Effect of optimization of the levels of dissolved oxygen in semen extender on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa

DOI: 10.18805/ijar.B-3585    | Article Id: B-3585 | Page : 704-710
Citation :- Effect of optimization of the levels of dissolved oxygen in semen extender on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa.Indian Journal Of Animal Research.2019.(53):704-710
Abhishek Kumar, J.K. Prasad, A.R. Mustapha, R. Katiyar, G.K. Das, S.K. Ghosh and M.R. Verma abhiawadhesarita@gmail.com
Address : Division of Animal Reproduction, Division of Livestock Economics and Statistics, ICAR-Indian Veterinary Research Institute, Izatnagar - 243 122, Bareilly, Uttar Pradesh, India.
Submitted Date : 1-03-2018
Accepted Date : 25-04-2018

Abstract

The objective of present study was to investigate the effects of two different levels of dissolved oxygen (DO) i.e. 4 and 8 ppm in the extenders on physico-morphological attributes and functional membrane integrity of crossbred bull spermatozoa following cryopreservation. Only those ejaculates that had a mass activity of ³ 3+ and individual progressive motility of  ³ 70% were utilized. Each of the ejaculates were divided and extended to have 80×106 spermatozoa/ mL of extender in three groups i.e. Group I (control), Group II (4 ppm) and Group III (8 ppm). Progressive motility (%) was higher (p<0.05) in Group II and III as compared to Group I at both pre-freeze and post-thaw stage. Per cent live sperm and HOST reactive sperm were higher (p<0.05) in Group II and in Group II and III as compared to Group I at pre-freeze and post-thaw stage, respectively. Sperm abnormality (%) was lower (p<0.05) in Group II at post-thaw stage. Per cent acrosome intact sperm did not differ (p>0.05) among the groups at pre-freeze stage, but, were higher (p<0.05) in Group II and III as compared to Group I at post-thaw stage. In conclusion, cryopreservation of semen having DO level of 4 ppm in semen extender yielded better results followed by 8 ppm in terms of semen quality parameters and functional membrane integrity of crossbred bull spermatozoa.

Keywords

Dissolved oxygen Functional membrane integrity Physico-morphological attributes Spermatozoa.

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