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Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Research Article

Indian Journal of Animal Research, volume 53 issue 7 (july 2019) : 959-962

Detection of Mycoplasma gallinaceum by PCR amplification of the 16S rRNA gene from respiratory disease in village chickens

P. Ponnusamy, T. Lurthu Reetha, B.S.M. Ronald, B. Puvarajan, R. Manicakm
1Department of Veterinary Microbiology, Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu - 614 625, Tamil Nadu, India.
Cite article:- Ponnusamy P., Reetha Lurthu T., Ronald B.S.M., Puvarajan B., Manicakm R. (2018). Detection of Mycoplasma gallinaceum by PCR amplification of the 16S rRNA gene from respiratory disease in village chickens. Indian Journal of Animal Research. 53(7): 959-962. doi: 10.18805/ijar.B-3604.

ABSTRACT

In the present study, Mycoplasma gallinaceum was detected by PCR amplification of 16S rRNA gene from chronic respiratory disease in village chickens of Cauvery delta region of Tamil Nadu. Necropsy was performed to find out the etiological agent in desi birds mortality. At necropsy, airsacculitis with caseous exudate were found in the thoracic and abdominal cavity. Caseous material from airsacs was collected aseptically from dead birds for detection of Mycoplasma species. DNA was extracted from caseous material by using tissue DNA extraction kit. PCR was carried out using primers to amplify 16S rRNA gene belonging to Mycoplasma species. The amplified product yielded approximately 700-bp length (703 to 713 bp) of the 16S rRNA gene specific for Mycoplasma species. Further, it was subjected to sequence analysis and confirmed as Mycoplasma gallinaceum by NCBI blast analysis.  In the present communication, detection of M. gallinaceum by PCR amplification of 16S rRNA gene provides a powerful tool for rapid diagnosis. 

