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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 11 (november 2019) : 1436-1439,   Doi: 10.18805/ijar.B-3698

Expression of Stearoyl Co-enzyme A desaturase (SCD) gene during the juvenile age in layer chicken

N
N. Govardhana Sagar
T
T.K. Bhattacharya
A
A. Rajendra Prasad
P
Pushpendra Kumar
R
R.N.Chatterjee
B
Bharat Bhushan
K
K.K Kanaka
1Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, Uttar Pradesh, India.
Cite article:- Sagar Govardhana N., Bhattacharya T.K., Prasad Rajendra A., Kumar Pushpendra, R.N.Chatterjee, Bhushan Bharat, Kanaka K.K (2019). Expression of Stearoyl Co-enzyme A desaturase (SCD) gene during the juvenile age in layer chicken. Indian Journal of Animal Research. 53(11): 1436-1439. doi: 10.18805/ijar.B-3698.

ABSTRACT

Stearoyl-CoA desaturase (SCD) is key enzyme in the production of triglycerides and cholesterol. Present study was conducted to know the expression pattern of the Stearoyl-CoA desaturase (SCD) gene in liver during juvenile age (0 day, 14th day, 28th day and 42nd day) in layer birds. Expression of SCD gene increased from the day of hatch to 42nd day, as the body weight and fat deposition increased. The mRNA expression levels of SCD gene during day 0 was differed significantly (P<0.05) with expression levels during other juvenile ages. When compared with SCD expression levels on the day 0 expression levels were up-regulated by 8198.64, 7245.99 and 5587.77 folds over day 14,  28 and  42 respectively. Serum concentration of triglycerides and cholesterol were found to be proportional to the expression levels of SCD gene in liver. It is concluded that SCD expression varied among different age groups during juvenile stages and had significant association with serum triglycerides and cholesterol concentration in layer chicken.

REFERENCES

  1. Bai, S., Wang, G., Zhang, W., Zhang, S., Rice, B. B., Cline, M. A. and Gilbert, E.R. (2015). Broiler chicken adipose tissue dynamics during the first two weeks post hatch. Comp. Biochem. and Physiol. Part A: Mol. and Int. Phy., 189:115- 123.
  2. Bhattacharya, T.K. and Chatterjee, R.N. (2013). Polymorphism of the myostatin gene and its association with growth traits in chicken. Poultry Science, 92: 910-915.
  3. Bhattacharya, T.K., Chatterjee, R.N., Raj Kumar, U., Rajaravindra, K.S. and Niranjan, M. (2014). Genetic polymorphism in the promoter of prolactin gene in layer chicken. Indian Journal of Animal Research, 49: 875-877. 
  4. Chomczynski, P., and Sacchi, N. (2006). The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nature Protocols, 1:581. 
  5. Diot, C., Lefèvre, P., Hervé, C., Belloir, B., Narce, M., Damon, M., Mallard J., and Douaire, M. (2000). Stearoyl-CoA desaturase 1 coding sequences and antisense RNA affect lipid secretion in transfected chicken LMH hepatoma cells. Archives of biochemistry and biophysics, 380:243-250. 
  6. Douaire, M., Le Fur, N., el Khadir-Mounier, C., Langlois, P., Flamant, F., and Mallard, J. (1992). Identifying genes involved in the variability of genetic fatness in the growing chicken. Poultry science, 71:1911-1920. 
  7. DPR Annual Report 2017-18. Published by ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad.
  8. Griffin, H. D., Guo, K., Windsor, D., and Butterwith, S. C. (1992). Adipose tissue lipogenesis and fat deposition in leaner broiler chickens. The Journal of nutrition, 122:363-368. 
  9. Leclercq, B., Blum, J. C., and Boyer, J. P. (1980). Selecting broilers for low or high abdominal fat: initial observations. British Poultry Science, 21:107-113. 
  10. Legrand, P., Catheline, D., Fichot, M. C., and Lemarchal, P. (1997). Inhibiting Ä9-desaturase activity impairs triacylglycerol secretion in cultured chicken hepatocytes. The Journal of nutrition, 127:249-256. 
  11. Livak, K. J., and Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2" ÄÄCT method. methods, 25:402-408. 
  12. Miyazaki, M., and Ntambi, J. M. (2003). Role of stearoyl-coenzyme A desaturase in lipid metabolism. Prostaglandins, Leukotrienes and Essential Fatty Acids, 68:113-121.
  13. Miyazaki, M., Kim, Y. C., Gray-Keller, M. P., Attie, A. D., and Ntambi, J. M. (2000). The biosynthesis of hepatic cholesterol esters and triglycerides is impaired in mice with a disruption of the gene for stearoyl-CoA desaturase 1. Journal of Biological Chemistry, 275:30132-30138.
  14. Paswan, C., Bhattacharya, T.K., Chatterjee, R.N., nagaraja, C.S. and Dushyanth, K. (2016). Molecular characterization of promoter of IGF-1 gene in chicken broiler line and its growth performance. Indian Journal of Animal Research, 50: 23-26.
  15. Pearce, J. (1977). Effects of testosterone on hepatic lipid metabolism in the mature female domestic fowl. Journal of Endocrinology, 75:343-344. 
  16. Phillips, J., G., Butler, P., J., Sharp, P., J. (1985). Physiological strategy in avian biology: the reproductive system and its function. PP. 13.
  17. Pizer, E.S., Kurman, R.J., Pasternack, G.R. and Kuhajda, F.P. (1997). Expression of fatty acid synthase is closely linked to proliferation and stromal decidualization in cycling endometrium. International Journal of Gynecological Pathology: Official Journal of the International Society of Gynecological Pathologists, 16:45-51.
  18. Whitehead, C. C. and Griffin, H. D. (1984). Development of divergent lines of lean and fat broilers using plasma very low density lipoprotein concentration as selection criterion: the first three generations. British Poultry Science, 25:573-582. 
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    Research Article

