Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 9 (september 2019) : 1243-1245,   Doi: 10.18805/ijar.B-3640

Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken

P
P. Ponnusamy
T
T. Lurthu Reetha
M
M. Sasikala
B
B.S.M. Ronald
J
J. Selvaraj
B
B. Puvarajan
R
R. Manickam
1Department of Veterinary Microbiology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu-614 625, Tamil Nadu, India.
Cite article:- Ponnusamy P., Reetha Lurthu T., Sasikala M., Ronald B.S.M., Selvaraj J., Puvarajan B., Manickam R. (2018). Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken. Indian Journal of Animal Research. 53(9): 1243-1245. doi: 10.18805/ijar.B-3640.

ABSTRACT

The present study was carried out to identify avian neoplastic viruses in the formalin fixed tissues of fancy chicken. The tissue samples collected during necropsy were examined by histopathology. It showed lymphoid and reticular cell infiltration in kidney, liver and lungs. For identification and differentiation of avian neoplastic viruses, PCR was performed using primer sets specific for Mareks disease virus, avian leukosis complex and reticuloendotheliosis virus. It was found that tumors were REV originated. Further confirmation, purified PCR product was subjected to sequencing. It showed 99% homology with other REV isolates available in the NCBI database. The present communication describes infection of a fancy chicken with REV on the basis of histopathological findings as well as molecular methods.

REFERENCES

  1. Buchen-Osmond, C. (2004). 00.061. Retroviridae. In: ICTVdB Management. ICTVdB - The Universal Virus Database, version 3. Columbia University, New York, USA.
  2. Crespo, R., Woolcock, P.R., Fadly, A.M., Hall, C., Shivaprasad,H. L. (2002). Characterization of T-cell lymphomas associated with an outbreak of reticuloendotheliosis in turkeys.Avian Pathology,31:355-361. 
  3. Davidson, I. and Silva, R. F. (2008). Creation of diversity in the animal virus world by inter-species and intra-species recombinations: lessons learned from poultry viruses. Virus Genes, 36: 1–10.
  4. García, M., Narrang, N., Reed, W.M., Fadly, A.M. (2003). Molecular characterization of reticuloendotheliosis virus insertions in the genome of field and vaccine strains of fowl poxvirus. Avian Diseases, 47:343–54 
  5. Kalyani, I.H., Joshi, C.G., Jhala, M.K., Bhanderi, B.B., Purohit, J.H. (2011). Characterization of 132 bp repeats BamH1-H region in pathogenic Marek’s disease virus of poultry in Gujarat, India, using PCR and sequencing. Indian Journal of Virology, 22(1): 72-75.
  6. Khordadmehr, M., Firouzamandi, M., Zehtab-Najafi, M., Shahbazi, R. (2017).Naturally occurring co-infection of avian Leukosis Virus (subgroups A-E) and reticuloendotheliosis virus in green Peafowls (Pavo muticus).Brazilian Journal of Poultry Science, 19(4):609-614.
  7. Nair, V. (2013). Neoplastic diseases. In: Diseases of poultry. Swayne DE, Glisson JR, McDougald LR, Nolan LK, Suarez DL, Nair N.13th edition. New York: John Wiley & Sons; p. 514.
  8. Nair, V., Zavala, G., and Fadly, A.M. (2013). Neoplastic diseases: Reticoloendotheliosis. In: Diseases of poultry. Swayne DE, Glisson JR, McDougald LR, Nolan LK, Suarez DL, Nair N.13th edition. New York: John Wiley & Sons; p.593-604.
  9. Prezotto, C.F., Marin, S.Y., Araujo, T.S., Barbosa, F.O., Barrios, P.R., Gomes, A.M., Peconick A.P., Resende, M., Sousa, R.V., Martins, N.R.S. (2016). Experimental coinfection of chicken anemia virus and Mycoplasma gallisepticum vaccine strains in broiler chicks. Brazilian Journal of Poultry Science, 18(3):475-480.
  10. Prukner-Radovèiæ, E., Lüshow, D., Ciglar Grozdaniæ, I., Tišljar, M., Mazija, H., Vranešic, Ð., Hafez, H.M. (2006). Isolation and molecular biological investigation of avian poxviruses from chickens, a turkey, and pigeon in Croatia. Avian Diseases,    50(3):440–444.
  11. Smith, L.M., Brown, S.R., Howes, K., McLeod, S., Arshad, S.S., Barron, G.S., et al. (1998). Development and application of polymerase chain reaction (PCR) tests for the detection of subgroup J avian leukosis virus. Virus Research,54:87-98.
  12. El-Sebelgy, M.M., Ahmed,B.M., Ata,N.S., Hussein,H.A. (2014). Molecular detection and characterization of reticuloendotheliosis virus in broiler breeder chickens with visceral tumors in Egypt.International Journal of Veterinary Science and Medicine,    2(1):21-26
  13. Wei, K., Sun, Z., Zhu, S., Guo, W., Sheng, P., Wang, Z., Zhao, C., Zhao, Q., Zhu, R.(2012). Probable congenital transmission of reticuloendotheliosis virus caused by vaccination with contaminated vaccines. PLoS One. 7:e43422.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 53 issue 9 (september 2019) : 1243-1245,   Doi: 10.18805/ijar.B-3640

    Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken

    P
    P. Ponnusamy
    T
    T. Lurthu Reetha
    M
    M. Sasikala
    B
    B.S.M. Ronald
    J
    J. Selvaraj
    B
    B. Puvarajan
    R
    R. Manickam
    1Department of Veterinary Microbiology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu-614 625, Tamil Nadu, India.
    Cite article:- Ponnusamy P., Reetha Lurthu T., Sasikala M., Ronald B.S.M., Selvaraj J., Puvarajan B., Manickam R. (2018). Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken. Indian Journal of Animal Research. 53(9): 1243-1245. doi: 10.18805/ijar.B-3640.

