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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 2 (february 2019) : 275-277,   Doi: 10.18805/ijar.B-3487

Cryptosporidium oocyst shedding in buffalo calves in Haryana: A case study

K
Krutanjali Swain
A
Abhilash Routray
S
Saraswat Sahoo
S
Subha Ganguly
1Department of Veterinary Parasitology, Faculty of Veterinary Science and Animal Husbandry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, India.
Cite article:- Swain Krutanjali, Routray Abhilash, Sahoo Saraswat, Ganguly Subha (2018). Cryptosporidium oocyst shedding in buffalo calves in Haryana: A case study. Indian Journal of Animal Research. 53(2): 275-277. doi: 10.18805/ijar.B-3487.

ABSTRACT

Bovine cryptosporidiosisis primarily associated with neonatal diarrhoea with higher morbidity than mortality in young calves till they attain immunological maturity. The present investigation relates to a report on the shedding of Cryptosporidium oocyst in two buffalo calves of buffalo farm, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar from 1st days up to 3 month of age at 15 days interval using simple conventional microscopy. By using formol-ether concentration technique followed by modified Ziehl-Neelsen (ZN) acid fast staining, Cryptosporidium oocysts were concentrated and identified. The Cryptosporidium oocysts appeared as reddish pink coloured bodies against a bluish/greenish coloured background at oil immersion using ZN staining kit. The maximum oocyst shedding was observed (2.3 oocyst / field) during 16 to 30 days of age. The oocyst shedding gradually decreased with increase in age afterwards clearly indicating the disease of young buffalo calves.

REFERENCES

  1. Bhagat, M., Sood, S., Yadav, A., Katoch, R., Chakraborty, D., Godara, R., Sultana, M. and Sangha, N. (2017). Clinico-haematological studies on experimental Cryptosporidium parvum Jammu isolate infection in Swiss albino mice. Indian J. Anim. Res. 51(5): 1-6. DOI: 10.18805/ijar.B-3318
  2. Bhat, S.A., Juyal, P.D. and Singla, L.D. (2012). Prevalence of cryptosporidiosis in neonatal buffalo calves in Ludhiana district of Punjab, India. Asian J. Anim. Vet. Adv. 7(6): 512-520.
  3. Bhat, S.A., Juyal, P.D. and Singla, L.D. (2013). Bovine cryptosporidiosis: brief review of its distribution in India. Trends Parasitol. Res. 2(2): 5-13.
  4. Desai, N.T., Sarkar, R. And Kang, G. (2012). Cryptosporidiosis: an under-recognized public health problem. Trop. Parasitol. 2(2): 91-98.
  5. Fayer, R., Morgan, U. and, Upton, S.J. (2000). Epidemiology of Cryptosporidium: transmission, detection and identification. Int. J. Parasitol. 30: 1305–1322.
  6. Fayer, R. and Xiao, L. (2008). Cryptosporidium and cryptosporidiosis, 2nd ed. CRC press, Boca Raton, UK. pp. 560.
  7. Gupta, S.K. and Singla, L.D. (2012). Diagnostic trends in parasitic diseases of animals. In: Veterinary Diagnostics: Current Trends. Gupta, R.P., Garg, S.R., Nehra, V. and Lather, D. (eds.), Satish Serial Publishing House, Delhi, pp. 81-112.
  8. Nagamani, K., Pavuluri, P.R.R., Gyaneshwari, M., Prasanthi, K., Rao, M.I.S. and Saxena, N.K. (2007). Molecular characterization of Cryptosporidium: an emerging parasite. Indian J. Med. Microbiol., 25(2): 133-136.
  9. Randhawa, S.S., Zahid, U.N., Randhawa, Swaran S, Juyal, P.D., Singla, L.D.and Uppal, S.K. (2012). Therapeutic management of cryptosporidiosis in cross bred dairy calves. Indian Vet. J. 89: 17-19.
  10. Singla, L.D., Gupta, M.P., Singh, H., Singh, S.T., Kaur, P. and Juyal, P.D. (2013). Antigen based diagnosis of Cryptosporidium parvum infection in faeces of cattle and buffalo calves. Indian J. Anim. Sci. 83(1): 37-39.
  11. Slavin, D. (1955). Cryptosporidium meleagridis (sp.nov.). J. Comp. Pathol. 65: 262-266.
  12. Sood, N.K., Singla, L.D., Brar, A.P.S. and Gupta, K. (2012). Cryptosporidiosis: An emerging zoonotic disease. Proceedings of International Symposium on One Health: Way Forward to Challenges in Food Safety and Zoonoses in 21st Century and XIth National Conference of Indian Association of Veterinary Public Health Specialists held from December 13-14, 2012 at GADVASU, Ludhiana, pp. 78-84.
  13. Sponseller, J.K., Griffiths, J.K. and Tzipori, S (2014). The evolution of respiratory cryptosporidiosis: evidence for transmission by inhalation. Clin. Microbiol. Rev. 27: 575–86.
  14. Syakalima, M., Noinyane, M.I., Ramaili, T., Morsei, L. and Nyirenda, M. (2015). A coprological assessment of cryptosporidiosis and giardiasis in pigs of Mafikeng villages, north west province of South Africa. Indian J. Anim. Res. 49(1): 132-135. DOI: 10.5958/0976-0555.2015.00027.8 
  15. Thakre, B.J., Solanki, J.B., Kumar, N. and Vargese, A. (2017). Comparative evaluation of conventional staining method and enzyme linked immunosorbent assay kits for the detection of bovine cryptosporidiosis. Indian J. Anim. Res. DOI: https://doi.org/    10.18805/ijar.v0iOF.9120
  16. Tyzzer, E.E. (1907). A sporozoan found in the peptic glands of the common mouse. Proc. Soc. Exp. Biol. Med. 5: 12–13.
  17. Venu, R., Latha, B.R., Abdul Basith, S., Sreekumar, C., Dhinakar Raj. G. and Raman, M. (2013). Factors influencing on prevalence of Cryptosporidium infection in south Indian dairy calves. J. Parasit. Dis. 37(2): 168-172.
  18. Widmer, G. (1998). Genetic heterogeneity and PCR detection of Cryptosporidium parvum. Adv. Parasitol. 40: 223-39. 
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    Research Article

