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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 7 (july 2019) : 870-873,   Doi: 10.18805/ijar.B-3603

Effect of dimethyl sulfoxide (DMSO) on preseservabilty of boar semen at 15°c

B
B. Singh
K
K.K. Bonia
M
M.K. Tamuli
S
S. Sinha
S
S. Tamuly
A
A.K. Sarma
G
G. Bhuyan
1Assam Agricultual University, Khanapara, Guwahati-781 022, Assam, India.
Cite article:- Singh B., Bonia K.K., Tamuli M.K., Sinha S., Tamuly S., Sarma A.K., Bhuyan G. (2018). Effect of dimethyl sulfoxide (DMSO) on preseservabilty of boar semen at 15°c. Indian Journal of Animal Research. 53(7): 870-873. doi: 10.18805/ijar.B-3603.

ABSTRACT

A total of  30 ejaculates comprising five from each  of six boars (three Hampshire and three Rani breeds) were collected to study the effect of  DMSO (Dimethyl sulfoxide) on preservability  and fertility  of  boar semen at liquid state at 15°C in ten extenders viz.Androheps+Strepto-penicillin+Gentamycin GEPS+Strepto-penicillin+Gentamycin, Androheps + 1% DMSO, Androheps + 0.7% DMSO, Androheps + Gentamicin sulfate + 0.5% DMSO,Androheps +Strepto-penicillin + 0.5% DMSO, GEPS (Glucose-sodium salt of EDTA-potassium sodium tartrate-sodium citrate dihydrate) + 1% DMSO, GEPS+0.7% DMSO, GEPS+Gentamicin sulfate+0.5% DMSO and GEPS+ Strepto-penicillin + 0.5% DMSO extenders. After holding semen each ejaculate was split into 10 parts and extended (1:3) with the said experimental dilutors and preserved at 15°C in refrigerator up to 168 hr. The evaluation of sperm motility and Hypo-osmotic swelling test (HOST) of preserved semen samples were recorded at 0 (just after extension), 24, 48, 72, 96, 120, 144 and 168 hr as per the standard method. The results indicated that the addition of DMSO in extenders had no significant effect on preservability of boar semen at 15°C.

REFERENCES

  1. Chutia, T.; Biswas, R.K.; Tamuli, M.K.; Sinha, S.; Goswami, J.; Deka, B.C.; Banik, S. and Kayastha, R.B. (2014). Efficacy of different extenders in preservation of liquid Hampshire boar semen at 15°C. Indian Journal of Animal Research. 48(5): 496-500.
  2. Estienne, M.J.; Harper, A.F. and Day, J.L. (2007). Characteristics of sperm motility in boar semen diluted in different extenders and stored for 7 days at 18°C. Reproductive Biology. 7(3): 221-231.
  3. Hafez, B. and Hafes, E.S.E. (2000). In: Reproduction in Farm Animals.7th Edn. Published by Lippincott Willams and Wilkins, a Wolters Kluwer Company, 530 Walnut Street, Philadelphia, Pennsylvania 19106-3621, USA.
  4. Johnson L A, Weitze K F, Fiser P Maxwell, W M C. (2000). Storage of boar semen. Anim Reproduction Science. 62: 143-172.
  5. Kumaresan A, Kadirvel G, Bujarbaruah K M, Bardoloi R K, Das A, Kumar S and Naskar S. (2009). Preservation of boar semen at 18°C induces lipid peroxidation and apoptosis like changes in spermatozoa. Animal Reproduction Science. 110:162-171.
  6. Lalrintluanga K, Deka B C, Nath K C, Hmar L Bhuyan, Dand Biswas R.K. (2016). Effect of different extenders on the quality of boar semen during preservation at 18°C.nternational Journal of Multidisciplinary Approach and Studies. 03(1): 224-232.
  7. Tallilepzuk (1998). Preservation of boar spermatozoa with special reference to acquisition of cold shock resistance. M.V.Sc. Thesis, Assam Agricultural University, Khanapara, Guwahati-22.
  8. Tamuli M K. (1982). Studies on semen characteristics and artificial insemination in pigs. M.V.Sc. Thesis, Assam Agricultural University, Khanapara, Guwahati-22.
  9. Tamuli M K and Das A.(2009). Preservation of boar semen for artificial insemination.Winter School on “Livelihood security through sustainable pig production”, 25 November to 15 December, National Research Centre on Pig, ICAR, Rani, Guwahati, 12-15 pp.
  10. Tamuli M K and Rajkonwar C K.(1987). Innovation of boar semen dilutors. Indian Veterinary Journal. 64:782-784.
  11. Tamuli M K, Rajkonwar C K and Goswami B K. (1986). Preservation of boar semen. Indian Journal of Animal Reproduction.7(2): 70-72.
  12. Weitze K F. (1990). Long-term storage of extended boar semen. Reproduction of Domestic Animal Supplementary 1:231- 253.
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 7 (july 2019) : 870-873,   Doi: 10.18805/ijar.B-3603

