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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 8 (august 2019) : 1014-1019,   Doi: 10.18805/ijar.B-3613

Effect of imidacloprid on antioxidant status and histopathological changes in ovary and uterus of adult female wistar rats
 

A
Archana Lohiya
V
Vinod Kumar
J
J.S. Punia
1Department of Veterinary Pharmacology and Toxicology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 001, India.
Cite article:- Lohiya Archana, Kumar Vinod, Punia J.S. (2018). Effect of imidacloprid on antioxidant status and histopathological changes in ovary and uterus of adult female wistar rats. Indian Journal of Animal Research. 53(8): 1014-1019. doi: 10.18805/ijar.B-3613.

ABSTRACT

Imidacloprid is a neonicotinoid insecticide and has been extensively used as a crop pest and in pet flea control programme. In the present study, the effects of imidacloprid on ovary and uteri tissue was analyzed in adult female Wistar rats at two dose levels (19 and 38 mg/kg/day) administered orally for 10, 20 and 30 days. Effects were compared with respective control animals administered daily with 2% gum acacia. Parameters undertaken were organ weight, levels of cytoplasmic and membrane proteins, oxidative stress parameters viz. activities of SOD, GPx and levels of GSH and MDA and histopathological changes. IMI (38 mg/kg, 30 days) reduced cytoplamic proteins in both ovaries and uteri whereas this dose reduced membrane protein in ovaries only. IMI (38 mg/kg, 20 and 30 days) decreased SOD enzyme in both ovaries and uteri and GSH-Px levels in ovaries only. The GSH-Px levels were also seen with decreased levels in uteri with IMI (38 mg/kg) for 30 days. IMI (38 mg/kg, 20 and 30 days) induces degenerative changes in ovaries of rats. Hence, it is concluded from the present studies that administration of higher doses (38 mg/kg/day) of IMI for 20 and 30 days generated oxidative stress in ovaries and uteri of female rats. 

