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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 6 (june 2019) : 716-719,   Doi: 10.18805/ijar.B-3576

Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen

I
I. Borgohain
D
D. Dutta
P
P. Borah
D
D. Borah
1Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara-781 022, Assam, India.
Cite article:- Borgohain I., Dutta D., Borah P., Borah D. (2018). Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen. Indian Journal of Animal Research. 53(6): 716-719. doi: 10.18805/ijar.B-3576.

ABSTRACT

The present study was conducted for molecular evaluation of bull semen and to study its relationship with different semen characteristics. Fresh semen samples were collected from six breeding bulls. A total of six ejaculates were collected from each of six healthy breeding Jersey bulls at 4 days interval. Immediately after collection, the samples were subjected to physio-morphological and biomolecular evaluation. Percentage of Hypo-osmotic Swelling Test (HOST) positive spermatozoa and acrosome-intact sperms increased with an increase in initial sperm motility. Expression of Chaperonin Containing T-Complex protein 1, subunit 8 (CCT8) gene was found to be negatively correlated with sperm motility, whereas the expression of  Adenylate Kinase 1 (AK1) gene did not show any significant relationship with sperm motility.

REFERENCES

  1. Arangasamy, A.; Kasimanickam, V.R.; DeJarnette, J.M. and Kasimanickam, R.K. (2011). Association of CRISP2, CCT8, PEBP1 mRNA abundance in sperm and sire conception rate in Holstein bulls. Theriogenology., 76: 570-577
  2. Barik, A.K.; Mohanty, B.N.; Ray, S.K.H. and Mohanty, D.N. (1987). Fructose and citric acid concentration and its relation with seminal attributes in bull semen. Indian J. Anim. Repod., 8(2): 84-87.
  3. Branton, C.; C. B. James; T. E. Patrick and M. H. Newsom. (1951). The relationship between certain semen quality tests and fertility and the interrelationship of these tests. J. Dairy Sci., 34: 310-316.
  4. Gomez-Puertas, P.; Martin-Benito, J.; Carrascosa, J.L.; Willison, K.R. and Valpuesta, J.M. (2004). The substrate recognition mechanisms in chaperonins. Journal of Molecular Recognition., 17: 85–94.
  5. Janssen, E.; Dzeja, P.P.; Oerlemans, F.; Simonetti, A.W.; Heerschap, A.; de Haan, A.; Rush, P.S.; Terjung, R.R.; Wieringa, B. and Terzic, A.(2000). Adenylate kinase 1 gene deletion disrupts muscle energetic economy despite metabolic rearrangement. Embo J., 19:6371–6381
  6. Kasimanickam, V; Kasimanickama, R; Arangasamya, A.; Saberivanda, A.; Stevensond, J.S. and Kastelice J.P.(2012). Association between mRNA abundance of functional sperm function proteins and fertility of Holstein bulls. Theriogenology., 78: 2007-2019
  7. Leroux, M.R. and Hartl, F.U. (2000). Versatility of the cytosolic chaperonin TRiC/CCT, Curr. Biol., 10: 260–264
  8. Pucar, D.; Janssen, E.; Dzeja, P.P.; Juranic, N.; Macura, S.; Wieringa, B. and Terzic, A.(2000). Compromised energetics in the adenylate kinase AK1 gene knockout heart under metabolic stress. J Biol Chem., 275:41424–41429.
  9. Schoff, P.K.; Cheetham, J.and Lardy, H.A. (1989) Adenylate kinase activity in ejaculated bovine sperm flagella. J Biol Chem., 264:6086–    6091. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 6 (june 2019) : 716-719,   Doi: 10.18805/ijar.B-3576

    Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen

    I
    I. Borgohain
    D
    D. Dutta
    P
    P. Borah
    D
    D. Borah
    1Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara-781 022, Assam, India.
    Cite article:- Borgohain I., Dutta D., Borah P., Borah D. (2018). Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen. Indian Journal of Animal Research. 53(6): 716-719. doi: 10.18805/ijar.B-3576.

    ABSTRACT

    The present study was conducted for molecular evaluation of bull semen and to study its relationship with different semen characteristics. Fresh semen samples were collected from six breeding bulls. A total of six ejaculates were collected from each of six healthy breeding Jersey bulls at 4 days interval. Immediately after collection, the samples were subjected to physio-morphological and biomolecular evaluation. Percentage of Hypo-osmotic Swelling Test (HOST) positive spermatozoa and acrosome-intact sperms increased with an increase in initial sperm motility. Expression of Chaperonin Containing T-Complex protein 1, subunit 8 (CCT8) gene was found to be negatively correlated with sperm motility, whereas the expression of  Adenylate Kinase 1 (AK1) gene did not show any significant relationship with sperm motility.

    REFERENCES

    1. Arangasamy, A.; Kasimanickam, V.R.; DeJarnette, J.M. and Kasimanickam, R.K. (2011). Association of CRISP2, CCT8, PEBP1 mRNA abundance in sperm and sire conception rate in Holstein bulls. Theriogenology., 76: 570-577
    2. Barik, A.K.; Mohanty, B.N.; Ray, S.K.H. and Mohanty, D.N. (1987). Fructose and citric acid concentration and its relation with seminal attributes in bull semen. Indian J. Anim. Repod., 8(2): 84-87.
    3. Branton, C.; C. B. James; T. E. Patrick and M. H. Newsom. (1951). The relationship between certain semen quality tests and fertility and the interrelationship of these tests. J. Dairy Sci., 34: 310-316.
    4. Gomez-Puertas, P.; Martin-Benito, J.; Carrascosa, J.L.; Willison, K.R. and Valpuesta, J.M. (2004). The substrate recognition mechanisms in chaperonins. Journal of Molecular Recognition., 17: 85–94.
    5. Janssen, E.; Dzeja, P.P.; Oerlemans, F.; Simonetti, A.W.; Heerschap, A.; de Haan, A.; Rush, P.S.; Terjung, R.R.; Wieringa, B. and Terzic, A.(2000). Adenylate kinase 1 gene deletion disrupts muscle energetic economy despite metabolic rearrangement. Embo J., 19:6371–6381
    6. Kasimanickam, V; Kasimanickama, R; Arangasamya, A.; Saberivanda, A.; Stevensond, J.S. and Kastelice J.P.(2012). Association between mRNA abundance of functional sperm function proteins and fertility of Holstein bulls. Theriogenology., 78: 2007-2019
    7. Leroux, M.R. and Hartl, F.U. (2000). Versatility of the cytosolic chaperonin TRiC/CCT, Curr. Biol., 10: 260–264
    8. Pucar, D.; Janssen, E.; Dzeja, P.P.; Juranic, N.; Macura, S.; Wieringa, B. and Terzic, A.(2000). Compromised energetics in the adenylate kinase AK1 gene knockout heart under metabolic stress. J Biol Chem., 275:41424–41429.
    9. Schoff, P.K.; Cheetham, J.and Lardy, H.A. (1989) Adenylate kinase activity in ejaculated bovine sperm flagella. J Biol Chem., 264:6086–    6091. 
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