradley, A.J. (2002). Bovine Mastitis: an Evolving disease. Veterinary Journal. 163: 1-13.
    7. Halasa T, K Huijps, O Osteras and H Hogeveen. (2007). Economic effects of bovine mastitis and mastitis management: a review: Veterinary Querterly. 29: 18-31.
    8. Hillerton, J.E., Bramley, A.J., Staker, R.T. and McKinnon. (1995). C.H. Patterns of intramammary infection and clinical mastitis over a 5 year period in a closely monitored herd applying mastitis control measure. Journal of Dairy Research. 62: 39-50.
    9. Holdaway, R.J., Holmes, C.W. and Steffert, I.J. (1996). A comparison of indirect methods for diagnosis of subclinical intramammary infection in lactating dairy cows. Australian Journal of Dairy Technology. 51: 64-71.
    10. Hortet, P. and Seegers, H. (1998). Loss in milk yield and related composition changes resulting from clinical mastitis in dairy cows. Preventive Veterinary Medicine. 37: 1-20.
    11. Keane, O.M., Budd, K.E., Flynnand, J and McCoy, F. (2013). Pathogen profile of clinical mastitis in Irish milk-recording herds reveals a complex etiology. Veterinary Record. 173(1): 17. doi: 10.1136/vr. 101308.
    12. Mallikarjunaswamy, M.C. and Murthy, G.V.K. (1997). Antibiogram of bacterial pathogens isolated from bovine subclinical mastitis cases. Indian Veterinary Journal. 74: 885-886.
    13. Pati, B.K. and Mukherjee, R. (2016). Characterization of Staphylococcus aureus isolates of Bovine Mastitis Origin and antibiotic Sensitivity Pattern from Northern Plains of India. Journal of Veterinary Research and Animal Husbandry. 1(1): 105.
    14. Radostits, O.M., C.C. Gay, D.C. Blood and K.W. Hinchlif, (2007). Veterinary Medicine 9 Ed., Harcourt Ltd and London, Pp: 174-758.
    15. Raemy, A., Meylan, M., Casati, S., Gaia, V., Berchtold, B., Boss, R., Wyderand, A. and Graber, H.U. (2013). Phenotypic and genotypic identification of streptococci and related bacteria isolated from bovine intramammary infections. Acta Veterinaria Scandinavica. 55: 1-9.
    16. Sears, P.M., Gonzalez, R.N., Wilson, D.J. and Han, H.R. (1993). Procedures for mastitis diagnosis and control. The Veterinary Clinics of North America (Food Animal Practice). 9: 445-468.
    17. Shaheen, M., Tantary, H. A. and Nabi, S. U. (2016). A Treatise on Bovine Mastitis: Disease and Disease Economics, Etiological Basis, Risk Factors, Impact on Human Health, Therapeutic Management, Prevention and Control Strategy. Journal of Advances in Dairy Research. 4: 15.
    18. Sumathi, B.R., Veeregowda, B.M. and Amitha, R.G. (2008). Prevalence and antibiogram profile of bacterial isolates from clinical bovine mastitis. Veterinary World. 1(8): 237-238.
    19. Waage, S., Mork, T., Roros, T., Aasland, D., Hunshamar, A. and Odegaard, S.A. (1999). Bacteria associated with Clinical mastitis in dairy heifers. Journal of Dairy Science. 82(4): 712-9.
    20. Watts, J.L. (1990). Bovine mastitis. In: Carter, G.R.; Cole, Jr., J.R. (Eds.). Diagnostic Procedures in Veterinary Bacteriology and Mycology. 5th edition Academic Press, Inc., SanDiego, California.
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