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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 9 (september 2019) : 1140-1143,   Doi: 10.18805/ijar.B-3634

Association of GnRH receptor gene polymorphism on testicular parameters and semen quality in HF crossbred bulls 

K
K.B. Iyer
M
M.V. Ingawale
S
S.V. Kuralkar
P
P.S. Bankar
S
S. Sajid Ali
R
R.S. Ingole
S
S.W. Hajare
C
C.H. Pawshe
1Department of Animal Reproduction, Gynaecology and Obstetrics, Post Graduate Institute of Veterinary & Animal Sciences, Akola-444 104, Maharashtra, India.
Cite article:- Iyer K.B., Ingawale M.V., Kuralkar S.V., Bankar P.S., Ali Sajid S., Ingole R.S., Hajare S.W., Pawshe C.H. (2018). Association of GnRH receptor gene polymorphism on testicular parameters and semen quality in HF crossbred bulls. Indian Journal of Animal Research. 53(9): 1140-1143. doi: 10.18805/ijar.B-3634.

ABSTRACT

The present research was conducted to study the association of GnRH receptor  gene polymorphism on testicular parameters and semen quality in fortyfour  HF crossbred bulls.  Two different genotypes GG and GA having amplification size 170, 118, 96 and 214, 170, 118 were identified, respectively. Genotypic frequencies were 0.82 (GG) and 0.18(GA) and gene frequency of G allele was 0.91 and for A allele was 0.09. The testicular parameters like scrotal circumference (SC), testicular width (TW), testicular thickness (TT) were non significant while testicular length (TL) was significant (P<0.05)   among two genotypes. Seminal attributes like semen volume (Vol) concentration (Conc.) initial motility (IM), post thaw motility (PTM), abnormal sperm rate (ASR) and acrosome integrity test (AIT)  were non significant while hypo osmotic swelling test (HOST) was significant (P<0.05)   among two genotypes. 

REFERENCES

  1. Bedecarrats G.Y., Linher, K.D., Janovick, J.A., Beranova, M., Kada, F., Seminara, S.B., Michael Conn, P.and Kaiser, U.B. (2003). Four naturally occurring mutations in the human GnRH receptor affect ligand binding and receptor function. Mol. Cell Endocrinol., 205:51–64.
  2. Cohen D.P. (2000). Molecular evaluation of the gonadotropinreleasing hormone receptor. Semin. Reprod. Med., 18:11–16.
  3. Dai L., Zhao, Z., Zhao, R., Xiao, S., Jiang, H., Yue, X., Li, X., Gao, Y., Liu, J. and Zhang, J. (2009). Effects of novel single nucleotide polymorphisms of the FSH beta-subunit gene on semen quality and fertility in bulls. Anim. Reprod. Sci., 114(1–3):14-22.
  4. Kaiser U.B., Jakubowiak, A., Steinberger, A. and Chin, W.W. (1993), Regulation of rat pituitary gonadotropin-releasing hormone receptor mRNA levels in vivo and in vitro. Endocrinology, 133(2):931–934.
  5. Lin C.L., Ponsuksili, S., Tholen, E., Jennen, D.G., Schellander, K. and Wimmers, K. (2006), Candidate gene markers for sperm quality and fertility of boar. Anim. Reprod. Sci., 92:349–363.
  6. Lirón J.P., Alberto, J., María Fernández, E., María Ripoli, V., Rogberg-Muñozm Andrés, Goszczynskim Daniel E, et al (2012). Association between GNRHR, LHR and IGF1 polymorphisms and timing of puberty in male Angus cattle. BMC Genet., 13: 26.
  7. Kumar Sushil, Ganguly, Indrajit., Rajib, Deb., Singh Umesh., Mandal, Dilip Kumar, et al. (2015). Elucidating the role of bovine GnRHR on semen quality traits among crossbred bull. Indian Journal of Animal Sciences, 85(12):1314-1317.
  8. Mathevon M., Buhr, M.M. and Dekkers, J.C. (1998). Environmental, management and genetic factors affecting semen production in Holstein bulls. J. Dairy Sci., 81:3321-3330.
  9. Millar R.P. (2005). GnRHs and GnRH receptors. Anim. Reprod. Sci., 88:5-28.
  10. Millar R.P. and Pawson, A.J. (2004). Outside in and inside out signalling: the new concept that selectivity of ligand binding at the gonadotropin-releasing hormone receptor is modulated by the intracellular environment. Endocrinology, 145:3590–3593.
  11. Ren D.R., Ren, J., Xing, Y.Y. and Guo, Y.M. (2009). A genome scan for quantitative trait loci affecting male reproductive traits in a White Duroc × Chinese Erhualian. Resource Population. J. Anim. Sci., 87:17-23.
  12. Schubert U., Anton, L.C., Gibbs, J., Norbury, C.C., Yewdell, J.W. and Bennink, J.R. (2002). Rapid degradation of a large fraction of newly synthesized proteins by proteasomes. Nature 404:770-774. 
  13. Simoni M., Gromoll, J. and Nieschlag, E. (1997). The follicle-stimulating hormone receptor: biochemistry, molecular biology and pathophysiology. Endo. Rev., 18:739-773.
  14. Stojilkovic S.S. and Catt, K. J. (1995). Expression and signal transduction pathways of gonadotropin-releasing hormone receptors. Recent Prog. Horm. Res., 50:161–205.
  15. Terao H., Ogawa, T., Yanagisawa, M., Miyake, M., Sano, F., Kita, K., Murakami, T. et al. (2009). A case of adult-onset idiopathic hypogonadotropic hypogonadism presenting with infertility. Hinyokika Kiyo., 55(7):437–439.
  16. Vincent L., Evans, G., Short, T.H., South Wood, O.I., Plastow, G.S., Tuggle, C.K. and Rothschild, M.F. (1998). The prolactin receptor gene is associated with increased litter size in pigs. In: Proceedings of the 6th World Congress on Genet. Appl. Live. Prod., 27:15-18.
  17. Volpi R., Ugolotti, M.C., Carbucicchio, A. and Delsignore, R. (2008). Idiopathic hypogonadotropic hypogonadism in selective human gonadotropin deficiency in adult man: a case report. Acta. Biomed., 79(3):251–254.
  18. Weinbauer G.F. and Nieschlag, E. (1995). Gonadotrophin control of testicular germ cell development. Adv. Exp. Med. Biol., 377:55–65.
  19. Xiao-peng A.N., Dan, H., Jin-Xin, H., Guang, L., Ya-Na, W. and Ling, L. (2010). Polymorphism of exon 2 of FSH â gene and its relationship with reproduction performance in two goat breeds. Agri. Sci., 6:889-886.
  20. Xinyan L., Zhihua, J., Lingling, W., Yan, Z., Jinming, H. and Chao, Q. (2011). Effects of MboII and BspMI polymorphisms in the gonadotropin releasing hormone receptor (GnRHR) gene on sperm quality in Holstein bulls. Mol . Biol. Rep., 38:3411-3415.
  21. Yang W.C., Tang, K.Q., Yu, J.N., Zhang, C.Y., Zhang, X.X. and Yang, L.G. (2011). Effects of MboII and BspMI polymorphisms in the gonadotropin releasing hormone receptor (GnRHR) gene on sperm quality on sperm quality in Holstein bulls. Mol. Biol. Rep., 38:3411–3415.
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    Research Article

