Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 7 (july 2019) : 847-851,   Doi: 10.18805/ijar.B-3591

Polymorphism of beta (b) casein gene and their association with milk production traits in Sahiwal and HF crossbred cattle

A
Akhilesh Pandey
M
M.S. Thakur
Y
Y. Pandey
1Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.
Cite article:- Pandey Akhilesh, Thakur M.S., Pandey Y. (2019). Polymorphism of beta (b) casein gene and their association with milk production traits in Sahiwal and HF crossbred cattle. Indian Journal of Animal Research. 53(7): 847-851. doi: 10.18805/ijar.B-3591.

ABSTRACT

Present research work was carried out on 50 Sahiwal and 50 HF Crossbred cattle in the Department of Animal Genetics and Breeding of College of Veterinary Science and A H Jabalpur. During the research work the PCR amplified products of 121bp was digested by restriction endonuclease enzyme DdeI, which recognizes G^AATTC sites. The patterns evolved in the present study showed that the presence of one restriction site on one alleles and absence of restriction site on other alleles resulted in the appearance of three bands of 121, 86 and 35bp.This genotype was referred to as A1A2. When both strand had no restriction sites for the enzyme, only one band of 121bp was observed on the gel such genotype was designated as A2A2 type. The â- casein gene showed A2A2 and A1A2 genotypes were observed in Sahiwal and HF crossbred cattle. The genotypic frequencies of â-casein (CSN2)/ DdeI gene for A1A1, A1A2 and A2A2 are 0.00, 0.30, and 0.70 in Sahiwal and 0.00, 0.64 and 0.36 in HF crossbred cattle, respectively and the gene frequency A1 and A2 is 0.15 and 0.85 in Sahiwal and 0.32 and 0.68 in HF crossbred cattle. High frequency of A2 allele was observed in both the breeds of cattle under the study.

REFERENCES

  1. Bell, J.S., Gregory, T.G. and Andrew, J.C.,(2006). Health implications of milk containing â-Casein with the A2 genetic variant. Food Sciences and Nutrition, 46: 93-100.
  2. Cardak, A.D., (2005). Effects of genetic variants in milk protein on yield and composition of milk from Holstein-Friesian and Simmentaler 35 (1): 41- 47.
  3. Farrel, H.M.R, Jimenez, F., Bleck, G.T., Brown, E.M., Butler, J.E. and Creamer, L.K., (2004). Nomenclature of the proteins of cows’ milk - sixth revision. Journal of Dairy Sciences, 87: 1641-1674.
  4. Hanusova, E., Huba, J., Oravcov, M.P., Polak, I. and Vrtkova, I., (2010). Genetic variants of beta-casein in Holstein dairy cattle in Slovakia. Slovak Journal of Animal Science, 43 (2): 63 – 66.
  5. Ikonen, T., Bovenhuis, H., Ojala, M., Ruottinen, O. and Georges, M., (2001). Associations between casein haplotypes and first lactation milk production traits in Finnish Ayrshire cows. Journal of Dairy Science, 84: 507-514.
  6. John, S.W., Weitzner, G., Rozen, R. and Scriver, C.R., (1991). A rapid procedure for extracting genomic DNA from leukocytes. Nucleic Acid Research, 19 (2): 408.
  7. Malarmathi, M., Kumar, T.M.A.S., Parthiban, M., Muthuramalingam, T. and Palanisammi, A., (2014). Analysis of â-casein gene for A1 and A2 genotype using allele specific PCR in Kangeyam and Holstein Friesian crossbred cattle in Tamilnadu. Indian Journal of Veterinary & Animal Science Research, 43 (4): 310–315.
  8. Mir, N.S., Ullah, O. and Sheikh, R., (2014). Genetic polymorphism of milk protein variants and their association studies with milk yield in Sahiwal cattle. African Journal of Biotechnology, 13 (4): 555-565.
  9. Miluchova, M., Gabor, M. and Trakovicka, A., (2013). Analysis of genetic structure in Slovak Pinzgau cattle using five candidate genes related to milk production traits. Genetika, 46 (3): 865-875.
  10. Molee, A., Duanghaklang, N. and Na-Lampang, P., (2011). Effects of AcylCoA: diacyl-glycerol acyl-transferase-1 (DGAT1) gene on milk production traits in crossbred Holstein dairy cattle. Tropical Animal Health and Production, 44 (4): 751-755. 
  11. Nilsen, H., Olsen, H.G., Hayes, B., Sehested, E., Svendsen, M., Nome, T., Meuwissen, T. and Lien, S., (2009). Casein haplotypes and their association with milk production traits in Norwegian Red cattle. Genetic Selection and Evolution, 41: 24-27.
  12. Ramesha, K.P., Rao, A., Basavaraju, M., Alex, R., Kataktalware, M.A., Jeyakumar, S. and Varalakshmi, S. (2016). Genetic variants of â-casein in cattle and buffalo breeding bulls in Karnataka state of India. Indian Journal of Biotechnology, 15: 178-181.
  13. Shende, T.C., Kulkarni, P.S. and Pawar, P.C. (2017). Genotyping of HF crossbred cattle for â-casein genes using PCR-RFLP. Indian Research Journal of Extension Education (special issue on Veterinary Research & Extension), 17: 105-107.
  14. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Method. 8th edn. The lowa State College Press, Inc. Amer. lowa USA. p.950 
  15. Truswell, A.S. (2005). The A2 milk case. European Journal of Clinical Nutrition, 59: 623–631.
  16. Yeh, F.C., Yang, R.C., Boyle, T.B.J., Ye, Z.H. and Mao, J.X. (1999). Popgene 32 version 1.32, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada. 
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 53 issue 7 (july 2019) : 847-851,   Doi: 10.18805/ijar.B-3591

    Polymorphism of beta (b) casein gene and their association with milk production traits in Sahiwal and HF crossbred cattle

    A
    Akhilesh Pandey
    M
    M.S. Thakur
    Y
    Y. Pandey
    1Department of Animal Genetics and Breeding, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, Jabalpur-482 001, Madhya Pradesh, India.
    Cite article:- Pandey Akhilesh, Thakur M.S., Pandey Y. (2019). Polymorphism of beta (b) casein gene and their association with milk production traits in Sahiwal and HF crossbred cattle. Indian Journal of Animal Research. 53(7): 847-851. doi: 10.18805/ijar.B-3591.

