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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 2 (february 2019) : 200-203,   Doi: 10.18805/ijar.B-3483

Identification of point mutation in Exon 2 of MBL1 gene and its relationship with Somatic Cell Score (SCS) in Sahiwal cattle

K
Kamaldeep
A
Ankit Magotra
B
B.L.Pander
M
Manjeet
B
B.S. Malik
D
D.S. Dalal
1Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar-125 001, Haryana, India.
Cite article:- Kamaldeep, Magotra Ankit, B.L.Pander, Manjeet, Malik B.S., Dalal D.S. (2017). Identification of point mutation in Exon 2 of MBL1 gene and its relationship with Somatic Cell Score (SCS) in Sahiwal cattle. Indian Journal of Animal Research. 53(2): 200-203. doi: 10.18805/ijar.B-3483.

ABSTRACT

Mannose-binding lectin (MBL) is one of the most important constituent and a pattern recognition molecule of the innate immune system. MBL gene possibly contributes to bacterial infection resistance and was proposed as a molecular marker for milk production traits to control mastitis.  Due to its location near to QTLs associated with Somatic cell score (SCS) and mastitis , the MBL1 gene may be considered as a candidate gene. Sahiwal cows in milk were selected randomly to identify g.2686T>C point mutation in exon 2 of MBL1 gene and its association with somatic cell score. A 401 bp PCR fragment of MBL1 gene encompassing the exon 2 was amplified and digested with Hae III to screen the reported SNP. Genotype analysis using PCR-RFLP revealed a polymorphic banding pattern. Sequencing was also carried out to validate the PCR-RFLP results of nucleotide sequence in a particular region. The preliminary information obtained from the study indicated that the targeted variantof MBL1 gene in indigenous cattle had no association with SCS and mastitis resistance which may be a breed specific characteristic. Since present study has formulated the results based on a relatively small sample size, further studies with large sample size are required to validate these results.

REFERENCES

  1. Bouwman, L.H., Roep, B.O. and Roos, A. (2006) Mannose-binding lectin: clinical implications for infection, transplantation, and autoimmunity. Hum Immunol 67:247–256.
  2. Burman, P.(2002) Mastitis: Expert calls for early detection. http://www.thehindubusinessline.in/2002/09/10/stories/2002091000201300. htm.Accessed on 09-06-2014.
  3. Gjerstorff, M., Hansen, S., Jensen, B., Dueholm, B., Horn, P., Bendixen, C. and Holmskov, U. (2004). The genes encoding bovine SP-    A, SP-D, MBL-A, conglutinin, CL-43 and CL-46 form a distinct collectin locus on Bos taurus chromosome 28 (BTA28) at position q.1.8–1.9. Anim. Genet. 35, 333–337.
  4. Henna. H., Azmat, A. K., Tufail, M. B., Ifat, A., Nida, H., Asma, B. S., Ambreen, H. (2013). Bovine Mastitis - A disease of serious concern for dairy farmers. International Journal of Livestock Research. 3:42-55.
  5. Heringstad, B., Klemetsdal, G. and Steine, T. (2003). Selection Responses for Clinical Mastitis and Protein Yield in Two Norwegian Dairy Cattle Selection Experiments.Journal of Dairy Science; 86( 9) : 2990–2999
  6. Hou. G.Y., Yuan, Z.R., Gao, X., Li, J.Y., Gao, H.J., Chen, J.B., Xu, S.Z., (2010). Genetic polymorphisms of the CACNA2D1 gene and their association with carcass and meat quality traits in cattle. Biochemical Genetics. 48:751-759.
  7. Liu, J., Ju, Z., Li, Q., Huang, J., Li, R., Li, J., Ma, L., Zhong, J. and Wang, C. (2011). Mannose-binding lectin 1 haplotypes influence serum MBL-A concentration, complement activity, and milk production traits in Chinese Holstein cattle. Immunogenetics. 63: 727–742.
  8. Magotra, A., Gupta, I.D., Verma, A., Rani, A., Arya, A., Vineeth, M.R., and Kumar, V. (2016). Characterization and validation of point mutation in Exon 19 of Calcium Channel, voltage-dependent, Alpha-2/Delta subunit 1(CACNA2D1) gene and its relationship with mastitis traits in Sahiwal. Indian J. Anim. Res., DOI:10.18805/ijar.10990. 
  9. Neth, O., Jack, D.L., Dodds, A.W., Holzel, H., Klein, N.J. and Turner, M.W. (2000). Mannose-binding lectin binds to a range of clinically relevant microorganisms and promotes complement deposition. Infect Immun 68:688–693
  10. Rupp, R. and Boichard, D. (2003). Genetics of resistance to mastitis in dairy cattle. Vet. Res. 34: 671–688.
  11. Sambrook, J. and Russell, D. W. (1989). Preparation and analysis of eukaryotic DNA. In: Molecular Cloning: A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press, New York: 6.1-6.62.
  12. Wang, C.F., Liu, M., Li, Q.L., Ju, Z.H., Huang, J.M., Li, J.B. and Zhong, J.F. ( 2011).Three novel single-nucleotide polymorphims of MBL1 gene in Chinese native cattle and their associations with milk performance traits. Vet. Immunol. Immunopathol. 139, 229–236.
  13. Yuan, Z., Li, J., Li, J., Gao, X. and Xu, S. (2013). SNPs identification and its correlation analysis with milk somatic cell score in bovine MBL1 gene. Mol. Biol. Rep.,40 (1): 7-12.
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    Research Article

    volume 53 issue 2 (february 2019) : 200-203,   Doi: 10.18805/ijar.B-3483

    Identification of point mutation in Exon 2 of MBL1 gene and its relationship with Somatic Cell Score (SCS) in Sahiwal cattle

    K
    Kamaldeep
    A
    Ankit Magotra
    B
    B.L.Pander
    M
    Manjeet
    B
    B.S. Malik
    D
    D.S. Dalal
    1Department of Animal Genetics and Breeding, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar-125 001, Haryana, India.
    Cite article:- Kamaldeep, Magotra Ankit, B.L.Pander, Manjeet, Malik B.S., Dalal D.S. (2017). Identification of point mutation in Exon 2 of MBL1 gene and its relationship with Somatic Cell Score (SCS) in Sahiwal cattle. Indian Journal of Animal Research. 53(2): 200-203. doi: 10.18805/ijar.B-3483.

