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Research Article

volume 53 issue 7 (july 2019) : 979-983,   Doi: 10.18805/ijar.B-3597

Characterisation of KiSS1R gene and Non genetic factors affecting reproductive traits in Indian goats

S
Siddhant Sekhar Sahoo
C
Chinmoy Mishra
S
Stuti Tanaya Mohanty
R
Rakesh Kaushik
P
P.K. Rout
M
M.K. Singh
G
Gopal Dass
K
Kamdev Sethy
K
Kumaresh Behera
M
M.S. Dige
1ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura-281 122, Uttar Pradesh, India.
Cite article:- Sahoo Sekhar Siddhant, Mishra Chinmoy, Mohanty Tanaya Stuti, Kaushik Rakesh, Rout P.K., Singh M.K., Dass Gopal, Sethy Kamdev, Behera Kumaresh, Dige M.S. (2019). Characterisation of KiSS1R gene and Non genetic factors affecting reproductive traits in Indian goats. Indian Journal of Animal Research. 53(7): 979-983. doi: 10.18805/ijar.B-3597.

ABSTRACT

This study was undertaken to explore the genetic polymorphism in the KiSS1R (GPR54) gene from 80 Black Bengal, 50 Ganjam and 20 Raighar goat. Each of the sampled goats was recorded for its reproductive traits. The genomic DNA was isolated from the collected blood samples. The target 3’ UTR comprising of 246 bp fragment of KiSS1R gene was successfully amplified using the specific primer. Amplified samples were subjected for HRM analysis followed by sequencing. The nucleotide sequence alignment with the retrieved DNA sequence from NCBI BLAST confirmed absence of polymorphic pattern in KiSS1R gene in 3’ UTR. However, in the studied populations breed had significant effect on littersize, kidding interval and age at sexual maturity. It was found that age at sexual maturity and kidding interval were the highest in Ganjam goat population as compared to Raighar and Black Bengal goat population. Litter size was found highest in Black Bengal goat. 