    volume 53 issue 11 (november 2019) : 1436-1439,   Doi: 10.18805/ijar.B-3698

    Expression of Stearoyl Co-enzyme A desaturase (SCD) gene during the juvenile age in layer chicken

    N
    N. Govardhana Sagar
    T
    T.K. Bhattacharya
    A
    A. Rajendra Prasad
    P
    Pushpendra Kumar
    R
    R.N.Chatterjee
    B
    Bharat Bhushan
    K
    K.K Kanaka
    1Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly-243 122, Uttar Pradesh, India.
    Cite article:- Sagar Govardhana N., Bhattacharya T.K., Prasad Rajendra A., Kumar Pushpendra, R.N.Chatterjee, Bhushan Bharat, Kanaka K.K (2019). Expression of Stearoyl Co-enzyme A desaturase (SCD) gene during the juvenile age in layer chicken. Indian Journal of Animal Research. 53(11): 1436-1439. doi: 10.18805/ijar.B-3698.

    ABSTRACT

    Stearoyl-CoA desaturase (SCD) is key enzyme in the production of triglycerides and cholesterol. Present study was conducted to know the expression pattern of the Stearoyl-CoA desaturase (SCD) gene in liver during juvenile age (0 day, 14th day, 28th day and 42nd day) in layer birds. Expression of SCD gene increased from the day of hatch to 42nd day, as the body weight and fat deposition increased. The mRNA expression levels of SCD gene during day 0 was differed significantly (P<0.05) with expression levels during other juvenile ages. When compared with SCD expression levels on the day 0 expression levels were up-regulated by 8198.64, 7245.99 and 5587.77 folds over day 14,  28 and  42 respectively. Serum concentration of triglycerides and cholesterol were found to be proportional to the expression levels of SCD gene in liver. It is concluded that SCD expression varied among different age groups during juvenile stages and had significant association with serum triglycerides and cholesterol concentration in layer chicken.