REFERENCES

  1. Agarwal A., Gupta S., R.K. Sharma R.K. (2005) Role of oxidative stress in female reproduction. Reprod. Biol. Endocrinol3: 28–41.
  2. Aly, H., Domenech, O., Ashraf, B., Abdel, N., (2009) Aroclor 1254 impairs spermatogenesis and induces oxidative stress in rat testicular mitochondria. Food Chem. Toxicol 47 (8): 1733–1738.
  3. Berny P.J., Florence B., Bernadette V., Videmann T.B. (1999) Evaluation of the toxicity of imidacloprid in wild birds. A new high performance thin layer chromatography method for the analysis of liver and crop samples in suspected poisoning cases. J. Liq. Chromatogr. Related Technol 22: 1547–1559.
  4. Beutler E., Duron O., Kelly B.M., (1963). Improved Method for the Determination of Blood Glutathione. Journal of Laboratory and Clinical Medicine 61: 882–888.
  5. Booth NH. Inhalant anesthetics. In: [Booth NH, McDonald LE, (eds)]. Veterinary pharmacology and therapeutics. 6th ed. Ames, Iowa: Iowa State Univeristy Press, 1988: 181–211.
  6. Debnath D., Mandal T.K. (2000). Study of Quinalphos (an environmental oestrogeninsecticide) formulation (ekalux 25E.C.)- induced damage of the testicular tissues and antioxidant defense systems in Sprague–Dawley Albino rats, J.Appl. Toxicol. 20 197–204.
  7. Grosicka-Macia G.E., Dagmara K.P., Maria S., Tomasz G., Iwonna R.S., (2011) Protective effect of N-acetyl-l-cysteine against maneb induced oxidative and apoptotic injury in Chinese hamster V79 cells. Food Chem. Toxicol49 (4): 1020–1025.
  8. Guney M., Baha O., Hilmi D., Gulnur T., Seren G.G., Irfan A., Tamer M., (2007a) Fallopian damage induced by organophosphate insecticide methyl parathion and protective effect of vitamins E and C on ultrastructural changes in rats.Toxicol. Ind. Health 23: 429–438.
  9. Güney M., Hilmi D., Baha O., Meltem O., Gokhan B., Irfan A., (2007b) Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C Hum. Exp. Toxicol., 26: 491–498.
  10. Hafeman D.G., Sunde R.A., Hoeskstra W.G. (1974). Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J. Nutr.104: 580-587
  11. Harper A.J., Rodwell V.M., Meyer P.A. (1977). Review of Physiological Chemistry,16th ed.
  12. Khan M.R., Dawood A., (2009) Protective effects of Digera muricata (L.) Mart. on testis against oxidative stress of carbon tetrachloride in rat.Food Chem. Toxicol. 47 (6): 1393–1399.
  13. Luna L.G. (1968). Manual of Histological Staining Method of Arms Forces. Institute of pathology, 3 ed. Mc Graw Hills Book Company.
  14. Madesh M., Balsubramanian K.A. (1998). Microtitre plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J. Biochemistry and Biophysics 35: pp 184-188.
  15. Matsuda K., Buckingham S.D., Klier D., Rauh J.J., Marta G., Sattelle D.B.(2001). Neonicotinoides: insecticides acting on insect nicotinic acetylcholine receptors, Trends Pharmacol. Sci.22: 573–580.
  16. Mencke N., Jeschke P. (2002) Therapy and Prevention of Parasitic Insects in Veterinary Medicine using Imidacloprid. Curr Top Med Chem. 2: 701–15.
  17. NPIC (National Pesticide Information Centre). 2010. “Imidacloprid Technical Fact Sheet”. Available at: http://npic.orst.edu/factsheets/    imidacloprid.pdf
  18. Ohkawa H., Ohishi N., Yagi K. (1972). Assay for Lipid Peroxides in Female rats Tissues by Thiobarbituric Acid Reaction. Analytical Biochemistry 95: 351–358.
  19. Panse V.G., Sukhatme D.V. (1978). Statistical Method for Agricultural Workers, 3rd ed., ICAR, New Delhi.
  20. Proenca P., Teixeira H., Castanheira F., Pinheiro J., Monsanto P.V. (2005) Two fatal intoxication cases with imidacloprid.LC/MS analysis Forensic Sci. Int. 153: 75–80.
  21. Richardson R. J. (1981). Toxicology of the nervous system. Toxicology Principles and Practice, [A. L. Reeves (Ed)], Wiley Inter Science Publishers, Toranto
  22. Sagsoz N., Kisa U., Apan A. (2002) Ischaemia-reperfusion injury of rat ovary and effectsof vitamin C, mannitol and verapamil Hum. Reprod. 17: 2972–2976.
  23. Singh M., Sandhir R., Kiran R. (2006) Erythrocyte antioxidant enzymes in toxicological evaluation of commonly used organophosphate pesticides. Indian J. Exp. Biol.44: 580–583.
  24. Tomizawa M., Casida J.E. (2003) Selective toxicity of neonicotinoids attributable to specificity of insect and mammalian nicotinic receptors. Ann Rev Entomol. 48: 339–64.
  25. Tomlin, C. D. S (2006). The Pesticide Manual, A World Compendium, 14th ed.; British Crop Protection Council: Surry, England,; pp 598-599.
  26. Uzun F.G., Kalender S., Durak D., Demir D., Kalender Y. (2009) Malathion-induced testicular toxicity in male rats and the protective effect of vitamins C and E. Food Chem. Toxicol. 47 (8) (2009): 1903–1908
  27. Wu W., Ja L., Tsung C.E. (2001) Acute poisoning with nicotinoid insecticide imidacloprid in N-methyl pyrrlidone. Clin. Toxicol. 39 (6): 617–621.
  28. Zama D., Meraihi Z., Tebibel S., Benayssa W., Benayache F., Benayache S., Vlitinck A.J. (2007) Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: the protective role of the butanolic extract of Paronychie argentea L.Indian Pharmacol. 39: 145–150 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 8 (august 2019) : 1014-1019,   Doi: 10.18805/ijar.B-3613

