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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 8 (august 2019) : 991-996,   Doi: 10.18805/ijar.B-3620

Genetic diversity analysis of C-type lectin like receptors genes in indigenous and white leghorn chickens

S
Santosh Haunshi
A
Arun Kumar Burramsetty
K
Kannaki T. Ramasamy
1ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad-500 030, Telangana, India.
Cite article:- Haunshi Santosh, Burramsetty Kumar Arun, Ramasamy T. Kannaki (2018). Genetic diversity analysis of C-type lectin like receptors genes in indigenous and white leghorn chickens. Indian Journal of Animal Research. 53(8): 991-996. doi: 10.18805/ijar.B-3620.

ABSTRACT

C-type lectin like receptors (CLRs), play an important role in immune functions of the organisms. In chicken, B-NK and B-Lec genes encode CLRs that contain C-type lectin like domain. Polymorphism in CDS of B-NK and B-Lec genes were investigated in Ghagus, Nicobari and WLH breeds. Significant variations in polymorphisms were observed among three breeds. Highest number of SNPs was observed in Ghagus followed by Nicobari and WLH breed. Among two genes higher number of SNPs was detected in B-NK as compared to B-Lec gene. In B-NK gene, a total of 32 SNPs were detected in three breeds (24 in Ghagus, 23 in Nicobari and 17 in WLH). The interesting feature of polymorphism in this gene was that almost all SNPs were of NS type. A six bp identical insertion resulting in addition of two amino acids with open reading frame of 681bp was observed in Nicobari and WLH and no such insertion was observed in Ghagus. In B-lec gene, a total of 8 SNPs were detected in Ghagus while only 2 SNPs each in Nicobari and WLH breeds were detected. Phylogenetic analysis of coding sequence nucleotides of B-NK gene clustered Ghagus and red jungle fowl into a separate cluster. Study revealed higher variability in coding sequence of CLR genes in the form of higher SNPs in indigenous breeds of chicken.

REFERENCES

  1. Besbes, B. (2009). Genotype evaluation and breeding of poultry for performance under sub-optimal village conditions. World’s Poultry Science Journal.65:260-271.
  2. Blaha, J., Kalouskova, B., Skorepa, O., Pazický, S., Novak, P., Vanek, V. (2017). High-level expression and purification of soluble form of human natural killer cell receptor NKR-P1 in HEK293S GnTI- cells. Protein Expression and Purification.140: 36-43.
  3. Boonyanuwat, K., Thummabutra, S., Sookmanee, N., Vatchavalkhu, V., Siripholvat, V. (2006). Influences of major histocompatibility complex class I haplotypes on avian influenza virus disease traits in Thai indigenous chickens. Animal Science Journal.77:285–    289.
  4. Dambuza, I.M. and Brown, G.D. (2015). C-type lectins in immunity: recent developments. Current Opinion in Immunology.32:21–27.
  5. Hoving, J.C., Wilson, G.J., Brown, G.D. (2014). Signalling C-Type lectin receptors, microbial recognition and immunity. Cell Microbiology.16(2):185–194.
  6. Kumar, S., Stecher, G., Tamura, K. (2017). MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets MEGA7: Molecular Biology and Evolution.33(7):1870–1874 doi:10.1093/molbev/msw054.
  7. Letunic I, Doerks T, Bork P. (2012). SMART 7. recent updates to the protein domain annotation resource. Nucleic Acids Research.40 (D1): D302-D305.
  8. Matsuu, A., Kobayashi, T., Patchimasiri, T., Shiina, T, Suzuki, S., Chaichoune, K., Ratanakorn, P., Hiromoto, Y., Abe, H., Parchariyanon, S., Saito, T. (2016). Pathogenicity of genetically similar, H5N1 highly pathogenic avian influenza virus strains in chicken and the differences in sensitivity among different chicken breeds. PLOS One. 11(4). e0153649. doi:10.1371/journal.pone.    0153649.
  9. Netea, M.G., van der Meer, J.W.M. (2011). Immunodeficiency and genetic defects of pattern recognition receptors. New England Journal of Medicine.364:60-70.
  10. Psifidi A, Banos G, Matika S, Desta, T.T., Bettridge, J., Hume, D.A., Dessie, T., Christley, R., Wigley, P., Hanotte, O., Kaiser, P. (2016). Genome wide association studies of immune, disease and production traits in indigenous chicken ecotypes.Genet Selection Evolution.    48:74 (1-16).
  11. Rogers, S.L. and Kaufman, J. (2008). High allelic polymorphism, moderate sequence diversity and diversifying selection for B-NK but not B-lec, the pair of lectin-like receptor genes in the chicken MHC. Immunogenetics. 60(8):461-75.
  12. Rogers, S.L., Göbel, T.W., Viertlboeck, B.C., Milne, S., Beck, S., Kaufman, J. (2005). Characterization of the chicken C-type lectin-    like receptors B-NK and B-lec suggests that the NK complex and the MHC share a common ancestral region. Journal of Immunology.174:3475-3483.
  13. Saitou, N. and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 4(4):406–425.
  14. Sattler, S., Ghadially, H., Hofer, E. (2012). Evolution of the C-type lectin-like receptor genes of the DECTIN-1 cluster in the NK gene complex B-NK. The Scientific World Journal. Volume 2012, Article ID 931386, 11 pages doi:10.1100/2012/931386.
  15. Viertlboeck, B.C., Wortmann, A., Schmitt, R., Plachy, J., Gobel, T.W. (2008). Chicken C-type lectin-like receptor B-NK, expressed on NK and T cell subsets, binds to a ligand on activated splenocytes. Molecular Immunology.45:1398–1404. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 8 (august 2019) : 991-996,   Doi: 10.18805/ijar.B-3620

    Genetic diversity analysis of C-type lectin like receptors genes in indigenous and white leghorn chickens

    S
    Santosh Haunshi
    A
    Arun Kumar Burramsetty
    K
    Kannaki T. Ramasamy
    1ICAR-Directorate of Poultry Research, Rajendranagar, Hyderabad-500 030, Telangana, India.
    Cite article:- Haunshi Santosh, Burramsetty Kumar Arun, Ramasamy T. Kannaki (2018). Genetic diversity analysis of C-type lectin like receptors genes in indigenous and white leghorn chickens. Indian Journal of Animal Research. 53(8): 991-996. doi: 10.18805/ijar.B-3620.

