Impairment induced due to oganophosphate compounds in some blood biochemical indices of fingerlings of Cyprinus carpio var. communis 

DOI: 10.18805/ijar.B-3662    | Article Id: B-3662 | Page : 1326-1328
Citation :- Impairment induced due to oganophosphate compounds in some blood biochemical indices of fingerlings of Cyprinus carpio var. communis.Indian Journal Of Animal Research.2019.(53):1326-1328
Imtiyaz Qayoom, Masood H. Balkhi, Feroz A. Shah and Bilal A. Bhat drimtiyazqayoom@rediffmail.com
Address : Division of Aquatic Environmental Management, Faculty of Fisheries, SKUAST–K, Rangil Ganderbal Jammu and Kashmir-190 006, India.
Submitted Date : 11-06-2018
Accepted Date : 1-11-2018

Abstract

Present study was aimed to investigate the effects of two organophosphate pesticides (dimethoate and chlorpyrifos) on some blood biochemical parameters of juveniles common carps (Cyprinus carpio var. communis). Fingerlings were subjected to bioassays divided into 5 experimental groups in range finding test on the basis of which the doses for definitive tests were selected. The acute bioassay toxicity tests were carried out in triplicates for 96 hours with a control group run parallel to the experiment. Data obtained was analyzed as per Finney’s probit to determine LC50 values. The mean lethal concentration (LC50) values for dimethoate and chlorpyrifos were found 1.1ppm and 3.8ppb respectively. The samples were investigated for various blood biochemical indices such as total protein (TP), serum albumin (SA), serum globulin (SGLO), alanine aminotransferase (ALT), asphartate aminotransferase (AAT), serum bilirubin (SB), serum glucose (SGLU), serum cholesterol (SC), and lactate dehydrogenase (LDH). All the liver enzymes (AAT, ALT, SB & LDH) and glucose (SGLU) were found increased while as TP, SA, SGLO, SB and SC were found decreased than the normal. Chlorpyrifos, was however, found to alter the biochemical indices more profoundly than dimethoate. The results indicated the organophosphate pesticides are intensely harmful for fish health. Their entry to natural waterbodies must be stopped besides ensuring their judicious application around the catchment area.

