Banner
Current IssueEditorial BoardOnline First Articles
Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Research Article

Indian Journal of Animal Research, volume 52 issue 12 (december 2018) : 1695-1700

Computer assisted semen analysis of deccani ram semen presevability at 5ºC

M. Rajashri, K. Ramchandra Reddy, G. Aruna Kumari, N. Nalini Kumari, G. Srinivas
1Department of Veterinary Gynaecology and Obstetrics, C.V.Sc, P.V. Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad-500 030, Telangana, India
Cite article:- Rajashri M., Reddy Ramchandra K., Kumari Aruna G., Kumari Nalini N., Srinivas G. (2018). Computer assisted semen analysis of deccani ram semen presevability at 5ºC. Indian Journal of Animal Research. 52(12): 1695-1700. doi: 10.18805/ijar.B-3450.

ABSTRACT

To investigate the preservability of Deccani ram semen in three different extenders - SC (Sodium Citrate), T (Tris), C (Coconut milk) egg yolk extenders after dilution and chilling (5ºC) at 1, 24 and 48 hr. of storage. Eight Deccani adult rams (aged 2-4 years) were selected and ejaculate from each ram was divided into 3 aliquots and diluted with SC, T and C extenders (final concentration - 400 million spermatozoa/0.2 ml semen) and stored at 5ºC. Semen evaluation was done by computer assisted semen analyser (CASA). Analysis of Variance (ANOVA) was used to test the significance difference between extenders. S: Sodium citrate (SC) and Tris (T) - Egg yolk extender was significantly (P<0.05) better as compared to Coconut milk extender (C). No significant difference (P>0.05) was observed between SC and T. Sperm kinematics assessment by CASA revealed Sodium citrate-based egg-yolk extender (SC) as better extender for preservation of Deccani Ram semen upto 48 hr. of storage as compared to T and C.