REFERENCES

  1. Ahlawat, S., Sharma, R., Maitra, A., Tantia, M.S. and Prakash, V. (2015). Association analysis of a novel SNP in GPR54 gene with reproductive traits in Indian goats. Indian Journal of Dairy Science, 68 (1): 39-44.
  2. Ali, S.Z., Hogue, M.M. and Haanath, M.A. (1973). A study on growth and reproductive performance of Black Bengal goats under farm conditions. Indian Veterinary Journal, 50: 438-440.
  3. Cao, G.L., Chu, M.X., Fang, L., Feng, T., Di, R. and Li, N. (2011). Analysis on DNA sequence of GPR54 gene and its association with litter size in goats. Molecular Biology Reports, 38: 3839-3848.
  4. Das, A.K., Manna, M.K., Sahoo, A.K., Deb, R., Paul, R.K. (2008). Studies on reproductive traits of Bengal goats in a part of West Bengal. Indian Journal of Small Ruminants, 14(2): 259-261.
  5. Faruque, S., Chowdhury, S.A., Siddiquee, N.U., Afroz, M.A. (2010). Performance and genetic parameters of economically important traits of Black Bengal goat. Journal of Bangladesh Agricultural University, 8(1): 67-78.
  6. Greives, T.J., Mason, A.O., Scotti, M.A., Levine, J., Ketterson, E.D., Kriegsfeld, L.J., Demas, G.E. (2006). Environmental control of Kisspeptin implications for seasonal reproduction. Endocrinology, 148: 1158-1166.
  7. Han, S.K., Gottsch, M.L., Lee, K.J., Popa, S.M., Smith, J.T., Jakawich, S.K., Clifton, D.K., Steiner, R.A., Herbison, A.E. (2005). Activation of gonadotropin-releasing hormone neurons by kisspeptin as a neuroendocrine switch for the onset of puberty. Journal of Neuroscience, 25: 11349-11356.
  8. Irwig, M.S., Fraley, G.S., Smith, J.T., Acohido, B.V., Popa, S.M., Cunningham, M.J., et al. (2004). Kisspeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS-1 mRNA in the male rat. Neuroendocrinology, 80: 264-272.
  9. Kotani, M., Detheux, M., Vandenbogaerde, A., Communi, D., Vanderwinden, J.M., Le Poul, E., et al (2001). The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. Journal of Biological Chemistry 276 (37): 34631-34316. 
  10. Kuohung, W., Kaiser, U.B. (2006). GPR54 and KiSS-1: role in the regulation of puberty and reproduction.Reviews in Endocrine and Metabolic Disorders7: 257-263. 
  11. Madeli, U.C. (1977). Inheritance of some production traits in a flock of Ganjam goats. M.V.Sc. Thesis, Faculty of Veterinary Science and Animal Husbandry, O.U.A.T., Bhubaneswar, Odisha, India.
  12. Maitra, A., Sharma, R., Ahlawar, S., Tantia, M.S. (2014). Novel genetic polymorphisms in caprine GPR54 gene associated with reproductive functions. Indian Journal of Animal Science, 84 (11): 1196-1201.
  13. Matoulkova, E., Michalova, E., Vojtesek, B., Hrstka, R. (2012). The role of the 3' untranslated region in posttranscriptional regulation of protein expression in mammalian cells. RNA Biology, 9(5): 563-576.
  14. Messager, S., Chatzidaki, E.E., Ma, D., Hendrick, A.G., Zahn, D., Dixon, J., Thresher, R.R.,et al. (2005). Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54. Proceedings of the National Academy of Sciences of the United States of America, 102: 1761-1766.
  15. Mohanty, G.P., Mohapatra, A.K., Dash, S.K., Sahoo, G. (2006). Study of management practices and performance of Kalahandi goats in Orissa. Indian Journal of Animal Production and Management, 20(1-4): 92-93.
  16. Mukherjee, R., Koyal, A., Banerjee, P.K. (2014). Studies on morphological and morphometric variation of Black Bengal goat (Capra hircus bengalensis) in some villages of Hooghly and Howrah districts, West Bengal. International Journal of Multidisciplinary Approach and Studies, 1(6): 284-294.
  17. Myenuddin, M., and Wahab, M.A. (1989). Reproductive performance of Black Bengal Goats in Manikganj, Bangladesh. Veterinarian, 6(1): 48-49. 
  18. Ohtaki T, Shintani Y, Honda S, Matsumoto H, Hori A, Kanehashi K, Terao Y, Kumano S, et al. (2001). Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor. Nature, 411(6837):613-7.
  19. Parhar, I.S., Ogawa, S., and Sakuma, Y. (2004). Laser-captured single digoxigenin labeled neurons of gonadotropin-releasing hormone types reveal a novel G protein-coupled receptor (Gpr54) during maturation in cichlid fish. Endocrinology, 145: 3613-3618.
  20. Pompolo, S., Pereira, A., Estrada, K.M., and Clarke, I.J. (2006). Co-localization of kisspeptin and gonadotropin-releasing hormone in the ovine brain. Endocrinology, 147: 804-810.
  21. Rahman, A.M., Hossain, A., Ahmed, M.U., and Sen, M.M. (1977). Studies on some reproductive performance and biometry of the female genital tract of Black Bengal goats. Indian Journal of Animal Sciences, 47(11): 724-725.
  22. Rao, P.K., Dash, S.K., Patro, B.N., and Nayak, S. (2002). Studies on Ganjam, Black Bengal goats and their crosses found in Orissa. Indian Journal of Animal Production and Management, 18(3-4): 135-138.
  23. Rao, P.K., Dash, S.K., Singh, M.K., Rai, B., and Singh, N.P. (2009). Ganjam goat of Orissa and its management practices. Indian Journal of Small Ruminants, 15 (1): 44-50.
  24. Roa, J., Aguilar, E., Dieguez, C., Pinilla, L., Tena-Sempere, M. (2008). New frontiers in kisspeptin/GPR54 physiology as fundamental gatekeepers of reproductive function. Frontiers in Neuroendocrinology, 29: 48-69.
  25. Sabapara, G.P., Deshpande, S.B., Singh, G., and Joshi, B.K. (2010).Reproduction and production performance of Surti goats in its native tract. Indian Journal of Small Ruminants, 16(2):195-198.
  26. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning a Laboratory Manual, Cold Spring Harbour Lab Press, Cold Spring Harbour, NY.
  27. Shibata, M., Friedman, R.L., Ramaswamy, S., and Plant, T.M. (2007). Evidence that down regulation of hypothalamic KiSS-1 expression is involved in the negative feedback action of testosterone to regulate luteinising hormone secretion in the adult male rhesus monkey (Macacamulatta). Journal of Neuroendocrinology, 19: 432-438.
  28. Singh Bariha SP, Rao P.K, Patro B. N, Dash S. K and Panda P (2008) Genetic analysis of indigenous goat of keonjhar district of Orissa. Indian Veterinary Journal, 85: 843-845.
  29. Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis Version 6.0. Molecular Biology and Evolution, 30(12): 2725-2729.
  30. Tena-Sempere, M. (2006). GPR54 and kisspeptin in reproduction. Human Reproduction Update, 12: 631-639.
  31. Wittwer, C.T., Herrmann, M.G., Moss, A.A., and Rasmussen, R.P. (1997). Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques, 22: 130-131.
  32. Zeshmarani, S., Dhara, K.C., Samanta, A.K., Samanta, R., and Majumder, S.C. (2007). Reproductive performance of goats in Eastern and North-eastern India. Livestock Research Rural Development, 19: 114-117. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 7 (july 2019) : 979-983,   Doi: 10.18805/ijar.B-3597