Keywords

Biochemistry Chlorpyrifos Dimethoate Fish LC50 Toxicity

References

  1. Abdel-Megeed, A. and Aly El-Nakieb, F. (2008). Dimethoate Degradtion by EM. Terrestrial and Aquatic Environmental Toxicology 2 (1), 1-4.
  2. Adoni, A.D., Joshi, G., Ghosh, K., Chourasia, S.K., Vaishya, A.K., Yadav, M. and Verma, H.G. 1985. Workbook on limnology. Department of Botany, Dr. Hari Singh Gour Vishwadidyalaya, Sagr Publishers, Sagar Madhya Pradesh, pp 26.
  3. APHA (2005). Standards Methods for the Examination of Water and Waste Water. 21stEdn., Washington D.C.
  4. Armstrong, D.T. (1968). Gonadotropins, ovarian metabolism and steroid biosynthesis. Rec. Prog. Horm. Res. 24: 255-319.
  5. Campagna, A.F., Eler, M.N., Espíndola, E.L.G., Senhorini, J.A., doRêgo, R.F.and Silva, L.O.L. (2004). Dimethoate 40% Organophosphorous Pesticide toxicity in Prochilodus Lineatus (Prochilodontidae, Characiformes) eggs and larvae. Brazilian Journal of Biology 66(2B): 633-640.
  6. Extoxnet, (1996). Extension Toxicology Network Pesticide Information Profiles. http://extoxnet.orst.edu/pips/chlorpyr.htm.
  7. Ferrando, M.D. and Andreu-Moliner, E. (1991). Effect of lindane on the blood of a freshwater fish. Bull. Environ. Contam. Toxicol. 47 : 465-470 .
  8. Finney, D.T. (1971). Probit Analysis; Cambridge University Press: Cambridge, UK.
  9. Ganeshwade, R.M. (2012). Biochemical changes induced by dimethoate (Rogor 30%EC) in the gills of fresh water fish Puntius ticto (Hamilton). Journal of Recent Trends in Bioscience, 2: 58-64.
  10. Ganeshwade, R.M., Rokade, P.B. and Sonwane, S.R. (2012). Impact of dimethoate on protein content in the freshwater fish Puntius ticto (HAM). TheBioscan. 7(1): 153 155.
  11. Hudgson, E. 2004. A Text Book of Modern Toxicology (3rd edn.). Department of Environmental and Biochemical Toxicology, North California State University, California, USA pp 288.
  12. Jagadeesan, Y. and Darcus, S. (2012). Insecticide (profenofos) induced biochemical changes in the fresh water fish Catla catla. Short Communication. International Journal of Current Science 20:120-124 pp.
  13. Qayoom, I. (2015). Residue analysis of pesticides in Dal waters and their effect on common carp fish, Cyprinus carpio var. communis. Thesis submitted to Sher e Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar J&K India. Pp 122-150.
  14. Qayoom, I., Balkhi, M.H., Mukhtar, M., Bhat, F.A., Shah, F.A. (2014). Biochemical toxicity of organophosphate compounds in Fishes. SKUAST Journal of Research 16: 1-13.
  15. Rahman, M. Z., Hossain, Z. M.F., Mollah, A. and Ahmed, G. U. (2002). Effect of eiazinon 60 EC on Anabas testudineus, Channa punctatus and Barbodes gonionotus. NAGA, The ICLARM Quarterly. 25(2): 8-12.
  16. Ramesh, M. and Sarvanan, M. (2008). Haematological and biochemical responses in a freshwater fish Cyprinus carpio exposed to Chlorpyrifos. International Journal of Integrative Biology 3: 82-85.
  17. Rao, J.V., Begum, G., Sridhar, V. and Reddy, N.C. (2005). Sub-lethal effects of monocrotophos on locomotor behaviour and gill architecture of the mosquito fish, Gambusia affinis. Journal of Environmental Science and Health, 40: 813-825.
  18. Reddy, P.K. and Leatherland, J.F. (1998). Stress Physiology. In: Fish Disease and Disorders (Eds. J.F. Leatherland and P.T.K. Woo), vol. 2, Non-infections Disorders, 1st edition. CABI Publishing, New York, pp 279-301.
  19. Reish, D.J., Oshida, R.S. (1987). Manual of Methods in Aquatic Environment Research. Part 10 short term static Bioassay. FAO Fish Tech. 247: 62.
  20. Remia, K.M., Logaswamy, S., Logankumar, K. and Rajmohan, D. (2008). Effect of an Insecticide (Monocrotophos) on some Biochemical constituents of the fish Tilapia mossambica. Pollution Res. 27(3): 523-526.
  21. Sen, S. and Bhattacharya, S. (1981). Role of thyroxin and Gonadotropins in the mobilization of ovarian cholesterol in a teleost Anabas testudineus (Bloch). Indian. J. Exp. Biol. 19: 408-412.
  22. Shamoushaki, M. N. M., Soltani, M., Kamali, A., Imanpoor, M. R., Sharifpour, I. and Khara, H. (2012). Effects of organophosphate, diazinon on some haematological and biochemical changes in Rutilus frisii kutum (Kamensky, 1901) male brood stocks. Iranian Journal of Fisheries Sciences11(1) 105-117 2012.
  23. Tilak, K.S., Wilson Raju, P. and Butchiram, M.S. (2009). Effects of alachlor on biochemical parameters of the freshwater fish, Channa punctatus (Bloch). Journal of Environmental Biology, 30: 421-426.
  24. Tripathi, P.K., Srivastava, V.K. and Singh, A. (2003). Toxic effects of Dimethoate (organophosphate) on metabolism and enzyme system of freshwater teleost fish Channa punctatus. Asian Fisheries Science 16: 349-359.
  25. Venkataraman, G.V., Rani, P.N. and Murthy, P.S. (2006). Impact of malathion on the biochemical parameters of gobid fish, Glossogobius giuris (Ham). Journal of Environmental Biology, 27: 199-212.
  26. Wells, R.M.G., Mcintyre, R.H., Morgan, A.K. and Davie, P.S. (1986). Physiological stress responses in big gamefish after capture: Observation on plasma chemistry and blood factors. Comparative Biochemistry and Physiology, 84A: 565-571.

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