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Research Article

volume 53 issue 5 (may 2019) : 675-679,   Doi: 10.18805/ijar.B-3555

Effect of in-ovo injection of glucose and egg white protein on the production performance and gut histomorphometry of broiler chicken

P
P. Kanagaraju
S
S.Rathnapraba
1Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, Tamil Nadu, India.
Cite article:- Kanagaraju P., S.Rathnapraba (2018). Effect of in-ovo injection of glucose and egg white protein on the production performance and gut histomorphometry of broiler chicken. Indian Journal of Animal Research. 53(5): 675-679. doi: 10.18805/ijar.B-3555.

ABSTRACT

A biological trial was carried out to investigate the effect of in ovo injection of glucose and egg white protein on the production performance and gut histomorphometry of broilers. 500 hatching eggs with uniform weight (68±1g) were randomly allotted to five treatment groups each with five replicates of 20 eggs each and incubated. The eggs were injected with one of the following nutrient solutions on 18th day of incubation viz. 0.5 ml of 25 % glucose (T3), 0.5 ml of 12% egg white protein (T4), 0.5 ml of 25 % glucose + egg white protein 12% (T5) into the amnion along this100 eggs served as non injected control (T1) and 100 eggs injected with normal saline (T2) which also served as sham control. The hole was sealed and the period of incubation was continued till 21st day. After hatching, 400 chicks were randomized in to five treatments of five replicates each with 16 chicks. Data on hatchability, hatch weight, bi-weekly body weight, weight gain, feed consumption, FCR and villi height, width, crypt depth of duodenum, jejunum and ileum were recorded and analysed statistically for significant difference. Results revealed that in ovo injection of glucose, egg white protein and their combination significantly (P<0.05) increased hatchability, chick weight, body weight and livability. However, the feed consumption was significantly (P<0.01) higher in glucose, egg white protein and glucose + egg white protein injected birds compared to non injected control and sham groups. The FCR was significantly (P<0.05) improved in all in ovo injected groups compared to control and sham. In ovo feeding significantly (P<0.05) increased the duodenal, jejuna and ileal villi height, width and crypt depth (P<0.01) compared to control birds. In ovo injection of glucose and EWP either alone or in combination improved the productive performance, gut histomorphology and net profit of the broilers.

