Immunohistochemical detection of alpha-smooth muscle actin and S-100 in bovine mammary gland with mastitis

DOI: 10.18805/ijar.B-3674    | Article Id: B-3674 | Page : 1440-1444
Citation :- Immunohistochemical detection of alpha-smooth muscle actin and S-100 in bovine mammary gland with mastitis.Indian Journal Of Animal Research.2019.(53):1440-1444
Geeta Devi Leishangthem, Nittin Dev Singh and Amarjit Singh drgeetapatho@gmail.com
Address : Animal Disease Research Centre, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.
Submitted Date : 28-06-2018
Accepted Date : 20-02-2019

Abstract

Fibrosis is a common finding in chronic mastitis. Epithelial-mesenchymal transition (EMT) plays a prominent role in fibrogenesis in adult tissues. The aim of this study was to investigate whether EMT occurs in bovine mastitis by assessing the expression or localization of alpha-smooth muscle actin (alpha-SMA), S-100 protein and caspase-3. Two normal bovine mammary gland tissue samples and five mastitic samples were used in the study. Antibodies to alpha-SMA, S-100 and caspase-3 were used for immunohistochemical studies. In mastitis there was increased expression of S-100 in the alveolar epithelial cells. Alpha-SMA was highly expressed in the areas where there is fibrosis in chronic mastitis. Further caspase-3 was observed in alveolar cells in mastitic tissue. Overexpression of S-100 and alpha-SMA directly correlates to the severity of mastitis.S-100 and alpha-SMA may serve as biomarkers to know the extent of tissue structural changes in mastitis. EMT could be one of the mechanistic pathways underlying the pathogenesis of fibrosis in mastitis. 

Keywords

Alpha-smooth muscle actin Bovine mastitis Caspase Epithelial to mesenchymal transition S-100.

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