    volume 53 issue 9 (september 2019) : 1140-1143,   Doi: 10.18805/ijar.B-3634

    Association of GnRH receptor gene polymorphism on testicular parameters and semen quality in HF crossbred bulls 

    K
    K.B. Iyer
    M
    M.V. Ingawale
    S
    S.V. Kuralkar
    P
    P.S. Bankar
    S
    S. Sajid Ali
    R
    R.S. Ingole
    S
    S.W. Hajare
    C
    C.H. Pawshe
    1Department of Animal Reproduction, Gynaecology and Obstetrics, Post Graduate Institute of Veterinary & Animal Sciences, Akola-444 104, Maharashtra, India.
    Cite article:- Iyer K.B., Ingawale M.V., Kuralkar S.V., Bankar P.S., Ali Sajid S., Ingole R.S., Hajare S.W., Pawshe C.H. (2018). Association of GnRH receptor gene polymorphism on testicular parameters and semen quality in HF crossbred bulls. Indian Journal of Animal Research. 53(9): 1140-1143. doi: 10.18805/ijar.B-3634.

    ABSTRACT

    The present research was conducted to study the association of GnRH receptor  gene polymorphism on testicular parameters and semen quality in fortyfour  HF crossbred bulls.  Two different genotypes GG and GA having amplification size 170, 118, 96 and 214, 170, 118 were identified, respectively. Genotypic frequencies were 0.82 (GG) and 0.18(GA) and gene frequency of G allele was 0.91 and for A allele was 0.09. The testicular parameters like scrotal circumference (SC), testicular width (TW), testicular thickness (TT) were non significant while testicular length (TL) was significant (P<0.05)   among two genotypes. Seminal attributes like semen volume (Vol) concentration (Conc.) initial motility (IM), post thaw motility (PTM), abnormal sperm rate (ASR) and acrosome integrity test (AIT)  were non significant while hypo osmotic swelling test (HOST) was significant (P<0.05)   among two genotypes. 