    ABSTRACT

    The present study was carried out to identify avian neoplastic viruses in the formalin fixed tissues of fancy chicken. The tissue samples collected during necropsy were examined by histopathology. It showed lymphoid and reticular cell infiltration in kidney, liver and lungs. For identification and differentiation of avian neoplastic viruses, PCR was performed using primer sets specific for Mareks disease virus, avian leukosis complex and reticuloendotheliosis virus. It was found that tumors were REV originated. Further confirmation, purified PCR product was subjected to sequencing. It showed 99% homology with other REV isolates available in the NCBI database. The present communication describes infection of a fancy chicken with REV on the basis of histopathological findings as well as molecular methods.

    REFERENCES

    1. Buchen-Osmond, C. (2004). 00.061. Retroviridae. In: ICTVdB Management. ICTVdB - The Universal Virus Database, version 3. Columbia University, New York, USA.
    2. Crespo, R., Woolcock, P.R., Fadly, A.M., Hall, C., Shivaprasad,H. L. (2002). Characterization of T-cell lymphomas associated with an outbreak of reticuloendotheliosis in turkeys.Avian Pathology,31:355-361. 
    3. Davidson, I. and Silva, R. F. (2008). Creation of diversity in the animal virus world by inter-species and intra-species recombinations: lessons learned from poultry viruses. Virus Genes, 36: 1–10.
    4. García, M., Narrang, N., Reed, W.M., Fadly, A.M. (2003). Molecular characterization of reticuloendotheliosis virus insertions in the genome of field and vaccine strains of fowl poxvirus. Avian Diseases, 47:343–54 
    5. Kalyani, I.H., Joshi, C.G., Jhala, M.K., Bhanderi, B.B., Purohit, J.H. (2011). Characterization of 132 bp repeats BamH1-H region in pathogenic Marek’s disease virus of poultry in Gujarat, India, using PCR and sequencing. Indian Journal of Virology, 22(1): 72-75.
    6. Khordadmehr, M., Firouzamandi, M., Zehtab-Najafi, M., Shahbazi, R. (2017).Naturally occurring co-infection of avian Leukosis Virus (subgroups A-E) and reticuloendotheliosis virus in green Peafowls (Pavo muticus).Brazilian Journal of Poultry Science, 19(4):609-614.
    7. Nair, V. (2013). Neoplastic diseases. In: Diseases of poultry. Swayne DE, Glisson JR, McDougald LR, Nolan LK, Suarez DL, Nair N.13th edition. New York: John Wiley & Sons; p. 514.
    8. Nair, V., Zavala, G., and Fadly, A.M. (2013). Neoplastic diseases: Reticoloendotheliosis. In: Diseases of poultry. Swayne DE, Glisson JR, McDougald LR, Nolan LK, Suarez DL, Nair N.13th edition. New York: John Wiley & Sons; p.593-604.
    9. Prezotto, C.F., Marin, S.Y., Araujo, T.S., Barbosa, F.O., Barrios, P.R., Gomes, A.M., Peconick A.P., Resende, M., Sousa, R.V., Martins, N.R.S. (2016). Experimental coinfection of chicken anemia virus and Mycoplasma gallisepticum vaccine strains in broiler chicks. Brazilian Journal of Poultry Science, 18(3):475-480.
    10. Prukner-Radovèiæ, E., Lüshow, D., Ciglar Grozdaniæ, I., Tišljar, M., Mazija, H., Vranešic, Ð., Hafez, H.M. (2006). Isolation and molecular biological investigation of avian poxviruses from chickens, a turkey, and pigeon in Croatia. Avian Diseases,    50(3):440–444.
    11. Smith, L.M., Brown, S.R., Howes, K., McLeod, S., Arshad, S.S., Barron, G.S., et al. (1998). Development and application of polymerase chain reaction (PCR) tests for the detection of subgroup J avian leukosis virus. Virus Research,54:87-98.
    12. El-Sebelgy, M.M., Ahmed,B.M., Ata,N.S., Hussein,H.A. (2014). Molecular detection and characterization of reticuloendotheliosis virus in broiler breeder chickens with visceral tumors in Egypt.International Journal of Veterinary Science and Medicine,    2(1):21-26
    13. Wei, K., Sun, Z., Zhu, S., Guo, W., Sheng, P., Wang, Z., Zhao, C., Zhao, Q., Zhu, R.(2012). Probable congenital transmission of reticuloendotheliosis virus caused by vaccination with contaminated vaccines. PLoS One. 7:e43422.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)