REFERENCES

  1. Aguirre, V., Orihuela, A. and Vazquez, R. (2007). Effect of semen collection frequency on seasonal variation in sexual behaviour, testosterone, testicular size and semen characteristics of tropical hair rams (Ovis aries). Tropical Animal Health and Production, 39: 271-7.
  2. Albiaty, N.M.H., Alobaidi, H.J.K., Kareem, A.F., Al-Hakim, A.M., Alnaeb, A.Y. and Alkhazraji, A.A.H. (2016). Effect of extenders and preservation periods in some semen characteristics of awassi rams. World Journal of Pharmaceutical Research, 5 (2): 234-43.
  3. Alcay, S., Toker, B., Ustuner, B., Nur, Z., Sagirkaya, H. and Soylu M.K. (2014). Investigation of relationships between DNA integrity and fresh semen parameters in rams. Kafkas Univ Vet Fak Derg, 20 (5): 793-798. 
  4. Azizunnesa, Z., Ohara, B.F., Bari, F.Y. and Alam, G.S. (2014). Baseline study of reproductive performances of indigenous rams in Bangladesh. IOSR Journal of Agriculture and Veterinary Science, 7: 83-89.
  5. Cancel, A., Lobdell, D., Mendola, P. and Perreault, S. (2000). Objective evaluation of hyperactivated motility in rat spermatozoa using computer assisted sperm analysis. Hum. Reprod, 15:1322- 1328.
  6. Chella, L., Kunene, N. and Lehloenya, K. (2017). A comparative study on the quality of semen from Zulu rams at various ages and during different seasons in KwaZulu-Natal, South Africa. Small Ruminant Research, 151: 104-109. http://dx.doi.org/10.1016/    j.smallrumres.2017.04.003.
  7. Freour, T., Jean, M., Mirallie, S. and Barriere, P. (2012). Computer-assisted sperm analysis parameters in young fertile sperm donors and relationship with age. Syst. Biol. Reprod Med, 58: 102–106.
  8. Freour, T., Jean, M., Mirallie, S., Dubourdieu, S. and Paul Barriere. (2010). Computer-Assisted Sperm Analysis (CASA) parameters and their evolution during preparation as predictors of pregnancy in intrauterine insemination with frozen-thawed donor semen cycles. European Journal of Obstetrics & Gynecology and Reproductive Biology, 149: 186–189.
  9. Gadea J. (2003). Semen extenders used in the artificial insemination of swine. A review. Span J Agric Res, 1:17–27.
  10. Jayaganthan, P., Perumal, P., Balamurugan, T.C. and Verma, R.P. (2015). Effect of Tinospora cordifolia supplementation on sexual behaviour and semen production in Muzzafarnagari rams. Indian J. Anim. Res, 49 (1): 140-142.
  11. Jeyendran, R.S., Vander Ven, H.H. and Perez-Pelaez, M. (1984). Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil, 70:219-228. 
  12. Jones, J.M., Lorton, S.P. and Bavister, B.D. (1995). Measurement of intracellular pH in mammalian sperm cells under physiological conditions. Cytometry, 3:235–242.
  13. Kasimanickam, R., Kasimanickam, V., Tibary, A. and Pelzer, K. (2011). Effect of semen extenders on sperm parameters of ram semen during liquid storage at 4°C. Small Rumin Res, 99: 208–213.
  14. Kasimanickam, R., Kasimanickam, V., Tibary, A. and Pelzer, K. (2007). Effect of breed and sperm concentration on the changes in structural, functional and motility parameters of ram-lamb spermatozoa during storage at 4ºC. Animal Reproduction Science, 101: 60–73.
  15. Kasimanickam, R., Nebel, R.L., Peeler, I.D., Silvia, W.L., Wolf, K.T., McAllister, A.J., Cassell, B.G., (2006). Breed differences in competitive indices of Holstein and Jersey bulls and their association with sperm DNA fragmentation index and plasma membrane integrity. Theriogenology, 66: 1307-1315.
  16. Larsen, L., Scheike, T. and Jensen, T.K. (2000). Computer-assisted semen analysis parameters as predictors for fertility of the general population. Hum Reprod, 15:1562–1567.
  17. Mircu, C., Cernescu, H., Igna, V., Knop, R., Frunza, I., Ardelean, V., Bonca, G., Otava, G., et al.(2008). Boar semen evaluation using casa and its relation to fertility. Medicina Veterinara, 41: 203-212.
  18. Paulenz, H., Soderquist, L., Adnoy, T., Fossen, O.H. and Andersen Berg, K. (2003). Effect of milk- and TRIS-based extenders on the fertility of sheep inseminated vaginally once or twice with liquid semen. Theriogenology, 60: 759–766.
  19. Paulenz, H., Soderquist, L., Perez-Pe, R. and Andersen Berg, K. (2002). Effect of different extenders and storage temperatures on sperm viability of liquid ram semen. Theriogenology, 57:823–36.
  20. Perumal, P., Selvaraju, S. and Selvakumar. (2011). Effect of prefreeze addition of cysteine hydrochloride and reduced glutathione in semen of crossbred jersey bulls on sperm parameters and conception rates. Reproduction in Domestic Animals, 46(4): 636–641.
  21. Perumal, P., Srivastava, S.K. and Ghosh, S.K. (2014). Computer-assisted sperm analysis of freezable and non-freezable Mithun (Bosfrontalis) semen. J. Anim, 1-6. 
  22. Rai, S., Tyagi, S., Kumar, M., Karunakaran, M., Mondal, M., Mandal, A. and R. Behera. (2017). Understanding motility dynamics of crossbred bull spermatozoa when analyzed by Computer Assisted Semen Analyzer (CASA). Indian J. Anim. Res, 51:1-3.
  23. Rijsselaere, T., Van, Soom, A., Tanghe, S., Coryn, M., Maes, D. and de Kruif, A. (2005). New techniques for the assessment of canine semen quality: A review. Theriogenology, 64: 706-719.
  24. Robayo, I., Montenegro, V., Valdes, C. and Cox, J.F. (2008). CASA assessment of kinematic parameters of ram spermatozoa and their relationship to migration efficiency in ruminant cervical mucus. Reprod Dom Anim, 43: 393–399.
  25. Thuwanut, P., Chatdarong, K., Bergqvist, A.S., Söderquist, L., Thiangtum, K., Tongthainan, D. and Axner, E. (2011). The effects of antioxidants on semen traits and in vitro fertilizing ability of sperm from flat-headed Cat (Prionailurus planiceps).Theriogenology, 76: 115-125. http://dx.doi.org/10.1016/j.theriogenology.2011.01.024
  26. Tretipskul, C., Buranaamnuay, K., Koonjaenak, S., Tummaruk, P. and Techakumphu, M. (2010). The use of computer-assisted sperm analysis for discriminating series of motility pattern of frozen-thawed boar semen. Thai J. Vet. Med, 40(1): 25-30.
  27. Upreti, G.C., Oliver, J.E., Duganzich, D.M., Munday, R. and Smith, J.F. (1995). Development of a chemically defined ram semen diluent (RSD-1). Anim Reprod Sci, 37:143–157.
  28. Utt, M.D. (2016). Prediction of bull fertility. Anim Reprod Sci, 169:37-44. http://www.sciencedirect.com/science/article/pii/S0378432015300919
  29. Yaniz, J.L., Marco-Aguado, M.A., Mateos, J.A. and Santolaria, P. (2010). Bacterial contamination of ram semen, antibiotic sensitivities, and effects on sperm quality during storage at 15ºC. Anim Rep Sci, 122:142– 149.
  30. Yaniz, J.L., Mateos, J.A and Santolaria, P. (2011). Zwitterionic buffers preserve ram semen quality more efficiently than TRIS during storage at 15ºC. Small Rum Res. 95:54–60.
  31. Yaniz, J.L., Mateos, J.A. and Santolaria, P. (2012). TRIS buffer improves fluorescence yield of ram spermatozoa when evaluating membrane integrity. Microsc Res Tech, 75:520–523. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)