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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 1 (january 2019) : 19-23,   Doi: 10.18805/ijar.B-3458

Genetic characterization of local goats of Karnataka by microsatellite marker analysis*

R
R. Jayashree
M
M.R. Jayashankar
C
C.S. Nagaraja
I
Isloor Shrikrishna
K
K. Satyanarayana
1Department of Animal Genetics and Breeding, Veterinary College, Hebbal-560 024, Bangalore, Karnataka, India.
Cite article:- Jayashree R., Jayashankar M.R., Nagaraja C.S., Shrikrishna Isloor, Satyanarayana K. (2018). Genetic characterization of local goats of Karnataka by microsatellite marker analysis*. Indian Journal of Animal Research. 53(1): 19-23. doi: 10.18805/ijar.B-3458.

ABSTRACT

The diversity status of local goats of Karnataka was studied by using microsatellite marker analysis. The genomic DNA from unrelated local goats were PCR- amplified with a panel of 23 microsatellite markers. Microsatellite PCR products were multiplexed and run on capillary based genetic analyser and the raw data obtained was analysed. Totally 158 alleles were observed and the number of alleles ranged from three (ILSTS005 and OarJMP29) to 13 (RM088). The number of effective alleles ranged from 2.25 (ILSTS005) to 8.40 (RM088) in all the 23 loci studied. The mean observed heterozygosity (Ho) was 0.4698±0.2214 [range 0 (ETH225) to 0.8462 (ILSTS034)] and the mean expected heterozygosity (He) was 0.7471± 0.1098 [range 0.5656 (ILSTS005) to 0.9138 (SRCRSP 8)] indicating the heterogenous nature of the local goat population of Karnataka. 

REFERENCES

  1. Anonymous(2014). Government of India GOI. Basic Animal Husbandry Statistics Ministry of Agricluture, Department of Animal Husbandry, Dairying and Fisheries. New Delhi.
  2. Dixit, S.P. Verma. N.K., Alawati, S.P.S., Aggarwal, R.A.K. Kumar, S. and Singh, K.P., 2008b. Molecular genetic characterization of Kutchi breed of goat. Curr. Sci., 95(7), 946-952.
  3. Dixit. S. P.,Verma, N. K., Aggarwal, R. A. K, Vyas, M. K., Rana, J. Sharma, A. (2010), Genetic diversity and relationship among southern Indian Goat Breeds based on Microsatellite markers. Small Rumin. Res., 91:153–159.
  4. Fatima, S., Bhong, C. D., Rank, D. N. and Joshi, C. G.(2008). Genetic variability and bottleneck studies in Zalawadi, Gohilwadi and Surti goat breeds of Gujarat (India) using microsatellites. Small Ruminant Research, 77: 58-64. 
  5. Islam, M.M , Shabana Anjum, R. J. Modi and K. N. Wadhwani.(2016). International Journal of Science Environment and Technology 5(3): 956-965..
  6. Kranti Kharkar, S.V. Kuralkar* and Prajakta Kuralkar. (2015). Molecular genetic characterization of Berari breed of goat using microsatellite markers. Indian J. Anim. Res., 49 (4) 423-428
  7. Kumar, D., Dixit, S. P., Sharma, R., Pandey, A. K., Sirohi, G., Patel, A. K., Aggarwal, R., Verma, N. K., Gour, D. and Ahlawat, S., 2005a. Population structure genetic variation and management of Marwari goats. Small Ruminant Research, 59: 41-48. 
  8. Litt, M. and Lutty, J. A. (1989). A hyper variable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am. J. Hum. Genet., 44: 397-401.
  9. Luikart, G., Biju0DuvalL, M. P., Ertugrul, O., Zagdsuren, Y., Maudet, C. and Taberlet, P., 1999. Power of 22 microsatellite markers in fluorescent multiplexes for parentage testing in goats (Capra hircus). Ani. Genet., 30: 431-438.
  10. Luty, J. A., Guo, Z., Willard, H. F., Ledbetter, D. H., Ledbetter, S. and Litt, M., 1990. Five polymorphic microsatellite VNTRs on the human X chromosome. Am. J. Hum. Genet., 46: 776-783.
  11. Machugh. D. E., Loftus, R. T., Cunningham, P., and Bradley, D. G.(1997). Microsatellite DNA variation and the evolution, domestication and phylogeography of Taurine and Zebu cattle (Bos taurus and Bos indicus). Genetics, 146: 1071–1086.
  12. Millers, S.A., Dykes, D. D. and. Polesky, H.F.(1998). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res, 16(3): 1215.
  13. Sharma, R., Pandey, A. K., Kumar, D., Alam, S. and Ahlawat, S. P. S.(2008). Genetic variability in Beetal goat breed assessed by microsatellite DNA profiling. Indian Journal of Animal Sciences, 78: 505-509.
  14. Singh G, Thakur Y, Kour A, Sankhyan V, Katoch S (2015) Genetic characterization of Gaddi goat breed of Western Himalayas using microsatellite markers, Veterinary World 8(4); 527-531.
  15. Yeh, F.C., Yang, R., Boyle, C., Timothy, B.J., Ye, Z.H. and Mao, J.X.(1999). POPGENE version 1.32, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre. University of Alberta, Canada. http://www. ualberta.ca/fyeh/
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    Research Article

    volume 53 issue 1 (january 2019) : 19-23,   Doi: 10.18805/ijar.B-3458

    Genetic characterization of local goats of Karnataka by microsatellite marker analysis*

