Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen

DOI: 10.18805/ijar.B-3576    | Article Id: B-3576 | Page : 716-719
Citation :- Expression study of fertility related genes with respect to sperm motility, HOST and acrosome integrity in bovine semen.Indian Journal Of Animal Research.2019.(53):716-719
I. Borgohain, D. Dutta, P. Borah and D. Borah
Address : Department of Animal Biotechnology, College of Veterinary Science, Assam Agricultural University, Khanapara-781 022, Assam, India.
Submitted Date : 12-02-2018
Accepted Date : 10-07-2018


The present study was conducted for molecular evaluation of bull semen and to study its relationship with different semen characteristics. Fresh semen samples were collected from six breeding bulls. A total of six ejaculates were collected from each of six healthy breeding Jersey bulls at 4 days interval. Immediately after collection, the samples were subjected to physio-morphological and biomolecular evaluation. Percentage of Hypo-osmotic Swelling Test (HOST) positive spermatozoa and acrosome-intact sperms increased with an increase in initial sperm motility. Expression of Chaperonin Containing T-Complex protein 1, subunit 8 (CCT8) gene was found to be negatively correlated with sperm motility, whereas the expression of  Adenylate Kinase 1 (AK1) gene did not show any significant relationship with sperm motility.


Adenylate Kinase 1(AK1) Breeding bulls Hypo-osmotic Swelling Test.


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