    volume 53 issue 2 (february 2019) : 275-277,   Doi: 10.18805/ijar.B-3487

    Cryptosporidium oocyst shedding in buffalo calves in Haryana: A case study

    K
    Krutanjali Swain
    A
    Abhilash Routray
    S
    Saraswat Sahoo
    S
    Subha Ganguly
    1Department of Veterinary Parasitology, Faculty of Veterinary Science and Animal Husbandry, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar-125004, Haryana, India.
    Cite article:- Swain Krutanjali, Routray Abhilash, Sahoo Saraswat, Ganguly Subha (2018). Cryptosporidium oocyst shedding in buffalo calves in Haryana: A case study. Indian Journal of Animal Research. 53(2): 275-277. doi: 10.18805/ijar.B-3487.

    ABSTRACT

    Bovine cryptosporidiosisis primarily associated with neonatal diarrhoea with higher morbidity than mortality in young calves till they attain immunological maturity. The present investigation relates to a report on the shedding of Cryptosporidium oocyst in two buffalo calves of buffalo farm, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar from 1st days up to 3 month of age at 15 days interval using simple conventional microscopy. By using formol-ether concentration technique followed by modified Ziehl-Neelsen (ZN) acid fast staining, Cryptosporidium oocysts were concentrated and identified. The Cryptosporidium oocysts appeared as reddish pink coloured bodies against a bluish/greenish coloured background at oil immersion using ZN staining kit. The maximum oocyst shedding was observed (2.3 oocyst / field) during 16 to 30 days of age. The oocyst shedding gradually decreased with increase in age afterwards clearly indicating the disease of young buffalo calves.