    R
    R. Jayashree
    M
    M.R. Jayashankar
    C
    C.S. Nagaraja
    I
    Isloor Shrikrishna
    K
    K. Satyanarayana
    1Department of Animal Genetics and Breeding, Veterinary College, Hebbal-560 024, Bangalore, Karnataka, India.
    Cite article:- Jayashree R., Jayashankar M.R., Nagaraja C.S., Shrikrishna Isloor, Satyanarayana K. (2018). Genetic characterization of local goats of Karnataka by microsatellite marker analysis*. Indian Journal of Animal Research. 53(1): 19-23. doi: 10.18805/ijar.B-3458.

    ABSTRACT

    The diversity status of local goats of Karnataka was studied by using microsatellite marker analysis. The genomic DNA from unrelated local goats were PCR- amplified with a panel of 23 microsatellite markers. Microsatellite PCR products were multiplexed and run on capillary based genetic analyser and the raw data obtained was analysed. Totally 158 alleles were observed and the number of alleles ranged from three (ILSTS005 and OarJMP29) to 13 (RM088). The number of effective alleles ranged from 2.25 (ILSTS005) to 8.40 (RM088) in all the 23 loci studied. The mean observed heterozygosity (Ho) was 0.4698±0.2214 [range 0 (ETH225) to 0.8462 (ILSTS034)] and the mean expected heterozygosity (He) was 0.7471± 0.1098 [range 0.5656 (ILSTS005) to 0.9138 (SRCRSP 8)] indicating the heterogenous nature of the local goat population of Karnataka. 

    REFERENCES

    1. Anonymous(2014). Government of India GOI. Basic Animal Husbandry Statistics Ministry of Agricluture, Department of Animal Husbandry, Dairying and Fisheries. New Delhi.
    2. Dixit, S.P. Verma. N.K., Alawati, S.P.S., Aggarwal, R.A.K. Kumar, S. and Singh, K.P., 2008b. Molecular genetic characterization of Kutchi breed of goat. Curr. Sci., 95(7), 946-952.
    3. Dixit. S. P.,Verma, N. K., Aggarwal, R. A. K, Vyas, M. K., Rana, J. Sharma, A. (2010), Genetic diversity and relationship among southern Indian Goat Breeds based on Microsatellite markers. Small Rumin. Res., 91:153–159.
    4. Fatima, S., Bhong, C. D., Rank, D. N. and Joshi, C. G.(2008). Genetic variability and bottleneck studies in Zalawadi, Gohilwadi and Surti goat breeds of Gujarat (India) using microsatellites. Small Ruminant Research, 77: 58-64. 
    5. Islam, M.M , Shabana Anjum, R. J. Modi and K. N. Wadhwani.(2016). International Journal of Science Environment and Technology 5(3): 956-965..
    6. Kranti Kharkar, S.V. Kuralkar* and Prajakta Kuralkar. (2015). Molecular genetic characterization of Berari breed of goat using microsatellite markers. Indian J. Anim. Res., 49 (4) 423-428
    7. Kumar, D., Dixit, S. P., Sharma, R., Pandey, A. K., Sirohi, G., Patel, A. K., Aggarwal, R., Verma, N. K., Gour, D. and Ahlawat, S., 2005a. Population structure genetic variation and management of Marwari goats. Small Ruminant Research, 59: 41-48. 
    8. Litt, M. and Lutty, J. A. (1989). A hyper variable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am. J. Hum. Genet., 44: 397-401.
    9. Luikart, G., Biju0DuvalL, M. P., Ertugrul, O., Zagdsuren, Y., Maudet, C. and Taberlet, P., 1999. Power of 22 microsatellite markers in fluorescent multiplexes for parentage testing in goats (Capra hircus). Ani. Genet., 30: 431-438.
    10. Luty, J. A., Guo, Z., Willard, H. F., Ledbetter, D. H., Ledbetter, S. and Litt, M., 1990. Five polymorphic microsatellite VNTRs on the human X chromosome. Am. J. Hum. Genet., 46: 776-783.
    11. Machugh. D. E., Loftus, R. T., Cunningham, P., and Bradley, D. G.(1997). Microsatellite DNA variation and the evolution, domestication and phylogeography of Taurine and Zebu cattle (Bos taurus and Bos indicus). Genetics, 146: 1071–1086.
    12. Millers, S.A., Dykes, D. D. and. Polesky, H.F.(1998). A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res, 16(3): 1215.
    13. Sharma, R., Pandey, A. K., Kumar, D., Alam, S. and Ahlawat, S. P. S.(2008). Genetic variability in Beetal goat breed assessed by microsatellite DNA profiling. Indian Journal of Animal Sciences, 78: 505-509.
    14. Singh G, Thakur Y, Kour A, Sankhyan V, Katoch S (2015) Genetic characterization of Gaddi goat breed of Western Himalayas using microsatellite markers, Veterinary World 8(4); 527-531.
    15. Yeh, F.C., Yang, R., Boyle, C., Timothy, B.J., Ye, Z.H. and Mao, J.X.(1999). POPGENE version 1.32, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre. University of Alberta, Canada. http://www. ualberta.ca/fyeh/
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