REFERENCES

  1. Abolnik, C. and Beylefeld, A. (2015). Complete genome sequence of Mycoplasma gallinaceum. Genome Announc, 3:e00712– e00715.
  2. Adeyemi, M., Bwala, D.G., Abolink, C. (2018). Comparative evaluation of the pathogenicity of Mycoplasma gallinaceum in chickens. Avian Diseases, 62(1):50-56
  3. Ahmed, M.A.H. (2016). Epidemiological and diagnostic studies on Mycoplasma gallisepticum and Mycoplasma synoviae originating from poultry and non-poultry birds. Inaugural dissertation for the acquisition of the doctoral degree at the Faculty of Veterinary Medicine Justus-Liebig-University Giessen, Germany.
  4. Bradbury, J.M., Yavari, C.A., Dare, C.M. (2001). Mycoplasmas and respiratory disease in pheasants and partridges. Avian Pathology, 30:391–396. 
  5. Charlton, B.R., Bermudez, A.J., Boulianne, M., Halvorson, D.A., Schrader, J.S., Newman, L.J., Sander, J.E., Wakenell, P.S. (1996). Avian Disease Manual, Carlton B. R. American Association of Avian Pathologist, Kennett Square, Pa, USA.
  6. Ferguson, N.N. and Noormohammadi, H.A. (2013). Mycoplasma synoviae infection. In: Diseases of Poultry Swayne DE. editor, 13th Ed., Wiley-Blackwell, Ames, Iowa, pp 900-906.
  7. García, M., Ikuta, N., Levisohn, S., Kleven, S.H. (2005). Evaluation and comparison of various PCR methods for detection of Mycoplasma gallisepticum infection in chickens. Avian Diseases, 49(1): 125–132. 
  8. Gondal, M.A., Rabbani, M., Muhammad, K., Yaqub, T., Babar, M.E., Sheikh, A.A., Ahmad, A., Shabbir, M.Z., Khan, M.I.(2015). Characterization of Mycoplasma gallisepticum isolated from commercial poultry flocks. The Journal of Animal & Plant Sciences, 25(1): 108-113.
  9. Jordan, F.T., Erno, H., Cottew, S., Hinz, K.H., Stipkovits, L. (1982). Characterization and taxonomic description of five mycoplasma serovars (serotypes) of avian origin and their elevation to species rank and further evaluation of the taxonomic status of Mycoplasrna synoviae. International Journal of Systematic and Evolutionary Microbiology, 32: 108–115.
  10. Kleven, S.H. (2008a). Control of avian Mycoplasma infections in commercial poultry. Avian Diseases, 52: 367-374.
  11. Kleven, S.H. (2008b). Mycoplasmosis. In: A Laboratory Manual for the Isolation, Identification and Characterization of Avian Pathogens. 5th ed. (Dufour-Zavala, L., Swayne, D.E., Glisson, J.R., Pearson, J.E., Reed, W.M., Jackwood, M.W., Woolcock, P.R.) Jacksonville, Florida. 59-64.
  12. Lauerman, L.H., Hoerr, F.J., Sharpton, A.R., Shah, S.M., Van Santen, V.L. (1993). Development and application of a polymerase chain reaction assay for Mycoplasma synoviae. Avian Diseases, 37: 829-834.
  13. Lecis, R., Secci, F., Mandas, L., Muzzeddu, M., Pittau, M., Alberti, A. (2016). Molecular identification and sequence characterization of mycoplasmas in free-living birds of prey. Journal of Zoo and Wildlife Medicine, 47(3):917-922. 
  14. Lierz, M., Hagen, N., Harcourt-Brown, N., Hernandez-Divers, S. J., Lüschow, D., Hafez, H. M. (2007). Prevalence of mycoplasmas in eggs from birds of prey using culture and a genus-specific mycoplasma polymerase chain reaction. Avian Pathology, 36:145–150.
  15. Nascimento, E.R., Yamamoto, R., Herrick, K.R., Tait, R.C. (1991). Polymerase chain reaction for detection of Mycoplasma gallisepticum. Avian Diseases, 35: 62-69.
  16. National Center for Biotechnology Information (NCBI) [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; [1988]. Available from: https://www.ncbi.nlm.nih.gov/ OIE, (2008). 
  17. Avian Mycoplasmosis. OIE Terrestrial Manual, 482-496.
  18. Osman, K.M., Aly, M.M., Amin, Z.M.S., Hasann, B.S. (2009). Mycoplasma gallisepticum: an emerging challenge to the poultry industry in Egypt. Scientific and Technical Review – OIE, 28 (3): 1015-1023.
  19. Pranay, K., Roy, A., Aher T. K. (2018). Characterization of p30 membrane protein gene of Mycoplasma agalactiae isolates by polymerase chain reaction and restriction endonuclease enzyme assay. African Journal of Microbiology Research, 12(14): 333-337.
  20. Raviv, Z. and Ley, D.H. (2013). Mycoplasma gallisepticum infection. In: Diseases of Poultry, 13th Ed. (Swayne DE. Editor). Wiley-Blackwell, Ames, Iowa, pp 877–893.
  21. Rasoulinezhad, S., Bozorgmehrifard, M.H., Hosseini, H., Sheikhi,N., Charkhkar, S. (2017). Molecular detection and phylogenetic analysis of Mycoplasma gallisepticum from backyard and commercial turkey flocks in Iran. Veterinary Research Forum, 8 (4): 293 - 298
  22. Razin, S., Yogev, D., Naot, Y. (1998). Molecular biology and pathogenicity of Mycoplasmas. Microbiology and Molecular Biology Reviews, 62: 1094-1156.
  23. Senthilnathan, G., Shenbagam, S., Suryanarayana, T., Thiyageeswaran, M. (2015). Isolation and molecular confirmation of Mycoplasma synoviae infection from broiler breeder farms in Tamilnadu. Indian Journal of Animal Research, 49 (1): 91-94
  24. Tabatabaei Qomi, R., Sheykh Hasan, M., Fazaely, H., Kalhor, N., Ghiasi, M. (2014). Development of a PCR assay to detect Mycoplasma contamination in cord blood hematopoietic stem cells. Iranian Journal of Microbiology, 6(4): 281–284.
  25. Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., Kumar, S. (2011). MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28: 2731-2739.
  26. Van Kuppeveld, F.J., Van der Logt, J.T., Angulo, A.F., Van Zoest, M.J., Quint, W.G., Niesters, H.G., Galama, J.M., Melchers, W.J. (1992). Genus and species specific identification of Mycoplasmas by 16S rRNA amplification. Applied and Environmental Microbiology, 58: 2606-2615.
  27. Wang, Y., Whithear, K.G., Ghiocas, E. (1990). Isolation of Mycoplasma gallinarum and M. gallinaceum from the reproductive tract of hens. Australian Veterinary Journal, 67: 31–32. 
  28. Yoshida, T., Maeda, S., Deguchi, T., Ishiko, H. (2002). Phylogeny based rapid identification of Mycoplasmas and Ureaplasmas from urethritis patients. Journal of Clinical Microbiology, 40(1): 105–110. 
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