REFERENCES

  1. Bhattacharyya, A., Majumdar, B., Bhanja, S.K. Mandal, A.B. Dash, B.B. Agarwal, S.K. (2007). Effect of in ovo injection of glucose on growth, immunocompetence and development of digestive organs in turkey poults. Proceedings of the 16th European Symposium on Poultry Nutrition; Strasbourg. France. p.147-150.
  2. BIS (2007) Bureau of Indian Standards of poultry feed. Manak Bhawan, 9, Bahadur Shah Zafar Marg, New Delhi, India.
  3. Chen, W., Wang, R., Xiong, X.L., Wan, H.F., Xu, J., Peng, J. (2010). Influence of in ovo injection of disaccharides, glutamine and â-    hydroxy-â-methylbutyrate on the development of small intestine in duck embryos and neonates, British Journal of Poultry Science, 51:592-601.
  4. Ferket, P.R. and Uni, Z. (2002). Early enteric development of turkeys. Proceedings of the 25th technical turkey conference held at the Shrigley Hall Hotel on 24-26 April, 2002, pp. 59-64.
  5. Foye, O.T., Uni, Z., Fekret, P.R. (2006) Effect of in ovo feeding egg white protein, â-Hydroxy-â-Methyl butyrate and carbohydrates on and State glycogen status and neonatal growth of turkeys. Poultry Science, 85:1185-1192.
  6. Isa Coskun, Guray Erener, Ahmet Sahin, Ufuk Karadavut, Aydýn Altop, Aylin Agma Okur (2014). Impacts of in ovo feeding of DL-    methionine on hatchability and chick weight. Turkish Journal of Agriculture Food Science and Technology, 2: 47-50.
  7. Jia, C.L., Wei, Z.H., Yu, M., Wang, X.Q., Yu, F. (2011). Effect of in ovo feeding maltose on the embryo growth and intestine development of broiler chicken. Indian. J. Anim. Sci., 81: 503–506.
  8. Kanagaraju, P. (2014). Effect of in ovo feeding and early chick nutritional supplement on growth performance of hybrid broiler chicken. Ph.D thesis submitted to Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu.
  9. Kanagaraju, P., Babu, M., Asha Rajini, R., Ramesh, R., Balakrishnan, V. (2015). Effect of in-ovo injection of glucose, glutamine and egg white protein on the production performance and gut histomorphology of hybrid broiler chicken. Proceedings of 9th International Poultry Show and Seminar, 19 to 21 February, 2015, Dhaka, Bangladesh.
  10. Leitao, R.A., Leandro, N.S.M., Cafe, M.B., Stringhini, J.H., Pedroso, A.A., Chaves, L.S. (2008). Inoculation of glucose in ovo of broiler breeders/eggs: incubation parameters and initial performance. Ciencia Animal Brasileira., 9:847-855.
  11. Maiorka, A., Santin, E., Dahlke, F., Boleli, I.C., Furlan, R.L., Macari, M. (2003). Post hatching water and feed deprivation affect the gastrointestinal tract and intestinal mucosa development of broiler chicks. Journal of Applied Poultry Research, 12:483-492.
  12. Miller Carin, R. (2007) Developmental gene expression of nutrient transporters in the small intestine of chickens from lines divergently selected for high or low juvenile body weight. M.Sc., Thesis, Virginia Polytechnic Institute University.
  13. Noor, S.M., Husband, A.J., Widders, P.R. (1995) In ovo vaccination with Campylobacter jejuni establishes early development of intestinal immunity in chickens. British journal of Poultry Science, 36:563-573.
  14. Saki, A., Haghighat, M., Khajali, F. (2013). Supplemental arginine administered in ovo or in the feed reduces the susceptibility of broilers to pulmonary hypertension syndrome. British Journal of Poultry Science, 54:575–580.
  15. Salmanzadeh, M. (2012) The effects of in-ovo injection of glucose on hatchability, Hatching weight and subsequent performance of newly-hatched chicks. Brazilian Journal of Poultry Science, 14:137-140.
  16. Salmanzadeh, M., Yahya Ebrahimnezhad, Habib Aghdam Shahryar and Alireza Lotfi (2011).The effects of in ovo injection of L-    threonine in broiler breeder eggs on characters of hatching and growth performance broiler chickens European Journal of Experimental Biology, 1:164-168.
  17. Santos, T.T., Corzo, A., Kidd, M.T., McDaniel, C.D., Torres Filho, R.A., Araujo, L.F., (2010). Influence of in ovo inoculation with various nutrients and egg size on broiler performance. Journal of Applied Poultry Research, 19, 1-12.
  18. Smirnov, A., Tako, E., Ferket, P.R., Uni, Z. (2006). Mucin gene expression and mucin content in the chicken intestinal goblet cells are affected by in ovo feeding of carbohydrates. Poultry Science, 85:669–673.
  19. SPSS (1999) Statistical software package for the social sciences SPSS, version 17. Int.,USA
  20. Steel, R.G.D. and Torrie, J.H. (1981) Principles and Procedures of Statistics: A biometrical approach. 2nd. Ed. McGraw-Hill, Singapore. 
  21. Tako, E., Ferket, P.R., Uni, Z. (2004). Effects of in ovo feeding of carbohydrates and beta-hydroxy-beta-methylbutyrate on the development    of chicken intestine. Poult. Sci., 83:2023-2028. 
  22. Uni Z. and Yahav, S. (2009) Managing prenatal development of broiler chickens to improve productivity and thermo tolerance. In: Greenwood P., Bell A., Vercoe P., Viljoen G. (eds) Managing the prenatal environment to enhance livestock productivity. Springer, Dordrecht
  23. Uni, Z., Ferket, P.R., Tako, E., Kedar, O. (2005) In ovo feeding improves energy status of late-term chicken embryos. Poultry Science, 84:764-770. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 5 (may 2019) : 675-679,   Doi: 10.18805/ijar.B-3555

    Effect of in-ovo injection of glucose and egg white protein on the production performance and gut histomorphometry of broiler chicken

    P
    P. Kanagaraju
    S
    S.Rathnapraba
    1Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai-600 007, Tamil Nadu, India.
    Cite article:- Kanagaraju P., S.Rathnapraba (2018). Effect of in-ovo injection of glucose and egg white protein on the production performance and gut histomorphometry of broiler chicken. Indian Journal of Animal Research. 53(5): 675-679. doi: 10.18805/ijar.B-3555.