    Effect of imidacloprid on antioxidant status and histopathological changes in ovary and uterus of adult female wistar rats
     

    A
    Archana Lohiya
    V
    Vinod Kumar
    J
    J.S. Punia
    1Department of Veterinary Pharmacology and Toxicology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125 001, India.
    Cite article:- Lohiya Archana, Kumar Vinod, Punia J.S. (2018). Effect of imidacloprid on antioxidant status and histopathological changes in ovary and uterus of adult female wistar rats. Indian Journal of Animal Research. 53(8): 1014-1019. doi: 10.18805/ijar.B-3613.

    ABSTRACT

    Imidacloprid is a neonicotinoid insecticide and has been extensively used as a crop pest and in pet flea control programme. In the present study, the effects of imidacloprid on ovary and uteri tissue was analyzed in adult female Wistar rats at two dose levels (19 and 38 mg/kg/day) administered orally for 10, 20 and 30 days. Effects were compared with respective control animals administered daily with 2% gum acacia. Parameters undertaken were organ weight, levels of cytoplasmic and membrane proteins, oxidative stress parameters viz. activities of SOD, GPx and levels of GSH and MDA and histopathological changes. IMI (38 mg/kg, 30 days) reduced cytoplamic proteins in both ovaries and uteri whereas this dose reduced membrane protein in ovaries only. IMI (38 mg/kg, 20 and 30 days) decreased SOD enzyme in both ovaries and uteri and GSH-Px levels in ovaries only. The GSH-Px levels were also seen with decreased levels in uteri with IMI (38 mg/kg) for 30 days. IMI (38 mg/kg, 20 and 30 days) induces degenerative changes in ovaries of rats. Hence, it is concluded from the present studies that administration of higher doses (38 mg/kg/day) of IMI for 20 and 30 days generated oxidative stress in ovaries and uteri of female rats. 