    REFERENCES

    1. Bhagat, M., Sood, S., Yadav, A., Katoch, R., Chakraborty, D., Godara, R., Sultana, M. and Sangha, N. (2017). Clinico-haematological studies on experimental Cryptosporidium parvum Jammu isolate infection in Swiss albino mice. Indian J. Anim. Res. 51(5): 1-6. DOI: 10.18805/ijar.B-3318
    2. Bhat, S.A., Juyal, P.D. and Singla, L.D. (2012). Prevalence of cryptosporidiosis in neonatal buffalo calves in Ludhiana district of Punjab, India. Asian J. Anim. Vet. Adv. 7(6): 512-520.
    3. Bhat, S.A., Juyal, P.D. and Singla, L.D. (2013). Bovine cryptosporidiosis: brief review of its distribution in India. Trends Parasitol. Res. 2(2): 5-13.
    4. Desai, N.T., Sarkar, R. And Kang, G. (2012). Cryptosporidiosis: an under-recognized public health problem. Trop. Parasitol. 2(2): 91-98.
    5. Fayer, R., Morgan, U. and, Upton, S.J. (2000). Epidemiology of Cryptosporidium: transmission, detection and identification. Int. J. Parasitol. 30: 1305–1322.
    6. Fayer, R. and Xiao, L. (2008). Cryptosporidium and cryptosporidiosis, 2nd ed. CRC press, Boca Raton, UK. pp. 560.
    7. Gupta, S.K. and Singla, L.D. (2012). Diagnostic trends in parasitic diseases of animals. In: Veterinary Diagnostics: Current Trends. Gupta, R.P., Garg, S.R., Nehra, V. and Lather, D. (eds.), Satish Serial Publishing House, Delhi, pp. 81-112.
    8. Nagamani, K., Pavuluri, P.R.R., Gyaneshwari, M., Prasanthi, K., Rao, M.I.S. and Saxena, N.K. (2007). Molecular characterization of Cryptosporidium: an emerging parasite. Indian J. Med. Microbiol., 25(2): 133-136.
    9. Randhawa, S.S., Zahid, U.N., Randhawa, Swaran S, Juyal, P.D., Singla, L.D.and Uppal, S.K. (2012). Therapeutic management of cryptosporidiosis in cross bred dairy calves. Indian Vet. J. 89: 17-19.
    10. Singla, L.D., Gupta, M.P., Singh, H., Singh, S.T., Kaur, P. and Juyal, P.D. (2013). Antigen based diagnosis of Cryptosporidium parvum infection in faeces of cattle and buffalo calves. Indian J. Anim. Sci. 83(1): 37-39.
    11. Slavin, D. (1955). Cryptosporidium meleagridis (sp.nov.). J. Comp. Pathol. 65: 262-266.
    12. Sood, N.K., Singla, L.D., Brar, A.P.S. and Gupta, K. (2012). Cryptosporidiosis: An emerging zoonotic disease. Proceedings of International Symposium on One Health: Way Forward to Challenges in Food Safety and Zoonoses in 21st Century and XIth National Conference of Indian Association of Veterinary Public Health Specialists held from December 13-14, 2012 at GADVASU, Ludhiana, pp. 78-84.
    13. Sponseller, J.K., Griffiths, J.K. and Tzipori, S (2014). The evolution of respiratory cryptosporidiosis: evidence for transmission by inhalation. Clin. Microbiol. Rev. 27: 575–86.
    14. Syakalima, M., Noinyane, M.I., Ramaili, T., Morsei, L. and Nyirenda, M. (2015). A coprological assessment of cryptosporidiosis and giardiasis in pigs of Mafikeng villages, north west province of South Africa. Indian J. Anim. Res. 49(1): 132-135. DOI: 10.5958/0976-0555.2015.00027.8 
    15. Thakre, B.J., Solanki, J.B., Kumar, N. and Vargese, A. (2017). Comparative evaluation of conventional staining method and enzyme linked immunosorbent assay kits for the detection of bovine cryptosporidiosis. Indian J. Anim. Res. DOI: https://doi.org/    10.18805/ijar.v0iOF.9120
    16. Tyzzer, E.E. (1907). A sporozoan found in the peptic glands of the common mouse. Proc. Soc. Exp. Biol. Med. 5: 12–13.
    17. Venu, R., Latha, B.R., Abdul Basith, S., Sreekumar, C., Dhinakar Raj. G. and Raman, M. (2013). Factors influencing on prevalence of Cryptosporidium infection in south Indian dairy calves. J. Parasit. Dis. 37(2): 168-172.
    18. Widmer, G. (1998). Genetic heterogeneity and PCR detection of Cryptosporidium parvum. Adv. Parasitol. 40: 223-39. 
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