    ABSTRACT

    A biological trial was carried out to investigate the effect of in ovo injection of glucose and egg white protein on the production performance and gut histomorphometry of broilers. 500 hatching eggs with uniform weight (68±1g) were randomly allotted to five treatment groups each with five replicates of 20 eggs each and incubated. The eggs were injected with one of the following nutrient solutions on 18th day of incubation viz. 0.5 ml of 25 % glucose (T3), 0.5 ml of 12% egg white protein (T4), 0.5 ml of 25 % glucose + egg white protein 12% (T5) into the amnion along this100 eggs served as non injected control (T1) and 100 eggs injected with normal saline (T2) which also served as sham control. The hole was sealed and the period of incubation was continued till 21st day. After hatching, 400 chicks were randomized in to five treatments of five replicates each with 16 chicks. Data on hatchability, hatch weight, bi-weekly body weight, weight gain, feed consumption, FCR and villi height, width, crypt depth of duodenum, jejunum and ileum were recorded and analysed statistically for significant difference. Results revealed that in ovo injection of glucose, egg white protein and their combination significantly (P<0.05) increased hatchability, chick weight, body weight and livability. However, the feed consumption was significantly (P<0.01) higher in glucose, egg white protein and glucose + egg white protein injected birds compared to non injected control and sham groups. The FCR was significantly (P<0.05) improved in all in ovo injected groups compared to control and sham. In ovo feeding significantly (P<0.05) increased the duodenal, jejuna and ileal villi height, width and crypt depth (P<0.01) compared to control birds. In ovo injection of glucose and EWP either alone or in combination improved the productive performance, gut histomorphology and net profit of the broilers.

