Computer assisted semen analysis of deccani ram semen presevability at 5ºC

DOI: 10.18805/ijar.B-3450    | Article Id: B-3450 | Page : 1695-1700
Citation :- Computer assisted semen analysis of deccani ram semen presevability at 5ºC.Indian Journal Of Animal Research.2018.(52):1695-1700
M. Rajashri, K. Ramchandra Reddy, G. Aruna Kumari, N. Nalini Kumari and G. Srinivas rsri0835@gmail.com
Address : Department of Veterinary Gynaecology and Obstetrics, C.V.Sc, P.V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad-500 030, Telangana, India
Submitted Date : 9-06-2017
Accepted Date : 12-10-2017

Abstract

To investigate the preservability of Deccani ram semen in three different extenders - SC (Sodium Citrate), T (Tris), C (Coconut milk) egg yolk extenders after dilution and chilling (5ºC) at 1, 24 and 48 hr. of storage. Eight Deccani adult rams (aged 2-4 years) were selected and ejaculate from each ram was divided into 3 aliquots and diluted with SC, T and C extenders (final concentration - 400 million spermatozoa/0.2 ml semen) and stored at 5ºC. Semen evaluation was done by computer assisted semen analyser (CASA). Analysis of Variance (ANOVA) was used to test the significance difference between extenders. S: Sodium citrate (SC) and Tris (T) - Egg yolk extender was significantly (P<0.05) better as compared to Coconut milk extender (C). No significant difference (P>0.05) was observed between SC and T. Sperm kinematics assessment by CASA revealed Sodium citrate-based egg-yolk extender (SC) as better extender for preservation of Deccani Ram semen upto 48 hr. of storage as compared to T and C.

Keywords

Computer assisted semen analysis Liquid preservation Ram semen Sperm kinematics

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