    Effect of dimethyl sulfoxide (DMSO) on preseservabilty of boar semen at 15°c

    B
    B. Singh
    K
    K.K. Bonia
    M
    M.K. Tamuli
    S
    S. Sinha
    S
    S. Tamuly
    A
    A.K. Sarma
    G
    G. Bhuyan
    1Assam Agricultual University, Khanapara, Guwahati-781 022, Assam, India.
    Cite article:- Singh B., Bonia K.K., Tamuli M.K., Sinha S., Tamuly S., Sarma A.K., Bhuyan G. (2018). Effect of dimethyl sulfoxide (DMSO) on preseservabilty of boar semen at 15°c. Indian Journal of Animal Research. 53(7): 870-873. doi: 10.18805/ijar.B-3603.

    ABSTRACT

    A total of  30 ejaculates comprising five from each  of six boars (three Hampshire and three Rani breeds) were collected to study the effect of  DMSO (Dimethyl sulfoxide) on preservability  and fertility  of  boar semen at liquid state at 15°C in ten extenders viz.Androheps+Strepto-penicillin+Gentamycin GEPS+Strepto-penicillin+Gentamycin, Androheps + 1% DMSO, Androheps + 0.7% DMSO, Androheps + Gentamicin sulfate + 0.5% DMSO,Androheps +Strepto-penicillin + 0.5% DMSO, GEPS (Glucose-sodium salt of EDTA-potassium sodium tartrate-sodium citrate dihydrate) + 1% DMSO, GEPS+0.7% DMSO, GEPS+Gentamicin sulfate+0.5% DMSO and GEPS+ Strepto-penicillin + 0.5% DMSO extenders. After holding semen each ejaculate was split into 10 parts and extended (1:3) with the said experimental dilutors and preserved at 15°C in refrigerator up to 168 hr. The evaluation of sperm motility and Hypo-osmotic swelling test (HOST) of preserved semen samples were recorded at 0 (just after extension), 24, 48, 72, 96, 120, 144 and 168 hr as per the standard method. The results indicated that the addition of DMSO in extenders had no significant effect on preservability of boar semen at 15°C.

    REFERENCES

    1. Chutia, T.; Biswas, R.K.; Tamuli, M.K.; Sinha, S.; Goswami, J.; Deka, B.C.; Banik, S. and Kayastha, R.B. (2014). Efficacy of different extenders in preservation of liquid Hampshire boar semen at 15°C. Indian Journal of Animal Research. 48(5): 496-500.
    2. Estienne, M.J.; Harper, A.F. and Day, J.L. (2007). Characteristics of sperm motility in boar semen diluted in different extenders and stored for 7 days at 18°C. Reproductive Biology. 7(3): 221-231.
    3. Hafez, B. and Hafes, E.S.E. (2000). In: Reproduction in Farm Animals.7th Edn. Published by Lippincott Willams and Wilkins, a Wolters Kluwer Company, 530 Walnut Street, Philadelphia, Pennsylvania 19106-3621, USA.
    4. Johnson L A, Weitze K F, Fiser P Maxwell, W M C. (2000). Storage of boar semen. Anim Reproduction Science. 62: 143-172.
    5. Kumaresan A, Kadirvel G, Bujarbaruah K M, Bardoloi R K, Das A, Kumar S and Naskar S. (2009). Preservation of boar semen at 18°C induces lipid peroxidation and apoptosis like changes in spermatozoa. Animal Reproduction Science. 110:162-171.
    6. Lalrintluanga K, Deka B C, Nath K C, Hmar L Bhuyan, Dand Biswas R.K. (2016). Effect of different extenders on the quality of boar semen during preservation at 18°C.nternational Journal of Multidisciplinary Approach and Studies. 03(1): 224-232.
    7. Tallilepzuk (1998). Preservation of boar spermatozoa with special reference to acquisition of cold shock resistance. M.V.Sc. Thesis, Assam Agricultural University, Khanapara, Guwahati-22.
    8. Tamuli M K. (1982). Studies on semen characteristics and artificial insemination in pigs. M.V.Sc. Thesis, Assam Agricultural University, Khanapara, Guwahati-22.
    9. Tamuli M K and Das A.(2009). Preservation of boar semen for artificial insemination.Winter School on “Livelihood security through sustainable pig production”, 25 November to 15 December, National Research Centre on Pig, ICAR, Rani, Guwahati, 12-15 pp.
    10. Tamuli M K and Rajkonwar C K.(1987). Innovation of boar semen dilutors. Indian Veterinary Journal. 64:782-784.
    11. Tamuli M K, Rajkonwar C K and Goswami B K. (1986). Preservation of boar semen. Indian Journal of Animal Reproduction.7(2): 70-72.
    12. Weitze K F. (1990). Long-term storage of extended boar semen. Reproduction of Domestic Animal Supplementary 1:231- 253.
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