    Characterisation of KiSS1R gene and Non genetic factors affecting reproductive traits in Indian goats

    S
    Siddhant Sekhar Sahoo
    C
    Chinmoy Mishra
    S
    Stuti Tanaya Mohanty
    R
    Rakesh Kaushik
    P
    P.K. Rout
    M
    M.K. Singh
    G
    Gopal Dass
    K
    Kamdev Sethy
    K
    Kumaresh Behera
    M
    M.S. Dige
    1ICAR-Central Institute for Research on Goats, Makhdoom, Farah, Mathura-281 122, Uttar Pradesh, India.
    Cite article:- Sahoo Sekhar Siddhant, Mishra Chinmoy, Mohanty Tanaya Stuti, Kaushik Rakesh, Rout P.K., Singh M.K., Dass Gopal, Sethy Kamdev, Behera Kumaresh, Dige M.S. (2019). Characterisation of KiSS1R gene and Non genetic factors affecting reproductive traits in Indian goats. Indian Journal of Animal Research. 53(7): 979-983. doi: 10.18805/ijar.B-3597.

    ABSTRACT

    This study was undertaken to explore the genetic polymorphism in the KiSS1R (GPR54) gene from 80 Black Bengal, 50 Ganjam and 20 Raighar goat. Each of the sampled goats was recorded for its reproductive traits. The genomic DNA was isolated from the collected blood samples. The target 3’ UTR comprising of 246 bp fragment of KiSS1R gene was successfully amplified using the specific primer. Amplified samples were subjected for HRM analysis followed by sequencing. The nucleotide sequence alignment with the retrieved DNA sequence from NCBI BLAST confirmed absence of polymorphic pattern in KiSS1R gene in 3’ UTR. However, in the studied populations breed had significant effect on littersize, kidding interval and age at sexual maturity. It was found that age at sexual maturity and kidding interval were the highest in Ganjam goat population as compared to Raighar and Black Bengal goat population. Litter size was found highest in Black Bengal goat. 

