Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 53 issue 6 (june 2019) : 807-813

Bacterial fingerprinting of faecal samples of pigs supplemented with plant sourced feed additives
 

A.K. Samanta, J. Chikkerur, A.P. Kolte, A. Dhali, P.K. Javvaji, Sohini Roy, S. Senani, Manpal Sridhar
1ICAR - National Institute of Animal Nutrition and Physiology, Adugodi, Hosur Road, Bengaluru – 560 030, Karnataka, India.
Cite article:- Samanta A.K., Chikkerur J., Kolte A.P., Dhali A., Javvaji P.K., Roy Sohini, Senani S., Sridhar Manpal (2018). Bacterial fingerprinting of faecal samples of pigs supplemented with plant sourced feed additives. Indian Journal of Animal Research. 53(6): 807-813. doi: 10.18805/ijar.B-3582.

ABSTRACT

The present study was aimed to see the faecal bacterial fingerprints of pigs applying terminal restriction fragment length polymorphism (T-RFLP) analysis. Sixteen crossbred (Large White Yorkshire X desi) grower pigs (body weight of 15.8±1.1 kg), were divided into four groups (control, antibiotic, herbal residue and prebiotic) with four in each treatment. T-RFLP analysis revealed comparable bacterial abundance in control - antibiotic and herbal residue - prebiotic groups. Abundance of Peptostreptococcus anaerobius (an antibiotic resistant pathogen) was higher in antibiotic supplemented pigs. Number of significantly responded operational taxonomic units (OTU) was higher in herbal residue and prebiotic supplemented pigs than control or antibiotic pigs. Bacterial abundance was significantly higher in pigs supplemented with prebiotic, followed by herbal residue, antibiotic, and control. The abundance of beneficial bacteria such as Sarcina maxima, Bacteroidetes sp., Cetobacterium somerae, Selenomonas sputigera, Fecalibacterium prausnitzi were significantly higher in prebiotic groups. The present findings established that herbal residue and prebiotic are alternative to feed antibiotic in pigs. 

