Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 1 (january 2019) : 41-44,   Doi: 10.18805/ijar.B-3464

Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India

G
G. Kadirvel
M
M. K. Kalita
R
Raju Kr. Dewry
A
Ashok Kumar
N
Nripendra Mahanta
L
L. Anandakumar Singh
S
S.K. Baishya
S
S.V. Ngachan
1Division of Animal Production, ICAR- Research Complex for NEH Region, Umiam-793 103, Meghalaya, India.
Cite article:- Kadirvel G., Kalita K. M., Dewry Kr. Raju, Kumar Ashok, Mahanta Nripendra, Singh Anandakumar L., Baishya S.K., Ngachan S.V. (2018). Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India. Indian Journal of Animal Research. 53(1): 41-44. doi: 10.18805/ijar.B-3464.

ABSTRACT

Study was conducted to compare the semen quality and fertility of liquid stored semen for three days and frozen-thawed semen in the north-eastern region of India. For liquid semen, the semen ejaculates were extended in Beltsville Thawing Solution (BTS) extender and preserved at 17°C for three days. For cryopreservation, semen was diluted Lactose-egg yolk-glycerol extender and frozen in straw using programmable freezer with freezing rate of 40°C/min from -6 to -140°C. The preserved evaluated for sperm motility, viability, plasma membrane integrity and fertility. The results revealed that the liquid stored semen has maintained the sperm motility and viability up to day 3 without significant reduction. Similarly the plasma membrane integrity did not differ significantly up to day 2, but it was significantly (P<0.05) reduced on days 3 in liquid stored semen. After freezing and thawing, the mean sperm motility, viability and plasma membrane integrity were 58.25 ± 2.96%, 64.75 ± 2.47% and 47.06 ± 2.02%, respectively. These parameters were significantly (P<0.01) lower as compared to the liquid stored semen from day 0 to day 3. After insemination with liquid semen, the farrowing rate was 77.7%, 80.76%, 73.07% and 69.8%, respectively from day 0, day1, day 2 and day 3. The pregnancy rate, farrowing rate and litter size did not differ significantly among different days of liquid storage. These parameters were significantly (P<0.01) lower in frozen semen as compare to that of liquid stored semen. The study concluded that the liquid semen stored up to three days is more efficient than frozen-thawed semen in terms of preserving sperm quality and fertility.

REFERENCES

  1. Apiæ J,Vakanjac S, Stanèiæ I, Radoviæ I, Jotanoviæ S, Kanaèki Z, Stankoviæ B. (2015). Sow fertility after insemination with varying doses of volume and spermatozoa count.Turk J Vet Anim Sci 39:709-713
  2. Bailey J L, Lessard C, Jacques J, Breque C, Dobrinski I, Zeng W and Galantino-Homer, H L. (2008). Cryopreservation of boar semen and its future importance to the industry. Theriogenology 70:1251-1259.
  3. Baishya S K, Biswas R K, Kadirvel G, Deka B C, Suresh Kumar and Ngachan S V. (2015). First report on in vivo fertility trial of frozen thawed boar semen in India. Indian J. Anim. Res., 50 (2) 2016:181-184
  4. Campbell, R G, Hancock J L and Rothschild L. (1953).Counting live and dead bull spermatozoa.J. Exp. Biol.30:44-49.
  5. Corcuera B D, Marigorta P, Sagüés A, Saiz-Cidoncha F and Pérez-Gutiérrez J F. (2007). Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa.Theriogenology 67:1150-    1157.
  6. De Ambrogi M., Ballester J., Saravia F., Caballero I., Johannisson A., Wallgren M., Andersson, M. and Rodriguez-Martinez H. (2006). Effect of storage in short- and long-term commercial semen extenders on the motility, plasma membrane and chromatin integrity of boar spermatozoa.Int. J. Androl. 29:543-552.
  7. Didion B A, Braun G and Duggan M. (2011). Field fertility of frozen boar sperm: a retrospective report spanning 2007 to 2010 comprising over 2000 AI services. Reprod. Dom. Anim. 46: 84-110.
  8. Frydrychová S, Èeøovský J, Lustyková A and Rozkot M. (2010). Effects of long term liquid commercial semen extender and storage time on the membrane quality of boar semen. Czech.J. Anim. Sci. 55:160-166.
  9. Gaczarzewicz, D, Udala, J, Piasecka M, Blaszczyk B and Stankiewicz T. (2015). Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability.Turk J Biol. 39:582-594.
  10. Garcia, J C, Abad M and Kirkwood R N. (2007). Effect of sperm numbers and time of insemination relative to ovulation on sow fertility.Anim. Reprod. Sci. 100:397-401.
  11. Johnson L A, Aalbers J G and Grooten H J G. (1988). Artificial insemination of swine: Fecundity of boar semen stored in Beltsville TS BTS, Modified Modena MM, or MR-A and inseminated on one, three and four days after collection. Zuchthygiene 23:49-55.
  12. Johnson L A, Weitze K F, Fiser P and Maxwell W M C. (2000). Storage of boar semen. Anim. Reprod. Sci. 62:143-172.
  13. Kadirvel G, Kumaresan A, Das A, Bujarbaruah K. M, Venkatasubramanian V and Ngachan S. V. (2013). Artificial insemination of pigs reared under smallholder production system in northeastern India: Success rate, genetic improvement and monitory benefit. Trop. Anim. Health Prod. 45: 679-686.
  14. Kadirvel G, Perumal P, Sarath T, Jerome A, Syamal N, Das A, Hasin D and Baishya S K. (2016). Efficacy of different extenders on sperm characteristics and fertility in crossbred pigs of north-eastern India. VeterinarskiArhiv 86:515-528.
  15. Kaeoket K, Srisowanna T, Wichaidit U, Chanapiwat P, Maneein S. (2010). Comparative study on six different long term commercial extenders for fresh boar semen. Thai J Vet Med 40:257-263.
  16. Khalifa T, Rekkas C, Samartzi F, Lymberopoulos A, Kousenidis K and Dovenski T. (2014). Highlights on artificial insemination (AI) technology in the pigs. Macedonian Veterinary Review.37:5-34
  17. Mapeka M H, Lehloenya K C, and Nedambale T L. (2012). Comparison of different extenders and storage temperature on the sperm motility characteristics of Kolbroek pig semen. S. Afr. J. Anim. Sci. 42 (Suppl 1):530-533.
  18. Revell S G and Mrode R A. (1994). An osmotic resistance test for bovine semen. Anim. Reprod. Sci., 36:77-86.
  19. Snedecor G W and Cochran W G. (1994). Statistical Methods (eighth edition). Oxford & IBH Publishing Co. Calcutta, India
  20. Visalvethaya W, Tantasuparuk W and Techakumphu M. (2010). The development of a model for artificial insemination by backyard pig farmers in Thailand.Trop. Anim. Health Prod. 43:787-793.
  21. Westendorf P, Richter L and Treu H. (1975). ZurTiefgefrierung von Ebersperma. Labor-und Besamungsergebnissemitdem Hiilsenberger Pailletten-verfahren. Dtsch Tierarztl Wschr 82:261-267.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 53 issue 1 (january 2019) : 41-44,   Doi: 10.18805/ijar.B-3464

    Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India

    G
    G. Kadirvel
    M
    M. K. Kalita
    R
    Raju Kr. Dewry
    A
    Ashok Kumar
    N
    Nripendra Mahanta
    L
    L. Anandakumar Singh
    S
    S.K. Baishya
    S
    S.V. Ngachan
    1Division of Animal Production, ICAR- Research Complex for NEH Region, Umiam-793 103, Meghalaya, India.
    Cite article:- Kadirvel G., Kalita K. M., Dewry Kr. Raju, Kumar Ashok, Mahanta Nripendra, Singh Anandakumar L., Baishya S.K., Ngachan S.V. (2018). Semen quality and fertility of liquid stored and frozen-thawed semen in crossbred pigs of North-Eastern India. Indian Journal of Animal Research. 53(1): 41-44. doi: 10.18805/ijar.B-3464.

    ABSTRACT

    Study was conducted to compare the semen quality and fertility of liquid stored semen for three days and frozen-thawed semen in the north-eastern region of India. For liquid semen, the semen ejaculates were extended in Beltsville Thawing Solution (BTS) extender and preserved at 17°C for three days. For cryopreservation, semen was diluted Lactose-egg yolk-glycerol extender and frozen in straw using programmable freezer with freezing rate of 40°C/min from -6 to -140°C. The preserved evaluated for sperm motility, viability, plasma membrane integrity and fertility. The results revealed that the liquid stored semen has maintained the sperm motility and viability up to day 3 without significant reduction. Similarly the plasma membrane integrity did not differ significantly up to day 2, but it was significantly (P<0.05) reduced on days 3 in liquid stored semen. After freezing and thawing, the mean sperm motility, viability and plasma membrane integrity were 58.25 ± 2.96%, 64.75 ± 2.47% and 47.06 ± 2.02%, respectively. These parameters were significantly (P<0.01) lower as compared to the liquid stored semen from day 0 to day 3. After insemination with liquid semen, the farrowing rate was 77.7%, 80.76%, 73.07% and 69.8%, respectively from day 0, day1, day 2 and day 3. The pregnancy rate, farrowing rate and litter size did not differ significantly among different days of liquid storage. These parameters were significantly (P<0.01) lower in frozen semen as compare to that of liquid stored semen. The study concluded that the liquid semen stored up to three days is more efficient than frozen-thawed semen in terms of preserving sperm quality and fertility.