    REFERENCES

    1. Bedecarrats G.Y., Linher, K.D., Janovick, J.A., Beranova, M., Kada, F., Seminara, S.B., Michael Conn, P.and Kaiser, U.B. (2003). Four naturally occurring mutations in the human GnRH receptor affect ligand binding and receptor function. Mol. Cell Endocrinol., 205:51–64.
    2. Cohen D.P. (2000). Molecular evaluation of the gonadotropinreleasing hormone receptor. Semin. Reprod. Med., 18:11–16.
    3. Dai L., Zhao, Z., Zhao, R., Xiao, S., Jiang, H., Yue, X., Li, X., Gao, Y., Liu, J. and Zhang, J. (2009). Effects of novel single nucleotide polymorphisms of the FSH beta-subunit gene on semen quality and fertility in bulls. Anim. Reprod. Sci., 114(1–3):14-22.
    4. Kaiser U.B., Jakubowiak, A., Steinberger, A. and Chin, W.W. (1993), Regulation of rat pituitary gonadotropin-releasing hormone receptor mRNA levels in vivo and in vitro. Endocrinology, 133(2):931–934.
    5. Lin C.L., Ponsuksili, S., Tholen, E., Jennen, D.G., Schellander, K. and Wimmers, K. (2006), Candidate gene markers for sperm quality and fertility of boar. Anim. Reprod. Sci., 92:349–363.
    6. Lirón J.P., Alberto, J., María Fernández, E., María Ripoli, V., Rogberg-Muñozm Andrés, Goszczynskim Daniel E, et al (2012). Association between GNRHR, LHR and IGF1 polymorphisms and timing of puberty in male Angus cattle. BMC Genet., 13: 26.
    7. Kumar Sushil, Ganguly, Indrajit., Rajib, Deb., Singh Umesh., Mandal, Dilip Kumar, et al. (2015). Elucidating the role of bovine GnRHR on semen quality traits among crossbred bull. Indian Journal of Animal Sciences, 85(12):1314-1317.
    8. Mathevon M., Buhr, M.M. and Dekkers, J.C. (1998). Environmental, management and genetic factors affecting semen production in Holstein bulls. J. Dairy Sci., 81:3321-3330.
    9. Millar R.P. (2005). GnRHs and GnRH receptors. Anim. Reprod. Sci., 88:5-28.
    10. Millar R.P. and Pawson, A.J. (2004). Outside in and inside out signalling: the new concept that selectivity of ligand binding at the gonadotropin-releasing hormone receptor is modulated by the intracellular environment. Endocrinology, 145:3590–3593.
    11. Ren D.R., Ren, J., Xing, Y.Y. and Guo, Y.M. (2009). A genome scan for quantitative trait loci affecting male reproductive traits in a White Duroc × Chinese Erhualian. Resource Population. J. Anim. Sci., 87:17-23.
    12. Schubert U., Anton, L.C., Gibbs, J., Norbury, C.C., Yewdell, J.W. and Bennink, J.R. (2002). Rapid degradation of a large fraction of newly synthesized proteins by proteasomes. Nature 404:770-774. 
    13. Simoni M., Gromoll, J. and Nieschlag, E. (1997). The follicle-stimulating hormone receptor: biochemistry, molecular biology and pathophysiology. Endo. Rev., 18:739-773.
    14. Stojilkovic S.S. and Catt, K. J. (1995). Expression and signal transduction pathways of gonadotropin-releasing hormone receptors. Recent Prog. Horm. Res., 50:161–205.
    15. Terao H., Ogawa, T., Yanagisawa, M., Miyake, M., Sano, F., Kita, K., Murakami, T. et al. (2009). A case of adult-onset idiopathic hypogonadotropic hypogonadism presenting with infertility. Hinyokika Kiyo., 55(7):437–439.
    16. Vincent L., Evans, G., Short, T.H., South Wood, O.I., Plastow, G.S., Tuggle, C.K. and Rothschild, M.F. (1998). The prolactin receptor gene is associated with increased litter size in pigs. In: Proceedings of the 6th World Congress on Genet. Appl. Live. Prod., 27:15-18.
    17. Volpi R., Ugolotti, M.C., Carbucicchio, A. and Delsignore, R. (2008). Idiopathic hypogonadotropic hypogonadism in selective human gonadotropin deficiency in adult man: a case report. Acta. Biomed., 79(3):251–254.
    18. Weinbauer G.F. and Nieschlag, E. (1995). Gonadotrophin control of testicular germ cell development. Adv. Exp. Med. Biol., 377:55–65.
    19. Xiao-peng A.N., Dan, H., Jin-Xin, H., Guang, L., Ya-Na, W. and Ling, L. (2010). Polymorphism of exon 2 of FSH â gene and its relationship with reproduction performance in two goat breeds. Agri. Sci., 6:889-886.
    20. Xinyan L., Zhihua, J., Lingling, W., Yan, Z., Jinming, H. and Chao, Q. (2011). Effects of MboII and BspMI polymorphisms in the gonadotropin releasing hormone receptor (GnRHR) gene on sperm quality in Holstein bulls. Mol . Biol. Rep., 38:3411-3415.
    21. Yang W.C., Tang, K.Q., Yu, J.N., Zhang, C.Y., Zhang, X.X. and Yang, L.G. (2011). Effects of MboII and BspMI polymorphisms in the gonadotropin releasing hormone receptor (GnRHR) gene on sperm quality on sperm quality in Holstein bulls. Mol. Biol. Rep., 38:3411–3415.
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