    REFERENCES

    1. Agarwal A., Gupta S., R.K. Sharma R.K. (2005) Role of oxidative stress in female reproduction. Reprod. Biol. Endocrinol3: 28–41.
    2. Aly, H., Domenech, O., Ashraf, B., Abdel, N., (2009) Aroclor 1254 impairs spermatogenesis and induces oxidative stress in rat testicular mitochondria. Food Chem. Toxicol 47 (8): 1733–1738.
    3. Berny P.J., Florence B., Bernadette V., Videmann T.B. (1999) Evaluation of the toxicity of imidacloprid in wild birds. A new high performance thin layer chromatography method for the analysis of liver and crop samples in suspected poisoning cases. J. Liq. Chromatogr. Related Technol 22: 1547–1559.
    4. Beutler E., Duron O., Kelly B.M., (1963). Improved Method for the Determination of Blood Glutathione. Journal of Laboratory and Clinical Medicine 61: 882–888.
    5. Booth NH. Inhalant anesthetics. In: [Booth NH, McDonald LE, (eds)]. Veterinary pharmacology and therapeutics. 6th ed. Ames, Iowa: Iowa State Univeristy Press, 1988: 181–211.
    6. Debnath D., Mandal T.K. (2000). Study of Quinalphos (an environmental oestrogeninsecticide) formulation (ekalux 25E.C.)- induced damage of the testicular tissues and antioxidant defense systems in Sprague–Dawley Albino rats, J.Appl. Toxicol. 20 197–204.
    7. Grosicka-Macia G.E., Dagmara K.P., Maria S., Tomasz G., Iwonna R.S., (2011) Protective effect of N-acetyl-l-cysteine against maneb induced oxidative and apoptotic injury in Chinese hamster V79 cells. Food Chem. Toxicol49 (4): 1020–1025.
    8. Guney M., Baha O., Hilmi D., Gulnur T., Seren G.G., Irfan A., Tamer M., (2007a) Fallopian damage induced by organophosphate insecticide methyl parathion and protective effect of vitamins E and C on ultrastructural changes in rats.Toxicol. Ind. Health 23: 429–438.
    9. Güney M., Hilmi D., Baha O., Meltem O., Gokhan B., Irfan A., (2007b) Ovarian toxicity in rats caused by methidathion and ameliorating effect of vitamins E and C Hum. Exp. Toxicol., 26: 491–498.
    10. Hafeman D.G., Sunde R.A., Hoeskstra W.G. (1974). Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rat. J. Nutr.104: 580-587
    11. Harper A.J., Rodwell V.M., Meyer P.A. (1977). Review of Physiological Chemistry,16th ed.
    12. Khan M.R., Dawood A., (2009) Protective effects of Digera muricata (L.) Mart. on testis against oxidative stress of carbon tetrachloride in rat.Food Chem. Toxicol. 47 (6): 1393–1399.
    13. Luna L.G. (1968). Manual of Histological Staining Method of Arms Forces. Institute of pathology, 3 ed. Mc Graw Hills Book Company.
    14. Madesh M., Balsubramanian K.A. (1998). Microtitre plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J. Biochemistry and Biophysics 35: pp 184-188.
    15. Matsuda K., Buckingham S.D., Klier D., Rauh J.J., Marta G., Sattelle D.B.(2001). Neonicotinoides: insecticides acting on insect nicotinic acetylcholine receptors, Trends Pharmacol. Sci.22: 573–580.
    16. Mencke N., Jeschke P. (2002) Therapy and Prevention of Parasitic Insects in Veterinary Medicine using Imidacloprid. Curr Top Med Chem. 2: 701–15.
    17. NPIC (National Pesticide Information Centre). 2010. “Imidacloprid Technical Fact Sheet”. Available at: http://npic.orst.edu/factsheets/    imidacloprid.pdf
    18. Ohkawa H., Ohishi N., Yagi K. (1972). Assay for Lipid Peroxides in Female rats Tissues by Thiobarbituric Acid Reaction. Analytical Biochemistry 95: 351–358.
    19. Panse V.G., Sukhatme D.V. (1978). Statistical Method for Agricultural Workers, 3rd ed., ICAR, New Delhi.
    20. Proenca P., Teixeira H., Castanheira F., Pinheiro J., Monsanto P.V. (2005) Two fatal intoxication cases with imidacloprid.LC/MS analysis Forensic Sci. Int. 153: 75–80.
    21. Richardson R. J. (1981). Toxicology of the nervous system. Toxicology Principles and Practice, [A. L. Reeves (Ed)], Wiley Inter Science Publishers, Toranto
    22. Sagsoz N., Kisa U., Apan A. (2002) Ischaemia-reperfusion injury of rat ovary and effectsof vitamin C, mannitol and verapamil Hum. Reprod. 17: 2972–2976.
    23. Singh M., Sandhir R., Kiran R. (2006) Erythrocyte antioxidant enzymes in toxicological evaluation of commonly used organophosphate pesticides. Indian J. Exp. Biol.44: 580–583.
    24. Tomizawa M., Casida J.E. (2003) Selective toxicity of neonicotinoids attributable to specificity of insect and mammalian nicotinic receptors. Ann Rev Entomol. 48: 339–64.
    25. Tomlin, C. D. S (2006). The Pesticide Manual, A World Compendium, 14th ed.; British Crop Protection Council: Surry, England,; pp 598-599.
    26. Uzun F.G., Kalender S., Durak D., Demir D., Kalender Y. (2009) Malathion-induced testicular toxicity in male rats and the protective effect of vitamins C and E. Food Chem. Toxicol. 47 (8) (2009): 1903–1908
    27. Wu W., Ja L., Tsung C.E. (2001) Acute poisoning with nicotinoid insecticide imidacloprid in N-methyl pyrrlidone. Clin. Toxicol. 39 (6): 617–621.
    28. Zama D., Meraihi Z., Tebibel S., Benayssa W., Benayache F., Benayache S., Vlitinck A.J. (2007) Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: the protective role of the butanolic extract of Paronychie argentea L.Indian Pharmacol. 39: 145–150 
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