    REFERENCES

    1. Ahlawat, S., Sharma, R., Maitra, A., Tantia, M.S. and Prakash, V. (2015). Association analysis of a novel SNP in GPR54 gene with reproductive traits in Indian goats. Indian Journal of Dairy Science, 68 (1): 39-44.
    2. Ali, S.Z., Hogue, M.M. and Haanath, M.A. (1973). A study on growth and reproductive performance of Black Bengal goats under farm conditions. Indian Veterinary Journal, 50: 438-440.
    3. Cao, G.L., Chu, M.X., Fang, L., Feng, T., Di, R. and Li, N. (2011). Analysis on DNA sequence of GPR54 gene and its association with litter size in goats. Molecular Biology Reports, 38: 3839-3848.
    4. Das, A.K., Manna, M.K., Sahoo, A.K., Deb, R., Paul, R.K. (2008). Studies on reproductive traits of Bengal goats in a part of West Bengal. Indian Journal of Small Ruminants, 14(2): 259-261.
    5. Faruque, S., Chowdhury, S.A., Siddiquee, N.U., Afroz, M.A. (2010). Performance and genetic parameters of economically important traits of Black Bengal goat. Journal of Bangladesh Agricultural University, 8(1): 67-78.
    6. Greives, T.J., Mason, A.O., Scotti, M.A., Levine, J., Ketterson, E.D., Kriegsfeld, L.J., Demas, G.E. (2006). Environmental control of Kisspeptin implications for seasonal reproduction. Endocrinology, 148: 1158-1166.
    7. Han, S.K., Gottsch, M.L., Lee, K.J., Popa, S.M., Smith, J.T., Jakawich, S.K., Clifton, D.K., Steiner, R.A., Herbison, A.E. (2005). Activation of gonadotropin-releasing hormone neurons by kisspeptin as a neuroendocrine switch for the onset of puberty. Journal of Neuroscience, 25: 11349-11356.
    8. Irwig, M.S., Fraley, G.S., Smith, J.T., Acohido, B.V., Popa, S.M., Cunningham, M.J., et al. (2004). Kisspeptin activation of gonadotropin releasing hormone neurons and regulation of KiSS-1 mRNA in the male rat. Neuroendocrinology, 80: 264-272.
    9. Kotani, M., Detheux, M., Vandenbogaerde, A., Communi, D., Vanderwinden, J.M., Le Poul, E., et al (2001). The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. Journal of Biological Chemistry 276 (37): 34631-34316. 
    10. Kuohung, W., Kaiser, U.B. (2006). GPR54 and KiSS-1: role in the regulation of puberty and reproduction.Reviews in Endocrine and Metabolic Disorders7: 257-263. 
    11. Madeli, U.C. (1977). Inheritance of some production traits in a flock of Ganjam goats. M.V.Sc. Thesis, Faculty of Veterinary Science and Animal Husbandry, O.U.A.T., Bhubaneswar, Odisha, India.
    12. Maitra, A., Sharma, R., Ahlawar, S., Tantia, M.S. (2014). Novel genetic polymorphisms in caprine GPR54 gene associated with reproductive functions. Indian Journal of Animal Science, 84 (11): 1196-1201.
    13. Matoulkova, E., Michalova, E., Vojtesek, B., Hrstka, R. (2012). The role of the 3' untranslated region in posttranscriptional regulation of protein expression in mammalian cells. RNA Biology, 9(5): 563-576.
    14. Messager, S., Chatzidaki, E.E., Ma, D., Hendrick, A.G., Zahn, D., Dixon, J., Thresher, R.R.,et al. (2005). Kisspeptin directly stimulates gonadotropin-releasing hormone release via G protein-coupled receptor 54. Proceedings of the National Academy of Sciences of the United States of America, 102: 1761-1766.
    15. Mohanty, G.P., Mohapatra, A.K., Dash, S.K., Sahoo, G. (2006). Study of management practices and performance of Kalahandi goats in Orissa. Indian Journal of Animal Production and Management, 20(1-4): 92-93.