    REFERENCES

    1. Apiæ J,Vakanjac S, Stanèiæ I, Radoviæ I, Jotanoviæ S, Kanaèki Z, Stankoviæ B. (2015). Sow fertility after insemination with varying doses of volume and spermatozoa count.Turk J Vet Anim Sci 39:709-713
    2. Bailey J L, Lessard C, Jacques J, Breque C, Dobrinski I, Zeng W and Galantino-Homer, H L. (2008). Cryopreservation of boar semen and its future importance to the industry. Theriogenology 70:1251-1259.
    3. Baishya S K, Biswas R K, Kadirvel G, Deka B C, Suresh Kumar and Ngachan S V. (2015). First report on in vivo fertility trial of frozen thawed boar semen in India. Indian J. Anim. Res., 50 (2) 2016:181-184
    4. Campbell, R G, Hancock J L and Rothschild L. (1953).Counting live and dead bull spermatozoa.J. Exp. Biol.30:44-49.
    5. Corcuera B D, Marigorta P, Sagüés A, Saiz-Cidoncha F and Pérez-Gutiérrez J F. (2007). Effect of lactose and glycerol on the motility, normal apical ridge, chromatin condensation and chromatin stability of frozen boar spermatozoa.Theriogenology 67:1150-    1157.
    6. De Ambrogi M., Ballester J., Saravia F., Caballero I., Johannisson A., Wallgren M., Andersson, M. and Rodriguez-Martinez H. (2006). Effect of storage in short- and long-term commercial semen extenders on the motility, plasma membrane and chromatin integrity of boar spermatozoa.Int. J. Androl. 29:543-552.
    7. Didion B A, Braun G and Duggan M. (2011). Field fertility of frozen boar sperm: a retrospective report spanning 2007 to 2010 comprising over 2000 AI services. Reprod. Dom. Anim. 46: 84-110.
    8. Frydrychová S, Èeøovský J, Lustyková A and Rozkot M. (2010). Effects of long term liquid commercial semen extender and storage time on the membrane quality of boar semen. Czech.J. Anim. Sci. 55:160-166.
    9. Gaczarzewicz, D, Udala, J, Piasecka M, Blaszczyk B and Stankiewicz T. (2015). Storage temperature of boar semen and its relationship to changes in sperm plasma membrane integrity, mitochondrial membrane potential, and oxidoreductive capability.Turk J Biol. 39:582-594.
    10. Garcia, J C, Abad M and Kirkwood R N. (2007). Effect of sperm numbers and time of insemination relative to ovulation on sow fertility.Anim. Reprod. Sci. 100:397-401.
    11. Johnson L A, Aalbers J G and Grooten H J G. (1988). Artificial insemination of swine: Fecundity of boar semen stored in Beltsville TS BTS, Modified Modena MM, or MR-A and inseminated on one, three and four days after collection. Zuchthygiene 23:49-55.
    12. Johnson L A, Weitze K F, Fiser P and Maxwell W M C. (2000). Storage of boar semen. Anim. Reprod. Sci. 62:143-172.
    13. Kadirvel G, Kumaresan A, Das A, Bujarbaruah K. M, Venkatasubramanian V and Ngachan S. V. (2013). Artificial insemination of pigs reared under smallholder production system in northeastern India: Success rate, genetic improvement and monitory benefit. Trop. Anim. Health Prod. 45: 679-686.
    14. Kadirvel G, Perumal P, Sarath T, Jerome A, Syamal N, Das A, Hasin D and Baishya S K. (2016). Efficacy of different extenders on sperm characteristics and fertility in crossbred pigs of north-eastern India. VeterinarskiArhiv 86:515-528.
    15. Kaeoket K, Srisowanna T, Wichaidit U, Chanapiwat P, Maneein S. (2010). Comparative study on six different long term commercial extenders for fresh boar semen. Thai J Vet Med 40:257-263.
    16. Khalifa T, Rekkas C, Samartzi F, Lymberopoulos A, Kousenidis K and Dovenski T. (2014). Highlights on artificial insemination (AI) technology in the pigs. Macedonian Veterinary Review.37:5-34
    17. Mapeka M H, Lehloenya K C, and Nedambale T L. (2012). Comparison of different extenders and storage temperature on the sperm motility characteristics of Kolbroek pig semen. S. Afr. J. Anim. Sci. 42 (Suppl 1):530-533.
    18. Revell S G and Mrode R A. (1994). An osmotic resistance test for bovine semen. Anim. Reprod. Sci., 36:77-86.
    19. Snedecor G W and Cochran W G. (1994). Statistical Methods (eighth edition). Oxford & IBH Publishing Co. Calcutta, India
    20. Visalvethaya W, Tantasuparuk W and Techakumphu M. (2010). The development of a model for artificial insemination by backyard pig farmers in Thailand.Trop. Anim. Health Prod. 43:787-793.
    21. Westendorf P, Richter L and Treu H. (1975). ZurTiefgefrierung von Ebersperma. Labor-und Besamungsergebnissemitdem Hiilsenberger Pailletten-verfahren. Dtsch Tierarztl Wschr 82:261-267.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)