REFERENCES

  1. Bosscher, D., Van Loo, J. and Franck, A. (2006). Inulin and oligofructose as prebiotics in the prevention of intestinal infections and diseases. Nutrition Research Reviews, 19: 216-26. 
  2. Castanon, J.I.R. (2007). History of the use of antibiotics as growth promoters in European poultry feeds. Poultry Science, 86: 2466-    2471. 
  3. Cilieborg, M.S., Boye, M., Molbak, L., Thymann, T. and Sangild, P.T. (2011). Preterm birth and necrotizing enterocolitis alter gut colonization in pigs. Pediatric Research , 69: 10-16.
  4. Clarke, K.R. and Warwick, R.M. (2001). Changes in marine communities: an approach to statistical analysis and interpretation. 2nd ed. Primer E Ltd, Plymouth United Kingdom.
  5. Costa, E., Puhl, N.J., Selinger, L.B. and Inglis, G.D. (2009). Characterization of mucosa associated bacterial communities of the mouse intestine by terminal restriction fragment length polymorphism: utility of sampling strategies and methods to reduce single stranded DNA artefacts. Journal of Microbiological Methods, 78: 175-180.
  6. De Lange, C.F.M., Pluske, J., Gong, J. and Nyachoti, C.M. (2010). Strategic use of feed ingredients and feed additives to stimulate gut health and development in young pigs. Livestock Science, 134: 124-134. 
  7. Duncan, S.H., Flint, H.J. and Stewart, C.S. (1998). Inhibitory activity of gut bacteria against Escherichia coli O157mediated by dietary plant metabolites. FEMS Microbiology Letters, 164: 283-288.
  8. Gao, Q., Xiao, C., Min, M., Zhang, C., Peng, S. and Shi, Z. (2016). Effects of probiotics dietary supplementation on growth performance, innate immunity and digestive enzymes of silver pomfret, Pampus argenteus. Indian Journal of Animal Research, 50: 936-941.
  9. Hashemi, S.R. and Davoodi, H. (2011). Herbal plants and their derivatives as growth and health promoters in animal nutrition. Veterinary Research Communications, 35: 169-180. 
  10. Ivarsson, E., Liu, H.Y., Dicksved, J., Roos, S. and Lindberg, J.E. (2012). Impact of chicory inclusion in a cereal-based diet on digestibility, organ size and faecal microbiota in growing pigs. Animal, 6: 1077-1085. 
  11. Khan, M.T., van Dijl, J.M. and Harmsen, H.J. (2014). Antioxidants keep the potentially probiotic but highly oxygen-sensitive human gut bacterium Faecalibacterium prausnitzii alive at ambient air. PLoS One, 9: e96097.
  12. Kupfer, D.G. and Canale-Parola, E. (1967). Pyruvate metabolism in Sarcina maxima. Journal of Bacteriology, 94: 984-990.
  13. Lamendella, R., Domingo, J.W., Ghosh, S., Martinson, J. and Oerther, D.B. (2011). Comparative fecal metagenomics unveils unique functional capacity of the swine gut. BMC Microbiology, 11: 103.
  14. Larsen, A.M., Mohammed, H.H. and Arias, C.R. (2014). Characterization of the gut microbiota of three commercially valuable warm water fish species. Journal of Applied Microbiology, 116: 1396-1404.
  15. Laverock, B., Smith, C.J., Tait, K., Osborn, A.M., Widdicombe, S. and Gilbert, J.A. (2010). Bioturbating shrimp after the structure and diversity of bacterial communities in coastal marine sediments. The ISME Journal, 4: 1531-1544.
  16. Leser, T.D., Lindecrona, R.H., Jensen, T.K., Jensen, B.B. and Moller, K. (2000). Changes in bacterial community structure in the colon of pigs fed different experimental diets and after infection. Applied and Environmental Microbiology, 66: 3290-3296.
  17. Liang, T., Li, X., Du, J., Yao, W., Sun, G., Dong, X., Liu, Z., Ou, J., Meng, Q., Gu, W. and Wang, W. (2011). Identification and isolation of a spiroplasma pathogen from diseased freshwater prawns, Macrobrachium rosenbergii in China: A new freshwater crustacean host. Aquaculture, 318: 1-6.
  18. Liu, W., Marsh, T.L., Cheng, H. and Forney, L.J. (1997). Characterization of microbial diversity by determining terminal restriction fragment length polymorphism of genes encoding 16S rRNA. Applied and Environmental Microbiology, 63: 4516-4522.
  19. Liu, H., Ivarsson, E., Dicksved, J., Lundh, T. and Lindberg, J.E. (2012). Inclusion of chicory (Cichorium intybus L.) in pigs’ diets affects the intestinal microenvironment and the gut microbiota. Applied and Environmental Microbiology , 78: 4102-4109.
  20. Natalja, S. (2014). Molecular quantitative analysis of human intestinal microbiota in different age groups. Master’s thesis. http://    www.tymri.ut.ee/sites/default/files/tymri/2014_sebunova_natalja.pdf. 
  21. Pedersen, R., Andersen, A.D., Molbak, L., Stagsted, J. and Boye, M. (2013). Changes in the gut microbiota of cloned and non-cloned control pigs during development of obesity: gut microbiota during development of obesity in cloned pigs. BMC Microbiology, 13: 30. 
  22. R Development Core Team. (2008). A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. ISBN 3-900051-07-0, URL http://www.R-project.org.
  23. Roberfroid, M. (2007). Prebiotics: the concept revisited. Journal of Nutrition, 137: 830S-837S.
  24. Roberts, S.A., Shore, K.P., Paviour, S.D., Holland, D. and Morris, A.J. (2006). Antimicrobial susceptibility of anaerobic bacteria in New Zealand: 1999-2003. Journal of Antimicrobial Chemotherapy, 57: 992-998. 
  25. Samanta, A.K., Torok, V.A., Percy, N.J., Abimosleh, S.M. and Howarth, G.S. (2012). Microbial fingerprinting detects unique bacterial communities in the faecal microbiota of rats with experimentally induced colitis. Journal of Microbiology, 50: 218-225. 
  26. Samanta, A.K., Jayarapal, N., Senani, S., Kolte, A.P. and Sridhar, M. (2013). Prebiotic inulin: Useful dietary adjuncts to manipulate the livestock gut microflora. Brazilian Journal of Microbiology, 44: 1-14. 
  27. Sawicki, C.M., Livingston, K.A., Obin, M., Roberts, S.B., Chung, M. and McKeown, N.M. (2017). Dietary fiber and human gut microbiota: application of evidence mapping methodology. Nutrients, 9:125.
  28. Schutte, U.M., Abdo, Z., Bent, S.J., Shyu, C., Williams, C.J., Pierson, J.D. and Fomey, I.J. (2008). Advances in the use of terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes to characterize microbial communities. Applied Microbiology and Biotechnology, 80: 365-380.
  29. Shyu, C., Soule, T., Bent, S.J., Foster, J.A. and Forney, L.J. (2007). MiCA: a web based tool for the analysis of microbial communities based on terminal restriction fragment length polymorphism of 16S and 18S rRNA genes. Microbial Ecology , 53: 562-570.
  30. Torok, V.A., Ophel-Keller, K., Loo, M. and Hughes, R.J. (2008). Application of methods for identifying broiler chicken gut bacterial species linked with increased energy metabolism. Applied and Environmental Microbiology, 74: 783-791. 
  31. Torsvik, V., Goksoyr, J. and Daae, F.L. (1990). High diversity in DNA of soil bacteria. Applied and Environmental Microbiology, 56: 782-787.
  32. Troy, E.B. and Kasper, D.L. (2010). Beneficial effects of Bacteroides fragilis polysaccharides on the immune system. Frontiers in Bioscience, 15: 25-34.
  33. Turner, J.L., Pas, S., Dritz, S., Minton, J.E. (2002). Review: Alternatives to conventional Antimicrobials in Swine Diets. The Professional Animal Scientist, 17: 217-726.
  34. Van Loo, J. (2007). How chicory fructans contribute to zoo technical performance and well-being in livestock and companion animals. Journal of Nutrition, 137: 2594S-97S.
  35. Yoo, S.Y., Kim, K.J., Lim, S.H., Kim, K.W., Hwang, H.K., Min, B.M., Choe, S.J. and Kook, J.K. (2005). First isolation of Streptococcus downei from human dental plaques. FEMS Microbioogy Letters,249: 323-326.
  36. Zargar, M.A., Pathak, A.K. and Daing, M.I. (2017). Screening and evaluation of antioxidant and antimicrobial properties of condensed tannins containing tree leaves of Jammu province. Indian Journal of Animal Research, 51: 110-1104.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)