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Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Research Article

Indian Journal of Animal Research, volume 53 issue 9 (september 2019) : 1239-1242

Comparative pathogenicity analysis of Cryptococcus neoformans RAPD types

Aniruddha Banerjee, Saktipada Pradhan, Chanchal Debnath, Nimai Chand Patra, Rabindra Nath Hansda, Samiran Mondal, Rakesh Kumar, Abhishek Dharam Singh
1Department of Veterinary Pathology, West Bengal University of Animal and Fishery Sciences, Kolkata-700 037, West Bengal, India.
Cite article:- Banerjee Aniruddha, Pradhan Saktipada, Debnath Chanchal, Patra Chand Nimai, Hansda Nath Rabindra, Mondal Samiran, Kumar Rakesh, Singh Dharam Abhishek (2018). Comparative pathogenicity analysis of Cryptococcus neoformans RAPD types. Indian Journal of Animal Research. 53(9): 1239-1242. doi: 10.18805/ijar.B-3637.

ABSTRACT

Cryptococcus neoformans and Cryptococcus gattii are the major causes of Cryptococcosis, an invasive fungal infection of human and animals. Few data have been reported on the pathophysiology of Pattern I (PI) and Pattern IV (PIV) of C. neoformans with context of their clinical outcome. In this study an extensive comparison of PI and PIV was conducted to provide a more complete and reliable assessment of their biological and clinical behaviour. Representative strains of PI and PIV of C. neoformans were collected from Outreach Programme on Zoonotic Diseases (OPZD) at Department of Veterinary Public Health of West Bengal University of Animal and Fishery Sciences and infective inoculum was prepared by adjusting the number of Cryptococci at 3.25X 105 / ml in phosphate buffer saline (pH 7.4). Mice were challenged with 0.2 ml of inoculum at lateral tail vein and infected mice were examined for histopathological study and survivability study for 21 days. This present study showed that PIV produced more cystic structure in brain (c2 =4.22, P< 0.05) and granuloma in liver (c2=4.05, P<0.05) with lower mean survival time (P= 0.044) but lack a statistical significance in micro-abscess formation in kidney (c2=0.017, P> 0.05). PIV was associated with an approximate 4-fold increase in risk of death as compared with PI (P< 0.05, OR= 4.38, 95% CL= 1.32 to 14.50). Our study suggested that Pattern IV produced more severe Cryptococcosis in murine model due to its difference in genotype.

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