    ABSTRACT

    Present research work was carried out on 50 Sahiwal and 50 HF Crossbred cattle in the Department of Animal Genetics and Breeding of College of Veterinary Science and A H Jabalpur. During the research work the PCR amplified products of 121bp was digested by restriction endonuclease enzyme DdeI, which recognizes G^AATTC sites. The patterns evolved in the present study showed that the presence of one restriction site on one alleles and absence of restriction site on other alleles resulted in the appearance of three bands of 121, 86 and 35bp.This genotype was referred to as A1A2. When both strand had no restriction sites for the enzyme, only one band of 121bp was observed on the gel such genotype was designated as A2A2 type. The â- casein gene showed A2A2 and A1A2 genotypes were observed in Sahiwal and HF crossbred cattle. The genotypic frequencies of â-casein (CSN2)/ DdeI gene for A1A1, A1A2 and A2A2 are 0.00, 0.30, and 0.70 in Sahiwal and 0.00, 0.64 and 0.36 in HF crossbred cattle, respectively and the gene frequency A1 and A2 is 0.15 and 0.85 in Sahiwal and 0.32 and 0.68 in HF crossbred cattle. High frequency of A2 allele was observed in both the breeds of cattle under the study.

    REFERENCES

    1. Bell, J.S., Gregory, T.G. and Andrew, J.C.,(2006). Health implications of milk containing â-Casein with the A2 genetic variant. Food Sciences and Nutrition, 46: 93-100.
    2. Cardak, A.D., (2005). Effects of genetic variants in milk protein on yield and composition of milk from Holstein-Friesian and Simmentaler 35 (1): 41- 47.
    3. Farrel, H.M.R, Jimenez, F., Bleck, G.T., Brown, E.M., Butler, J.E. and Creamer, L.K., (2004). Nomenclature of the proteins of cows’ milk - sixth revision. Journal of Dairy Sciences, 87: 1641-1674.
    4. Hanusova, E., Huba, J., Oravcov, M.P., Polak, I. and Vrtkova, I., (2010). Genetic variants of beta-casein in Holstein dairy cattle in Slovakia. Slovak Journal of Animal Science, 43 (2): 63 – 66.
    5. Ikonen, T., Bovenhuis, H., Ojala, M., Ruottinen, O. and Georges, M., (2001). Associations between casein haplotypes and first lactation milk production traits in Finnish Ayrshire cows. Journal of Dairy Science, 84: 507-514.
    6. John, S.W., Weitzner, G., Rozen, R. and Scriver, C.R., (1991). A rapid procedure for extracting genomic DNA from leukocytes. Nucleic Acid Research, 19 (2): 408.
    7. Malarmathi, M., Kumar, T.M.A.S., Parthiban, M., Muthuramalingam, T. and Palanisammi, A., (2014). Analysis of â-casein gene for A1 and A2 genotype using allele specific PCR in Kangeyam and Holstein Friesian crossbred cattle in Tamilnadu. Indian Journal of Veterinary & Animal Science Research, 43 (4): 310–315.
    8. Mir, N.S., Ullah, O. and Sheikh, R., (2014). Genetic polymorphism of milk protein variants and their association studies with milk yield in Sahiwal cattle. African Journal of Biotechnology, 13 (4): 555-565.
    9. Miluchova, M., Gabor, M. and Trakovicka, A., (2013). Analysis of genetic structure in Slovak Pinzgau cattle using five candidate genes related to milk production traits. Genetika, 46 (3): 865-875.
    10. Molee, A., Duanghaklang, N. and Na-Lampang, P., (2011). Effects of AcylCoA: diacyl-glycerol acyl-transferase-1 (DGAT1) gene on milk production traits in crossbred Holstein dairy cattle. Tropical Animal Health and Production, 44 (4): 751-755. 
    11. Nilsen, H., Olsen, H.G., Hayes, B., Sehested, E., Svendsen, M., Nome, T., Meuwissen, T. and Lien, S., (2009). Casein haplotypes and their association with milk production traits in Norwegian Red cattle. Genetic Selection and Evolution, 41: 24-27.
    12. Ramesha, K.P., Rao, A., Basavaraju, M., Alex, R., Kataktalware, M.A., Jeyakumar, S. and Varalakshmi, S. (2016). Genetic variants of â-casein in cattle and buffalo breeding bulls in Karnataka state of India. Indian Journal of Biotechnology, 15: 178-181.
    13. Shende, T.C., Kulkarni, P.S. and Pawar, P.C. (2017). Genotyping of HF crossbred cattle for â-casein genes using PCR-RFLP. Indian Research Journal of Extension Education (special issue on Veterinary Research & Extension), 17: 105-107.
    14. Snedecor, G.W. and Cochran, W.G. (1994). Statistical Method. 8th edn. The lowa State College Press, Inc. Amer. lowa USA. p.950 
    15. Truswell, A.S. (2005). The A2 milk case. European Journal of Clinical Nutrition, 59: 623–631.
    16. Yeh, F.C., Yang, R.C., Boyle, T.B.J., Ye, Z.H. and Mao, J.X. (1999). Popgene 32 version 1.32, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada. 
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)