    REFERENCES

    1. Bhattacharyya, A., Majumdar, B., Bhanja, S.K. Mandal, A.B. Dash, B.B. Agarwal, S.K. (2007). Effect of in ovo injection of glucose on growth, immunocompetence and development of digestive organs in turkey poults. Proceedings of the 16th European Symposium on Poultry Nutrition; Strasbourg. France. p.147-150.
    2. BIS (2007) Bureau of Indian Standards of poultry feed. Manak Bhawan, 9, Bahadur Shah Zafar Marg, New Delhi, India.
    3. Chen, W., Wang, R., Xiong, X.L., Wan, H.F., Xu, J., Peng, J. (2010). Influence of in ovo injection of disaccharides, glutamine and â-    hydroxy-â-methylbutyrate on the development of small intestine in duck embryos and neonates, British Journal of Poultry Science, 51:592-601.
    4. Ferket, P.R. and Uni, Z. (2002). Early enteric development of turkeys. Proceedings of the 25th technical turkey conference held at the Shrigley Hall Hotel on 24-26 April, 2002, pp. 59-64.
    5. Foye, O.T., Uni, Z., Fekret, P.R. (2006) Effect of in ovo feeding egg white protein, â-Hydroxy-â-Methyl butyrate and carbohydrates on and State glycogen status and neonatal growth of turkeys. Poultry Science, 85:1185-1192.
    6. Isa Coskun, Guray Erener, Ahmet Sahin, Ufuk Karadavut, Aydýn Altop, Aylin Agma Okur (2014). Impacts of in ovo feeding of DL-    methionine on hatchability and chick weight. Turkish Journal of Agriculture Food Science and Technology, 2: 47-50.
    7. Jia, C.L., Wei, Z.H., Yu, M., Wang, X.Q., Yu, F. (2011). Effect of in ovo feeding maltose on the embryo growth and intestine development of broiler chicken. Indian. J. Anim. Sci., 81: 503–506.
    8. Kanagaraju, P. (2014). Effect of in ovo feeding and early chick nutritional supplement on growth performance of hybrid broiler chicken. Ph.D thesis submitted to Tamil Nadu Veterinary and Animal Sciences University, Chennai, Tamil Nadu.
    9. Kanagaraju, P., Babu, M., Asha Rajini, R., Ramesh, R., Balakrishnan, V. (2015). Effect of in-ovo injection of glucose, glutamine and egg white protein on the production performance and gut histomorphology of hybrid broiler chicken. Proceedings of 9th International Poultry Show and Seminar, 19 to 21 February, 2015, Dhaka, Bangladesh.
    10. Leitao, R.A., Leandro, N.S.M., Cafe, M.B., Stringhini, J.H., Pedroso, A.A., Chaves, L.S. (2008). Inoculation of glucose in ovo of broiler breeders/eggs: incubation parameters and initial performance. Ciencia Animal Brasileira., 9:847-855.
    11. Maiorka, A., Santin, E., Dahlke, F., Boleli, I.C., Furlan, R.L., Macari, M. (2003). Post hatching water and feed deprivation affect the gastrointestinal tract and intestinal mucosa development of broiler chicks. Journal of Applied Poultry Research, 12:483-492.
    12. Miller Carin, R. (2007) Developmental gene expression of nutrient transporters in the small intestine of chickens from lines divergently selected for high or low juvenile body weight. M.Sc., Thesis, Virginia Polytechnic Institute University.
    13. Noor, S.M., Husband, A.J., Widders, P.R. (1995) In ovo vaccination with Campylobacter jejuni establishes early development of intestinal immunity in chickens. British journal of Poultry Science, 36:563-573.
    14. Saki, A., Haghighat, M., Khajali, F. (2013). Supplemental arginine administered in ovo or in the feed reduces the susceptibility of broilers to pulmonary hypertension syndrome. British Journal of Poultry Science, 54:575–580.
    15. Salmanzadeh, M. (2012) The effects of in-ovo injection of glucose on hatchability, Hatching weight and subsequent performance of newly-hatched chicks. Brazilian Journal of Poultry Science, 14:137-140.
    16. Salmanzadeh, M., Yahya Ebrahimnezhad, Habib Aghdam Shahryar and Alireza Lotfi (2011).The effects of in ovo injection of L-    threonine in broiler breeder eggs on characters of hatching and growth performance broiler chickens European Journal of Experimental Biology, 1:164-168.
    17. Santos, T.T., Corzo, A., Kidd, M.T., McDaniel, C.D., Torres Filho, R.A., Araujo, L.F., (2010). Influence of in ovo inoculation with various nutrients and egg size on broiler performance. Journal of Applied Poultry Research, 19, 1-12.
    18. Smirnov, A., Tako, E., Ferket, P.R., Uni, Z. (2006). Mucin gene expression and mucin content in the chicken intestinal goblet cells are affected by in ovo feeding of carbohydrates. Poultry Science, 85:669–673.
    19. SPSS (1999) Statistical software package for the social sciences SPSS, version 17. Int.,USA
    20. Steel, R.G.D. and Torrie, J.H. (1981) Principles and Procedures of Statistics: A biometrical approach. 2nd. Ed. McGraw-Hill, Singapore. 
    21. Tako, E., Ferket, P.R., Uni, Z. (2004). Effects of in ovo feeding of carbohydrates and beta-hydroxy-beta-methylbutyrate on the development    of chicken intestine. Poult. Sci., 83:2023-2028. 
    22. Uni Z. and Yahav, S. (2009) Managing prenatal development of broiler chickens to improve productivity and thermo tolerance. In: Greenwood P., Bell A., Vercoe P., Viljoen G. (eds) Managing the prenatal environment to enhance livestock productivity. Springer, Dordrecht
    23. Uni, Z., Ferket, P.R., Tako, E., Kedar, O. (2005) In ovo feeding improves energy status of late-term chicken embryos. Poultry Science, 84:764-770. 
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