    ABSTRACT

    C-type lectin like receptors (CLRs), play an important role in immune functions of the organisms. In chicken, B-NK and B-Lec genes encode CLRs that contain C-type lectin like domain. Polymorphism in CDS of B-NK and B-Lec genes were investigated in Ghagus, Nicobari and WLH breeds. Significant variations in polymorphisms were observed among three breeds. Highest number of SNPs was observed in Ghagus followed by Nicobari and WLH breed. Among two genes higher number of SNPs was detected in B-NK as compared to B-Lec gene. In B-NK gene, a total of 32 SNPs were detected in three breeds (24 in Ghagus, 23 in Nicobari and 17 in WLH). The interesting feature of polymorphism in this gene was that almost all SNPs were of NS type. A six bp identical insertion resulting in addition of two amino acids with open reading frame of 681bp was observed in Nicobari and WLH and no such insertion was observed in Ghagus. In B-lec gene, a total of 8 SNPs were detected in Ghagus while only 2 SNPs each in Nicobari and WLH breeds were detected. Phylogenetic analysis of coding sequence nucleotides of B-NK gene clustered Ghagus and red jungle fowl into a separate cluster. Study revealed higher variability in coding sequence of CLR genes in the form of higher SNPs in indigenous breeds of chicken.

    REFERENCES

    1. Besbes, B. (2009). Genotype evaluation and breeding of poultry for performance under sub-optimal village conditions. World’s Poultry Science Journal.65:260-271.
    2. Blaha, J., Kalouskova, B., Skorepa, O., Pazický, S., Novak, P., Vanek, V. (2017). High-level expression and purification of soluble form of human natural killer cell receptor NKR-P1 in HEK293S GnTI- cells. Protein Expression and Purification.140: 36-43.
    3. Boonyanuwat, K., Thummabutra, S., Sookmanee, N., Vatchavalkhu, V., Siripholvat, V. (2006). Influences of major histocompatibility complex class I haplotypes on avian influenza virus disease traits in Thai indigenous chickens. Animal Science Journal.77:285–    289.
    4. Dambuza, I.M. and Brown, G.D. (2015). C-type lectins in immunity: recent developments. Current Opinion in Immunology.32:21–27.
    5. Hoving, J.C., Wilson, G.J., Brown, G.D. (2014). Signalling C-Type lectin receptors, microbial recognition and immunity. Cell Microbiology.16(2):185–194.
    6. Kumar, S., Stecher, G., Tamura, K. (2017). MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets MEGA7: Molecular Biology and Evolution.33(7):1870–1874 doi:10.1093/molbev/msw054.
    7. Letunic I, Doerks T, Bork P. (2012). SMART 7. recent updates to the protein domain annotation resource. Nucleic Acids Research.40 (D1): D302-D305.
    8. Matsuu, A., Kobayashi, T., Patchimasiri, T., Shiina, T, Suzuki, S., Chaichoune, K., Ratanakorn, P., Hiromoto, Y., Abe, H., Parchariyanon, S., Saito, T. (2016). Pathogenicity of genetically similar, H5N1 highly pathogenic avian influenza virus strains in chicken and the differences in sensitivity among different chicken breeds. PLOS One. 11(4). e0153649. doi:10.1371/journal.pone.    0153649.
    9. Netea, M.G., van der Meer, J.W.M. (2011). Immunodeficiency and genetic defects of pattern recognition receptors. New England Journal of Medicine.364:60-70.
    10. Psifidi A, Banos G, Matika S, Desta, T.T., Bettridge, J., Hume, D.A., Dessie, T., Christley, R., Wigley, P., Hanotte, O., Kaiser, P. (2016). Genome wide association studies of immune, disease and production traits in indigenous chicken ecotypes.Genet Selection Evolution.    48:74 (1-16).
    11. Rogers, S.L. and Kaufman, J. (2008). High allelic polymorphism, moderate sequence diversity and diversifying selection for B-NK but not B-lec, the pair of lectin-like receptor genes in the chicken MHC. Immunogenetics. 60(8):461-75.
    12. Rogers, S.L., Göbel, T.W., Viertlboeck, B.C., Milne, S., Beck, S., Kaufman, J. (2005). Characterization of the chicken C-type lectin-    like receptors B-NK and B-lec suggests that the NK complex and the MHC share a common ancestral region. Journal of Immunology.174:3475-3483.
    13. Saitou, N. and Nei, M. (1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution. 4(4):406–425.
    14. Sattler, S., Ghadially, H., Hofer, E. (2012). Evolution of the C-type lectin-like receptor genes of the DECTIN-1 cluster in the NK gene complex B-NK. The Scientific World Journal. Volume 2012, Article ID 931386, 11 pages doi:10.1100/2012/931386.
    15. Viertlboeck, B.C., Wortmann, A., Schmitt, R., Plachy, J., Gobel, T.W. (2008). Chicken C-type lectin-like receptor B-NK, expressed on NK and T cell subsets, binds to a ligand on activated splenocytes. Molecular Immunology.45:1398–1404. 
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