    ABSTRACT

    Mannose-binding lectin (MBL) is one of the most important constituent and a pattern recognition molecule of the innate immune system. MBL gene possibly contributes to bacterial infection resistance and was proposed as a molecular marker for milk production traits to control mastitis.  Due to its location near to QTLs associated with Somatic cell score (SCS) and mastitis , the MBL1 gene may be considered as a candidate gene. Sahiwal cows in milk were selected randomly to identify g.2686T>C point mutation in exon 2 of MBL1 gene and its association with somatic cell score. A 401 bp PCR fragment of MBL1 gene encompassing the exon 2 was amplified and digested with Hae III to screen the reported SNP. Genotype analysis using PCR-RFLP revealed a polymorphic banding pattern. Sequencing was also carried out to validate the PCR-RFLP results of nucleotide sequence in a particular region. The preliminary information obtained from the study indicated that the targeted variantof MBL1 gene in indigenous cattle had no association with SCS and mastitis resistance which may be a breed specific characteristic. Since present study has formulated the results based on a relatively small sample size, further studies with large sample size are required to validate these results.

    REFERENCES

    1. Bouwman, L.H., Roep, B.O. and Roos, A. (2006) Mannose-binding lectin: clinical implications for infection, transplantation, and autoimmunity. Hum Immunol 67:247–256.
    2. Burman, P.(2002) Mastitis: Expert calls for early detection. http://www.thehindubusinessline.in/2002/09/10/stories/2002091000201300. htm.Accessed on 09-06-2014.
    3. Gjerstorff, M., Hansen, S., Jensen, B., Dueholm, B., Horn, P., Bendixen, C. and Holmskov, U. (2004). The genes encoding bovine SP-    A, SP-D, MBL-A, conglutinin, CL-43 and CL-46 form a distinct collectin locus on Bos taurus chromosome 28 (BTA28) at position q.1.8–1.9. Anim. Genet. 35, 333–337.
    4. Henna. H., Azmat, A. K., Tufail, M. B., Ifat, A., Nida, H., Asma, B. S., Ambreen, H. (2013). Bovine Mastitis - A disease of serious concern for dairy farmers. International Journal of Livestock Research. 3:42-55.
    5. Heringstad, B., Klemetsdal, G. and Steine, T. (2003). Selection Responses for Clinical Mastitis and Protein Yield in Two Norwegian Dairy Cattle Selection Experiments.Journal of Dairy Science; 86( 9) : 2990–2999
    6. Hou. G.Y., Yuan, Z.R., Gao, X., Li, J.Y., Gao, H.J., Chen, J.B., Xu, S.Z., (2010). Genetic polymorphisms of the CACNA2D1 gene and their association with carcass and meat quality traits in cattle. Biochemical Genetics. 48:751-759.
    7. Liu, J., Ju, Z., Li, Q., Huang, J., Li, R., Li, J., Ma, L., Zhong, J. and Wang, C. (2011). Mannose-binding lectin 1 haplotypes influence serum MBL-A concentration, complement activity, and milk production traits in Chinese Holstein cattle. Immunogenetics. 63: 727–742.
    8. Magotra, A., Gupta, I.D., Verma, A., Rani, A., Arya, A., Vineeth, M.R., and Kumar, V. (2016). Characterization and validation of point mutation in Exon 19 of Calcium Channel, voltage-dependent, Alpha-2/Delta subunit 1(CACNA2D1) gene and its relationship with mastitis traits in Sahiwal. Indian J. Anim. Res., DOI:10.18805/ijar.10990. 
    9. Neth, O., Jack, D.L., Dodds, A.W., Holzel, H., Klein, N.J. and Turner, M.W. (2000). Mannose-binding lectin binds to a range of clinically relevant microorganisms and promotes complement deposition. Infect Immun 68:688–693
    10. Rupp, R. and Boichard, D. (2003). Genetics of resistance to mastitis in dairy cattle. Vet. Res. 34: 671–688.
    11. Sambrook, J. and Russell, D. W. (1989). Preparation and analysis of eukaryotic DNA. In: Molecular Cloning: A Laboratory Manual. 3rd Edition. Cold Spring Harbor Laboratory Press, New York: 6.1-6.62.
    12. Wang, C.F., Liu, M., Li, Q.L., Ju, Z.H., Huang, J.M., Li, J.B. and Zhong, J.F. ( 2011).Three novel single-nucleotide polymorphims of MBL1 gene in Chinese native cattle and their associations with milk performance traits. Vet. Immunol. Immunopathol. 139, 229–236.
    13. Yuan, Z., Li, J., Li, J., Gao, X. and Xu, S. (2013). SNPs identification and its correlation analysis with milk somatic cell score in bovine MBL1 gene. Mol. Biol. Rep.,40 (1): 7-12.
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