    REFERENCES

    1. Bai, S., Wang, G., Zhang, W., Zhang, S., Rice, B. B., Cline, M. A. and Gilbert, E.R. (2015). Broiler chicken adipose tissue dynamics during the first two weeks post hatch. Comp. Biochem. and Physiol. Part A: Mol. and Int. Phy., 189:115- 123.
    2. Bhattacharya, T.K. and Chatterjee, R.N. (2013). Polymorphism of the myostatin gene and its association with growth traits in chicken. Poultry Science, 92: 910-915.
    3. Bhattacharya, T.K., Chatterjee, R.N., Raj Kumar, U., Rajaravindra, K.S. and Niranjan, M. (2014). Genetic polymorphism in the promoter of prolactin gene in layer chicken. Indian Journal of Animal Research, 49: 875-877. 
    4. Chomczynski, P., and Sacchi, N. (2006). The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on. Nature Protocols, 1:581. 
    5. Diot, C., Lefèvre, P., Hervé, C., Belloir, B., Narce, M., Damon, M., Mallard J., and Douaire, M. (2000). Stearoyl-CoA desaturase 1 coding sequences and antisense RNA affect lipid secretion in transfected chicken LMH hepatoma cells. Archives of biochemistry and biophysics, 380:243-250. 
    6. Douaire, M., Le Fur, N., el Khadir-Mounier, C., Langlois, P., Flamant, F., and Mallard, J. (1992). Identifying genes involved in the variability of genetic fatness in the growing chicken. Poultry science, 71:1911-1920. 
    7. DPR Annual Report 2017-18. Published by ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad.
    8. Griffin, H. D., Guo, K., Windsor, D., and Butterwith, S. C. (1992). Adipose tissue lipogenesis and fat deposition in leaner broiler chickens. The Journal of nutrition, 122:363-368. 
    9. Leclercq, B., Blum, J. C., and Boyer, J. P. (1980). Selecting broilers for low or high abdominal fat: initial observations. British Poultry Science, 21:107-113. 
    10. Legrand, P., Catheline, D., Fichot, M. C., and Lemarchal, P. (1997). Inhibiting Ä9-desaturase activity impairs triacylglycerol secretion in cultured chicken hepatocytes. The Journal of nutrition, 127:249-256. 
    11. Livak, K. J., and Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2" ÄÄCT method. methods, 25:402-408. 
    12. Miyazaki, M., and Ntambi, J. M. (2003). Role of stearoyl-coenzyme A desaturase in lipid metabolism. Prostaglandins, Leukotrienes and Essential Fatty Acids, 68:113-121.
    13. Miyazaki, M., Kim, Y. C., Gray-Keller, M. P., Attie, A. D., and Ntambi, J. M. (2000). The biosynthesis of hepatic cholesterol esters and triglycerides is impaired in mice with a disruption of the gene for stearoyl-CoA desaturase 1. Journal of Biological Chemistry, 275:30132-30138.
    14. Paswan, C., Bhattacharya, T.K., Chatterjee, R.N., nagaraja, C.S. and Dushyanth, K. (2016). Molecular characterization of promoter of IGF-1 gene in chicken broiler line and its growth performance. Indian Journal of Animal Research, 50: 23-26.
    15. Pearce, J. (1977). Effects of testosterone on hepatic lipid metabolism in the mature female domestic fowl. Journal of Endocrinology, 75:343-344. 
    16. Phillips, J., G., Butler, P., J., Sharp, P., J. (1985). Physiological strategy in avian biology: the reproductive system and its function. PP. 13.
    17. Pizer, E.S., Kurman, R.J., Pasternack, G.R. and Kuhajda, F.P. (1997). Expression of fatty acid synthase is closely linked to proliferation and stromal decidualization in cycling endometrium. International Journal of Gynecological Pathology: Official Journal of the International Society of Gynecological Pathologists, 16:45-51.
    18. Whitehead, C. C. and Griffin, H. D. (1984). Development of divergent lines of lean and fat broilers using plasma very low density lipoprotein concentration as selection criterion: the first three generations. British Poultry Science, 25:573-582. 
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