    16. Mukherjee, R., Koyal, A., Banerjee, P.K. (2014). Studies on morphological and morphometric variation of Black Bengal goat (Capra hircus bengalensis) in some villages of Hooghly and Howrah districts, West Bengal. International Journal of Multidisciplinary Approach and Studies, 1(6): 284-294.
    17. Myenuddin, M., and Wahab, M.A. (1989). Reproductive performance of Black Bengal Goats in Manikganj, Bangladesh. Veterinarian, 6(1): 48-49. 
    18. Ohtaki T, Shintani Y, Honda S, Matsumoto H, Hori A, Kanehashi K, Terao Y, Kumano S, et al. (2001). Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor. Nature, 411(6837):613-7.
    19. Parhar, I.S., Ogawa, S., and Sakuma, Y. (2004). Laser-captured single digoxigenin labeled neurons of gonadotropin-releasing hormone types reveal a novel G protein-coupled receptor (Gpr54) during maturation in cichlid fish. Endocrinology, 145: 3613-3618.
    20. Pompolo, S., Pereira, A., Estrada, K.M., and Clarke, I.J. (2006). Co-localization of kisspeptin and gonadotropin-releasing hormone in the ovine brain. Endocrinology, 147: 804-810.
    21. Rahman, A.M., Hossain, A., Ahmed, M.U., and Sen, M.M. (1977). Studies on some reproductive performance and biometry of the female genital tract of Black Bengal goats. Indian Journal of Animal Sciences, 47(11): 724-725.
    22. Rao, P.K., Dash, S.K., Patro, B.N., and Nayak, S. (2002). Studies on Ganjam, Black Bengal goats and their crosses found in Orissa. Indian Journal of Animal Production and Management, 18(3-4): 135-138.
    23. Rao, P.K., Dash, S.K., Singh, M.K., Rai, B., and Singh, N.P. (2009). Ganjam goat of Orissa and its management practices. Indian Journal of Small Ruminants, 15 (1): 44-50.
    24. Roa, J., Aguilar, E., Dieguez, C., Pinilla, L., Tena-Sempere, M. (2008). New frontiers in kisspeptin/GPR54 physiology as fundamental gatekeepers of reproductive function. Frontiers in Neuroendocrinology, 29: 48-69.
    25. Sabapara, G.P., Deshpande, S.B., Singh, G., and Joshi, B.K. (2010).Reproduction and production performance of Surti goats in its native tract. Indian Journal of Small Ruminants, 16(2):195-198.
    26. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning a Laboratory Manual, Cold Spring Harbour Lab Press, Cold Spring Harbour, NY.
    27. Shibata, M., Friedman, R.L., Ramaswamy, S., and Plant, T.M. (2007). Evidence that down regulation of hypothalamic KiSS-1 expression is involved in the negative feedback action of testosterone to regulate luteinising hormone secretion in the adult male rhesus monkey (Macacamulatta). Journal of Neuroendocrinology, 19: 432-438.
    28. Singh Bariha SP, Rao P.K, Patro B. N, Dash S. K and Panda P (2008) Genetic analysis of indigenous goat of keonjhar district of Orissa. Indian Veterinary Journal, 85: 843-845.
    29. Tamura, K., Stecher, G., Peterson, D., Filipski, A., and Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis Version 6.0. Molecular Biology and Evolution, 30(12): 2725-2729.
    30. Tena-Sempere, M. (2006). GPR54 and kisspeptin in reproduction. Human Reproduction Update, 12: 631-639.
    31. Wittwer, C.T., Herrmann, M.G., Moss, A.A., and Rasmussen, R.P. (1997). Continuous fluorescence monitoring of rapid cycle DNA amplification. Biotechniques, 22: 130-131.
    32. Zeshmarani, S., Dhara, K.C., Samanta, A.K., Samanta, R., and Majumder, S.C. (2007). Reproductive performance of goats in Eastern and North-eastern India. Livestock Research Rural Development, 19: 114-117. 
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